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| 1 | Banxia Xiexin Decoction(半夏泻心汤)Treats Diabetic Gastroparesis through PLC-IP3-Ca^2+/NO-cGMP-PKG Signal Pathway显示文摘Objective To test the effect of Banxia Xiexin Decoction(半夏泻心汤,BXD)on the contraction and relaxation of gastric smooth muscle(SM)in diabetic gastroparesis(DGP)model rats,and to explore the mechanism of BXD in the prevention and treatment of DGP through experiments of signal pathway both in vivo and in vitro.Methods Sixty Sprague-Dawley rats were divided into 6 groups according to a random number table:control group,model group,high-,medium-and low-dose BXD groups(9.2,4.6 and 1.8 g/(kg·d),respectively),and domperidone group(10 mg/(kg·d)),10 rats per group.DGP model was established initially by a single intraperitoneal injection of streptozotocin(STZ),and was confirmed by recording gastric emptying,intestinal transport velocity and gastric myoelectric activity of rats after 2 months.Each group was treated with a corresponding drug for 4 weeks.The mRNA and protein expressions of phospholipase C(PLC),inositol triphosphate(IP3),neuronal nitric oxide synthase(nNOS),and cyclic guanosine monophosphate(cGMP)dependent protein kinase G(PKG)were detected by reverse transcription-polymerase chain reaction and Western blot,respectively,while nitric oxide(NO)and cGMP expressions were detected by enzyme-linked immunosorbent assay.Gastric tissues were obtained from rats for primary cell culture preparation.Gastric SM cells were treated with 0.8µmol/L of STZ or STZ plus 1,000,500 and 200µg/mL of BXD or STZ plus 2.5µmol/mL of domperidone for 24,48,72 or 96 h,respectively.The length of gastric SM cells and intracellular Ca^2+concentration([Ca^2+]i)before and after BXD treatment was measured.Results Compared with the model group,high-and medium-dose BXD and domperidone significantly increased the expressions of PLC,IP3,NO,nNOS,cGMP and PKG in rat’s gastric tissue(P<0.01).Gastric SM cells treated with BXD showed a time-and dose-dependent increase in cell viability(P<0.01).The treatment with high-and medium-dose BXD and domperidone inhibited the increase in gastric SM cells length and increased[Ca^2+]i compared with the model cells(P<0.01).Conclusions Treatment with high-and medium-dose BXD significantly attenuated STZ-induced experimental DGP in rats.The therapeutic effect of BXD on DGP rats might be associated with the PLC-IP3-Ca^2+/NO-cGMP-PKG signal pathway. | WANG Bin ZENG Ke-wu HONG Zi-fu Tl Gui-xiang WANG Li-yun LU Pin LIU Zhen | 2020 | Chinese Journal of Integrative Medicine2020，26，11： | 8 |
| 2 | Expression and release of IL-29 by mast cells and modulation of mast cell behavior by IL-29显示文摘 | He, S Zhang, H Chen, H Yang, H Huang, T Chen, Y Lin, J Wang, F Chen, X Li, TL Yang, P | 2010 | 南京医科大学学报(自然科学版)2010，30，11： | 6 |
| 3 | Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells显示文摘 | Hsu, TL Hanson, SR Kishikawa, K Wang, SK Sawa, M Wong, CH | 2007 | 中国生物学文摘2007，21，10： | 5 |
| 4 | Effects of Zr on Microstructure and Short-TermStrength in GH586显示文摘0.020, 0.045 and 0.075 wt pct Zr were added to a Ni-base superalloy GH586 to investigate theirefFects on the microstructure and the short-term strengths at 850℃. The results showed thatproper Zr addition could improve the alloy tensile strength at 850℃ and short-term rupture life at850℃, 580 MPa. Zr exists in both γ’ phase and γ matrix, which caused Zr to distribute in graininstead of segregating in grain boundaries, therefore increased both of their lattice parametersbut decreased