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| 1 | Growth inhibition and apoptosis induction Sulindac on Human gastric cancer cells显示文摘AIM: To evaluate the effects of sulindac in inducing growth inhibition and apoptosis of human gastric cancer cells in comparison with human hepatocellular carcinoma (HCC)cells.METHODS: The human gastric cancer cell lines MKN45 and MKN28 and human hepatocellular carcinoma cell lines HepG2and SMMC7721 were used for the study. Anti-proliferative effect was measured by MTT assay, and apoptosis was determined by Hoechst-33258 staining, electronography and DNA fragmentation. The protein of cyclooxygenase-2 (COX(2) and Bcl-2 were detected by Westem dot blotting.RESULTS: Sulindac could initiate growth inhibition and apoptosis of MKN45, MKN28, HepG2 and SMMC7721 cells in a dose-and time-dependent manner. Growth inhibitory activity and apoptosis were more sensitive in HepG2 cells than in SMMC7721 cells, MKN45 and MKN28 cells. After 24hours incubation with sulindac at 2mmol. L-1 and 4mmol.L-1, the level of COX-2 and Bcl-2 protein were lowered in MKN45, SMMC7721 and HepG2 cells but not in MKN28 cells.CONCLUSION: Sulindac could inhibit the growth of gastric cancer cells and HCC cells effectively in vitro by apoptosis induction, which was associated with regression of COX-2and Bcl-2 expression. The growth inhibition and apoptosis of HCC cells were greater then that of human gastric cancer cells. The different effects of apoptosis in gastric cancer cells may be related to the differentiation of the cells. | Yun-Lin Wu~1 Bo Sun~1 Xue-Jun Zhang~2 Sheng-Nian Wang~2 Heng-Yi He~2 Min-Min Qiao~1 Jie Zhong~1 Jia-Yu Xu~1 1 Department of Gastroenterology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China2 Institude of Biochemistry and Cell Biology,Shanghai Institues for Biological Sciences,Chinese Academy of Sciences.Shanghai 200025,China | 2001 | World Journal of Gastroenterology2001，7，6： | 64 |
| 2 | Immunohisto chemical Study on the Effects of Pyrethroids on Gluta-mate and γ-Aminobutyric Acid in Rat Central Nervous System显示文摘Objective\ In order to study the effects of pyrethroids on glutamate (Glu) and gamma aminobutyric acid (GABA) in rat central nervous system. Methods\ Male SD rats were treated with permethrin (7 mg/kg, i.p) or cypermethrin (70 mg/kg, i.p) once a day for 3 days. Glu and GABA immunoreactive cells and transmitters in CNS were studied by immunohistochemical method. Results\ The total number, percentage of positive area and integral optical density of glutamate immunoreactive cell in cerebral cortex, hippocampus decreased significantly in permethrin or cypermethrin, while GABA was enhanced. Cypermethrin is more tent than permethrin on either Glu or GABA. Conclusion\ Disturbance of Glu and GABA is likely to play a role in the development of pyrethroids excitatory neurotoxicity. | Wu Jianping, Lu Chun\+1, Wang Ying, et al. Wu Jianping, Lu Chun\+1, Wang Ying, et al. Institute of Toxicology, Nanjing Medical University, Nanjing\ 210029 Acta Universitatis Medicinalis Nanjing,19(6):450 | 1999 | The Journal of Biomedical Research1999，23，2： | 68 |
| 3 | The vegetation and climate change during Neocene and Early Quaternary in Jiuxi Basin, China显示文摘Sporopollen record in the Laojunmiao Section at Yumen in the Hexi Corridor foreland depression at the northern margin of the Tibetan Plateau revealed that during the period of 13.0―11.15 Ma the ecological environment of the Jiuxi Basin is characterized by steppe vegetation and a semi-moist climate. During 11.16―8.60 Ma prevailed forests of cypress and a still warmer, moister climate; steppe vegetation and dry climate began probably at about 8.6 Ma. Although aridification had been relaxed time and again during 8.40―6.93 Ma (forest-steppe, warm-semi-moist), 6.64―5.67 Ma (open-forest and steppe, warmer-semi-moist) and 5.42―4.96 Ma (steppe, semi-arid), the climate in the region became drier and