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| 1 | Anticancer effect and apoptosis induction of gambogic acid in human gastric cancer line BGC-823显示文摘AIM: To investigate the anticancer effect of a traditional Chinese medicine gambogic acid (GA) in human gastric cancer line BGC-823 and further study the mechanism of apoptosis induction of GA.METHODS: Low differential human gastric cancer line BGC-823 were treated with GA at different doses and different times, the inhibitory rates were detected by MTT assay. Apoptosis induced by GA in BGC-823 cells was observed by Annexin-V/PI doubling staining flow cytometry assay. And T/C (%) was chosen to detect the inhibition of GA on human gastric adenocarcinoma BGC-823 nude mice xenografts. Apoptosis on nude mice xenografts was observed by Annexin-V/PI doubling staining flow cytometry assay and DNA fragmentation assay. To further determine the molecular mechanism of apoptosis induced by GA, the changes on the expression of bcl-2 and bax genes were detected by RT-PCR.RESULTS: After incubation with GA, low differential human gastric cancer line BGC-823 was dramatically inhibited in a dose-dependent manner. After these cells were exposedto GA for 24, 48 and 72 h, the IC50 value was 1.02±0.05, 1.41±0.20 and 1.14±0.19 μmol/L, respectively. Apoptosis in BGC-823 cells induced by GA was observed by AnnexinV/PI doubling staining flow cytometry assay. The apoptotic population of BGC-823 cells was about 12.96% and 24.58%, respectively, when cells were incubated with 1.2 μmol/L GA for 48 and 72 h. T/C (%) of human gastric carcinoma adenocarcinoma BGC-823 nude mice xenografts was 44.3, when the nude mice were treated with GA (8 mg/kg). Meanwhile, apoptosis induced by GA was observed in human gastric carcinoma adenocarcinoma BGC-823 nude mice xenografts. The increase of bax gene and the decrease of bc1-2 gene expressions were found by RT-PCR.CONCLUSION: The inhibition of GA on human gastric cancer line BGC-823 was confirmed. This effect connects with the inducing apoptosis in BGC-823 cells and the molecular mechanism might be related to the reduction of expression of apoptosis-regulated gene bcl-2, and the improvement of the expression of apoptosis-regulated gene bax. The result was also confirmed in vivo. | WeiLiu Qing-LongGuo Qi-DongYou LiZhao Hong-YanGu Sheng-TaoYuan | 2005 | World Journal of Gastroenterology2005，11，24： | 33 |
| 2 | Symptom-based tendencies of Helicobacter pylori eradication in patients with functional dyspepsia显示文摘AIM: To investigate whether there were symptom-based tendencies in the Helicobacter pylori (H. pylori) eradication in functional dyspepsia (FD) patients. METHODS: A randomized, single-blind, placebo-controlled study of H. pylori eradication for FD was conduct- ed. A total of 195 FD patients with H. pylori infection were divided into two groups: 98 patients in the treatment group were treated with rabeprazole 10 mg twice daily for 2 wk, amoxicillin 1.0 g and clarithromycin 0.5 g twice daily for 1 wk; 97 patients in the placebo group were given placebos as control. Symptoms of FD, such as postprandial fullness, early satiety, nausea, belching,epigastric pain and epigastric burning, were assessed 3 mo after H. pylori eradication. RESULTS: By per-protocol analysis in patients with successful H. pylori eradication, higher effective rates of 77.2% and 82% were achieved in the patients with epigastric pain and epigastric burning than those in the placebo group (P < 0.05). The effective rates for postprandial fullness, early satiety, nausea and belching were 46%, 36%, 52.5% and 33.3%, respectively, and there was no significant difference from the placebo group (39.3%, 27.1%, 39.1% and 31.4%) (P > 0.05). In 84 patients who received H. pylori eradication therapy, the effective rates for epigastric pain (73.8%) and epigastric burning (80.7%) were higher than those in the placebo group (P < 0.05). The effective rates for postprandial fullness, early satiety, nausea and belch- ing were 41.4%, 33.3%, 50% and 31.4%, respective- ly, and did not differ from those in the placebo group (P > 0.05). By intention-to-treat analysis, patients with epigastric pain and epigastric burning in the treatment group achieved higher effective rates of 60.8% and 65.7% than the placebo group (33.3% and 31.8%) (P < 0.05). The effective rates for postprandial fullness, early satiety, nausea and belching were 34.8%, 27.9%, 41.1% and 26.7% respectively in the treatment group, with no significant difference from those in the placebo group (34.8%, 23.9%, 35.3% and 27.1%) (P > 0.05). CONCLUSION: The efficacy of H. pylori eradication has symptom-based tendencies in FD patients. It may be effective in the subgroup of FD patients with epigastric pain syndrome. | LingLan ]ingYu Yu—LongChen Ya—LiZhong HaoZhang Chang HeJia YuanYuan Bo—WeiLiu | 2011 | World Journal of Gastroenterology2011，17，27： | 33 |
