|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Stress- and Pathogen-Induced Arabidopsis WRKY48 is a Transcriptional Activator that Represses Plant Basal Defense显示文摘植物 WRKY 抄写因素能作为植物的积极或否定的管理者工作基础疾病抵抗。Arabidopsis WRKY48 被机械或渗透的应力由于渗入和病原体感染导致并且因此,可以在植物防卫回答起一个作用。WRKY48 对原子核局部性,在 vitro 与一种高亲密关系认出 TTGACC W 盒子顺序并且在象强壮的 transcriptional 使活跃之物的植物房间工作。直接决定生物功能,我们孤立 loss-of-function T-DNA 插入异种并且产生了转基因的 overexpression 在 Arabidopsis 为 WRKY48 种的 gain-of-function。细菌的病原体 Pseudomonas syringae 的剧毒的紧张的生长在 wrky48 T-DNA 插入异种被减少。loss-of-function 异种的提高的抵抗与水杨酸的增加的正式就职被联系由细菌的病原体的调整酸的 PR1。由对比,转基因的 WRKY48-overexpressing 植物支持 P 的提高的生长。syringae 和提高的危险性与防卫相关的 PR 基因的减少的表示被联系。这些结果建议 WRKY48 是 PR 基因表示和基础抵抗的一个否定管理者到细菌的病原体 P。syringae。 | Deng-Hui Xing Zi-Bing Lai Zu-Yu Zheng K. M. Vinod Bao-Fang Fan Zhi-Xiang Chen | 2008 | Molecular Plant2008，1，3： | 37 |
| 2 | QTL and QTL × Environment Effects on Agronomic and Nitrogen Acquisition Traits in Rice显示文摘Agricultural environments deteriorate due to excess nitrogen application. Breeding for low nitrogen responsive genotypes can reduce soil nitrogen input. Rice genotypes respond variably to soil available nitrogen. The present study attempted quantification of genotype × nitrogen level interaction and mapping of quantitative trait loci (QTLs) associated with nitrogen use efficiency (NUE) and other associated agronomic traits. Twelve parameters were observed across a set of 82 double haploid (DH) lines derived from IR64/Azucena. Three nitrogen regimes namely, native (0 kg/ha; no nitrogen applied), optimum (100 kg/ha) and high (200 kg/ha) replicated thrice were the environments. The parents and DH lines were significantly varying for all traits under different nitrogen regimes. All traits except plant height recorded significant genotype × environment interaction. Individual plant yield was positively correlated with nitrogen use efficiency and nitrogen uptake. Sixteen QTLs were detected by composite interval mapping. Eleven QTLs showed significant QTL × environment interactions. On chromosome 3, seven QTLs were detected associated with nitrogen use, plant yield and associated traits. A QTL region between markers RZ678, RZ574 and RZ284 was associated with nitrogen use and yield. This chromosomal region was enriched with expressed gene sequences of known key nitrogen assimilation genes. | Senapathy Senthilvel Kunnummal Kurungara Vinod Palaniappan Malarvizhi Marappa Maheswaran | 2008 | Journal of Integrative Plant Biology2008，50，9： | 12 |
| 3 | Biodiversity,current developments and potential biotechnological applications of phosphorus-solubilizing and-mobilizing microbes:A review显示文摘As one of the most important and essential macronutrients next to nitrogen,phosphorus(P)is important for plant development,but it is the least mobile nutrient element in plant and soil.Globally,P is mined from geological sediments and added to agricultural soils so as to meet the critical requirements of crop plants for agronomic productivity.Phosphorus exists in soil in both organic and inorganic forms.The various inorganic forms of the element in soil are salts with calcium,iron,and aluminum,whereas the organic forms come from decaying vegetation and microbial residue.There is a huge diversity of plant microbiomes(epiphytic,endophytic,and rhizospheric)and soil microbiomes that have the capability to solubilize the insoluble P and make it available to plant.The main mechanism for the solubilization of inorganic P is by the