drier in response to the fre- quent occurrence of aridity during 6.93―6.64 Ma (steppe, semi-arid), 5.67―5.42 Ma (de- sert-steppe, arid), 3.66―3.30 Ma (desert-steppe, arid) and 2.56―2.21 Ma (desert, arid). Perhaps the important findings of our study are the notable expansion of drought-enduring plants during 3.66―3.30 Ma and about 2.56 Ma and the replacement of vegetation by vast arid desert. | MA Yuzhen 1 , FANG Xiaomin 1,2 , LI Jijun 1 , WU Fuli 1 & ZHANG Jun 1 1. Key Laboratory of Western China’s Environmental Systems and College of Resources and Environment, Lanzhou University, Lanzhou 730000, China 2. State Key Laboratory of Loess and Quaternary Geology, Institute of Earth Environment, Chinese Academy of Sciences, Xi’an 710075, China | 2005 | Science China Earth Sciences2005，48，5： | 31 |
| 4 | Hyaluronic acid fragments evoke Kupffer cells via TLR4 signaling pathway显示文摘Kupffer cells, expressing toll-like receptor 4 (TLR4), play a central role in hepatic ischemia/reperfusion (I/R) injury. Hyaluronic acid (HA) fragments, degradative products of high-molecular-weight HA (HMW-HA), acquire the ability to activate immune cells under inflammatory conditions. Here we inves- tigated whether HA fragments could activate Kupffer cells and analyzed the underlying mechanism. Kupffer cells were isolated from wild-type mice (WT, C3H/HeN) and TLR4 mutant mice (C3H/HeJ) and HA fragments were produced by the methods of enzyme digestion and chromatography. Then Kupffer cells were stimulated by HA fragments or other control stimuli. The activation of Kupffer cells was estimated as the release of pro-inflammatory cytokines. The activation of p38 MAPK pathway of Kupffer cells was checked and blocking experiments were done as well. The results indicated that HA fragments acquired the ability to activate Kupffer cells in vitro, which was TLR4 dependent and not due to contamination of lipopolysaccharide. Experiments of p38 MAPK kinase inhibition by SB-203580 verified p38 MAPK was required in HA fragments induced Kupffer cells activation. This suggests that HA fragments, degradative products of one of the major glycosaminoglycans of the extracellular matrix, play critical roles in Kupffer cell activation mediated by TLR4 signaling pathway, which is, at least partially, de- pendent on p38 MAPK activation. | ZHANG JinXiang1, WANG Hui2, XIAO Qing3, LIANG HuiFang4, LI ZhuoYa4, JIANG ChunFang1, WU HeShui5 & ZHENG QiChang5 1 Department of Emergency Surgery, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430022, China 2 Department of Medical Genetics, Tongji Medical College Affiliated to Huazhong University of Science and Technology, Wuhan 430030, China 3 Department of Ophthalmology, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430022, China 4 Department of Medical Immunology, Tongji Medical College Affiliated to Huazhong University of Science and Technology, Wuhan 430030, China 5 Department of General Surgery, Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430022, China | 2009 | Science China(Life Sciences)2009，52，2： | 33 |
| 5 | Mechanism of the Spring Persistent Rains over southeastern China显示文摘The Spring Persistent Rains (SPR) in the areas to the south of middle and lower reaches of the Yangtze River or over southeastern China (SEC) is a unique synoptic and climatic phenomenon in East Asia. This study reveals a possible mechanism responsible for the climatic cause of SPR formation through climatic mean data analysis and sensitive numerical model experiments. SEC is located at the down-stream of the southwesterly velocity center (SWVC) which lies on the southeastern flank of the Tibetan Plateau (TP). As a result, there are strong southwesterly wind velocity convergence and moisture con-vergence over SEC. This is the immediate climatic cause of SPR formation. In spring, the seasonal evolution of the southwesterly velocity consists with the surface sensible heating over southeastern