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| 1 | Effects of Saccharomyces cerevisiae fermentation products on performance and rumen fermentation and microbiota in dairy cows fed a diet containing low quality forage显示文摘Background: A possible option to meet the increased demand of forage for dairy industry is to use the agricultural byproducts, such as corn stover. However, nutritional value of crop residues is low and we have been seeking technologies to improve the value. A feeding trial was performed to evaluate the effects of four levels of Saccharomyces cerevisiae fermentation product(SCFP; Original XP; Diamond V) on lactation performance and rumen fermentation in mid-lactation Holstein dairy cows fed a diet containing low-quality forage. Eighty dairy cows were randomly assigned into one of four treatments: basal diet supplemented with 0, 60, 120, or 180 g/d of SCFP per head mixed with 180, 120, 60, or 0 g of corn meal, respectively. The experiment lasted for 10 wks, with the first 2 weeks for adaptation.Results: Dry matter intake was found to be similar(P > 0.05) among the treatments. There was an increasing trend in milk production(linear, P ≤ 0.10) with the increasing level of SCFP supplementation, with no effects on contents of milk components(P > 0.05). Supplementation of SCFP linearly increased(P < 0.05) the N conversion, without affecting rumen pH and ammonia-N(P > 0.05). Increasing level of SCFP linearly increased(P < 0.05) concentrations of ruminal total volatile fatty acids, acetate, propionate, and butyrate, with no difference in molar proportion of individual acids(P > 0.05). The population of fungi and certain cel ulolytic bacteria(Ruminococcus albus, R. flavefaciens and Fibrobacter succinogenes)increased linearly(P < 0.05) but those of lactate-utilizing(Selenomonas ruminantium and Megasphaera elsdeni) and lactate-producing bacteria(Streptococcus bovis) decreased linearly(P ≤ 0.01) with increasing level of SCFP. The urinary purine derivatives increased linearly(P < 0.05) in response to SCFP supplementation, indicating that SCFP supplementation may benefit for microbial protein synthesis in the rumen.Conclusions: The SCFP supplementation was effective in maintaining milk persistency of mid-lactation cows receiving diets containing low-quality forage. The beneficial effect of SCFP could be attributed to improved rumen function; 1)microbial population shift toward greater rumen fermentation efficiency indicated by higher rumen fungi and cel ulolytic bacteria and lower lactate producing bacteria, and 2) rumen microbial fermentation toward greater supply of energy and protein indicated by greater ruminal VFA concentration and increased N conversion. Effects of SCFP were dose-depended and greater effects being observed with higher levels of supplementation and the effect was more noticeable during the high THI environment. | Wen Zhu Zihai Wei Ningning Xu Fan Yang Ilkyu Yoon Yihua Chung Jianxin Liu Jiakun Wang | 2017 | Journal of Animal Science and Biotechnology2017，8，3： | 13 |
| 2 | Cultivation of microalgae for biodiesel production:A review on upstream and downstream processing显示文摘Fluctuating market price of fossil fuel and overwhelming emission of greenhouse gases to the atmosphere have resulted in climate change and have been a global concern in this decade. Hence, biodiesel has become an alternative option to fossil diesel as it is renewable and environmentally friendly. Nevertheless, this alternative fuel that is usually derived from terrestrial oil crops will cause shortage in food supply and deforestation if mass production is realized. In recent years, cultivation of aquatic microorganism(particularly microalgae) to produce biodiesel is considered as a practical solution due to their high growth rate and ability to synthesize large quantity of lipid within