production of organic acids,which lowers soil pH,or by the production of acid and alkaline phosphatases,which causes the mineralization of organic P.The P-solubilizing and-mobilizing microorganisms belong to all three domains,comprising archaea,bacteria,and eukarya.The strains belonging to the genera Arthrobacter,Bacillus,Burkholderia,Natrinema,Pseudomonas,Rhizobium,and Serratia have been reported as efficient and potential P solubilizers.The use of P solubilizers,alone or in combination with other plant growth-promoting microbes as an eco-friendly microbial consortium,could increase the P uptake of crops,increasing their yields for agricultural and environmental sustainability. | Divjot KOUR Kusam Lata RANA Tanvir KAUR Neelam YADAV Ajar Nath YADAV Manish KUMAR Vinod KUMAR Harcharan Singh DHALIWAL Anil Kumar SAXENA | 2021 | Pedosphere2021，31，1： | 10 |
| 4 | 人HCN4通道的滞后现象:影响窦房结起搏的潜在决定因素(英文)显示文摘超极化活化环核苷酸门控(hyperpolarization-activated cyclic-nucleotide-gated,HCN)通道参与调制心脏跳动的节律和速率。与HCN1和HCN2有所不同,慢通道HCN4可能不存在电压依赖的滞后现象。本研究采用单细胞膜片钳方法,在稳定转染hHCN4的HEK293细胞上进行电生理记录,观察hHCN4通道是否存在滞后现象,以及cAMP对其的调制作用;同时采用实时定量RT-PCR方法检测窦房结和心房组织中HCNs的表达。电压钳实验结果显示hHCN4电流(Ih)激活随着保持电位超极化的变化而向去极化方向移动。三角电位变化钳(triangular ramp)和动作电位钳的结果也显示了hHCN4的滞后现象。cAMP增加Ih电流幅度,且使电流激活向去极化方向移动,从而改变内源性hHCN4滞后行为。RT-PCR结果显示,人窦房结组织主要表达HCN4,占75%,HCN1占21%,HCN2占3%,HCN3占0.7%。以上结果提示,人窦房结组织主要表达HCN4亚型,hHCN4的Ih存在电压依赖性的滞后现象,且受cAMP调制。由此推断,hHCN4通道的滞后现象可能在窦房结起搏活动中起到了关键作用。 | 萧永福 Natalie Chandler Halina Dobrzynski Eric S.Richardson Erica M.TenBroek Joshua J.Wilhelm Vinod Sharma Anthony Varghese Mark R.Boyett Paul A.Iaizzo Daniel C.Sigg | 2010 | 生理学报2010，62，1： | 10 |
| 5 | Enhanced recovery after surgery protocol enhances early postoperative recovery after pancreaticoduodenectomy显示文摘Background: Enhanced recovery after surgery(ERAS) protocol is a multimodal, multidisciplinary and evidence-based approach to reduce surgical stress and enhance recovery in the postoperative period. This study aimed to analyze the outcome of ERAS protocol in patients after pancreaticoduodenectomy(PD). Methods: A total of 50 consecutive patients with pancreatic/periampullary cancer who underwent PD between January 2016 to August 2017 were included in the study. As per the institute ERAS protocol, nasogastric tube(NGT) was removed on postoperative day(POD) 1 if output was less than 200 mL and oral sips were allowed; oral liquids were allowed on POD2; semisolid diet by POD3; abdominal drain was removed on POD 4 if output was less than 100 mL with no evidence of postoperative pancreatic fistula(POPF); normal diet was allowed on POD5. Discharge criteria on POD6 were afebrile, tolerating oral normal diet, pain free and no surgery related complications(defined as per the ISGPS definitions). Results: NGT was removed on POD1 in 45(90%) patients, abdominal drain removed by POD4 in 41(82%) and 43(86%) patients were discharged on POD6. There was no 30-day postoperative mortality. Three(6%) patients had delayed gastric emptying(DGE). None had postoperative hemorrhage and POPF. Readmission rate was 8%. A significant relation was found between the length of hospital stay(LOS) with age( P < 0.05) and a marginal relation between LOS and postoperative albumin( P = 0.05). Conclusions: ERAS protocol can be safely followed in the perioperative care of patients who undergo PD. Early removal of NGT and allowing oral diet restore bowel function early. ERAS decreases the LOS and postoperative complications. | Ramasamy Mahendran Mallika Tewari Vinod Kumar Dixit Hari Shankar Shukla | 2019 | Hepatobiliary & Pancreatic Diseases International2019，18，2： | 9 |