TP, indicating that the formation of SPR is related to not only the southwesterly wind of mechanical de-flected flow of TP, but also the southwesterly wind of thermal-forced cyclonic low circulation. Sensitive numerical experiments demonstrate that, without TP, both SWVC and the SPR rain belt will disappear. The southwesterly wind velocity increases almost linearly with the amount of the total diabatic heating with TP rising. Therefore, SWVC is the result of the mechanical forcing and thermal forcing of TP. All these strongly suggest that the presence of TP plays a primary role in the climatic formation of SPR. | WAN RiJin1,2,3 & WU GuoXiong1 1 State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Mechanicals (LASG), Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China 2 Guangdong Climate and Agrometeorology Center, Guangzhou 510080, China 3 Shanghai Typhoon Institute of China Meterological Administration, Shanghai 200030, China | 2007 | Science China Earth Sciences2007，50，1： | 34 |
| 6 | Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.)显示文摘To understand the development of rice leaf blades,we identified a new rolled-leaf mutant,w32,from indica cultivar IR64 through EMS mutagenesis. The mutant showed a stable rolled-leaf phenotype throughout the life cycle. Two F2 populations were developed by crossing w32 to cultivar IR24 and PA64. Genetic analysis showed that the rolled-leaf phenotype was controlled by a single recessive gene. To determine the location of the gene,bulked segregant analysis was carried out using mutant and wild-type DNA pools and 1846 mutant-type F2 individuals derived from the cross w32/PA64 were genotyped to locate the gene on the short arm of chromosome 7. The rolled-leaf gene,tentatively named rl11(t),is likely a new gene as no other rolled-leaf genes have been identified near the region. By developing new SSR and InDel markers,the gene was delimited to a 52 kb region near the end of the short chromosome arm. Further fine mapping and cloning of the gene are currently underway. | SHI YongFeng1,2,CHEN Jie1,LIU WenQiang1,HUANG QiNa1,2,SHEN Bo2,LEUNG Hei3 & WU JianLi1 1 Chinese National Center for Rice Improvement/National key Laboratory for Rice Biology,China National Rice Research Institute,Hangzhou 310006,China 2 College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 310016,China 3 International Rice Research Institute,Metro Manila DAPO Box 7777,Philippines | 2009 | Science China(Life Sciences)2009，52，9： | 29 |
| 7 | Review on hidden trouble detection and health diagnosis of hydraulic concrete structures显示文摘A large number of hydraulic concrete structures have hidden defects such as cracks, erosion, freeze and thaw, thermal fatigue, carbonization. These hidden defects seriously affect the strength, stability and durability of structures. These problems are studied mainly by single monitoring or diagnosis methods at present. The integration of multiple monitoring and diagnosis methods is not applied widely. Besides, the analysis theory on these problems is not developed very well. The systemic study on the aging mechanism of hydraulic concrete structures, time- variation model and health diagnosis is still not enough. The support for engineering practice is limited. Aimed at these major scientific and technological problems and combined with specific projects, study on detection of hidden defects and health diagnosis of hydraulic concrete structure has been carried out. This study includes the following content: field non-destructive examination of hidden defects of hydraulic concrete structures, seepage detection, the construction of in-situ sensing system, the combination of field detection and in-situ monitoring, the mechanism of crack, freeze and thaw, erosion and carbonization of hydraulic concrete structure, mechanism of combination aging; time-variation model of hydraulic concrete structure, theories and methods for health diagnosis of hydraulic concrete structures. | WU ZhongRu1,2?, LI Ji1, GU ChongShi1,2 & SU HuaiZhi1,2 1 College of Water Conservancy and Hydropower Engineering, Hohai University, Nanjing 210098, China 2 National Engineering Research Center of Water Resources Efficient Utilization and Engineering Safety, Nanjing 210098, China | 2007 | Science China(Technological Sciences)2007，50，z1： | 27 |