| 3 | Tissue engineering of blood vessels with endothelial cells differentiated from mouse embryonic stem cells显示文摘Endothelial cells (TEC3 cells) derived from mouse embryonic stem (ES) cells were used as seed cells to construct blood vessels. Tissue engineered blood vessels were made by seeding 8 × l06 smooth muscle cells (SMCs) obtained from rabbit arteries onto a sheet of nonwoven polyglycolic acid (PGA) fibers, which was used as a biodegradable polymer scaffold. After being cultured in DMEM medium for 7 days in vitro, SMCs grew well on the PGA fibers, and the cell-PGA sheet was then wrapped around a silicon tube, and implanted subcutaneously into nude mice. After 6~8 weeks, the silicon tube was replaced with another silicon tube in smaller diameter, and then the TEC3 cells (endothelial cells differentiated from mouse ES cells) were injected inside the engineered vessel tube as the test group. In the control group only culture medium was injected. Five days later, the engineered vessels were harvested for gross observation, histological and immunohistochemical analysis. The preliminary results demonstrated that the SMC-PGA construct could form a tubular structure in 6~8 weeks and PGA fibers were completely degraded. Histological and immunohistochemical analysis of the newly formed tissue revealed a typical blood vessel structure, including a lining of endothelial cells (ECs) on the lumimal surface and the presence of SMC and collagen in the wall. No EC lining was found in the tubes of control group. Therefore, the ECs differentiated from mouse ES cells can serve as seed cells for endothelium lining in tissue engineered blood vessels. | GANSHEN HSIAOCHIENTSUNG CHUNFANGWU XIAOYUNWANG WEILIU LEICUI YILINCAO | 2003 | Cell Research2003，13，5： | 22 |
| 4 | Enhanced visible-light photocatalytic activity of a g-C_3N_4/m-LaVO_4 heterojunction: band offset determination显示文摘Band offset is a dominant factor affecting the photocatalytic performance of heterostructure photocatalysts. Therefore, controlling the band gap structure of semiconductors is a key challenge in the development of efficient photocatalysts. We used a typical in situ-method to prepare diverse graphite-phase carbon nitride(g-C_3N_4)samples from melamine, thiourea, and a mixture thereof,and found that they exhibited band gaps between2.3–2.8 e V. From UV–Vis spectra and X-ray photoelectron spectroscopy measurements, we determined that the g-C_3N_4 samples exhibited different band gap values and valence band positions. On this basis, we constructed g-C_3N_4/m-La VO_4 heterojunctions with different band offsets. UV–Vis spectra and X-ray photoelectron spectroscopy measurements revealed that the valence band offsets(VBOs) of the different heterojunctions were similar, but their conduction band offsets(CBOs) were significantly different. Photocatalytic experiments revealed that the reaction rate was enhanced with an increase in the CBO value. Furthermore, the three-phase g-C_3N_4/g-C_3N_4/mLa VO_4 heterojunction composed of m-La VO_4 and mixed g-C_3N_4 showed the highest photocatalytic activity, which was mainly due to the construction of a multilevel structure. This work investigates the influence of the band offset on heterojunction photoelectrochemical properties and provides a new strategy to improve the photocatalytic activity by constructing multilevel structures. | Xiaoping Liu Hao Qin Weiliu Fan | 2016 | Science Bulletin2016，61，8： | 6 |
