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| 1 | Bulking sludge for PHA production:Energy saving and comparative storage capacity with well-settled sludge显示文摘Two acetate-fed sequencing batch reactors(SBR) were operated under an aerobic dynamic feeding(ADF) model(SBR #2) and with anaerobic phase before aerobic phase(SBR #1) to select mixed cultures with a high polyhydroxyalkanoates(PHA) storage response.Although kinetic selection based on storage response should bring about a predominance of floc-formers,a bulking sludge with storage response comparable to well-settled sludge was steadily established.An anaerobic phase was introduced before the aerobic phase in the ADF model to improve the sludge settleability(SBR #1),however,due to the consequent increased feast/famine ratio,the performance of SBR #1,in terms of both the maximum PHB(polyhydroxybutyrate) cell content and PHB,was lower than that of SBR #2.SBR #2 gradually reached a steady state while SBR #1 failed suddenly after 50 days of operation.The maximum specific substrate uptake rate and storage rate for the selected bulking sludge were 0.4 Cmol Ac/(Cmol X·hr) and 0.18 Cmol Ac/(Cmol PHB·hr),respectively,resulting a yield of 0.45 Cmol PHB/(Cmol Ac) in SBR #2 in the culture enrichment phase.A maximum PHB content of 53% of total suspended solids and PHB storage rate of 1.36 Cmol Ac/(Cmol PHB·hr) was achieved at 10.2 hr in batch accumulation tests under nitrogen starvation.The results indicated that it was feasible to utilize filamentous bacteria to accumulate PHA with a rate comparable to well-settled sludge.Furthermore,the lower dissolved oxygen demand of filamentous bacteria would save energy required for aeration in the culture enrichment stage. | Qinxue Wen Zhiqiang Chen Changyong Wang Nanqi Ren | 2012 | Journal of Environmental Sciences2012，24，10： | 15 |
| 2 | Effect of aeration rate on composting of penicillin mycelial dreg显示文摘Pilot scale experiments with forced aeration were conducted to estimate effects of aeration rates on the performance of composting penicillin mycelial dreg using sewage sludge as inoculation. Three aeration rates of 0.15, 0.50 and 0.90 L/(min·kg) organic matter(OM) were examined. The principal physicochemical parameters were monitored during the 32 day composting period. Results showed that the higher aeration rate of 0.90 L/(min·kg) did not corresponded to a longer thermophilic duration and higher rates of OM degradation;but the lower aeration rate of 0.15 L/(min·kg) did induce an accumulation of NH+4-N contents due to the inhibition of nitrification. On the other hand, aeration rate has little effect on degradation of penicillin. The results show that the longest phase of thermophilic temperatures ≥ 55°C, the maximum NO-3-N content and seed germination, and the minimum C/N ratio were obtained with 0.50 L/(min·kg) OM. Therefore, aeration rates of0.50 L/(min·kg) OM can be recommended for composting penicillin mycelial dreg. | Zhiqiang Chen Shihua Zhang Qinxue Wen Jun Zheng | 2015 | Journal of Environmental Sciences2015，27，11： | 13 |
| 3 | Effects of phosphorus and nitrogen limitation on PHA production in activated sludge显示文摘The effects of phosphorus and nitrogen limitation on polyhydroxyalkanoate(PHA) production and accumulation by activated sludge biomass with acetate as a carbon source were investigated.Pre-selected influent carbon-phosphorus(C:P,W/W) of 100,160,250,500 and 750,and carbon-nitrogen(C:N,W/W) of 20,60,100,125 and 180 were applied in the phosphorus limitation experiments and the nitrogen limitation experiments,respectively.The maximum PHA accumulation up to 59%of the cell dry weight with a PHA productivity of 1.61 mg PHA/mg COD consumed was observed at the C:N 125 in the nitrogen limitation experiment.This value was much higher than that obtained in previous studies with a normal substrate feeding.The study showed that activated sludge biomass would produce more polyhydroxybutyrate than polyhydroxyvalerate under the stress of nutrient limitation,especially under phosphorus limitation conditions.The experimental result also indicated that both phosphorus and nitrogen limitation may cause sludge bulking. | Qinxue Wen,Zhiqiang Chen,Ting Tian,Wei Chen State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China | 2010 | Journal of Environmental Sciences2010，22，10： | 9 |