| 8 | Production of proinflammatory cytokines in the human THP-1 monocyte cell line following induction by Tp0751,a recombinant protein of Treponema pallidum显示文摘The tissue destruction characteristic of syphilis infection may be caused by inflammation due to Treponema pallidum and the ensuing immune responses to the pathogen.T.pallidum membrane proteins are thought to be potent inducers of inflammation during the early stages of infection.However,the actual membrane proteins that induce inflammatory cytokine production are not known,nor are the molecular mechanisms responsible for triggering and sustaining the inflammatory cascades.In the present study,Tp0751 recombinant protein from T.pallidum was found to induce the production of proinflammatory cytokines,including TNF-α,IL-1βand IL-6,in a THP-1 human monocyte cell line.The signal transduction pathways involved in the production of these cytokines were then further investigated.No inhibition of TNF-a,IL-1β,or IL-6 production was observed following treatment with the SAPK/JNK specific inhibitor SP600125 or with an ERK inhibitor PD98059.By contrast,anti-TLR2 mAb,anti-CD14 mAb,and the p38 inhibitor SB203580 significantly inhibited the production of all three cytokines.In addition,pyrrolidine dithiocarbamate (PDTC),a specific inhibitor of NF-κB,profoundly inhibited the production of these cytokines.Tp0751 treatment strongly activated NF-κB,as revealed by Western blotting.However,NF-κB translocation was significantly inhibited by treatment with PDTC.These results indicated that TLR2,CD14,MAPKs/p38,and NF-κB might be implicated in the inflammatory reaction caused by T.pallidum infection. | LIU ShuangQuan1,2,WANG ShiPing1,WU YiMou3,ZHAO FeiJun3,ZENG TieBing3,ZHANG YueJun3,ZHANG QiuGui2 & GAO DongMei1 1 Department of Parasitology,Xiangya School of Medicine,Central South University,Changsha 410078 ,China 2 The First Affiliated Hospital,University of South China,Hengyan 421001,China 3 Department of Microbiology and Immunology,University of South China,Hengyan 421001,China | 2010 | Science China(Life Sciences)2010，53，2： | 28 |
| 9 | Application and study in aluminum melt degassing technologies显示文摘The classification, characteristics and current situation of aluminum melt hydrogen-removal methods and technologies have been summarized. The latest studies and research results of aluminum melt degassing technology have been summed up. The tendency and prospect of aluminum degassing technology was discussed. | ~*H. J. Ni~(1,2), M. Y. Huang~1, Y. Zhu~1, P. Liao~1, R. Z. WU~2, B. D. Sun~2(1. Department of Mechanical Engineering, Nantong Institute of Technology, Nantong 226007, Jingsu, P. R. China 2. School of Material Science and Engineering, Shanghai Jiaotong University, Shanghai 200030, P. R. China) | 2004 | China Foundry2004，1，S1： | 27 |
| 10 | Deficit of mitochondria-derived ATP during oxidative stress impairs mouse MII oocyte spindles显示文摘尽管在母亲的老化和不孕的氧化应力的角色被建议了,内在的机制充分没被理解。现在的学习被设计在氧化应力下面在中期 II (MII ) 卵母细胞决定在 mitochondrial 功能和锭子稳定性之间的关系。MII 老鼠卵母细胞当面与 H2O2 被对待或渗透转变毛孔(PTP ) 的缺席块 ers cyclosporin A (CsA ) 。另外,抗氧化剂 N-acetylcysteine (NAC ) , F0/F1 synthase 禁止者 oligomycin A,线粒体 uncoupler 羰基氰化物 4-trifluoro-methoxyphenylhydrazone (FCCP ) 或加 2.5 公里 Ca2+(Th+2.5 公里 Ca2+) 的 thapsigargin 在机械学的研究被使用。卵母细胞锭子和染色体的词法分析被执行并且 mitochondrial 膜潜力(DeltaPsim ) ,细胞质的免费的钙集中([Ca2+] c ) 并且在卵母细胞以内的细胞质的 ATP 内容也是 assayed。在一时间 -- 并且 H2O2 剂量依赖者举止,锭子在卵母细胞以后被发现的 meiotic 的混乱与 H2O2 被对待,它被预告的处理与 NAC 阻止。H2O2 的管理导致了 DeltaPsim 的驱散,的增加[Ca2+] 在细胞质的 ATP 的 c 和减少铺平。到 H2O2 处理的卵母细胞的这些有害回答能被孵化前与 CsA 堵住。