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| 1 | Effect of probiotic Lactobacillus plantarum Dad-13 powder consumption on the gut microbiota and intestinal health of overweight adults显示文摘BACKGROUND Shifting on lifestyle,diet,and physical activity contributed on increasing number of obese people around the world.Multiple factors influence the development of obesity.Some research suggested that gut microbiota(GM)plays an important role in nutrient absorption and energy regulation of individuals,thus affecting their nutritional status.Report of Indonesia Basic Health Research showed that the prevalence of obesity in every province tended to increase.Although the root cause of obesity is excessive calorie intake compared with expenditure,the differences in gut microbial ecology between healthy and obese humans may affect energy homeostasis.GM affect body weight,especially obesity.Probiotics that are consumed while alive and able to colonize in the intestine are expected to increase the population of good bacteria,especially Bifidobacteria and Lactobacilli,and suppress pathogens such as Enterobacteriaceae and Staphylococcus.The strain of L.plantarum Dad-13 has been demonstrated to survive and colonize in the gastrointestinal tract of healthy Indonesian adults who consume fermented milk containing L.plantarum Dad-13.The consumption of probiotic L.plantarum Dad-13 powder decreased E.coli and non-E.coli coliform bacteria in school-aged children in Indonesia.L.plantarum is a dominant bacterium in the average Indonesian’s GM.For this reason,this bacterium is probably a more suitable probiotic for Indonesians.AIM To determine the effect of the consumption of indigenous probiotic Lactobacillus plantarum Dad-13 powder in overweight adults in Yogyakarta(Indonesia).METHODS Sixty overweight volunteers with a body mass index(BMI)equal to or greater than 25 consume indigenous probiotic powder L.plantarum Dad-13(2×109 CFU/gram/sachet)for 90 d.The study was a randomized,double-blind,placebocontrolled study.The volunteers filled in a diary on a daily basis,which consisted of questions on study product intake(only during ingestion period),other food intake,number of bowel movements,fecal quality(consistency and color),any medications received,and any symptom of discomfort,such as diarrhea,constipation,vomiting,gassing,sensation of illness,etc.Fecal samples and the subjects’diaries were collected on the morning of day 10+1,which was marked as the end of the baseline period and the start of the ingestion period.During the ingestion period(from day 11 to day 101),several parameters to measure and analyze the results included body weight and height(once a month),the lipid profile,GM analysis using MiSeq,short-chain fatty acid(SCFA)analysis using gas chromatography,and the measurement of fecal pH using a pH meter.RESULTS The consumption of indigenous probiotic powder L.plantarum Dad-13 caused the average body weight and BMI of the probiotic group to decrease from 84.54±17.64 kg to 83.14±14.71 kg and 33.10±6.15 kg/m2 to 32.57±5.01 kg/m2,respectively.No significant reduction of body weight and BMI in the placebo group was observed.An analysis of the microbiota showed that the number of Bacteroidetes,specifically Prevotella,increased significantly,while that of Firmicutes significantly decreased.No significant change in lipid profile in both groups was found.Also,no significant change in SCFAs(e.g.,butyrate,propionate,acetic acid)and pH level was found after the consumption of the probiotic.CONCLUSION No significant differences in pH before and after ingestion were observed in both the probiotic and placebo groups as well as in the lipid profile of both cholesterol and triglyceride,high-density lipoprotein(HDL),low-density lipoprotein(LDL),and the LDL/HDL ratio.In addition,no significant changes in the concentration of SCFAs(e.g.,acetic acid,propionate,and butyrate)were found after consumption.Interestingly,a significant decrease in body weight and BMI(P<0.05)was determined in the treatment group.An analysis of GM shows that L.plantarum Dad-13 caused the Firmicutes population to decrease and the Bacteroidetes population(especially Prevotella)to increase. | Endang Sutriswati Rahayu Mariyatun Mariyatun Nancy Eka Putri Manurung Pratama Nur Hasan Phatthanaphong Therdtatha Riko Mishima Husnita Komalasari Nurul Ain Mahfuzah Fathyah Hanum Pamungkaningtyas Wahyu Krisna Yoga Dina Aulia Nurfiana Stefanie Yolanda Liwan Mohammad Juffrie Agung Endro Nugroho Tyas Utami | 2021 | World Journal of Gastroenterology2021，27，1： | 6 |
