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| 1 | Linagliptin alleviates fatty liver disease in diabetic db/db mice显示文摘AIM To study the effects of linagliptin on the structural signs of non-alcoholic fatty liver disease(NAFLD) in db/db mice. METHODS Male diabetic db /db mice(BKS.Cg-Dock7m+/+Leprdb/J) aged 10 wk received the dipeptidyl peptidase 4(DPP4) inhibitor linagliptin(10 mg/kg) or saline as a placebo once per day by gavage for 8 wk. Intact db/db mice served as controls. Structural changes in the liver were analyzed from light and electron microscopic images of sections from intact, placebo-treated and linagliptin-treated animals. We estimated the changes in hepatocytes, sinusoidal cells, liver microvasculature and lymphatic roots. Hepatic staining for lymphatic vessel endothelial hyaluronan receptor-1(LYVE-1) was assessed by immunohistochemistry. RESULTS In 18-wk-old diabetic mice, liver steatosis(predominantly microvesicular and mediovesicular steatosis) was accompanied by dilation of the roots of the lymphatic system, interlobular blood vessels and bile canaliculi. Compared to saline-treated mice, linagliptin-treated mice exhibited a reduction in the mean numeral densities of hepatocytes with lipid droplets(92.4% ± 1.7% vs 64.9% ± 5.8% per field of view, P = 0.0002) and a lower proportion of hepatocytes with a high density of lipid droplets(20.7% ± 3.6% vs 50.4% ± 3.1%, P = 0.0007). We observed heterogeneous hepatocytes and relatively preserved cell structures in the linagliptin group. Dilation of blood and lymphatic vessels, as well as ultrastructural changes in the hepatocyte endoplasmic reticulum and mitochondria, were alleviated by linagliptin treatment. In intact and placebo-treated mice, immunohistochemical staining for LYVE-1 was observed in the endothelial cells of interlobular lymphatic vessels and on the membranes of some endothelial sinusoidal cells. We observed an enlarged LYVE-1 reaction area in linagliptin-treated mice compared to intact and placebo-treated mice. The improvement in the structural parameters of the liver in linagliptin-treated mice was independent to changes in the plasma glucose levels. CONCLUSION The DPP4 inhibitor linagliptin alleviates liver steatosis and structural changes in the hepatic microvasculature and lymphatic roots in a model of NAFLD in diabetic db/db mice. | Svetlana V Michurina Irina Ju Ishenko Vadim V Klimontov Sergey A Archipov Natalia E Myakina Marina A Cherepanova Eugenii L Zavjalov Galina V Koncevaya Vladimir I Konenkov | 2016 | World Journal of Diabetes2016，7，19： | 2 |
| 2 | Isolation of antigens and antibodies by affinity chromatography 显示文摘 | Vladimir I Muronetz Timo K | 2003 | Chromatography B2003，790，12： | 2 |
| 3 | Color Doppler sonography and angioscintigraphy in hepatic Hodgkin's lymphoma显示文摘AIM:To estimate the characteristics of Color Doppler findings and the results of hepatic radionuclide angiography (HRA) in secondary Hodgkin's hepatic lymphoma.METHODS:The research included patients with a diagnosis of Hodgkin's lymphoma with metastatic focal lesions in the liver and controls.Morphologic characteristics of focal liver lesions and hemodynamic parameters were examined by pulsed and Color Doppler in the portal,hepatic and splenic veins were examined.Hepatic perfusion index (HPI) estimated by HRA was calculated.RESULTS:In the majority of patients,hepatomegaly was observed.Lesions were mostly hypoechoic and mixed,solitary or multiple.Some of the patients presented with dilated splenic veins and hepatofugal blood flow.A pulse wave was registered in the centre and at the margins of lymphoma.The average velocity of the pulse wave was higher at the margins (P>0.05).A continuous venous wave was found only at the margins of lymphoma.There was no linear correlation between lymphoma size and velocity of pulse and continuous wave (r=390,P<0.01).HPI was significantly lower in patients with lymphomas than in controls (P<0.05),pointing out increased arterial perfusion in comparison to portal perfusion.CONCLUSION:Color Doppler ultrasonography is a sensitive method for the detection of neovascularization in Hodgkin's hepatic lymphoma and estimation of its intensity.Hepatic radionuclide angiography can additionally help in the assesment of vascularisation of liver lesions. | Mirjana V Stojkovi Vera M Artiko Irena B Radoman Slavko J Knezevi Snezana M Luki Mirko D Kerkez Nebojsa S Leki Andrija A Anti Marinko M Zuvela Vitomir I Rankovi Milorad N Petrovi Dragana P obi Vladimir B Obradovi | 2009 | World Journal of Gastroenterology2009，15，26： | 2 |