类似于驱散的 H2O2,两 oligomycin A 和 FCCP DeltaPsim,减少的细胞质的 ATP 内容和拆卸的 MII 卵母细胞锭子,当时高[Ca2+] c 独自没在锭子形态学上有效果。在结论,在导出线粒体的 ATP 的减少可以在氧化应力期间引起老鼠 MII 卵母细胞锭子的拆卸,大概由于 mitochondrial PTP 的开始。 | Xiao Zhang~(1,2,*) Xue Qing Wu~(1,3,*) Shuang Lu~(4,5,6) Ying Lu Guo~1 Xu Ma~(4,5,6) ~1Peking University First Hospital,Institute of Urology of Peking University,Beijing 100034,China ~2Reproductive and Genetic Center of Peking University First Hospital,Beijing 100034,China ~3Department of Obstetrics and Gynecology,Shanxi Medical University Second Hospital,Taiyuan 030000,China ~4Reproductive and Genetic Center of National Research Institute for Family Planning,Beijing 100038,China ~5Department of Genetics, Peking Union Medical College, Beijing 100038. China ~6 WHO Collaborative Center for Research in Human Reproduction,Beijing 100038,China | 2006 | Cell Research2006，16，10： | 24 |
| 11 | Numerical simulations of self-propelled swimming of 3D bionic fish school显示文摘Numerical simulations of self-propelled swimming of a three dimensional bionic fish and fish school in a viscous fluid are carried out. This is done with the assistance of a parallel software package produced for 3D moving boundary problems. This computational fluid dynamics package combines the adaptive multi-grid finite volume method, the immersed boundary method and VOF (volume of fluid) method. By using the package results of the self-propelled swimming of a 3D bionic fish and fish school in a vis- cous fluid are obtained. With comparison to the existing experimental measurements of living fishes, the predicted structure of vortical wakes is in good agreement with the measurements. | WU ChuiJie1 & WANG Liang2 1 State Key Laboratory of Structural Analysis for Industrial Equipment, Dalian University of Technology, Dalian 116024, China 2 Research Center for Fluid Dynamics, People’s Liberation Army University of Science and Technology, Nanjing 211101, China | 2009 | Science China(Technological Sciences)2009，52，3： | 23 |
| 12 | Reconstructing mean maximum temperature of growing season from the maximum density of the Schrenk Spruce in Yili,Xinjiang,China显示文摘To study the historical temperature variation in Yili of Xinjiang,the tree-ring chronologies of maximum density(MXD) and tree-ring width(TRW) were established with the Schrenk Spruce(Picea schrenkiana Fisch.et Mey) collected from six sites to analyze the characteristics and ability of response to climate change.The results suggest that the MXD chronology of the Schrenk Spruce from different sampling sites responded to climate change well and were positively correlated with the mean maximum temperature and the mean temperature from April to August in the study area.The mean maximum temperature anomaly from April to August,spanning the years from 1848 to 2000,was reconstructed by three MXD chronologies which were selected by stepwise regression.The reconstructed function was stable and explained 56.2% of the variance.The reconstructed results indicated a cold span of 153 years in spring and summer from the beginning of the 1950s to the beginning of the 1970s in the Yili valley.No strongly increasing tendency was detected in the mean maximum temperature anomaly from April to August during this reconstructed period. | CHEN Jin1,2,3,WANG LiLi1,4,5,ZHU HaiFeng1 & WU Pu1 1 Institute of Geographic Sciences and Natural Resources Research,Chinese Academy of Sciences,Beijing 100101,China 2 Graduate University of Chinese Academy of Sciences,Beijing 100049,China 3 Institute of Vertebrate Paleontology and Paleoanthropology,Chinese Academy of Sciences,Beijing 100044,China 4 Institute of Tibetan Plateau Research,Chinese Academy of Sciences,Beijing 100085,China 5 Institute of Desert and Meteorology,China Meteorological Administration,ürümqi 830002,China | 2009 | Chinese Science Bulletin2009，54，13： | 21 |
