|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Opiate-induced constipation related to activation of small intestine opioid μ2-receptors显示文摘AIM:To investigate the role of opioid μ-receptor subtype in opiate-induced constipation (OIC).METHODS:The effect of loperamide on intestinal transit was investigated in mice.Ileum strips were isolated from 12-wk-old male BALB/c mice for identification of isometric tension.The ileum strips were precontracted with 1 μmol/L acetylcholine (ACh).Then,decrease in muscle tone (relaxation) was characterized after cumulative administration of 0.1-10 μmol/L loperamide into the organ bath,for a concentration-dependent study.Specific blockers or antagonists were used for pretreatment to compare the changes in loperamide-induced relaxation.RESULTS:In addition to the delay in intestinal transit,loperamide produced a marked relaxation in isolated ileum precontracted with ACh,in a dose-dependent manner.This relaxation was abolished by cyprodime,a selective opioid μ-receptor antagonist,but not modified by naloxonazine at a dose sufficient to block opioid μ-1 receptors.Also,treatment with opioid μ-1 receptor agonist failed to modify the muscle tone.Moreover,the relaxation by loperamide was attenuated by glibenclamide at a dose sufficient to block ATP-sensitive K + (K ATP) channels,and by protein kinase A (PKA) inhibitor,but was enhanced by an inhibitor of phosphodiesterase for cyclic adenosine monophosphate (cAMP).CONCLUSION:Loperamide induces intestinal relaxation by activation of opioid μ-2 receptors via the cAMPPKA pathway to open K ATP channels,relates to OIC. | Wency Chen Hsien-Hui Chung Juei-Tang Cheng | 2012 | World Journal of Gastroenterology2012，18，12： | 20 |
| 2 | Identification of immunodominant linear B-cell epitopes within the major outer membrane protein of Chlamydia trachomatis显示文摘Chlamydia trachomatis 是最流行的性播送的病原体之一。Chlamydial 主要的外部膜蛋白质(MOMP ) 能在鼠科的模型导致强壮的细胞、体液的有免疫力的回答并且被认为是一个潜在的疫苗的候选人。在这份报告, MOMP 的氨基酸顺序用帮助计算机的技术被分析扫描 B 房间 epitopes,和三可能的线性 B 房间 epitopes 肽(VLKTDVNKE, TKDASIDYHE, TRLIDERAAH ) 与高预言的 antigenicity 和高度,保存被调查。为各潜在的 epitope 编码区域的 DNA 被克隆进 pET32a (+) 并且在 Escherichia coli 表示了为 Trx-His-tag 熔化蛋白质。熔化蛋白质被 Ni-NTA agarose 祷告净化并且由 SDS 页和西方的污点分析列在后面。我们与这三熔化蛋白质使老鼠免疫。从使免疫的老鼠包含 anti-epitope 抗体的 sera 能认出 C。在 ELISA 的 trachomatis serovars D 和 E。这些熔化蛋白质的 Antisera 在 serovar E 的侵略上显示了禁止的效果由在 vitro 中立化试金。另外,浆液从恢复健康的 C 取样。感染 trachomatis 的病人与由西方的污点试金的 epitope 熔化蛋白质是反应的。我们的结果证明生物信息的分析选择的 epitope 序列是高度保存的 C。trachomatis MOMP B 房间 epitopes,并且能是子单元疫苗的发展的好候选人,它能在临床的诊断被使用。 | Shanli Zhu Jun Chen Meixia Zheng Wenci Gong Xiangyang Xue Wenshu Li Lifang Zhang | 2010 | Acta Biochimica et Biophysica Sinica2010，42，11： | 11 |
| 3 | Expression of pituitary tumor transforming gene in human gastric carcinoma显示文摘 | WenCY NakayamaT WangAP NakashimaM DingYT ItoM IshibashiH MatsuuM ShichijoK | 2004 | 第四军医大学学报2004，25，5： | 6 |
| 4 | Stable carbon isotopes in the shell of Corbicula fluminea (Müller 1774): Implications for understanding environmental changes in drainage basins显示文摘Dissolved inorganic carbon (DIC) is a primary constituent of the aquatic geochemistry of terrestrial ecosystems. Changes in DIC concentration and its isotopic composition are closely associated with environmental changes in the drainage basin. Thus, a better understanding of DIC may lead to improved characterization of environmental changes. Corbicula fluminea (Müller 1774), a bivalve native to southeast Asia and a dominant invasive species in many aquatic environments around the world, has the poten-tial to record DIC information, particularly because of its sequential skeletal deposition. Analysis of the stable carbon isotope composition (δ 13C) of the shell and the aquatic DIC in the Xijiang River drainage basins in southern and western China allowed us to determine that changes in δ 13C in the skeleton significantly correlated with those of DIC (P < 0.01, r = 0.63). These results indicate that the isotopic signature of the shell reflects that δ 13C of the DIC in ambient water. The temporal resolution was at the seasonal scale. Metabolic effect does not change the co-variation, even though it makes the shell δ 13C 1.4‰ lower statistically than that of