their cell. However, the development of energy and cost-efficiency of microalgae cultivation system are the main issues in producing renewable microalgae biodiesel. Of late, wastewater or organic compost has been used as the cultivation medium as it can provide sufficient nutrients to sustain microalgae growth.Microalgae cultivation method and system are vitally important as these factors undoubtedly affect the final microalgae biomass and lipid yield. In this review, the cultivation system of microalgae, nutrients demanded for microalgae production, cell harvesting and drying, microalgae oil extraction, and utilization of microalgae biomass for biodiesel production are introduced and discussed. It is anticipated to convey clearer perspectives in upstream and downstream processes in microalgae-derived biodiesel production. | Xin Bei Tan Man Kee Lam Yoshimitsu Uemura Jun Wei Lim Chung Yiin Wong Keat Teong Lee | 2018 | Chinese Journal of Chemical Engineering2018，26，1： | 6 |
| 3 | Induction of HLA-G expression in a melanoma cell line OCM-1A following the treatment with 5-aza-2'-deoxycytidine显示文摘The non-classical HLA class I antigen HLA-G is an immune modulator which inhibits the functions of T cells, NK cells, and the Dendritic cells (DC). As a result, HLA-G expression in malignant cells may provide them with a mechanism to escape the immune surveillance. In melanoma, HLA-G antigen expression has been found in 30% of surgically removed lesions but in less than 1% of established cell lines. One possible mechanism underlying the differential HLA-G expression in vivo and in vitro is that the HLA-G gene is epigenetically repressed in melanoma cells in vitro. To test this hypothesis, we treated the HLA-G negative melanoma cell line OCM-1A with the DNA methyltransferase inhibitor 5-aza-2’-deoXycytidine (5-AC) and analyzed whether HLA-G expression can be restored. Our data strongly suggest that HLA-G is silenced as a result of CpG hypermethylation within a 5’ regulatory region encompassing 220 bp upstream of the start codon. After treatment, HLA-G mRNA expression was dramatically increased. Western blot and flow cytometry showed that HLA-G protein was induced. Interestingly, HLA-G cell surface expression on the 5-AC treated OCM-1 A cells is much less than that on the HLA-G positive JEG-3 cells while a similar amount of total HLA-G was observed. Possible mechanisms for the difference were analyzed in the study such as cell cold-treatment, peptide loading and antigen processing machinery components (APM) as well as β2 microglobulin (β2-m) expression. Data revealed that the APM component calreticulin might be involved in the lower HLA-G surface expression on OCM-1 A cells. Taken together, our results indicated that DNA methylation is an important epigenetic mechanism by which HLA-G antigen expression is modulated in melanoma cells in vitro. Furthermore, to the first time, we hypothesized that the deficiency of calreticulin might be involved in the low HLA-G surface expression on the 5-AC treated OCM-1 A cells. | Wei Hua YAN Ai Fen LIN Chien Chung CHANG Soldano FERRONE | 2005 | Cell Research2005，15，7： | 5 |
| 4 | Tree of life for the genera of Chinese vascular plants显示文摘我们重建了使用的中国脉管的植物(Tracheophyta ) 的一棵种系发生的树叶绿体基因 atpB, matK, ndhF,和 rbcL 和 mitochondrial matR 定序。我们生产了包括 6098 种类并且包括 13 个 695 DNA 序列, 1803 最新被产生的一个矩阵。我们的分类采样跨越了代表中国脉管的植物的 323 个家庭的 3114 个类,盖住从中国知道的超过 93% 所有类。全面大发展史支持大多数关系在之中并且在最近的分子的种系发生的研究为 lycophytes,蕨纲植物(monilophytes ) ,裸子植物,和被子植物认出的家庭以内。为被子植物,在被子植物发展史组 IV 的大多数家庭作为 monophyletic 被养活,除了 paraphyletic Dipterocarpaceae 和 Santalaceae。一些大类的几个大家庭并且 monophyly 的 infrafamilial 关系被我们的稠密的分类采样很好支持。我们的结果证明 Eberhardtia 的二种是到 Sapotaceae 的所有另外的 taxa 形成的 clade 的姐妹,除了 Sarcosperma。我们经由 SoTree (http://gffzz52bf878016c04d01hk9ofcxo0nxu06kuq.ffgz.tsg.suse.edu.cn/flora/index.shtml ) 做了我们为子树的创造公开地可得到的中国脉管的植物的发展史,为生态学家的一个自动化发展史集会工具。 | Zhi-Duan Chen Tuo Yang Li Lin Li-Min Lu Hong-Lei Li Miao Sun Bing Liu Min Chen Yan-Ting Niu Jian-Fei Ye Zhi-Yong Cao Hong-Mei Liu Xiao-Ming Wang Wei Wang Jing-Bo Zhang Zhen Meng Wei Cao Jian-Hui Li Sheng-Dan Wu Hui-Ling Zhao Zhong-Jian Liu Zhi-Yuan Du Qing-Feng Wang Jing Guo Xin-Xin Tan Jun-Xia Su Lin-Jing Zhang Lei-Lei Yang Yi-Ying Liao Ming-He Li Guo-Qiang Zhang Shih-Wen Chung Jian Zhang Kun-Li Xiang Rui-qi Li Douglas E. Soltis Pamela S. Soltis Shi-Liang Zhou Jin-Hua Ran Xiao-quan Wang Xiao-Hua Jin You-Sheng Chen Tian-Gang Gao Jian-Hua Li Shou-Zhou Zhang An-Ming Lu China Phylogeny Consortium | 2016 | Journal of Systematics and Evolution2016，54，4： | 5 |
| 5 | Preliminary nitrite, nitrate and colour analysis of Malaysian edible bird’s nest显示文摘The high nitrite content in edible bird’s nests is a major concern to the local swiftlet industry.It lowers the price of the edible bird’s nests and it brings severe health hazards to consumers and farmers.This research investigated the nitrite and nitrate contents of eight types of local edible bird’s nests by using ion chromatography system and evaluating its colour using the CIE system in L^*a^*b^*parameters.The nitrite content obtained ranged from 5.7 lg/g for the house nests to 843.8 lg/g for the cave nests.The nitrate content for the house and cave nests was 98.2 lg/g and 36,999.4 lg/g,respectively.The cave nests with darker and redder colour had higher nitrite and nitrate contents than the brighter and more yellow house nests.This likely suggests that the nitrite and nitrate contents have correlations with edible bird’s nests colour.Correlations studies suggested that the nitrite content had high correlations with colour parameters,L^*a^*b^*of edible bird’s nests at significant level of P<0.10.These findings suggest that edible bird’s nests’colour may be a useful indicator for measuring nitrite and nitrate contaminations. | Meei Chien Quek Nyuk Ling Chin Yus Aniza Yusof Sheau Wei Tan Chung Lim Law | 2015 | Information Processing in Agriculture2015，2，1： | 3 |
| 6 | Downregulation of microRNA‐15b by hepatitis B virus X enhances hepatocellular carcinoma proliferation via fucosyltransferase 2‐induced Globo H expression显示文摘 | Chen‐Shiou Wu Chia‐Jui Yen Ruey‐Hwang Chou Jia‐Ni Chen Wei‐Chien Huang Chung‐Yi Wu Yung‐Luen Yu | 2014 | Int J Cancer2014，，7： | 3 |
| 7 | Interleukin-6 Is Required for Pancreatic Cancer Progression by Promoting MAPK Signaling Activation and Oxidative Stress Resistance显示文摘 | Yaqing Zhang Wei Yan Meredith A. Collins Filip Bednar Sabita Rakshit Bruce R. Zetter Ben Z. Stanger Ivy Chung Andrew D. Rhim Marina Pasca di Magliano | 2013 | Cancer Research2013，，20： | 2 |
| 8 | Advanced Noncarbon Materials as Catalyst Supports and Non‑noble Electrocatalysts for Fuel Cells and Metal–Air Batteries显示文摘Electrochemical energy systems such as fuel cells and metal–air batteries can be used as clean power sources in the field of electric transportation and possess great potential in the reduction of various energy and environmental issues.In these systems,the oxygen reduction reaction(ORR)at the cathode is the rate-determining factor for overall system performance,and up to now,platinum group metals supported on carbon materials,especially Pt,remain the highest performing and the most practical ORR electrocatalysts.However,corresponding carbonaceous catalyst supports are extremely susceptible to corrosion under electrochemical operation,and therefore,the extensive exploration of alternative stable materials for ORR electrocatalysts with both high electrochemical stability and catalytic performance is essential.Here,noncarbon materials with high corrosion resistance have been explored to substitute traditional carbon supports or even act directly as low-cost non-noble metal electrocatalysts,and based on this,this review will