| 13 | The use of laser ranging to measure space debris显示文摘Space debris is a major problem for all the nations that are currently active in space.Adopting high-precision measuring techniques will help produce a reliable and accurate catalog for space debris and collision avoidance.Laser ranging is a kind of real-time measuring technology with high precision for space debris observation.The first space-debris laser-ranging experiment in China was performed at the Shanghai Observatory in July 2008 with a ranging precision of about 60-80 cm.The experimental results showed that the return signals from the targets with a range of 900 km were quite strong,with a power of 40W (2J at 20 Hz) using a 10ns pulse width laser at 532 nm wavelength.The performance of the preliminary laser ranging system and the observed results in 2008 and 2010 are also introduced. | Zhong-Ping Zhang 1,2,Fu-Min Yang 1,Hai-Feng Zhang 1,Zhi-Bo Wu 1,2,Ju-Ping Chen 1,2,Pu Li 1 and Wen-Dong Meng 1 1 Shanghai Astronomical Observatory,Chinese Academy of Sciences,Shanghai 200030,China 2 Key Laboratory of Space Object and Debris Observation,Chinese Academy of Science,Nanjing 210008,China | 2012 | Research in Astronomy and Astrophysics2012，12，2： | 20 |
| 14 | Theory on real-time control of construction quality and progress and its application to high arc dam显示文摘A complete scheme for solving the key scientific problems associated with high-standard,high-intensity continuous construction of high arch dams was presented. First,based on a coupling analysis of construction system decomposition and coordination for a high arc dam,a mathematical model for real-time control of construction quality and progress that considers complex constraints was developed. Second,a method of progress control was proposed based on a dynamic simulation. Third,a dynamic quality control mechanism was established based on construction information collected using a PDA. Fourth,a system for integrating collected information,progress simulation and quality control analyses under a network environment was developed. Finally,these methods were applied to a practical project to show that each aspect of a construction process can be managed effectively and that real-time monitoring and feedback control can be realized. Our methods provide new theoretical principles and technical measures for quality and progress control in the high arc dam construction process. | ZHONG DengHua1, REN BingYu1, LI MingChao1, WU BinPing1 & LI MingChuan2 1 Department of Hydraulic and Hydroelectric Engineering, School of Civil Engineering, Tianjin University, Tianjin 300072, China 2 Ertan Hydropower Development Company, Ltd., Chengdu 610051, China | 2010 | Science China(Technological Sciences)2010，53，10： | 18 |
| 15 | 新疆望峰金矿成矿流体研究及其成因意义显示文摘新疆望峰金矿,产于中天山结晶基底中的韧性剪切带内。围岩蚀变和流体包裹体研究表明,成矿过程包括了3个阶段,从早到晚有石英黄铁矿阶段、多金属硫化物阶段和石英碳酸盐阶段,温度分别集中在360~520℃、240~360℃和120~240℃;矿化以第Ⅱ阶段为主。成矿流体的δ18O值变化于1.15‰~2.70‰之间,属于变质热液与大气降水热液的混合流体。包裹体成分表明成矿流体来源较浅,但有利于金矿化;CO2/H2O比值多大于0.026,也有利于成矿。而包裹体捕获压力表明成矿作用发生在5.6~1.2km深度范围,主要发生在1.9km左右;矿化过程与地壳抬升伸展的过程一致,自然亦与碰撞造山体制的矿化规律一致。因此,古生代末期的碰撞造山作用导致了望峰金矿的形成,冰达坂断裂两侧是寻找望峰式金矿的有利地带。石英红外光谱分析表明,CO2光密度(D2)及其与H2O光密度(D1)的比值(D2/D1)普遍较高,指示矿化较强;D2>0.55和D2/D10.26是该区富金样品的标志。 | Chen Yanjing 1) Li Xin 1) Jing Jun 2) Gao Xiuli 1) Chen Huayong 1) Wu Xidan 2) Wu Dehua 2) ( 1) Department of Geology, Peking University 2)Team 15 of Gold Headquarters, MMIC, Urumqi ) | 1998 | 地球学报(中国地质科学院院报)1998，19，2： | 18 |