| 6 | SWR1 Chromatin-Remodeling Complex Subunits and H2A.Z Have Non-overlapping Functions in Immunity and Gene Regulation in Arabidopsis显示文摘进由改变建筑群的 SWR1 染色质的 nucleosomes 的 histone 变体 H2A.Z 的加入是在真核细胞的基因规定的关键步。在 Arabidopsis, SWR1c 和 H2A.Z 被显示了控制位于开发和环境回答下面的基因表示。尽管他们在防卫被含有,在免疫的复杂子单元和 H2A.Z 的特定的角色很好没被理解。在这研究,我们分析了 SWR1c 子单元,光周期无关的早 FLOWERING1 (PIE1 ) ,肌动朊相关的 PROTEIN6 (ARP6 ) ,和 SWR1 建筑群的角色 6 (SWC6 ) ,以及 H2A.Z,在防卫和基因规定。我们发现 SWR1c 部件在抵抗起不同作用到不同病原体。PIE1 和 SWC6 功能以及 H2A.Z 的弄空的损失导致了减少的基础抵抗,当 ARP6 fucntion 的损失导致了提高的抵抗时。我们发现在 PIE1 和 SWC6 的变化导致了损害被触发受动器的免疫。在 SWR1c 部件和 H2A.Z 的变化也导致了损害 jasmonic acid/ethylene-mediated 免疫。染色体宽的表示分析同样在基因规定为 H2A.Z 和 SWR1c 部件揭示不同角色,并且在在表明小径的防卫之间的生气谈话的规定为 PIE1 建议一个潜在的角色。我们数据表演尽管他们是一样的建筑群的部分, Arabidopsis SWR1c 部件能在植物免疫和基因规定有非冗余的函数。 | Souha Berriri Sreeramaiah N Gangappa and S Vinod Kumar | 2016 | Molecular Plant2016，9，7： | 7 |
| 7 | H2A.Z at the Core of Transcriptional Regulation in Plants显示文摘 | S. Vinod Kumar | 2018 | Molecular Plant2018，11，9： | 7 |
| 8 | Carbon-coated manganese dioxide nanoparticles and their enhanced electrochemical properties for zinc-ion battery applications显示文摘In this study, we report the cost-effective and simple synthesis of carbon-coated α-MnO_2nanoparticles(α-MnO_2@C) for use as cathodes of aqueous zinc-ion batteries(ZIBs) for the first time. α-MnO_2@C was prepared via a gel formation, using maleic acid(C_4H_4O_4) as the carbon source, followed by annealing at low temperature of 270 °C. A uniform carbon network among the α-MnO_2 nanoparticles was observed by transmission electron microscopy. When tested in a zinc cell, the α-MnO_2@C exhibited a high initial discharge capacity of 272 m Ah/g under 66 m A/g current density compared to 213 m Ah/g, at the same current density, displayed by the pristine sample. Further, α-MnO_2@C demonstrated superior cycleability compared to the pristine samples. This study may pave the way for the utilizing carbon-coated MnO_2 electrodes for aqueous ZIB applications and thereby contribute to realizing high performance eco-friendly batteries. | Saiful Islam Muhammad Hilmy Alfaruqi Jinju Song Sungjin Kim Duong Tung Pham Jeonggeun Jo Seokhun Kim Vinod Mathew Joseph Paul Baboo Zhiliang Xiu Jaekook Kim | 2017 | Journal of Energy Chemistry2017，26，4： | 6 |
| 9 | 仙茅:对健康有众多益处的黑金(英文)显示文摘仙茅(石蒜科)是一种濒临灭绝的多年生草本植物,是印度本土药,作为强有力的适应原和春药在印度传统药物中占有重要位置。它也是多种印度传统药物处方的重要组成部分,被认为具有促进性欲、刺激免疫、保护肝脏、抗氧化、抗癌和治疗糖尿病等作用。已有报道,从仙茅中提取出多种化学成分,如植物黏胶,酚苷、皂苷和脂肪族化合物等。仙茅也是中医和日本汉方医学中各种草药处方的重要成分。本文对仙茅的多种生物活性和其不同化学成分的作用进行了综述。 | Nagendra Singh Chauhan Vikas Sharma Mayank Thakur Vinod Kumar Dixit | 2010 | 中西医结合学报2010，8，7： | 6 |
| 10 | Repetitive Sequences in Plant Nuclear DNA:Types, Distribution, Evolution and Function显示文摘Repetitive DNA sequences are a major component of eukaryotic genomes and may account for up to 90% of the genome size. They can be divided into minisatellite, microsatellite and satellite sequences. Satellite DNA sequences are considered to be a fast-evolving component of eukaryotic genomes, comprising tandemly-arrayed, highly-repetitive and highly-conserved monomer sequences. The monomer unit of satellite DNA is 150–400 base pairs(bp) in length.Repetitive sequences may be species- or genus-specific, and may be centromeric or subtelomeric in nature. They exhibit cohesive and concerted evolution caused by molecular drive, leading to high sequence