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| 1 | Identification of SNPs and expression patterns of FZD3 gene and its effect on wool traits in Chinese Merino sheep(Xinjiang Type)显示文摘As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits are not clear. The objectives of this study were to identify the single nucleotide polymorphisms (SNPs) and the expression patterns of FZD3 gene, and then to determine whether it affected wool traits of Chinese Merino sheep (Xinjiang Type) or not. PCR-single stranded conformational polymorphism (PCR-SSCP) and sequencing were used to identify mutation loci, and general linear model (GLM) with SAS 9.1 was used for the association analysis between wool traits and SNPs. Quantitative real-time PCR (qRT-PCR) was used to investigate FZD3 gene expression levels. The results showed that six exons of FZD3 gene were amplified and two mutation loci were identified in exon 1 (NC_019459.2: g.101771685 T>C (SNP1)) and exon 3 (NC_019459.2: g.101810848, A>C (SNP2)), respectively. Association analysis showed that SNP1 was significantly associated with mean fiber diameter (MFD)(P=0.04) and live weight (LW)(P=0.0004), SNP2 was significantly associated with greasy fleece weight (GFW)(P=0.04). The expression level of FZD3 gene in skin tissues of the superfine wool (SF) group was significantly lower (P<0.05) than that of the fine wool (F) group. Moreover, it had a higher expression level (P<0.01) in skin tissues than in other tissues of Chinese Merino ewes. While, its expression level had a fluctuant expression in skin tissues at different developmental stages of embryos and born lambs, with the highest expression levels (P<0.01) at the 65th day of embryos. Our study revealed the genetic relationship between FZD3 variants and wool traits and two identified SNPs might serve as potential and valuable genetic markers for sheep breeding and lay a molecular genetic foundation for sheep marker-assisted selection (MAS). | ZHAO Bing-ru FU Xue-feng TIAN Ke-chuan HUANG Xi-xia DI Jiang BAI Yan XU Xin-ming TIAN Yue-zhen WU Wei-wei ABLAT Sulayman ZENG Wei-dan HANIKEZI Tulafu | 2019 | Journal of Integrative Agriculture2019，18，10： | 2 |
| 2 | Genome Array on Differentially Expressed Genes of Skin Tissue in Cashmere Goat at Early Anagen of Cashmere Growth Cycle Using DNA Microarray显示文摘In order to study the molecular mechanism involved in cashmere regeneration, this study investigated the gene expression profile of skin tissue at various stages of the cashmere growth cycle and screen differentially expressed genes at proangen in 10 cashmere goats at 2 years of age using agilent sheep oligo microarray. Significance analysis of microarray(SAM) methods was used to identify the differentially expressed genes, Hierarchical clustering was performed to clarify these genes in association with different cashmere growth stages, and GO(Gene ontology) and the pathway analyses were con-ducted by a free web-based Molecular Annotation System3.0(MAS 3.0). Approximately 10 200 probe sets were detected in skin tissue of 2-yr-old cashmere goat. After SAM analysis of the microarray data, totally 417 genes were shown to be differentially expressed at different cashmere growth stages, and 24 genes are significantly up-regulated(21) or down-regulated(3) at proangen concurrently compared to angen and telogen. Hierarchical clustering analysis clearly distinguished the differentially expressed genes of each stage. GO analysis indicated that these altered genes at proangen were predominantly involved in collagen fibril organization, integrin-mediated signaling pathway, cell-matrix adhesion, cell adhesion, transforming growth factor-β(TGF-β) receptor signaling pathway, regulation of cell growth. Kyoto encyclopedia of genes and genomes(KEGG) analysis showed that the significant pathways involved mainly included focal adhesion and extracellular matrixc(ECM)-receptor interaction. Some important genes involved in these biological processes, such as COL1A1, COL1A2, COL3A1, SPARC, CYR61 and CTGF, were related to tissue remolding and repairing and detected by more than one probe with similar expression trends at different stages of cashmere growth cycle. The different expression of these genes may contribute to understanding the molecular mechanism of cashmere regeneration. | DI Jiang XU Xin-ming Lazate Ainiwaer ZHANG Yan-hua TIAN Ke-chuan YU Li-juan WU Wei-wei Hanikezi Tulafu FU Xue-feng Marzeya Yasen | 2014 | Journal of Integrative Agriculture2014，13，10： | 2 |
| 3 | Effects of atrial natriuretic peptide on inter-organ crosstalk among the kidney, lung, and heart in a rat model of renal ischemia-reperfusion injury显示文摘 | Chieko Mitaka May Khin Hnin Si Miniwan Tulafu Qi Yu Tokujiro Uchida Shinya Abe Masanobu Kitagawa Satoshi Ikeda Yoshinobu Eishi Makoto Tomita | 2014 | Intensive Care Medicine Experimental2014，，1： | 1 |
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