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您的检索式：作者名="Vaughn L F"
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| 1 | Immunogenetic factors driving formation of ultralong VH CDR3 in Bos taurus antibodies显示文摘The antibody repertoire of Bos taurus is characterized by a subset of variable heavy(VH)chain regions with ultralong third complementarity determining regions(CDR3)which,compared to other species,can provide a potent response to challenging antigens like HIV env.These unusual CDR3 can range to over seventy highly diverse amino acids in length and form uniqueβ-ribbon‘stalk’and disulfide bonded‘knob’structures,far from the typical antigen binding site.The genetic components and processes for forming these unusual cattle antibody VH CDR3 are not well understood.Here we analyze sequences of Bos taurus antibody VH domains and find that the subset with ultralong CDR3 exclusively uses a single variable gene,IGHV1-7(VHBUL)rearranged to the longest diversity gene,IGHD8-2.An eight nucleotide duplication at the 3′end of IGHV1-7 encodes a longer V-region producing an extended Fβ-strand that contributes to the stalk in a rearranged CDR3.A low amino acid variability was observed in CDR1 and CDR2,suggesting that antigen binding for this subset most likely only depends on the CDR3.Importantly a novel,potentially AID mediated,deletional diversification mechanism of the B.taurus VH ultralong CDR3 knob was discovered,in which interior codons of the IGHD8-2 region are removed while maintaining integral structural components of the knob and descending strand of the stalk in place.These deletions serve to further diversify cysteine positions,and thus disulfide bonded loops.Hence,both germline and somatic genetic factors and processes appear to be involved in diversification of this structurally unusual cattle VH ultralong CDR3 repertoire. | Thaddeus C Deiss Melissa Vadnais Feng Wang Patricia L Chen Ali Torkamani Waithaka Mwangi Marie-Paule Lefranc Michael F Criscitiello Vaughn V Smider | 2019 | Cellular & Molecular Immunology2019，16，1： | 4 |
| 2 | Behavioral and physiological responses of Diaeretiella rapae to semiochemicals显示文摘 | VAUGHN T T ANTOLIN M F BJOSTAD L B | 1996 | Entomologia Experimentalis et Applicata1996，78，2： | 1 |
| 3 | Development of red pigmentation in Chlorococcum wimmeri ( Chlorophyta: Chlorococcales) 显示文摘 | Brown T E Richardson F L Vaughn M L | 1967 | Phycologia1967，6，： | 1 |
| 4 | Experimental reproduction of pneumonia in gnotobiotic pigs with porcine respiratory coronavirus isolate AR310显示文摘 | Halbur P Haridon R L Vaughn J F | 1993 | J Vet Diagn Invest1993，，5： | 1 |
| 5 | Use of the thick adherend shear test for shear stress-strain measurements of stiff and flexible adhesives显示文摘 | Kadioglu F Vaughn L F Guild F J | 2002 | The Journal of Adhesion2002，78，5： | 1 |
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