homogeneity. Repetitive sequences accumulate variations in sequence and copy number during evolution, hence they are important tools for taxonomic and phylogenetic studies, and are known as ‘‘tuning knobs' ' in the evolution. Therefore, knowledge of repetitive sequences assists our understanding of the organization, evolution and behavior of eukaryotic genomes. Repetitive sequences have cytoplasmic, cellular and developmental effects and play a role in chromosomal recombination. In the post-genomics era, with the introduction of next-generation sequencing technology, it is possible to evaluate complex genomes for analyzing repetitive sequences and deciphering the yet unknown functional potential of repetitive sequences. | Shweta Mehrotra Vinod Goyal | 2014 | Genomics, Proteomics & Bioinformatics2014，12，4： | 5 |
| 11 | Co-gasification of coal and biomass an emerging clean energy technology: Status and prospects of development in Indian context显示文摘Co-gasification of coal and biomass is emerging as potential clean fuel technology to achieve high thermodynamic efficiency with relatively low CO2 emission. The coal and biomass have been exclusively gasified more than a century to obtain gas–liquid fuels and the production of chemicals. Co-gasification has higher efficiency than the solitary coal gasification because the cellulose, hemicellulose and lignin content of biomass help to ignite and enhance the rate of gasification. It is suggested that the extensive research on carbon reactivity pattern, heat release, reaction kinetics, etc. may support to reduce the uncertainties in the co-gasification performance of coal and biomass blends, particularly in India. The prospects of co-gasification technology in Indian context have been discussed considering the abundance of varieties of coal and biomass. The suitability of existing gasifier procedures and their limitations with operating parameters like temperature, residence time, density optimisation, feed rate, agglomeration intensity, the tar formation and techno-economics involved are described. Also, this paper reviews the research highlights of the history of co-gasification and the advancement in upcoming challenges like a design of gasifier, access and preparation of biomass, disposal of residue, environmental concerns and reassurance to the operators for execution of large and small-scale projects. | Alka D.Kamble Vinod Kumar Saxena Prakash Dhondiram Chavan Vinod Atmaram Mendhe | 2019 | International Journal of Mining Science and Technology2019，29，2： | 5 |
| 12 | Treatment quality and outcome for multidrug-resistant tuberculosis patients in four regions of China: a cohort study显示文摘Background:China incurs an extremely low treatment coverage of multidrug-resistant tuberculosis(MDR-TB).This study aimed to understand the experience of MDR-TB patients on quality of health care,and the clinical impact through an up to six-year follow-up.Methods:Cohorts of MDR-TB patients were built in TB/MDR-TB designated hospitals in four regions of China from 2014 to 2015.Patients were followed up during treatment course,and yearly confirmation afterward until 2019.Delay in MDR-TB diagnosis and treatment was calculated upon bacteriological confirmation and treatment initiation.Risk factors for unfavourable outcomes were identified by multivariate logistic regression.Results:Among 1168 bacteriological-positive TB patients identified from a 12-million population,58(5.0%)MDR-TB cases