| 4 | Efficient algorithm for 3D bimodulus structures显示文摘The bimodulus material is a classical model to describe the elastic behavior of materials with tension-compression asymmetry.Due to the inherently nonlinear properties of bimodular materials,traditional iteration methods suffer from low convergence efficiency and poor adaptability for large-scale structures in engineering.In this paper,a novel 3D algorithm is established by complementing the three shear moduli of the constitutive equation in principal stress coordinates.In contrast to the existing 3D shear modulus constructed based on experience,in this paper the shear modulus is derived theoretically through a limit process.Then,a theoretically self-consistent complemented algorithm is established and implemented in ABAQUS via UMAT;its good stability and convergence efficiency are verified by using benchmark examples.Numerical analysis shows that the calculation error for bimodulus structures using the traditional linear elastic theory is large,which is not in line with reality. | Qinxue Pan Jianlong Zheng Pihua Wen | 2020 | Acta Mechanica Sinica2020，36，1： | 2 |
| 5 | Mesophilic and thermophilic anaerobic digestion of swine manure with sulfamethoxazole and norfloxacin:Dynamics of microbial communities and evolution of resistance genes显示文摘The role of norfloxacin(NOR)and sulfamethoxazole(SMX)in mesophilic and thermophilic anaerobic digestion(AD)of pig manure,with respect to methane production and variations in the microbial community and resistance genes,including antibiotic resistance genes(ARGs),class I integrase(intIl),and heavy metal resistance genes(MRGs),was investigated.The results indicated that NOR exerted little influence on the microbial community,whereas SMX negatively affected the acetoclastic methanogens.The abundance of two sulfonamide resistance genes(sul1 and sul2),three quinolone resistance genes(qnrS,parC,and aac(6′)-Ib-cr),and intI1 decreased by 2–3 orders of magnitude at the end of thermophilic AD.In contrast,mesophilic AD was generally ineffective in reducing the abundance of resistance genes.According to the results of redundancy analysis,the abundance of ARGs was affected primarily by microbial community dynamics(68.5%),rather than the selective pressure due to antibiotic addition(13.3%).Horizontal gene transfer(HGT)through intI1 contributed to 26.4%of the ARG variation.The archaeal community also influenced the changes in the resistance genes,and ARG reduction was significantly correlated with enhanced methane production.Thermophilic AD presented a higher methane production potential and greater reduction in resistance gene abundance. | Qinxue Wen Shuo Yang Zhiqiang Chen | 2021 | Frontiers of Environmental Science & Engineering2021，15，5： | 2 |
| 6 | Effects of sludge retention time,carbon and initial biomass concentrations on selection process:From activated sludge to polyhydroxyalkanoate accumulating cultures显示文摘Four sequence batch reactors(SBRs) fed by fermented sugar cane wastewater were continuously operated under the aerobic dynamic feeding(ADF) mode with different configurations of sludge retention time(SRT), carbon and initial biomass concentrations to enrich polyhydroxyalkanoate(PHA) accumulating mixed microbial cultures(MMCs) from municipal activated sludge.The stability of SBRs was investigated besides the enrichment performance. The microbial community structures of the enriched MMCs were analyzed using terminal restriction fragment length polymorphism(T-RFLP). The optimum operating conditions for the enrichment process were: SRT of 5 days, carbon concentration of 2.52 g COD/L and initial biomass concentration of3.65 g/L. The best enrichment performance in terms of both operating stability and PHA storage ability of enriched cultures(with the maximum PHA content and PHA storage yield(YPHA/S) of61.26% and 0.68 mg COD/mg COD, respectively) was achieved under this condition. Effects of the SRT, carbon concentration and initial biomass concentration on the PHA accumulating MMCs selection process were discussed respectively. A new model including the segmentation of the enrichment process and the effects of SRT on each phase was proposed. | Zhiqiang Chen Long Huang Qinxue Wen Huichao Zhang Zirui Guo | 2017 | Journal of Environmental Sciences2017，29，2： | 2 |