| 16 | Impacts of increased atmospheric CO_2 concentration on photosynthesis and growth of micro-and macro-algae显示文摘Marine photosynthesis drives the oceanic biological CO2 pump to absorb CO2 from the atmosphere, which sinks more than one third of the industry-originated CO2 into the ocean. The increasing atmos-pheric CO2 and subsequent rise of pCO2 in seawater, which alters the carbonate system and related chemical reactions and results in lower pH and higher HCO3- concentration, affect photosynthetic CO2 fixation processes of phytoplanktonic and macroalgal species in direct and/or indirect ways. Although many unicellular and multicellular species can operate CO2-concentrating mechanisms (CCMs) to util-ize the large HCO3- pool in seawater, enriched CO2 up to several times the present atmospheric level has been shown to enhance photosynthesis and growth of both phytoplanktonic and macro-species that have less capacity of CCMs. Even for species that operate active CCMs and those whose photo-synthesis is not limited by CO2 in seawater, increased CO2 levels can down-regulate their CCMs and therefore enhance their growth under light-limiting conditions (at higher CO2 levels, less light energy is required to drive CCM). Altered physiological performances under high-CO2 conditions may cause genetic alteration in view of adaptation over long time scale. Marine algae may adapt to a high CO2 oceanic environment so that the evolved communities in future are likely to be genetically different from the contemporary communities. However, most of the previous studies have been carried out under indoor conditions without considering the acidifying effects on seawater by increased CO2 and other interacting environmental factors, and little has been documented so far to explain how physi-ology of marine primary producers performs in a high-CO2 and low-pH ocean. | WU HongYan1, ZOU DingHui1 & GAO KunShan2 1 Marine Biology Institute, Shantou University, Shantou 515063, China 2 State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China | 2008 | Science China(Life Sciences)2008，51，12： | 18 |
| 17 | Chinese HJ-1A/B satellites and data characteristics显示文摘From the viewpoint of environmental remote-sensing applications,this article explains the overall technical characteristics of the Chinese HJ-1A and HJ-1B satellites.It also investigates the spectral characteristics and potential applications of charge-coupled devices,and the infrared and hyperspectral data obtained by the satellites.Examples of applications,such as the remote-sensing monitoring of algal bloom in Taihu Lake,straw burning in southern China,and aerosol optical depth in the area around Bohai sea are presented.These examples illustrate the application characteristics of the HJ-1A and HJ-1B satellite data. | WANG Qiao1,WU ChuanQing1,LI Qing1 & LI JunSheng2 1 Satellite Environment Center,Ministry of Environmental Protection,Beijing 100029,China 2 Center for Earth Observation and Digital Earth,Chinese Academy of Sciences,Beijing 100190,China | 2010 | Science China Earth Sciences2010，53，S1： | 17 |
| 18 | Performance prediction and flow analysis in the vaned distributor of a pump turbine under low flow rate in pump mode显示文摘The main goal of this work is to investigate the possible different flow patterns existing in pump turbine under off-design conditions in pump mode. Numerical simulations by solving the Navier-Stokes equation, coupled with the 'SST k-ω' turbulence model, were carried out. Flow characteristics were assumed to be stalled in the appropriate region of ?ow rate levels of Q/QD=0.15–0.61. The simulation result was compared with experimental data and they showed good agreement. Consequently, velocity fields in three axial locations in stay vanes and guide vanes were analysed in details. It was shown that 'jet-wake' flow pattern exists near the band, which changes little in the whole shape with flow rate increasing; to the middle location of vanes, reverse flow begins to appear on the interface between the runner and guide vanes, which will disappear gradually as the flow rate increases; massive reverse flow is captured near the crown, whose intensity will be weakened as the flow rate increases. Ultimately, it was found that the special head-flow profile can be ascribed to the special hydraulic loss characteristics of the stay vanes and guide vanes. | YIN JunLian1, LIU JinTao1, WANG LeQin1, JIAO Lei1, WU DaZhuan1 & QIN DaQing2 1 Institute of Chemical Machinery, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China 2 Harbin Institute of Large Electrical Machinery, Harbin 150001, China | 2010 | Science China(Technological Sciences)2010，53，12： | 17 |
