Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.)

查看全文 作　　者：SHI YongFeng1,2,CHEN Jie1,LIU WenQiang1,HUANG QiNa1,2,SHEN Bo2,LEUNG Hei3 & WU JianLi1 1 Chinese National Center for Rice Improvement/National key Laboratory for Rice Biology,China National Rice Research Institute,Hangzhou 310006,China;2 College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 310016,China;3 International Rice Research Institute,Metro Manila DAPO Box 7777,Philippines

出　　处：《Science China(Life Sciences)》索引2009年第52卷第9期,共6页

基　　金：Supported by the National High Technology Research and Development Program of China (Grant Nos. 2006AA10Z1E8 and 2006AA100101) 摘　　要：To understand the development of rice leaf blades,we identified a new rolled-leaf mutant,w32,from indica cultivar IR64 through EMS mutagenesis. The mutant showed a stable rolled-leaf phenotype throughout the life cycle. Two F2 populations were developed by crossing w32 to cultivar IR24 and PA64. Genetic analysis showed that the rolled-leaf phenotype was controlled by a single recessive gene. To determine the location of the gene,bulked segregant analysis was carried out using mutant and wild-type DNA pools and 1846 mutant-type F2 individuals derived from the cross w32/PA64 were genotyped to locate the gene on the short arm of chromosome 7. The rolled-leaf gene,tentatively named rl11(t),is likely a new gene as no other rolled-leaf genes have been identified near the region. By developing new SSR and InDel markers,the gene was delimited to a 52 kb region near the end of the short chromosome arm. Further fine mapping and cloning of the gene are currently underway. 关键词：RICE (Oryza SATIVA L.) chemical MUTAGENESIS molecular marker rolled-leaf gene physical map

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Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.)

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