the mismatch between them. Meanwhile, it also increased the amount of grain-boundary carbides and decreased their size markedly. These contributed to the improvement ofshort-term rupture life. However, further addition of Zr resulted in forming Zr4C2S2, a plate-fike stable sulphurocarbides in grain boundaries, which decreased short-term strength at hightemperature, and led to a worse hot workability. According to the experimental results, theoptimum content of Zr should be 0.020 wt pct. | Xie, SS Wang, TL Lu, JY Yang, HC Zhao, GP | 1999 | Journal of Materials Science & Technology1999，15，5： | 3 |
| 5 | Risk factors and mortality in patients with nosocomial Staphylococcus aureus bacteremia显示文摘 | Wang FD Chen YY Chen TL | 2008 | Am J Infect Control2008，36，2： | 1 |
| 6 | Triglyceride-rich lipoprotein lipolysis releases neutral and oxidized FFAs that induce endothelial cell inflammation 显示文摘 | Wang L Gill R Pedersen TL | 2009 | J Lipid Res2009，50，2： | 1 |
| 7 | Exposure of single-stranded telomefic DNA causes G2/M cell cycle arrest in saccharomyces cerevisiae显示文摘 | Pang TL Wang CL Hsu CL | 2003 | J Biol Chem2003，278，11： | 1 |
| 8 | Notch signaling,gamma-secretase inhibitors,and cancer therapy显示文摘 | Shih IeM Wang TL | 2007 | Cancer Res2007，67，5： | 1 |
| 9 | Interleukin - 8 A Mitogcn and Chcmoattractant for Vascular Smooth Mus- cle Cells 显示文摘 | Yue TL Wang X Sung CP | 1994 | Circulation Research1994，75，1： | 1 |
| 10 | Assessment of hepatic fibrosis by transient elastography in patients with chronic hepatitis B显示文摘 | Zhang YG Warg BE Wang TL | 2010 | Pathol Int2010，60，28： | 1 |
| 11 | Insulinoma causing hy-poglycemia in a patient with type 2diabetes显示文摘 | Lei WY Wang TE Chen TL | 2007 | J Formos MedAssoc2007，106，5： | 1 |
| 12 | Carvedilol,a new vasodilatingβ-adrenoceptor antagonist,prevents low-density lipoprotein(LDL)-enhanced leukocyte abhesion to endothelial cells by inhibition of LDL oxidation显示文摘 | Yue TL Wang X Gu JL | 1995 | Eue Pharmacol1995，294，： | 1 |
| 13 | Cutting edge: the Etsl transcription factor is required for the development of NK T cells in mice显示文摘 | Walunas TL Wang B Wang CR | 2000 | J Immunol2000，164，6： | 1 |
| 14 | Frequent mutations of chromatin remodeling gene ARID1A in ovarian clear cell carcinoma显示文摘 | Jones S Wang TL ShihIe M | 2010 | Science2010，330，6001： | 1 |
| 15 | Sa!inomycin selectively targets 'CD 133+' cell subpopulations and decreases malignant traits in colorectal cancer lines显示文摘 | Dong Tl? Zhou HM Wang LL | 2011 | Ann Surg Oncol2011，18，6： | 1 |
| 16 | Shortened telomeres in serous tubal intraepithelial carcinoma:an early event in ovarian high-grade serous carcinogenesis显示文摘 | Kuhn E Meeker A Wang TL | 2010 | Am J Surg Pathol2010，34，6： | 1 |
| 17 | Regulation of connexin43 gene expression by cyclical mechanical stretch in neonatal rat cardiomyocytes显示文摘 | Wang TL Tseng YZ Chang H | 2000 | Biochem Biophys Res Commun2000，267，： | 1 |
| 18 | Discovery of XL335 (WAY-362450), a highly potent, selective, and orally active agonist of the famesoid X receptor (FXR) 显示文摘 | Flatt B Martin R Wang TL | 2009 | J Med Chem2009，52，： | 1 |
| 19 | Effect of frging meat emission particulate on 17, beta-estradiol 2-and 4-hudroxy lation in human lung adenocarcinoma cells显示文摘 | Wang HW Ueng TH chen TL | 2003 | J Toxicol Enoiroh Health2003，66，12： | 1 |
| 20 | MicroRNA expression and identification of putative miRNA targets in ovarian cancer显示文摘 | Dahiya N Sherman-Baust CA Wang TL Davidson B Shih IeM Zhang Y | | 0，，： | 1 |
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