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| 1 | Altered vasoactive intestinal peptides expression in irritable bowel syndrome patients and rats with trinitrobenzene sulfonic acid-induced colitis显示文摘AIM: To investigate the vasoactive intestinal peptides(VIP) expression in irritable bowel syndrome(IBS) and trinitrobenzene sulfonic acid(TNBS) induced colitis.METHODS: The VIP gene expression and protein plasma levels were measured in adult participants(45.8% male) who met Rome Ⅲ criteria for IBS for longer than 6 mo and in a rat model of colitis as induced by TNBS.Plasma and colons were collected from naive and inflamed rats.Markers assessing inflammation(i.e.,weight changes and myeloperoxidase levels) were assessed on days 2,7,14 and 28 and compared to controls.Visceral hypersensitivity of the rats was assessed with colo-rectal distension and mechanical threshold testing on hind paws.IBS patients(n = 12) were age,gender,race,and BMI-matched with healthy controls(n = 12).Peripheral whole blood and plasma from fasting participants was collected and VIP plasma levels were assayed using a VIP peptide-enzyme immunoassay.Human gene expression of VIP was analyzed using a custom PCR array.RESULTS: TNBS induced colitis in the rats was confirmed with weight loss(13.7 ± 3.2 g) and increased myeloperoxidase activity.Visceral hypersensitivity tocolo-rectal distension was increased in TNBS treated rats up to 21 d and resolved by day 28.Somatic hypersensitivity was also increased up to 14 d post TNBS induction of colitis.The expression of an inflammatory marker myeloperoxidase was significantly elevated in the intracellular granules of neutrophils in rat models following TNBS treatment compared to naive rats.This confirmed the induction of inflammation in rats following TNBS treatment.VIP plasma concentration was significantly increased in rats following TNBS treatment as compared to naive animals(P < 0.05).Likewise,the VIP gene expression from peripheral whole blood was significantly upregulated by 2.91-fold in IBS patients when compared to controls(P < 0.00001; 95%CI).VIP plasma protein was not significantly different when compared with controls(P = 0.193).CONCLUSION: Alterations in VIP expression may play a role in IBS.Therefore,a better understanding of the physiology of VIP could lead to new therapeutics. | Arseima Y Del Valle-Pinero LeeAnne B Sherwin Ethan M Anderson Robert M Caudle Wendy A Henderson | 2015 | World Journal of Gastroenterology2015，21，1： | 7 |
| 2 | 在有煽动性、非煽动性的腹的疼痛显型的孩子的 interleukin-6 和桅杆房间的反的关系显示文摘 AIM: To investigate interleukin-6 (IL-6), mast cells, enterochromaffin cells, 5-hydroxytryptamine, and substance P in the gastrointestinal mucosa of children with abdominal pain. METHODS: Formalin-fixed paraffin-embedded gas-trointestinal biopsy blocks from patients (n = 48) with non-inflammatory bowel disease (irritable bowel syndrome and functional abdominal pain) and inflammatory bowel disease were sectioned and stained for IL-6, mast cells, enterochromaffin cells, 5-hydroxytryptamine, and substance P. All children had chronic abdominal pain as part of their presenting symptoms. Biopsy phenotype was confirmed by a pathologist, blinded to patient information. Descriptive statistics, chi-square, and independent sample t tests were used to compare differences between the inflammatory and non-inflammatory groups. RESULTS: The cohort (n = 48), mean age 11.9 years (SD = 2.9), 54.2% females, 90% Caucasian, was comprised of a non-inflammatory (n = 26) and an inflammatory (n = 22) phenotype. There was a significant negative correlation between substance P expression and mast cell count (P = 0.05, r = -0.373). Substance P was found to be expressed more often in female patient biopsies and more intensely in the upper gastrointestinal mucosa as compared to the lower mucosa. There were significantly increased gastrointestinal mucosal immunoreactivity to IL-6 (P = 0.004) in the inflammatory phenotype