| 4 | Postoperative change of anti-Thomsen-Friedenreich and Tn IgG level: The follow-up study of gastrointestinal cancer patients显示文摘AIM: To study the influence of tumor removal on the serum level of IgG antibodies to tumor-associated Thomsen-Friedenreich (TF), Tn carbohydrate epitopes and xenogeneic αGal, and to elucidate on the change of the level during the follow-up as well as its association with the stage and morphology of the tumor and the values of blood parameters in gastrointestinal cancer. METHODS: Sixty patients with gastric cancer and 34 patients with colorectal cancer in stages Ⅰ-Ⅳ without distant metastases were subjected to follow- up. The level of antibodies in serum was determined by the enzyme-linked immunosorbent assay (ELISA) using synthetic polyacrylamide (PAA) glycoconjugates. Biochemical and haematological analyses were performed using automated equipment. RESULTS: In gastrointestinal cancer, the TF antibody level was found to have elevated significantly after the removal of G3 tumors as compared with the preoperative level (u = 278.5, P < 0.05). After surgery, the TF and Tn antibody level was elevated in the majority of gastric cancer patients (sign test, 20 vs 8, P < 0.05, and 21 vs 8, P < 0.05, respectively). In gastrointestinal cancer, the elevated postoperative level of TF, Tn and αGal antibodies was noted in most patients with G3 tumors (sign test, 22 vs 5, P < 0.01; 19 vs 6, P < 0.05; 24 vs 8, P < 0.01, respectively), but the elevation was not significant in patients with G1 + G2 resected tumors. The postoperative follow-up showed that the percentage of patients with G3 resected tumors of the digestive tract, who had a mean level of anti-TF IgG above the cut- off value (1.53), was significantly higher than that of patients with G1 + G2 resected tumors (χ2 = 3.89, all patients; χ2 = 5.34, patients without regional lymph node metastases; P < 0.05). The percentage of patients with a tumor in stage I, whose mean anti-TF IgG level remained above the cut-off value (1.26), was significantly higher than that of patients with the cancer in stages Ⅲ-Ⅳ (χ2 = 4.71, gastric cancer; χ2 = 4.11, gastrointestinal cancer; P < 0.05). The correlation was observed to exist between the level of anti-TF IgG and the count of lymphocytes (r = 0.517, P < 0.01), as well as between the level of anti- Tn IgG and that of serum CA 19-9 (r = 0.481, P < 0.05). No positive delayed-type hypersensitivity reaction in skin test challenges with TF-PAA in any of the fifteen patients, including those with a high level of anti-TF IgG, was observed. CONCLUSION: The surgical operation raises the level of anti-carbohydrate IgG in most patients, especially in those with the G3 tumor of the gastrointestinal tract. The follow-up demonstrates that after surgery the low preoperative level of TF antibodies may be considerably increased in patients with the carcinoma in its early stage but remains low in its terminal stages. The stage- and morphology-dependent immunosuppression affects the TF-antibody response and may be one of the reasons for unresponsiveness to the immunization with TF-antigens. | Eugeniy P Smorodin Oleg A Kurtenkov Boris L Sergeyev Kristel E Kodar Valentin I Chuzmarov Vladimir P Afanasyev | 2008 | World Journal of Gastroenterology2008，14，27： | 2 |
| 5 | A branch and bound method for stochastic global optimization显示文摘 | Vladimir I Norkin Georg Ch Pfiug and Andrzej Ruszczynski | 1998 | Math Prog1998，83，： | 2 |
| 6 | Predictors of microsurgical varicocelectomy efficacy in male infertility treatment:critical assessment and systematization显示文摘In this review,we tried to systematize all the evidence(from PubMed[MEDLINE],Scopus,Cochrane Library,EBSCO,Embase,and Google Scholar)from 1993 to 2021 on the predictors of microsurgical varicocelectomy efficacy in male infertility treatment.Regarding the outcomes of varicocele repair,we considered semen improvement and pregnancy and analyzed them separately.Based on the 2011 Oxford CEBM Levels of Evidence,we assigned a score to each trial that studied the role of the predictor.We systematized the studied predictors based on the total points,which were,in turn,calculated based on the number and quality of studies that confirmed or rejected the studied predictor as significant,into three levels of significance:predictors of high,moderate,and low clinical significance.Preoperative total motile sperm count(TMSC)coupled with sperm concentration can be a significant predictor of semen improvement and pregnancy after varicocelectomy.In addition,for semen improvement alone,scrotal Doppler ultrasound(DUS)parameters,sperm DNA fragmentation index(DFI),and bilateral varicocelectomy are reliable predictors of microsurgical varicocelectomy efficacy. | Azizbek B Shomarufov Vladimir A Bozhedomov Nikolay I Sorokin Igor P Matyukhov Abdukodir A Fozilov Shukhrat A Abbosov Armais A Kamalov | 2023 | Asian Journal of Andrology2023，25，1： | 2 |