| 5 | Lipopolysaccharide Could Be Internalized into Human Peripheral Blood Mononuclear Cells and Elicits TNF-α Release,but not via the Pathway of Toll-Like Receptor 4 on the Cell Surface显示文摘Lipopolysaccharide (LPS [endotoxin]), the principal component of the outer membrane of gram-negative bacteria, stimulate various cell types to release numerous proinflammatory mediators such as TNF-α, IL-6 and IL-12, which may damage cells and lead to organ injury, even sepsis and septic shock. Toll-like receptor 4(TLR4) has been identified as the receptor involved in the recognition of LPS, but the role of LPS uptake in activating signal transduction remains controversial. In the present study, TNF-α was used as a marker of macrophages/monocytes activated by LPS, and CQ was used as an inhibitor of endosome mature in order to definitude what stage the signal transduction elicited by LPS was interrupted. We found that there indeed existed internalization of LPS and internalization partially participated in LPS signaling since CQ inhibited cytokine release, and decreased accumulation of FITC-LPS in hPBMC. In contrast, anti-hTLR4 antibody could decrease cytokines' release, but no inhibition on accumulation of FITC-LPS. This result revealed that inhibition of cytokine release was related to reduction of FITC-LPS accumulation in the cells. But TLR4 on the cell surface didn't possibly participated in internalization of LPS. Thus, LPS signaling and internalization cannot be viewed as mutually independent processes. Cellular & Molecular Immunology. 2004;1(5):373-377. | HongZhou GuofuDing WeiLiu LiangxiWang YonglingLu HongweiCao JiangZheng | 2004 | Cellular & Molecular Immunology2004，1，5： | 4 |
| 6 | COMBINED SURGICAL THERAPY FOR HEPATOLITHIASIS显示文摘Objective To evaluate the combined surgical therapy for calculi of intrahepatic duct. Methods One hundred and eight cases of hepatolithiasis treated in our hospital from January 1986 to September 2003 were summarized and analyzed retrospectively. The primary surgery included 57 cases of biliary tract exploration and cholangiolithotomy, 26 cases of cholangiojejunostomy, and 25 cases of partial hepatectomy. Of these cases, 156 operations were performed totally. There were 36 patients undergoing two or more operations for cholangiolithiasis problem. Fiberoptic choledochoscopy was employed to examine and remove the residual stones during and after operation in all cases. Results The residual stone rates were 57.89% (33/57), 26.92% (7/26), and 24.0% (6/25) for biliary tract exploration and cholangiolithotomy, cholangiojejunostomy, and partial hepatectomy, respectively. Fiberoptic choledochoscopy was utilized pre- and post-operatively for each patient, which was performed 2 to 9 times on each case. Following the combined treatment, the rate of residual stone after operation decreased to 12.96% (14/108). Conclusions The combined surgical therapy is valuable for resolving the problem of hepatolithiasis in most of the patients. Regarding the clinical outcomes of different surgical procedures, partial hepatectomy is superior to cholangiojejuno- stomy or biliary tract exploration and cholangiolithotomy. Fiberoptic choledochoscopy is also important to reduce the occurrence of residual stones and the rate of reoperation. | Xiao-dongHe WeiLiu Bing-luLi Zheng-huanZhang Jian-xiZhang | 2005 | Chinese Medical Sciences Journal2005，20，2： | 3 |
| 7 | Multiple gene differential expression patterns in human ischemic liver:Safe limit of warm ischemic time显示文摘AIM: To investigate the multiple gene differential expression pattems in human ischemic liver and to produce the evidence about the hepatic ischemic safety time.METHODS: The responses of cells to hepatic ischemia and hypoxia at hepatic ischemia were analyzed by cDNA microarrary representing 4 000 different human genes containing 200 apoptotic correlative genes. RESULTS: There were lower or normal expression levels of apoptotic correlative genes during the periods of hepatic ischemia for 0-15 min, the maintenance homostatic genes were expressed significantly higher at the same time. But at the hepatic ischemia for 30 min, the expression levels of maintenance homeostatic genes were down-regulated, the expressions of many apoptotic correlative genes and nuclear transcription factors were activated and up-regulated.CONCLUSION: HIF-1, APAF-1, PCDC10, FBX5, DFF-40, DFFA XIAP, survivin may be regarded as the signal genes to judge the degree of hepatic ischemic-hypoxic injure, and the apoptotic liver cell injury due to ischemia in different time limits. The safe limit of human hepatic warm ischemic time appears to be generally less then 30 min. | Qi-PingLu Ting-]iaCao Zhi-YongZhang WeiLiu | 2004 | World Journal of Gastroenterology2004，10，14： | 2 |
