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| 1 | SO2 removal by NH3 gas injection: effects of temperature and moisture content显示文摘 | Hsunling B Partim B Tim C K | 1994 | Industrial & Engineering Chemistry Research1994，33，5： | 1 |
| 2 | Serum outperforms plasma in small extracellular vesicle microRNA biomarker studies of adenocarcinoma of the esophagus显示文摘BACKGROUND Circulating microRNAs(miRNAs)are potential biomarkers for many diseases.However,they can originate from non-disease specific sources,such as blood cells,and compromise the investigations for miRNA biomarkers.While small extracellular vesicles(sEVs)have been suggested to provide a purer source of circulating miRNAs for biomarkers discovery,the most suitable blood sample for sEV miRNA biomarker studies has not been defined.AIM To compare the mi RNA profiles between matched serum and plasma s EV preparations to determine their suitability for biomarker studies.METHODS Matched serum and plasma samples were obtained from 10 healthy controls and10 patients with esophageal adenocarcinoma.s EV isolates were prepared from serum and plasma using Exo Quick TM and quantified using Nano Sight.RNA was extracted from s EV preparations with the mi RNeasy Serum/Plasma kit and profiled using the Taqman Openarray q PCR.The overall mi RNA content and theexpression of specific mi RNAs of reported vesicular and non-vesicular origins were compared between serum and plasma s EV preparations.The diagnostic performance of a previously identified multi-mi RNA biomarker panel for esophageal adenocarcinoma was also compared.RESULTS The overall mi RNA content was higher in plasma s EV preparations(480 mi RNAs)and contained 97.5%of the mi RNAs found in the serum s EV preparations(412 mi RNAs).The expression of commonly expressed mi RNAs was highly correlated(Spearman’s R=0.87,P<0.0001)between the plasma and serum s EV preparations,but was consistently higher in the plasma s EV preparations.Specific blood-cell mi RNAs(hsa-mi R-223-3 p,hsa-mi R-451 a,mi R-19 b-3 p,hsa-mi R-17-5 p,hsa-mi R-30 b-5 p,hsa-mi R-106 a-5 p,hsa-mi R-150-5 p and hsa-mi R-92 a-3 p)were expressed at 2.7 to 9.6 fold higher levels in the plasma s EV preparations compared to serum s EV preparations(P<0.05).In plasma s EV preparations,the percentage of protein-associated mi RNAs expressed at relatively higher levels(Ct 20-25)was greater than serum s EV preparations(50%vs 31%).While the percentage of vesicle-associated mi RNAs expressed at relatively higher levels was greater in the serum s EV preparations than plasma s EV preparations(70%vs 44%).A 5-mi RNA biomarker panel produced a higher cross validated accuracy for discriminating patients with esophageal adenocarcinoma from healthy controls using serum s EV preparations compared with plasma s EV preparations(AUROC 0.80 vs 0.54,P<0.05).CONCLUSION Although plasma s EV preparations contained more mi RNAs than serum s EV preparations,they also contained more mi RNAs from non-vesicle origins.Serum appears to be more suitable than plasma for s EV mi RNAs biomarkers studies. | Karen Chiam George C Mayne Tingting Wang David I Watson Tanya S Irvine Tim Bright Lorelle T Smith Imogen A Ball Joanne M Bowen Dorothy M Keefe Sarah K Thompson Damian J Hussey | 2020 | World Journal of Gastroenterology2020，26，20： | 1 |
| 3 | Compositional Factors Affecting NOx Emissions form Ohio Coals 显示文摘 | Kyung S J Tim C K Khang S | 2001 | Fuel Processing Technology2001，，74： | 1 |
| 4 | Co-digestion to support low temperature anaerobic pretreatment of municipal sewage in a UASB-digester显示文摘 | Lei Zhang Tim L G Hendrickx C K | 2013 | Bioresource Technology2013，148，： | 1 |
| 5 | Classifying compliance gaining messages: Taxonomic disorder and strategic confusion显示文摘 | Kellerman K and Tim C | 1994 | Communication Theory1994，4，1： | 1 |
| 6 | Evaluation of antidotes for poisoning by organophosphorus pesticides显示文摘 | Dag Jacobsen Tim J Meredith Peter Eyer Andrew J Heath David A Ligtenstein Tim C Marrs Ladislaus Szinicz J Allister Vale and John A Haines | 2000 | Emergency Medicine2000，，12： | 1 |
| 7 | Experimental investigation of flame/solid interactions in turbulent premixed combustion 显示文摘 | Ibrahim Salah S Hargrave Graham K Williams Tim C | 2001 | Experimental Thermal and Fluid Science2001，24，3： | 1 |
| 8 | Enhancing a Delphi study on family-focused prevention显示文摘 | Tim C E E Holly P K | 2007 | Technological Forecasting and Social Change2007，74，4： | 1 |
| 9 | Microbial production and modification of gases in sedimentary basins:a geochemical case study from a Devonian shale gas play,Michigan Basin显示文摘 | Martini A M Lynn M W Tim C W K | | 0，，08： | 1 |
| 10 | CDCP1 is a novel marker for hematopoietic stem cells显示文摘 | Tim C Reiner L Selim K | 2003 | Ann N Y Acad Sei2003，996，： | 1 |
| 11 | Detecting Taxus poisoning inhorses using liquid chromatography/mass spectrometry显示文摘 | Geoffrey C K Tim J L Elizabeth A D | 2000 | Vet HumToxico2000，42，3： | 1 |
| 12 | Removal of diesel particular matter(DPM) in a tubular wet electrostatic pre- cipitator显示文摘 | Phirun S Tim C K Soon-Jai K | 2007 | Journal of Electrostatics2007，65，1011： | 1 |
| 13 | CDCP1 is a novel marker for hematopoietic stem cells 显示文摘 | Tim C Reiner L Selim K | 2003 | Ann N Y Acad Sci2003，996，： | 1 |
| 14 | The effect of prehospital time related variables on mortality following severe thoracic trauma显示文摘 | Emaddin K George K Tim C | 2012 | Injury2012，43，9： | 1 |
| 15 | A model for dry sodium bicarbonate duct injection flue gas desulfurization显示文摘 | WU C G KHANG S J TIM C K | 2004 | Advances in Environmental Research2004，，8： | 1 |
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