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| 1 | Study on Endophytic Bacteria Diversity in the Root of Peperomia dindygulensis Miq. by Applying Clone Library Construction Method显示文摘[Objectives] To study the endophytic bacteria diversity in the root of Peperomia dindygulensis Miq. [Methods] The root of P.dindygulensis Miq. was taken as materials,culture-independent method was applied to build 16 S rDNA clone library for endophytic bacteria of P. dindygulensis Miq. [Results] In the 16 S rDNA clone library for endophytic bacteria of P. dindygulensis Miq.,95 clones separately belonged to 46 OTUs,Phylogenetic analysis indicated that these cloning sequences separately belonged to phylum Proteobacteria,phylum Bacteroidetes,phylum Actinobacteria,phylum Nitrospirae,and phylum Gemmatimonadetes. In these,phylum Proteobacteria accounted for 70. 53% of total number of clones,so it was the most dominant group in this library. [Conclusions]The endophytic bacteria diversity in the root of P.dindygulensis Miq. is rich and this study is expected to provide scientific basis for protection,development and use of resources of P. dindygulensis Miq. | Song JIN Zebin CHEN Yuchuan LI Tiyuan XIA Zhen REN Lifang YIN | 2017 | Medicinal Plant2017，8，6： | 1 |
| 2 | Community Structure of Endophytic Bacteria in Chestnut, Castanea mollissima显示文摘[Objective]The paper was to detect the species of endophytic bacteria in Chestnut (Castanea mollissima).[Method]The 16S rDNA-V4 region of endophytic bacteria in chestnut(sample name BL) was sequenced by Illumina Mi Seq high-throughput sequencing technology. The number of sequences and operational taxonomic units(OTUs) for each sample was sorted and calculated using Qiime and Mothur software. The abundance and α-diversity of species were analyzed.[Result]The number of effective sequences and OTUs were 21 167 /191. The rarefaction curves showed that adequate sampling was achieved,and the number of OTUs was close to saturation. The endophytic bacteria of chestnut belonged to 10 genera,including Koribacter(3. 48%),Solibacter(2. 59%),Bradyrhizobium(3. 57%), Rhodoplanes(7. 41%), Methylobacterium(4. 20%), Agrobacterium(19. 73%), Kaistobacter( 2. 05%), Sphingomonas(18. 66%),Acidovorax(35. 98%),and Methylibium(2. 32%). The dominant species were Acidovorax,Agrobacterium and Sphingomonas.[Conclusion]Illumina high-throughput sequencing technology provided more accurate and scientific data resources for the study of endophytic bacteria in chestnut. | Chen Zebin Li Bing Xia Tiyuan Wang Dingkang Yu Lei Xu Shengguang Liu Jiani Zhang Yongfu | 2016 | Plant Diseases and Pests2016，7，4： | 0 |
| 3 | Analysis on Composition and Diversity of Endophytes in Moringa oleifera显示文摘[Objectives] To explore the diversity of endophytes in Moringa oleifera,and provide a scientific basis for better protection,development and use of Moringa oleifera resources. [Methods] With Moringa oleifera stems and leaves as materials,the Illumina Mi Seq highthroughput sequencing technology was used to study the endophyte diversity. [Results] A total of 199 OTUs were measured in the samples,belonging to 12 phyla such as Proteobacteria,Actinobacteria,Acidobacteria,Gemmatimonadetes,Bacteroidetes,Chloroflexi,DeinococcusThermus,Verrucomicrobia,Candidatus Saccharibacteria and Cyanobacteria. [Conclusions]Gemmatimonas is the dominant population of endophyte in Moringa oleifera. | Song JIN Zebin CHEN Tiyuan XIA Yuchuan LI Feng ZHAO Zhen REN | 2017 | Medicinal Plant2017，8，1： | 0 |
| 4 | Transmembrane domain of IFITM3 is responsible for its interaction with influenza virus HA_(2) subunit显示文摘Interferon-inducible transmembrane protein 3(IFITM3)inhibits influenza virus infection by blocking viral membrane fusion,but the exact mechanism remains elusive.Here,we investigated the function and key region of IFITM3 in blocking influenza virus entry mediated by hemagglutinin(HA).The restriction of IFITM3 on HAmediated viral entry was confirmed by pseudovirus harboring HA protein from H5 and H7 influenza viruses.Subcellular co-localization and immunocoprecipitation analyses revealed that IFITM3 partially co-located with the full-length HA protein and could directly interact with HA_(2) subunit but not HA_(1) subunit of H5 and H7 virus.Truncated analyses showed that the transmembrane domain of the IFITM3 and HA_(2) subunit might play an important role in their interaction.Finally,this interaction of IFITM3 was also verified with HA_(2) subunits from other subtypes of influenza A virus and influenza B virus.Overall,our data demonstrate for the first time a direct interaction between IFITM3 and influenza HA protein via the transmembrane domain,providing a new perspective for further exploring the biological significance of IFITM3 restriction on influenza virus infection or HA-mediated antagonism or escape. | Wang Xu Yuhang Wang Letian Li Xiaoyun Qu Quan Liu Tiyuan Li Shipin Wu Ming Liao Ningyi Jin Shouwen Du Chang Li | 2022 | Virologica Sinica2022，37，5： | 0 |
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