were detected.The median delay for MDR-TB diagnosis was 90.0 days,with 13.8%having a delay above 180.0 days.MDR-TB treatment was only recommended to 19(32.8%)participants,while the rest continued with regimen for drug-susceptible TB.In MDR-TB treatment group,36.8%achieved treatment success,while the others had incomplete treatment(21.1%),loss to follow-up(36.8%)and TB relapse(5.3%).For non-MDR-TB treatment group,33.3%succeeded,25.6%relapsed,2.6%failed,23.1%died,and 15.4%were lost to follow-up.Overall,only 35.7%(20/56)of detected MDR-TB patients had favourable outcomes and higher education level was positively associated with it(adjusted odds ratio[aOR]:3.60,95%confidence interval[CI]:1.04-12.5).Conclusions:A large proportion of patients did not receive MDR-TB treatment and had unfavourable outcomes.Delayed MDR-TB diagnosis resulted in poor quality of MDR-TB care.Rapid diagnosis,regulated patient management and high-quality MDR-TB treatment should be enhanced in China. | Xu-Bin Zheng Vinod K.Diwan Qi Zhao Yi Hu Judith Bruchfeld Wei-Li Jiang Sven Hoffner Biao Xu | 2020 | Infectious Diseases of Poverty2020，9，4： | 5 |
| 13 | 寄生草本植物大花菟丝子的研究综述(英文)显示文摘大花菟丝子是一种金黄色、无叶、多年生寄生草本植物,它隶属于旋花科,俗称发菜、鬼肠等。经研究,大花菟丝子具有止痉、抗惊厥、抗类固醇生成、降血压、助肌肉松弛、强心、利尿、抗病毒、抗菌、抗氧化以及助毛发生长等一系列药用作用。目前从大花菟丝子中提取的有效化学成分有岩白菜素、阿马别林、β-谷甾醇、豆甾醇、山奈酚、半乳糖醇、杨梅酮、槲皮素、香豆素和齐墩果酸等。本文概述了生药学和植物化学文献中有关大花菟丝子的研究结果,并从传统医学和生物学角度评价了它的医药用途。 | Satish Patel Vikas Sharma Nagendra S.Chauhan Vinod K.Dixit | 2012 | 中西医结合学报2012，10，3： | 5 |
| 14 | A Local Contrast Fusion Based 3D Otsu Algorithm for Multilevel Image Segmentation显示文摘To overcome the shortcomings of 1 D and 2 D Otsu’s thresholding techniques, the 3 D Otsu method has been developed.Among all Otsu’s methods, 3 D Otsu technique provides the best threshold values for the multi-level thresholding processes. In this paper, to improve the quality of segmented images, a simple and effective multilevel thresholding method is introduced. The proposed approach focuses on preserving edge detail by computing the 3 D Otsu along the fusion phenomena. The advantages of the presented scheme include higher quality outcomes, better preservation of tiny details and boundaries and reduced execution time with rising threshold levels. The fusion approach depends upon the differences between pixel intensity values within a small local space of an image;it aims to improve localized information after the thresholding process. The fusion of images based on local contrast can improve image segmentation performance by minimizing the loss of local contrast, loss of details and gray-level distributions. Results show that the proposed method yields more promising segmentation results when compared to conventional1 D Otsu, 2 D Otsu and 3 D Otsu methods, as evident from the objective and subjective evaluations. | Ashish Kumar Bhandari Arunangshu Ghosh Immadisetty Vinod Kumar | 2020 | IEEE/CAA Journal of Automatica Sinica2020，7，1： | 5 |
| 15 | Algal biomass as a global source of transport fuels:Overview and development perspectives显示文摘As a result of the global fuel crisis of the early 1970 s,coupled with concerns for the environment,the use of biofuel has been on the increase in many regions throughout the world.At present,a total of approximately 30 billion(30×109) liters of biofuel are utilized worldwide annually,although most countries rely hugely on the first generation biofuel.The limitations of the first and second generation biofuel gave rise to current