compared to non-inflammatory. Additionally, we found significantly increased mast cells (P = 0.049) in the mucosa of the non-inflammatory phenotype compared to the inflammatory group. This difference was particularly noted in the lower colon biopsies. CONCLUSION: The findings of this study yield preliminary evidence in identifying biomarkers of undiagnosed abdominal pain in children and may suggest candidate genes for future evaluation. | Wendy A Henderson Ravi Shankar Tara J Taylor Arseima Y Del Valle-Pinero David E Kleiner Kevin H Kim Nader N Youssef | 2012 | World Journal of Gastrointestinal Pathophysiology2012，3，6： | 2 |
| 3 | CD4+ CD25high Foxp3 6 regulatory T cells in myelo- dysplastic syndrome (MDS)显示文摘 | Shahram Y Kordasti Wendy Ingram Janet Hayden | 2007 | Blood2007，110，3： | 1 |
| 4 | Detecting site-Specific physicochemical selective pressures:applications to the class I HLA of the human major histocompatibility complex and the SRK of the plant sporophytic self-incompatibility system显示文摘 | Raazesh S Wendy S W Krithika Y | 2005 | J Mol Evol2005，60，： | 1 |
| 5 | Angiotensin I con- verting enzyme inhibitory peptides from in vitro pepsin- pancreatin digestion of soy protein 显示文摘 | Wendy M Y Lo Eunice C Y Li-Chan | 2005 | Journal of Agri- cultural and Food Chemistry2005，53，9： | 1 |
| 6 | Second law analysis of the transcritical CO2 refrigeration cycle显示文摘 | DAVID S K T WENDY W Y | 2004 | Energy Conversion and Management2004，45，： | 1 |
| 7 | Supply net- work synthesis on rubber seed oil utilisation as potential biofuel feedstock 显示文摘 | WENDY P Q N HON L L SUZANA Y | 2013 | Energy2013，55，： | 1 |
| 8 | Examining the effects of metacognitive strategy instruction on ESL group discussions : a synthesis of approaches 显示文摘 | Wendy Y K L | 2009 | Language Teaching Research2009，13，2： | 1 |
| 9 | Global stability of a competition model with periodic coefficients and time delays显示文摘 | Wang Wendi Chen L s Lu z y | 1995 | Canadian Appl Math Quart1995，13，3： | 1 |
| 10 | Does early pulmonary rehabilita- tion reduce acute health - care utilization in COPD patients admit- ted with an exacerbation? A randomized controlled study显示文摘 | Tam E Pam Y Wendy F | 2009 | Re spirology2009，14，： | 1 |
| 11 | Comcumption Preferences and Environmental Externalities: A hedonic Analysis of the Housing MarKet in Guangzhou 显示文摘 | C Y Jim Wendy Y Chen | 2007 | Geoforum2007，，2： | 1 |
| 12 | Placental abruption 显示文摘 | Katherine H Jerone Y Wendy HF | 2002 | Obstet Gynecol2002，57，5： | 1 |
| 13 | Placental abruption显示文摘 | Katherine H Jerone Y Wendy HF | 2002 | Obstet Gynecol2002，57，5： | 1 |
| 14 | Phenotypic spectrum of polycystic ovary syndrome:Clinical and biochemical characterization of the three major clinical subgroups 显示文摘 | Wendy Y Chang M D Eric S | 2005 | Fertil Steril2005，3，6： | 1 |
| 15 | Assessing the toxicity of ingested Taihu Lake water on mice via hepatic histopathology and matrix metalloproteinase expression显示文摘 | Zong Y Z Wendi Q Shupei C | 2011 | Ecotoxicology2011，20，5： | 1 |
| 16 | Does early pulmonary rehabilitationreduce acute health-care utilization in COPD patients admitted with an exacerbation?A randomized controlled study显示文摘 | Tam E Pam Y Wendy F et a/ | 2009 | Respirology2009，14，2： | 1 |
| 17 | Detecting site specific physicochemical selective pressures: applications to the Class I HLA of the human major histocompatibility complex and the SRK of he plant sporophyic self-incompatibility system显示文摘 | Raazesh S Wendy S W Krithika Y | 2005 | J MolEvol2005，60，3： | 1 |
| 18 | Ecosystem services and valuation of urban forests in China显示文摘 | Jim C Y Wendy Y C | 2009 | Cities2009，26，4： | 1 |
| 19 | Pattern and Divergence of Tree Communities in Taipei' s Main Urban Green Spaces显示文摘 | Jim C Y Chen Wendy Y | 2008 | Landscape and Urban Planning2008，84，3： | 1 |
| 20 | Ecosystem services and valuation of urban forests in China显示文摘 | Jim C Wendy Y | 2009 | Cities2009，26，4： | 1 |
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