| 7 | Reconsideration on the effect of nitrogen on mixed culture polyhydroxyaIkanoate production toward high organic loading enrichment history显示文摘In most of the operating strategies for mixed microbial cultures polyhydroxyalkanoate (PHA) production, moderate organic loads and low nitrogen concentrations are used, however, the real waste streams contain variable concentrations of carbon and nitrogen. To evaluate the effect of enrichment history on PHA producer and production the various carbon and nitrogen levels were utilized during the accumulation phase. Different operating strategies were applied in three sequencing batch reactors (SBRs) subjected to aerobic dynamic feeding. The maximum PHA production of the enriched cultures under nutrient excess, limitation and starvation (Cmol/Nmol ratio of & 40 and oo, respectively) was evaluated in batch assays. A higher PHA content and biomass growth were achieved in the nutrients presence in comparison to the nutrient starvation condition. The cultures from the SBR treated under short sludge retention time, high organic loading rate, short cycle length (SBR#3) and nutrient excess reached the maximum PHA content (54.9%) and biomass increase (38.9%). Under nutrient limitation, the negative biomass growth was observed under nutrient starvation because of the sampling loss. The succession of microbial communities in SBRs and batch assays was analyzed using terminal restriction fragment length polymorphism. The SBR#3 had the best overall PHA production performance considering its high PHA content and productivity in all nutrient content, it indicates that nitrogen has a substantial impact on PHA yield especially when high organic loading rate enrichment history is involved. | Zhiqiang Chen Lizhi Zhao Ye Ji Qinxue Wen Long Huang | 2019 | Frontiers of Environmental Science & Engineering2019，13，4： | 2 |
| 8 | Preparation and characterization of a lipoid adsorption material and its atrazine removal performance显示文摘A novel adsorbent named lipoid adsorption material (LAM), with a hydrophobic nucleolus (triolein) and a hydrophilic membrane structure (polyamide), was synthesized to remove hydrophobic organic chemicals (HOCs) from solution. Triolein, a type of lipoid, was entrapped by the polyamide membrane through an interfacial polymerization reaction. The method of preparation and the structure of the LAM were investigated and subsequent experiments were conducted to determine the characteristics of atrazine (a type of HOC) removal from wastewater using LAM as the adsorbent. The results showed that LAM had a regular structure compared with the prepolymer, where compact particles were linked with each other and openings were present in the structure of the LAM in which the fat drops formed from triolein were entrapped. In contrast to the atrazine adsorption behavior of powdered activated carbon (PAC), LAM showed a persistent adsorption capacity for atrazine when initial concentrations of 0.57, 1.12, 8.31 and 19.01 mg/L were present, and the equilibrium time was 12 hr. Using an 8 mg/L initial concentration of atrazine as an indicator of HOCs in aqueous solution, experiments on the adsorption capacity of the LAM showed 69.3% removal within 6-12 hr contact time, which was close to the 75.5% removal of atrazine by PAC. Results indicated that LAM has two atrazine removal mechanisms, namely the bioaccumulation of atrazine by the nucleous material and physical adsorption to the LAM membrane. Bioaccumulation was the main removal mechanism. | Zhiqiang Chen Qinxue Wen Jiaxiang Lian Nanqi Ren | 2011 | Journal of Environmental Sciences2011，23，8： | 1 |