| 8 | Effects of Application of Cassava Alcohol Anaerobic Fermentation Liquid and Mulching of Film on Overwintering and Next Year's Growth and Yield of Banana显示文摘The effects of application of cassava alcohol anaerobic fermentation liquid and mulching of film on overwintering and next year's growth and yield of banana were investigated in this study.The results showed that the application of cassava alcohol anaerobic fermentation liquid with COD concentration of 1 200-2 000 mg/L(900 m^3/hm^2) and mulching of film significantly increased the soil organic matter and available potassium contents in banana orchard,improved the cold resistance of banana seedlings,increased the pseudostem height,pseudostem circumference,green leaf number and chlorophyll content of next year's banana seedlings,brought the flower bud emergence period and harvest period forward,and significantly increased the yield per plant. | Yunchuan MO Yingyi DENG Xu ZHENG Yixin YE Ganhui MO Minzheng WEI Weiliu LI Feng LI Jiechun PAN Chanchan QIN Meiling SU Yao FU | 2016 | Agricultural Science & Technology2016，17，7： | 2 |
| 9 | Comparative study of clinical Manifestation,plain-film radiography,and computed tomographic scan in arteriovenous malformations of the jaws显示文摘 | Xindong Fan Weiliu Qiu Zhiyuan Zhang | 2002 | Oral Surg Oral Med Oral Pathol Oral Radio Endod2002，94，4： | 1 |
| 10 | One-Step Preparation of Single-Crystalline β-MnO2 Nanotubes显示文摘 | Deshan Zheng Sixiu Sun Weiliu Fan | 2005 | J Phys Chem2005，109，16： | 1 |
| 11 | Preparation of a hydroxypropyl Ganoderma lucidum polysaccharide and its physicochemical properties显示文摘 | WeiLiu JingXu | 2010 | food chemistry2010，，122： | 1 |
| 12 | Hydrothermal formation and characterization of magnesium hydroxide chloride hydrate nanowires 显示文摘 | Fan Weiliu Song Xinyu Sun Sixiu | 2007 | Journal of Crystal Growth2007，305，1： | 1 |
| 13 | Prokaryotic expression and purification of Camellia sinensis polyphenol oxidase显示文摘 | Jing‐weiLiu You‐yiHuang JianDing CongLiu Xiu‐danXiao De‐jiangNi | 2010 | J Sci Food Agric2010，，14： | 1 |
| 14 | Synthesis ofhigh-quality monolayer and bilayer grapheme on copperusing chemical vapor deposition 显示文摘 | WeiLiu Hong Li Chuan Xu | 2011 | Carbon2011，49，2011： | 1 |
| 15 | Risk factors for intrahepatic and extrahepatic cholangiocarcinoma: a case–control study in China显示文摘 | Lian‐YuanTao Xiao‐DongHe QiangQu LeiCai WeiLiu LiZhou Shang‐MingZhang | 2009 | Liver International2009，，2： | 1 |
| 16 | Preparation of different morphologies of calcium sulfate in organic media显示文摘 | Song Xinyu Sun Sixiu Fan Weiliu | 2003 | Journal of Materials Chemistry2003，13，： | 1 |
| 17 | Solvothermal synthesis of Mg ( OH)2 nanotubes using Mg10 (OH) 18 C12 · 5H2O nanowires as precursors 显示文摘 | Fan Weiliu Sun Sixiu You Liping | 2003 | Journal of Materials Chemistry2003，13，12： | 1 |
| 18 | Is it pay levels or pay raises that matter to fairness and turnover?显示文摘 | Amanuel G.Tekleab Kathryn M.Bartol WeiLiu | 2005 | Behav2005，，8： | 1 |
| 19 | One-step preparation of single-crystalline β-MnO2 nanotuhes 显示文摘 | Zheng Deshan Sun Sixiu Fan Weiliu | 2005 | Physical Chemistry B2005，109，16： | 1 |
| 20 | Association of HLA-DQ with Idiopathic Dilated Cardiomyopathy in a Northern Chinese Han Population显示文摘Autoimmune mechanisms are likely involved in the pathogenesis of idiopathic dilated cardiomyopathy (IDC) and components of MHC may serve as markers for the propensity to develop immune-mediated myocardial damage. This study was conducted to investigate the possible association between HLA-DQA1, -DQB1 alleles and IDC in Han population from northern China by using PCR-based sequence-specific primer (PCR-SSP) technique for HLA genotyping. Among 68 unrelated IDC patients, 4 probands of IDC pedigrees and 100 healthy controls, we found that the alleles of HLA-DQA1*0501 and HLA-DQB1*0303 conferred susceptibility to IDC while HLA-DQA1*0201 and HLA-DQB1*0502, *0504 alleles were in negative association with IDC. The serine at position 57 (SER57) in the exon of HLA-DQB1*0502 and *0504 was confirmed in our experiment as a marker for resistance to IDC. The results suggest that HLA-DQ polymorphism may be involved in the pathogenesis of IDC. | WeiLiu WeiminLi NinglingSun | 2004 | Cellular & Molecular Immunology2004，1，4： | 1 |
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