| 7 | Advances in small animal mesentery models for in vivo flow cytometry,dynamic microscopy,and drug screening显示文摘Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo. Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including in vivo label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cell’s functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo- and radio-sensitivity tests, and drug screening, are also discussed. | Ekaterina I Galanzha Valery V Tuchin Vladimir P Zharov | 2007 | World Journal of Gastroenterology2007，13，2： | 2 |
| 8 | Study of multiwalled graphite nanotubes and filaments formation from carbonized products of polyvinyl alcohol via catalytic graphitization at 600 ~800 ℃ in nitrogen atmosphere显示文摘 | KRIVORUCHKO Oleg P MAKSIMOVA Nadezhda I ZAIKOVSKII Vladimir I | 2000 | Carbon2000，38，7： | 1 |
| 9 | Theo- retical Analysis of Schemes for Complex Coal Process- ing显示文摘 | VLADIMIR I ANIKEEV ANDREY V | 1997 | Fuel Processing Technology1997，，： | 1 |
| 10 | Pre-activity mod- ulation of lower extremity muscles within different types and heights of deep jump显示文摘 | VLADIMIR M DUSKO B I NENAD J | 2008 | J Sports Sci Med2008，7，2： | 1 |
| 11 | Effects of operating conditions and fuel properties on emission performance and combustion efficiency of a swirling fluidized-bed combustor fired with a biomass fuel显示文摘 | VLADIMIR I KUPRIANOV RACHADAPORN KAEWKLUM | 2011 | Energy2011，36，： | 1 |
| 12 | Influence of ECAP routes on the microstructure and properties of pure Ti显示文摘 | Zhu Y T Alexandrov I V | 2001 | Materials Science and Engineering A2001，299，12： | 1 |
| 13 | Use of protein-protein interactions in affinity chromatography显示文摘 | Muronetz Vladimir I Sholukh Mikhail Korpela Timo | 2001 | J Biochem Biophys Methods2001，49，： | 1 |
| 14 | Photoacoustic flow cytom- etry显示文摘 | Ekaterina I G Vladimir P Z | 2012 | Methods2012，57，3： | 1 |
| 15 | Low-grade fibromyxoid sarcoma of the liver: A case report显示文摘BACKGROUND Low grade fibromyxoid sarcoma(LGFMS)is a rare and benign mesenchymal tumor with indolent course,most commonly found in young or middle-aged men.The majority of the LGFMSs are located in the trunk and deep soft tissue of the lower extremities.They appear as well circumscribed,although not encapsulated,which often leads to incomplete surgical resection.Despite their seemingly benign appearance,these tumors have aggressive behavior with high metastatic and recurrence rates.Accurate histopathologic examination of the specimen and its immunohistochemical analysis are mandatory for a precise diagnosis.CASE SUMMARY We report a case of a 38 year-old-man who presented with jaundice and upper abdominal discomfort.Multi-detector computed tomography and magnetic resonance imaging showed a large left liver tumor mass,extending to the hepatoduodenal ligament.Left hepatectomy was performed with resection and reconstruction of hepatic artery and preservation of middle hepatic vein.Histopathologic examination confirmed the tumor being a low-grade fibromyxoid sarcoma.Three and a half years after surgery,the patient died after being diagnosed with spine metastasis.CONCLUSION Due to poor response to all modalities of adjuvant treatment,we consider that the focus of treatment should be on surgery as the only option for curing the disease. | Vladimir Dugalic Igor I Ignjatovic Jelena Djokic Kovac Nikola Ilic Jelena Sopta Slavenko R Ostojic Dragan Vasin Marko D Bogdanovic Igor Dumic Tamara Milovanovic | 2021 | World Journal of Clinical Cases2021，9，1： | 1 |
| 16 | Gonservation of wild reideer in kamehaka显示文摘 | Vladimir I M | 2000 | Rangifer2000，，： | 1 |
| 17 | Isolation of antigens and antibodies by affinity chromato- graphy 显示文摘 | Vladimir I Muronetz Korpela T | 2003 | Journal of Chromatography B2003，790，： | 1 |
| 18 | 2-D Orthotropic failure criteria for masonry显示文摘 | Vadim I Lishak Vladimir I Yagust David ZYankelevsky | 2012 | Engineering Structures2012，36，36： | 1 |
| 19 | First- principles calculations of the electronic structure and spectra of strongly correlated systems: the LDA + U method显示文摘 | Vladimir I A Aryasetiawan F Lichtenstein A I | 1997 | Journal of Physics: Condensed Matter1997，9，4： | 1 |
| 20 | Regiospecific synthesis of α-alkoxy-α-sulfenylated ketones显示文摘 | Koumsch Abbaspour Tehrani Marc Boeykens Vladimir I Tyvorskii et a1 | 2000 | Tetrahedron2000，56，： | 1 |
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