present a comprehensive overview and deep analysis of the recent progress in noncarbon materials,including metals,oxides,nitrides,carbides,sulfides,and so on.Overall,general attributes associated with noncarbon materials include high corrosion resistance,strong metal–support interaction,and impressive porous structure retention.However,major drawbacks include low electrical conductivity,insufficient chemical stability in acidic or alkaline media,and poor electrochemical stability at ORR electrode potentials.To overcome these challenges,this review will also summarize efficient strategies such as combining with highly conductive materials,introducing dopants and forming vacancies to result in promising electrocatalytic ORR performances.Finally,this review will propose possible research directions to facilitate future research and development toward the practical application of noncarbon-based ORR electrocatalysts. | Shiming Zhang Menghui Chen Xiao Zhao Jialin Cai Wei Yan Joey Chung Yen Shengli Chen Yan Yu Jiujun Zhang | 2021 | Electrochemical Energy Reviews2021，4，2： | 2 |
| 9 | Bile duct anastomotic stricture after adult‐to‐adult right lobe living donor liver transplantation显示文摘 | Kenneth Siu Ho Chok See Ching Chan Tan To Cheung William Wei Sharr Albert Chi Yan Chan Chung Mau Lo Sheung Tat Fan | 2011 | Liver Transpl2011，，1： | 2 |
| 10 | Paradoxical Increase in Ventricular Torsion and Systolic Torsion Rate in Type I Diabetic Patients Under Tight Glycemic Control显示文摘 | Jina Chung Paul Abraszewski Xin Yu Wei Liu Andrew J. Krainik Marvin Ashford Shelton D. Caruthers Janet B. McGill Samuel A. Wickline | 2006 | Journal of the American College of Cardiology2006，，2： | 2 |
| 11 | Efficacy of transient elastography in screening for large esophageal varices in patients with suspicious or proven liver cirrhosis显示文摘 | Huay‐Min WANG Gin‐Ho LO Wen‐Chi CHEN Ping‐I HSU Hsien‐Chung YU Chiun‐Ku LIN Hoi‐Hung CHAN Wei‐Lun TSAI Feng‐Woei TSAY Tsan‐Ping LIU E‐Ming WANG Kwok‐Hung LAI | 2012 | Journal of Digestive Diseases2012，，8： | 2 |
| 12 | The Efficacy of Second‐Line Anti‐ Helicobacter pylori Therapy Using an Extended 14‐Day Levofloxacin/Amoxicillin/Proton‐Pump Inhibitor Treatment – A Pilot Study显示文摘 | Seng‐Kee Chuah Wei‐Chen Tai Pin‐I Hsu Deng‐Chyang Wu Keng‐Liang Wu Chung‐Mou Kuo Yi‐Chun Chiu Ming‐Luen Hu Yeh‐Pin Chou Yuan‐Hung Kuo Chih‐Ming Liang King‐Wah Chiu Tsung‐Hui Hu | 2012 | Helicobacter2012，，5： | 2 |
| 13 | Sesmic Response Analysis of Bridges Isolated with Friction Pendulum Bearings显示文摘 | Wang Yen-Po Chung Lap-Loi hiao Wei Hsin | 1998 | Earthquake Engineering & Structural Dynamics1998，27，10： | 1 |
| 14 | Exploiting Sequence and Structure Homologs to Identify Protein-protein Binding Sites显示文摘 | Chung Jolan Wang Wei Bourne P E | 2006 | Proteins2006，62，3： | 1 |
| 15 | An outcome study of thumb reconstruction using microvascular toe transfer显示文摘 | Chung KC Wei FC | 2000 | Hand surg[Am]2000，25，4： | 1 |
| 16 | Are tannase a double-edged sword in biology and health 显示文摘 | King-Thom Chung Cheng- I Wei Michael G Johnson | 1998 | Trends in Food Science & Technology1998，，9： | 1 |
| 17 | Two RFID-based solutions for secure in patient medication administration显示文摘 | Yi Chung Yen Nai Wei Lo Tzong Chen Wu | | 0，，： | 1 |
| 18 | On SVD- based watermarking algorithm 显示文摘 | Kuo Liang Chung Wei Ning Yang Yong Huai I-Iuang | 2007 | Applied Mathematics and Comutation2007，155，： | 1 |
| 19 | Induction of humoral immune response and protective immuneity in chickens against salmonella enteritisdis after a single dose of killed bacterium-loaded microspheres 显示文摘 | Wei Liu Yiyan Yang Neal Chung | 2001 | Avian Dis2001，45，： | 1 |
| 20 | An outcome study of thumb reconstruction using microvascular toe transfer显示文摘 | Chung KC Wei FC | 2000 | J Hand Surg (Am)2000，25，4： | 1 |
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