| 9 | Impacts of advanced treatment processes on elimination of antibiotic resistance genes in a municipal waste water treatment plant显示文摘Antibiotic resistance genes (ARGs) pose a serious threat to public health. Wastewater treatment plants (WWTPs) are essential for controlling the release of ARGs into the environment. This study investigated ARG distribution at every step in the treatment process of a municipal WWTP located in Harbin for six consecutive months. Changes in ARG distribution involved in two advanced secondary effluent treatment processes, ozonation and granular activated carbon (GAC) adsorption, were analyzed. Biological treatment resulted in the highest ARG removal (0.76-1.94 log reduction), followed by ultraviolet (UV) disinfection (less than 0.5-log reduction). Primary treatment could not significantly remove ARGs. ARG removal efficiency increased with an increase in the ozone dose below 40 mg/L. However, amorphous GAC (AGAC) adsorption with a hydraulic retention time (HRT) of 1 h showed better removal of ARGs, total organic carbon (TOC), total nitrogen (TN), and total phosphorus (TP) than ozonation at a 60 mg/L dose. UV treatment could efficiently reduce the relative ARG abundance, despite presenting the lowest efficiency for the reduction of absolute ARG abundance compared with GAC and ozone treatments. The combination of ozone and AGAC can significantly improve the removal of ARGs, TOC, TN and TP. These results indicate that a treatment including biological processing, ozonation, and AGAC adsorption is a promising strategy for removing ARGs and refractory organic substances from sewage. | Lian Yang Qinxue Wen Zhiqiang Chen Ran Duan Pan Yang | 2019 | Frontiers of Environmental Science & Engineering2019，13，3： | 1 |
| 10 | Fate of pathogenicmicroorganisms and indicators in secondary activated sludgewastewater treatment plants显示文摘 | WEN Qinxue TUTUKA C KEEGAN A | 2009 | J Environ Manage2009，90，3： | 1 |
| 11 | Performance and Microbial Characteristics of Bioaugmentation Systems for Polyacrylamide Degradation显示文摘 | Qinxue Wen Zhiqiang Chen Ye Zhao | 2011 | Polym Environ2011，，19： | 1 |
| 12 | Biodegradation of polyacrylamide by bacteria isolated from activated sludge and oil-contaminated soil显示文摘 | Qinxue Wen Zhiqiang Chen Ye Zhao | 2010 | Journal of Hazardous Materials2010，，： | 1 |
| 13 | A new method for polyhydroxyalkanoate (PHA) accumulating bacteria selection under physical selective pressure 显示文摘 | Chen Zhiqiang Guo Zirui Wen Qinxue Huang Long Bakke R Du Maoan | 2015 | International Journal of Biological Macromolecules2015，72，： | 1 |
| 14 | Biodegradation of polyacrylamide by bacteria isolated from activated sludge and oil-contaminated soil显示文摘 | Qinxue Wen Zhiqiang Chen Ye Zhao Huichao Zhang Yujie Feng | 2009 | Journal of Hazardous Materials2009，，1： | 1 |
| 15 | Performance and Microbial Characteristics of Bioaugmentation Systems for Polyac- rylamide Degradation 显示文摘 | Qinxue Wen Zhiqiang Chen Ye Zhao | 2011 | J Polym Environ2011，，： | 1 |
| 16 | Performance and MicrobialCharacteristics of Bioaugmentation Systems for PolyacrylamideDegradation显示文摘 | Qinxue Wen Zhiqiang Chen Ye Zhao ex al | 2011 | J Polym Environ2011，，12： | 1 |
| 17 | Biodegrada-tion of polyacrylamide by bacteria isolated from activatedsludge and oil-contaminated soil 显示文摘 | WEN Qinxue CHEN Zhiqiang ZHAO Ye | 2010 | Journal ofHazardous Materials2010，，175： | 1 |
| 18 | Epidemiological,Clinical and Serological Characteristics of Children with Coronavirus Disease 2019 in Wuhan:A Single-centered,Retrospective Study显示文摘Dear Editor,In December 2019,SARS-Co V-2 was first detected in the samples obtained from three adult patients who suffered from an unknown viral pneumonia in Wuhan(Li et al.2020).This unknown viral pneumonia is further named as coronavirus disease 2019(COVID-19)by the World Health Organization. | Dan Luo Zhi Xia Heng Li Danna Tu Ting Wang Wei Zhang Lu Peng Wenfu Yi Sai Zhang Junhua Shu Hui Xu Yong Li Buyun Shi Chengjiao Huang Wen Tang Shuna Xiao Xiaolan Shu Yan Liu Yuan Zhang Shan Guo Zhi Yu Baoxiang Wang Yuan Gao Qinxue Hu Hanzhong Wang Xiaohui Song Hong Mei Xiaoqin Zhou Zhenhua Zheng | 2020 | Virologica Sinica2020，35，6： | 0 |
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