| 2 | Gut microbiota profile in healthy Indonesians显示文摘BACKGROUND Recently, gut microbiota has been associated with various diseases other than intestinal disease. Thus, there has been rapid growth in the study of gut microbiota. Considering the numerous factors influencing gut microbiota such as age, diet, etc., area-based research is required. Indonesia has numerous different tribes and each of these tribes have different lifestyles. Hence, it is expected that each tribe has a specific gut microbiota. A deeper insight into the composition of gut microbiota can be used to determine the condition of gut microbiota in Indonesians and to consider which treatment may be suitable and effective to improve health status.AIM To investigate the gut microbiota of Indonesian subjects represented by Javanese and Balinese tribes by analyzing fecal samples.METHODS Fecal samples were collected from a total of 80 individuals with 20 in each of the young groups ranging from 25-45 years and the elderly group aged 70 years or more from two different regions, Yogyakarta and Bali. Fecal sample collection was performed at the end of the assessment period(day 14 ± 1 d) during which time the subjects were not allowed to consume probiotic or antibiotic products.The quantification of various Clostridium subgroups, Lactobacillus subgroups,Enterococcus, Streptococcus, Staphylococcus, Bacteroides fragilis group and Prevotella,Bifidobacterium and Atopobium cluster, Enterobacteriaceae and Pseudomonas was performed using the Yakult intestinal flora-scan(YIF-SCAN).RESULTS The bacterial population in younger subjects' feces was higher than that in the elderly population, with a total of approximately 10.0 – 10.6 log10 bacterial cells/g feces. The most abundant bacteria in all groups were Clostridium, followed by Prevotella, Atopobium, Bifidobacterium and Bacteroides. In the elderly, an increase in Enterobacteriaceae, Coliform and Escherichia coli was found. In terms of bacterial counts in Yogyakarta, total bacteria, Clostridium coccoides(C. coccoides) group,Bifidobacterium, Prevotella, Lactobacillus plantarum subgroup, and Streptococcus were significantly higher(P < 0.05) in younger than elderly subjects, while the Lactobacillus gasseri subgroup, Lactobacillus casei subgroup, and Lactobacillus reuteri subgroup counts were significantly lower(P < 0.05) in younger subjects. In Balinese subjects, total bacteria, C. coccoides group, Clostridium leptum subgroup,Bacteroides fragilis group, and Prevotella were significantly higher(P < 0.05) in younger compared to elderly individuals, while the Lactobacillus ruminis subgroup, and Enterobacteriaceae were significantly lower(P < 0.05) in younger subjects. The results also revealed that, besides the C. coccoides group and Clostridium leptum group being the most abundant gut microbiota in both Yogyakarta and Balinese people, the latter was indicated by a higher Clostridium perfringens count, which was almost 10 times that of Yogyakarta subjects. This may be a response to different lifestyles in the different tribes; however, this phenomenon requires further extensive study.CONCLUSION Bacterial populations were higher in younger than in elderly subjects. Most abundant bacterial groups were Clostridium, Prevotella, Atopobium, Bifidobacterium,and Bacteroides. The level of Clostridium perfringens in Yogyakarta subjects was lower than that in Balinese subjects. | Endang Sutriswati Rahayu Tyas Utami Mariyatun Mariyatun Pratama Nur Hasan Rafli Zulfa Kamil Ryan Haryo Setyawan Fathyah Hanum Pamungkaningtyas IskANDar Azmy Harahap Devin Varian Wiryohanjoyo Putrika Citta Pramesi Muhammad Nur Cahyanto I Nengah Sujaya Mohammad Juffrie | 2019 | World Journal of Gastroenterology2019，25，12： | 6 |
| 3 | Concurrent infections of dengue viruses serotype 2 and 3 in patient with severe dengue from Jakarta, Indonesia显示文摘Objective: To describe the clinical manifestation of patient with severe dengue, to identify the serotypes and genotypes of dengue viruses(DENV) which concurrently infecting the patient, and to explore the possible relationship of severe dengue with the concurrent infection of DENV. Methods: Dengue diagnosis was performed using NS1 antigen detection and Ig G/Ig M ELISA. Standard clinical and laboratory examinations were performed to obtain the clinical and hematological data. DENV concurrent infections were detected and confirmed using RT-PCR and DENV Envelope gene sequencing. Phylogenetic analyses were performed to determine the genotypes of the viruses. Results: The patient was classified as having severe dengue characterized by severe plasma leakage, hemorrhage, and organ damage involving lung, liver, and kidney. Concurrent infection of DENV serotype 2 and 3 was observed. The infecting DENV-2 virus was grouped into Cosmopolitan genotype while DENV-3 virus was classified into Genotype Ⅰ. Both viruses were closely related to isolates that were endemic in Jakarta. Viremia measurement was conducted and revealed a significantly higher virus titer of DENV-3 compared to DENV-2. Conclusions: The occurrence of multi-serotype DENV infections was presented in a patient with severe clinical manifestation in Indonesia. The hyperendemicity of dengue in Indonesia may contribute to the DENV concurrent infections cases and may underlie the severity of the disease. | Soroy Lardo Yaldiera Utami Benediktus Yohan Seri MMU Tarigan Widayat Djoko Santoso Leonard Nainggolan R Tedjo Sasmono | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，2： | 3 |
