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| 1 | A novel peptide, selected from phage display library of random peptides, can efficiently target into human breast cancer cell显示文摘To develop a targeting vector for breast cancer biotherapy, MDA-MB-231 cell, a human breast cancer cell line, was co-cultured with pC89 (9 aa) phage display library of random peptides. In multiple inde- pendent peptide-presenting phage screening trials, subtilisin was used as a protease to inactivate ex- tra-cellular phages. The internalized phages were collected by cell lysising and amplified in E. coli XLI-Blue. Through five rounds of selection, the peptide-presenting phages which could be internalized in MDA-MB-231 cells were isolated. A comparison was made between internalization capacities of pep- tide-presenting phages isolated from MDA-MB-231 cells and RGD-integrin binding phage by cocultur- ing them with other human tumor cell lines and normal cells. The nucleotide sequences of isolated peptide-presenting phages were then determined by DNA sequencing. To uncover whether phage coat protein or amino acid order was required for the character of the peptide to MDA-MB-231 cells, three peptides were synthesized. They are CASPSGALRSC, ASPSGALRS and CGVIFDHSVPC (the shifted sequence of CASPSGALRSC), and after coculturing them with different cell lines, their targeting ca- pacities to MDA-MB-231 cells were detected. These data suggested that the internalization process was highly selective, and capable of capturing a specific peptide from parent peptide variants. Moreover, the targeting internalization event of peptides was an amino acid sequence dependent manner. The results demonstrated the feasibility of using phage display library of random peptides to develop new targeting system for intracellular delivery of macromolecules, and the peptide we obtained might be modified as a targeting vector for breast cancer gene therapy. | DONG Jian LIU WeiQing ZHANG KeJian JIANG AiMei CHEN MingQing | 2008 | Chinese Science Bulletin2008，53，6： | 12 |
| 2 | Transcriptome analysis of purple pigment formation in Colocasia esculenta显示文摘Taro(Colocasia esculenta(L.)Schott)is an important crop in Africa,Southeast Asia,and subtropics and is used as a food and medicine.The purple color pigmentation is an appealing character in taro.We sampled taro corms of the cultivar‘Lipu Taro’at four developmental stages,including LPYS1(without purple pigment,50 days of development(DOD)),LPYS2(very few purple pigments,75 DOD),LPYS3(moderate purple pigments,115 DOD)and LPYS4(high purple pigments,205 DOD).The purpose of our study was to identify the key genes underpinning the purple pigmentation in taro based on RNA-sequencing.Through RNA-Seq,6453 differentially expressed transcripts(DETs)were identified between purple and non-purple pigmented samples.We identified 41 and 12 flavonoid and anthocyanin related DETs transcripts,respectively.These DETs were upregulated at LPYS2,LPYS3,and LPYS4 as compared to LPYS1,indicating their positive contribution to the color formation in taro.Moreover,we identified several DETs encoding for transcription factors,including MYB and bHLH,known to be major regulators of structural genes involved in the flavonoid-anthocyanin pathway.Finally,we reported several plant hormones(ethylene,auxin,gibberellin,jasmonic acid,and cytokinin)related DETs,which are predicted to play important roles in the corm coloration.Different regulation of transcripts representing the flavonoid-anthocyanin biosynthesis pathway,plant hormone transduction pathway,and transcription factors may have key roles in purple pigmentation in taro.Our findings will facilitate future research on improving the quality and appeal of taro. | FANGLIAN HE WEIQING DONG SHAOLONG WEI ZUYANG QIU JINGLI HUANG HUIPING JIANG SHIYU HUANG LILI LIU | 2021 | BIOCELL2021，45，3： | 2 |
| 3 | 4-1 Dielectronic Recombination of 36Ar15+ and 112Sn34+ at the CSRm显示文摘The total recombination rate coefficients of lithium-like 36Ar15+ ions have been measured by employing the electron-ion merged-beams technique at the heavy ion storage ring CSRm at IMP[1]. Fig. 1 (a) shows the DR spectrum for electron energy from 0 to 35 eV in the center-of-mass (c.m.) frame involving all the DR resonances associated with 2s1=2!2p1=2 and 2s1=2!2p3=2 excitations. | Huang Zhongkui Ma Xinwen Wen Weiqiang Wang Hanbing Chuai Xiaoya Dou Lijun Zhu Xiaolong Xu Xin Zhu Linfan Xu Weiqing Mao Lijun Li Jie Zhang Xiaohu Ma Xiaoming Yan Tailai Yang Jiancheng Yuan Youjin Xiao Guoqing Xia Jiawen Xie Luyou Dong Chenzhong DR collaboration | 2015 | IMP & HIRFL Annual Report2015，，1： | 0 |
| 4 | 脂肪移植后脂肪组织的血管和脂肪生成显示文摘背景脂肪移植的主要缺点是临床上不可预测,所以常会导致外科医师和患者对结果不满意。目前,移植组织血管化和重建的机制尚不明确。方法用两种不同的方法(打碎和完整保留)处理大鼠脂肪组织,以明确移植血管生成的方式。将正常大鼠和表达绿色荧光蛋白转基因大鼠的组织进行交叉移植,观察脂肪再生过程中细胞的起源。结果几乎所有新血管的CD31内皮细胞都来源于受体组织。移植组织中新的血管主要通过受体组织中的血管长入形成的,而不是移植组织的内皮细胞重组或受体组织和移植组织血管的重建形成的。血管生成主要依赖于移植的环境。移植后8周,移植的脂肪组织容量仅下降10%,在脂肪形成过程中,主要再生细胞(CD34+)大部分来自受体组织(P〈0.05)。组织的保留在脂肪打碎组中上升73%,在脂肪完整组中上升47.5%。结论脂肪移植的血管生成主要是通过传统的“血管分支”方式,受体组织起到重要的作用。移植组织的保留方式主要涉及到来源于受体组织的CD34’脂肪前体细胞。 | Ziqing Dong Zhangsong Peng Qiang Chang Weiqing Zhan Zhaowei Zeng Shengchang Zhang Feng Lu 叶美辰 高景恒 张晨 袁继龙 | 2015 | 中国美容整形外科杂志2015，26，10： | 0 |