DIC. δ 13C of DIC in creeks of some watershed areas may experience large temporal changes characterization of these changes requires high sampling resolution of the shell. This problem makes the clams in these areas less preferable for reconstruc-tion of environmental changes. The present findings provide the basis for recovering paleoenvironmental changes and carbon cycles in a watershed through analysis of fossil shells of C. fluminea, which are common in some lacustrine and/or riverine sedi-ment sequences. | ZHOU Hui LIU CongQiang JIANG Qian JIANG Wei ZHAO YanLong DING WenCi YAN Hui | 2010 | Chinese Science Bulletin2010，55，36： | 2 |
| 5 | A multi-epitope vaccine based on Chlamydia trachomatis major outer membrane protein induces specific immunity in mice显示文摘我们评估了包括主要的外部膜蛋白质(MOMP ) 的一支候选人疫苗的 immunogenicity 和功效 multi-epitopeof Chlamydia trachomatis。从包含多重 T 房间和 B 房间 epitopes 的 MOMP 导出的 multi-epitope 的短基因是人工地,包含 codon 的 synthesized.The recombinant plasmid pET32a (+) 优化了 MOMP multi-epitope 基因被构造。在 Escherichia coli 的 fusionprotein Trx-His-MOMP multi-epitope 的表示被钠 dodecyl sulfatepolyacrylamide 胶化电气泳动和西方的污点分析证实。Balb/c 老鼠是 inoculatedwith 皮下地,三与 2 星期的间隔预定的净化的熔化蛋白质。结果出现了 MOMP multi-epitopeelicited 不仅对 C 的强壮的体液的有免疫力的回答。由显著地产生特定的抗体(IgG1 和 IgG2a ) 的高水平的 trachomatis,而且由 inducingrobust 的细胞的有免疫力的回答在老鼠的细胞毒素的 T 淋巴细胞回答。而且, MOMP multi-epitope 实质地告知 IFN- 的分泌物,表明这支疫苗能导致强壮的 Th1 回答。最后,老鼠与 C 的 MOMP multi-epitope displayeda 减小种牛痘。在 chlamydial 挑战和感染的 C 的一个加速的清理之上流的 trachomatis。trachomatis。在结论, MOMP multi-epitope 疫苗可以对 C 为有效预防 andtherapeutic 疫苗的发展有潜力。trachomatis 感染。 | Jianxin Tu B ailong Hou Bingbing Wang Xiaoyun Lin Wenci Gong Haiyan Dong Shanli Zhu Shao Chen Xiangyang Xue Kong-Nan Zhao Lifang Zhang | 2014 | Acta Biochimica et Biophysica Sinica2014，46，5： | 1 |
| 6 | Ageneralizedmethodforpredicting the minimum fluidization velocity显示文摘 | WenCY YuH | 1966 | AIChE Journal1966，，12： | 1 |
| 7 | On the convergence property of the DFP algorithm显示文摘 | Dingguo Pu Wenci Yu | 1990 | Annals of Operations Research1990，，1： | 1 |
| 8 | Scheduling one batch processor subject to job release dates 显示文摘 | Zhaohui Liu Wenci Yu | 2000 | Discrete Applied Mathematics2000，105，： | 1 |
| 9 | Collagen Triple Helix Repeat Containing 1 Promotes Melanoma Cell Adhesion and Survival显示文摘 | Wency I Olivier WL Tang L | 2011 | J Cutan Med Surg2011，15，2： | 1 |
| 10 | Scheduling one batch processor subject to job release dates显示文摘 | LIU Zhaohui YU Wenci | 2000 | Disc Appl Math2000，105，123： | 1 |
| 11 | A comparison of surgical outcomes between laparoscopic and open myomectomy in Southern Taiwan显示文摘 | Chi-Chang Chang Wency Chen | 2012 | International Journal of Gynecology and Obstetrics2012，，119： | 1 |
| 12 | On the conuergence of the DFP algorithm (in Chines)显示文摘 | Pu Dingguo Yu Wenci | 1988 | Journal of Qufu Normal University1988，14，3： | 1 |
| 13 | A comparison of surgi- cal outcomes between laparoscopic and open myomectomy in Southern Taiwan 显示文摘 | CHI-CHANG CHANG WENCY CHEN | 2012 | International Journal of Gynecology and Ob- stetrics2012，119，2： | 1 |
| 14 | On the convergence property of the DFP algorithm显示文摘 | Dingguo Pu Wenci Yu | 1990 | Annals of Operations Research1990，，1： | 1 |
| 15 | Scheduling one batch processor subjecl to job release dales 显示文摘 | Zhaohui Liu Wenci Yu | 2000 | Discrete Applied Mathematics2000，105，1： | 1 |
| 16 | On the convergence property of the DFP algorithm显示文摘 | Dingguo Pu Wenci Yu | 1990 | Annals of Operations Research1990，，1： | 1 |
| 17 | One-stepsensitivedetectionofsalmonellatyphimuriumbycouplingmagneticcaptureandfluorescenceidentificationwithfunctionalnanospheres显示文摘 | WenCY HuJ ZhangZL etal | 2013 | AnalChem2013，85，2： | 1 |
| 18 | Designandmodelingofelectromagneticactuatorinmems-basedvalvelessimpedancepump显示文摘 | ChangH T LeeCY WenCY | 2007 | MicrosystTechnol2007，13，： | 1 |
| 19 | On the convergence property of the DFP algorithm显示文摘 | Dingguo Pu Wenci Yu | 1990 | Annals of Operations Research1990，，1： | 1 |
| 20 | A comparision of surgical outcomes between laparoscopic and open myomectomy in Southern Taiwan 显示文摘 | Chi-Chang Chang Wency Chen | 2012 | International Journal of Gynecology and Obstetrics2012，119，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员