interest in algae as a promising alternative to these conventional biofuel sources.Algal biomass could provide a lion's share of the global transport fuel requirements in future.The present review highlights some important developments in,and potentials of algaculture as a major biomass resource of the future.However,the major constraint to commercial-scale algae farming for energy production is the cost factor,which must be addressed adequately before its potentials can be harnessed. | Kifayat Ullah Mushtaq Ahmad Sofia Vinod Kumar Sharma Pengmei Lu Adam Harvey Muhammad Zafar Shazia Sultana C.N.Anyanwu | 2014 | Progress in Natural Science:Materials International2014，24，4： | 5 |
| 16 | Acute kidney injury and hepatorenal syndrome in cirrhosis显示文摘Acute kidney injury(AKI)in cirrhosis,including hepatorenal syndrome(HRS),is a common and serious complication in cirrhotic patients,leading to significant morbidity and mortality.AKI is separated into two categories,non-HRS AKI and HRS-AKI.The most recent definition and diagnostic criteria of AKI in cirrhosis and HRS have helped diagnose and prognosticate the disease.The pathophysiology behind non-HRS-AKI and HRS is more complicated than once theorized and involves more processes than just splanchnic vasodilation.The common biomarkers clinicians use to assess kidney injury have significant limitations in cirrhosis patients;novel biomarkers being studied have shown promise but require further studies in clinical settings and animal models.The overall management of non-HRS AKI and HRS-AKI requires a systematic approach.Although pharmacological treatments have shown mortality benefit,the ideal HRS treatment option is liver transplantation with or without simultaneous kidney transplantation.Further research is required to optimize pharmacologic and nonpharmacologic approaches to treatment.This article reviews the current guidelines and recommendations of AKI in cirrhosis. | Kapil Gupta Abhishek Bhurwal Cindy Law Scott Ventre Carlos D Minacapelli Savan Kabaria You Li Christopher Tait Carolyn Catalano Vinod K Rustgi | 2021 | World Journal of Gastroenterology2021，27，26： | 5 |
| 17 | Clinicopathological significance of synchronous carcinoma in colorectal cancer显示文摘 | Alfred King-Yin Lam Robert Carmichael Petra Gertraud Buettner Vinod Gopalan Yik-Hong Ho Simon Siu | 2011 | The American Journal of Surgery2011，，1： | 4 |
| 18 | Tailoring active compounds across biological membranes by cubosomal technology: an updated review显示文摘It is challenging for many drugs to be transported across various biological membranes. Furthermore, development of many drugs gets thwarted owing to their hydrophilic nature. The bioavailability of such drugs, which is the function of their ability to cross the membrane, tends to be low and exhibit high intra and inter subject variability. At present, formulation scientists are pursuing many projects for transdermal, nasal, target delivery of many active compounds, and it is prudent to explore alternative possibilities. Cubosomes offer transportation and tailoring of active compounds intended for both systemic and dermal delivery. Cubosomes are dispersed, self-assembled nanoparticles of bicontinuous cubic liquid crystalline phase formed from lipid and surfactant systems. Monoolein, poloxamer 407 and polyvinyl alcohol are the mostly used ingredients in the formulation of cubosomes. The adjustment in lipid composition can control the internal and structural changes