| 19 | Orbit determination and prediction accuracy analysis for a regional tracking network显示文摘China's COMPASS satellite navigation system relies on a regional tracking network to provide navigation services. Limited by its geographic border,the regional network is able to cover only 30% of the medium-earth-orbits(MEO). Accuracy of determined and predicted orbits is not able to satisfy system requirements if the tracking data processing strategy for global tracking network processing is used for the regional network. Two major error sources for orbital prediction are accuracy of initial orbital elements and dynamical modeling. To achieve better prediction accuracy,we propose a two-step orbit determination and prediction strategy. For step 1,only solar radiation pressure(SRP) parameters are estimated along with the orbital elements and other parameters; for step 2,all parameters are estimated but the SRP parameters are tightly constrained to their step 1 estimates. Experimenting with data from a regional GPS network,we conclude for orbital prediction using the proposed two-step strategy,the average user range error(URE) for 24-h prediction arcs is better than 0.6 m. | ZHOU ShanShi 1,2,3 ,HU XiaoGong 1 & WU Bin 1 1 Shanghai Astronomical Observatory,Chinese Academy of Sciences,Shanghai 200030,China 2 Graduate University of Chinese Academy of Sciences,Beijing 100049,China 3 Research Center of GNSS,Wuhan 430079,China | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，6： | 16 |
| 20 | Immunization with chlamydial plasmid protein pORF5 DNA vaccine induces protective immunity against genital chlamydial infection in mice显示文摘To validate the immune protective efficacy of pORF5 DNA vaccine and to analyze potential mechanisms related to this protection. In this study, pORF5 DNA vaccine was constructed and evaluated for its protective immunity in a mouse model of genital chlamydial infection. Groups of BALB/c mice were immunized intranasally with pORF5 DNA vaccine. Humoral and cell mediated immune responses were evaluated. The clearance ability of chlamydial challenge from the genital tract and the chlamy- dia-induced upper genital tract gross pathology and histopathological characterization were also de- tected. The results showed that the total and the IgG2a anti-pORF5 antibody levels in serum were sig- nificantly elevated after pcDNA3.1-pORF5 vaccination, as were the total antibody and IgA levels in vaginal fluids. pcDNA3.1-pORF5 induced a significantly high level of Th1 response as measured by robust gamma interferon (IFN-γ). Minimal IL-4 was produced by immune T cells in response to the re-stimulation with pORF5 protein or the inactive elementary body in vitro. pcDNA3.1-pORF5-vacci- nated mice displayed significantly reduced bacterial shedding upon a chlamydial challenge and an accelerated resolution of infection. 100% of pcDNA3.1-pORF5 vaccinated mice successfully resolved the infection by day 24. pcDNA3.1-pORF5-immunized mice also exhibited protection against patho- logical consequences of chlamydial infection. The stimulated index was significantly higher than that of mice immunized with pcDNA3.1 and PBS (P<0.05). Together, these results demonstrated that immu- nization with pORF5 DNA vaccine is a promising approach for eliciting a protective immunity against a genital chlamydial challenge. | LI ZhongYu1,2, WANG ShiPing1, Wu YiMou2, ZHONG GuangMing3 & CHEN Ding3 1 Xiangya School of Medicine, Central South University, Changsha 410078, China 2 Department of Microbiology and Immunology, University of South China, Hengyang 421001, China 3 University of Texas Health Science Center at San Antonio, TX 78229, USA | 2008 | Science China(Life Sciences)2008，51，11： | 16 |
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