| 4 | Effect of sucrose and potassium nitrate on biomass and saponin content of Talinum paniculatum Gaertn. hairy root in balloon-type bubble bioreactor显示文摘Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of T.paniculatum were infected by Agrobacterium rhizogenes strain LB510.The hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L BTBB.Each BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).Cultures were maintained for 14days.Fresh and dry weights of hairy roots at the end of culture were investigated.Results:Various concentrations of sucrose influenced the biomass accumulation of hairy roots.Maximum biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than control.Culture supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than control.However,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of MS.Conclusions:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of T.paniculatum in the large scale. | Yosephine Sri Wulan Manuhara Alfinda Novi Kristanti Edy Setiti Wida Utami Arif Yachya | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，12： | 3 |
| 5 | Antileukemic prop- erties of Spermacoce species 显示文摘 | Sukari M A Utami R Neoh B K | 2013 | Asian Journal of Chemistry2013，25，8： | 1 |
| 6 | The nucleolar GTP-bindingproteins Gnl2 and nucleostemin are required for retinal neurogenesis indeveloping zebrafish显示文摘 | Paridaen JT Janson E Utami KH | 2011 | Dev Biol2011，355，2： | 1 |
| 7 | Microsat- ellite markers for population studies of the rice blast fungus, Magnaporthe grisea 显示文摘 | Adreit H Andriantsimialona D Utami DW | 2007 | Molecular Ecology Notes2007，7，4： | 1 |
| 8 | Detoxification of phytotoxic compounds by Ti02 photocatalysis in a recy-cling hydroponic cultivation system of asparagus显示文摘 | Sun ada K Ding X G Utami M S | 2008 | Journal of Agriculture and Food Chemistry2008，56，： | 1 |
| 9 | Detoxification of phytotoxic compounds by TiO2 photocatalysis in a recycling hydroponic cultivation system of asparagus显示文摘 | Sunada K Ding X G Utami M S | 2008 | J Agric Food Chem2008，56，： | 1 |
| 10 | A multi-samples, multi-extraets approaeh for mierosatellite analysis of faecal samplesin an arboreal ape 显示文摘 | Goossens B Chikhi L Utami S S | 2000 | Conservation Genetics2000，1，2： | 1 |
| 11 | Detoxification of phytotoxic compounds by TiO2 photocatalysis in a recycling hydroponic cultivation system of asparagus 显示文摘 | Sunada K Ding X G Utami M S | 2008 | Journal of Agricultural and Food Chemistry2008，56，： | 1 |
| 12 | A multi - samples, multiextracts approach for microsatellite analysis of faecal samples in an arboreal ape显示文摘 | Goossens B Chikhi L Utami S S | 2000 | Conservation Genetics2000，1，： | 1 |
| 13 | Detoxification of phytotoxic compounds by TiO2 photocatalysis in a recycling hydroponic cultivation system of asparagus显示文摘 | Sunada K Ding X G Utami M S | 2008 | Journal of Agricultural and FoodChemistry2008，56，12： | 1 |
| 14 | The inhibition of Typhonium flagelliforme Lodd. Blume leaf extract on COX-2 expression of Wi Dr colon cancer cells显示文摘Objective: To determine the inhibition activity of Typhonium flagelliforme Lodd. Blume(T. flagelliforme) leaf extract on cyclooxygenase 2(COX-2) expression of colon cancer cells.Methods: T. flagelliforme leaf extract was prepared to macerate in ethyl acetate. In vitro anticancer activity was assayed by MTT method on Wi Dr colon cancer cells. This study applied apoptosis induction assay to investigate the mechanism of cell death using double staining method. COX-2 expression was stained by immunocytochemistry.Results: T. flagelliforme showed anticancer activity and induced apoptosis on Wi Dr cells through inhibition of COX-2 expression with IC5070 mg/m L.Conclusions: This study showed that T. flagelliforme is a promising chemopreventive agent for colon cancer through COX-2 inhibition. | Agustina Setiawati Handika Immanuel Mery Tri Utami | 2016 | Asian Pacific Journal of Tropical Biomedicine2016，6，3： | 1 |
| 15 | ldentification of novel candidate genes associated with cleft lip and palate using array comparative genomic hybridisation显示文摘 | OSOEGAWA K VESSERE G M UTAMI K H | 2008 | J Med Genet2008，45，2： | 1 |
| 16 | A Multi-sampies, Multi-extracts Approach for Microsatellite Analysis of Faecal in an Arboreal Ape 显示文摘 | GOOSSENS B CHIKHI L UTAMI S S | 2000 | Conservation Genetics2000，1，： | 1 |
| 17 | Biodiversity of Indigenous Amylolytic and Cellulolytic Bacteria in Sago Waste Product at Susupu, North Moluccas显示文摘 | Utami Sri Hastuti Pramitha Yakub Henny Nurul Khasanah | 2014 | Journal of Life Sciences2014，8，11： | 1 |
| 18 | The effect of giving kombucha tea on stability of blood serum pH of Rattus norvegicus 显示文摘 | Utami E R Suyono D | 2012 | UNESA Journal of Chemistry2012，2，1： | 1 |
| 19 | Detoxification of phytotoxic compounds by TiO2 photocatalysis in a recycling hydroponic cultivation system of asparagus显示文摘 | Sunada K Ding X G Utami M S | | 0，，12： | 1 |
| 20 | Systematic implication of seed coat morphology in some Impatiens species (Balsaminaceae) 显示文摘 | UTAMI N SHIMIZU T | 1996 | Journal Phytogeography &Taxon1996，43，： | 1 |
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