of cubosomes. Based on the nodal surfaces, three structures of cubosomes proposed are Pn3m, Ia3d and Im3m. Top-down and bottom-up techniques are widely considered in the formulation process of extreme viscous bulk phase and aggregate from a precursor respectively. This article gives a bird's eye view about the engineering, characterization and evaluation of cubosomes, covering researches and applications of cubosomes done till date. | Vinod K.R. Sravya K. Sandhya S. David Banji Anbazhagan S. Prameela Rani A. | 2013 | Journal of Chinese Pharmaceutical Sciences2013，22，4： | 4 |
| 19 | Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial显示文摘 | Michael P Manns John G McHutchison Stuart C Gordon Vinod K Rustgi Mitchell Shiffman Robert Reindollar Zachary D Goodman Kenneth Koury Mei-Hsiu Ling Janice K Albrecht | 2001 | The Lancet2001，，9286： | 4 |
| 20 | Pseudomonas fluorescens-like bacteria from the stomach:A microbiological and molecular study显示文摘AIM:To characterize oxidase-and urease-producing bacterial isolates,grown aerobically,that originated from antral biopsies of patients suffering from acid peptic diseases.METHODS:A total of 258 antral biopsy specimens were subjected to isolation of bacteria followed by tests for oxidase and urease production,acid tolerance and aerobic growth.The selected isolates were further characterized by molecular techniques viz.amplifications for 16S rRNA using universal eubacterial and HSP60 gene specific primers.The amplicons were subjected to restriction analysis and partial sequencing.A phylogenetic tree was generated using unweighted pair group method with arithmetic mean(UPGMA) from evolutionary distance computed with bootstrap test of phylogeny.Assessment of acidity tolerance of bacteria isolated from antrum was performed using hydrochloric acid from 10-7 mol/L to 10-1 mol/L.RESULTS:Of the 258 antral biopsy specimens collected from patients,179(69.4%) were positive for urease production by rapid urease test and 31%(80/258) yielded typical Helicobacter pylori(H.pylori) after 5-7 d of incubation under a microaerophilic environment.A total of 240(93%) antral biopsies yielded homogeneous semi-translucent and small colonies after overnight incubation.The partial 16S rRNA sequences revealed that the isolates had 99% similarity with Pseudomonas species.A phylogenetic tree on the basis of 16S rRNA sequences denoted that JQ927226 and JQ927227 were likely to be related to Pseudomonas fluorescens(P.fluorescens).On the basis ofHSP60 sequences applied to the UPGMA phylogenetic tree,it was observed that isolated strains in an aerobic environment were likely to be P.fluorescens,and HSP60 sequences had more discriminatory potential rather than 16S rRNA sequences.Interestingly,this bacterium was acid tolerant for hours at low pH.Further,a total of 250(96.9%) genomic DNA samples of 258 biopsy specimens and DNA from 240 bacterial isolates were positive for the 613 bp amplicons by targeting P.fluorescens-specific conserved putative outer membrane protein gene sequences.CONCLUSION:This study indicates that bacterial isolates from antral biopsies grown aerobically were P.fluorescens,and thus acid-tolerant bacteria other than H.pylori can also colonize the stomach and may be implicated in pathogenesis/protection. | Saurabh Kumar Patel Chandra Bhan Pratap Ajay Kumar Verma Ashok Kumar Jain Vinod Kumar Dixit Gopal Nath | 2013 | World Journal of Gastroenterology2013，19，7： | 4 |
您所在的机构暂未开通此部分的功能，请联系管理员