| 5 | 4 - 9 Dielectronic Recombination Experiment of 78Kr28+ on HIRFL-CSRm显示文摘 | Huang Zhongkui Ma Xinwen Wen Weiqiang Wang Hanbing Xu Weiqing Zhu Xiaolong Gao Yong Zhao Dongmei Cheng Xiaowei Yang Xiaodong Li jie Ma Xiaoming Yan Tailai Yang Jiancheng Mao Lijun Xia Jiawen Yuan Youjin Xu Hushan Xiao Guoqing Xu Xin ZhuLinfan ShiYinglong Dong Chenzhong | 2013 | IMP & HIRFL Annual Report2013，，1： | 0 |
| 6 | Morphogenesis of Chinese Water Chestnut Pistil and Pollen Tube during Germination显示文摘With the forms of pistil stigma of ' Guiti 2' Chinese water chestnut cultivar during the pollination as the object of observation,we reveal the pollination process of Chinese water chestnut from the microscopic point of view,to provide reference for Chinese water chestnut crossbreeding. The results show that the Chinese water chestnut pistil has 2- 4 stigmas which present white filament and vascular bundle forms,and the vessels on epidermis are thick with long translucent branched hairs; after pollination,pollen grains are tightly bound on branched hairs,and after identification,the pollen tube can penetrate branched hairs,continue to grow,and transfer the genetic material in pollen to vascular bundle. | Kunpeng OU Fanglian HE Yan WANG Wen JIANG Meiping GAO Zhiqiang BI Lijuan CHEN Weiqing DONG Jie GUI | 2016 | Asian Agricultural Research2016，8，5： | 0 |
| 7 | Perioperative treatment of liver transplantation显示文摘A case of liver transplantation in the department of general surgery of the third affiliated hospital of Inner Mongolia medical school was collected and analyzed on the basis of diagnosis,physical examination and treatment.Liver transplantation was very complicated,accompanied by various postoperative complications.So this paper aims to share experience of perioperative management of liver transplantation among physicians. | Shengbin Zhang Rui Liu Shipeng Song Weiqing Wang Jin Zhao Xiaoshi Dong | 2014 | Discussion of Clinical Cases2014，1，1： | 0 |
| 8 | Strategic Investment in Transmission and Energy Storage in Electricity Markets显示文摘The variability of renewable energy and transmission congestion provide opportunities for arbitrage by merchants in deregulated electricity markets.Merchants strategically invest to maximize their profits.This paper proposes a joint investment framework for renewable energy,transmission lines,and energy storage using the Stackelberg game model.At the upper level,merchants implement investment and operation strategies for deregulated transmission and energy storage to maximize profits.At the middle level,central planners seek to maximize social welfare through investments in centralized renewable energy and energy storage.At the lower level,independent system operators jointly optimize the energy and reserve markets to minimize the total operating costs.Merchants are remunerated through financial rights,which are a settlement method based on locational marginal price.The trilevel optimization problem is reformulated as a tractable single-level one using Karush-Kuhn-Tucker(KKT)conditions and strong duality theory.The interaction between merchants and central planners is studied with an example based on the IEEE 30-bus test system.The assignment of weight coefficients to the corresponding stochastic scenarios can help merchants avoid investment risk,and their effectiveness is verified with the IEEE 118-bus test system. | Kunpeng Tian Weiqing Sun Dong Han | 2022 | Journal of Modern Power Systems and Clean Energy2022，10，1： | 0 |
| 9 | Rapamycin enhances the anti-tumor activity of cabozantinib in cMet inhibitor-resistant hepatocellular carcinoma显示文摘Cabozantinib,mainly targeting cMet and vascular endothelial growth factor receptor 2,is the second-line treatment for patients with advanced hepatocellular carcinoma(HCC).However,the lower response rate and resistance limit its enduring clinical benefit.In this study,we found that cMet-low HCC cells showed primary resistance to cMet inhibitors,and the combination of cabozantinib and mammalian target of rapamycin(mTOR)inhibitor,rapamycin,exhibited a synergistic inhibitory effect on the in vitro cell proliferation and in vivo tumor growth of these cells.Mechanically,the combination of rapamycin with cabozantinib resulted in the remarkable inhibition of AKT,extracellular signal-regulated protein kinases,mTOR,and common downstream signal molecules of receptor tyrosine kinases;decreased cyclin D1 expression;and induced cell cycle arrest.Meanwhile,rapamycin enhanced the inhibitory effects of cabozantinib on the migration and tubule formation of human umbilical vascular endothelial cells and human growth factor-induced invasion of cMet inhibitor-resistant HCC cells under hypoxia condition.These effects were further validated in xenograft models.In conclusion,our findings uncover a potential combination therapy of cabozantinib and rapamycin to combat cabozantinib-resistant HCC. | Chao Gao Shenghao Wang Weiqing Shao Yu Zhang Lu Lu Huliang Jia Kejin Zhu Jinhong Chen Qiongzhu Dong Ming Lu Wenwei Zhu Lunxiu Qin | 2022 | Frontiers of Medicine2022，16，3： | 0 |
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