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| 1 | Liuwei Dihuang pill suppresses metastasis by regulating the wnt pathway and disrupting β-catenin/T cell factor interactions in a murine model of triple-negative breast cancer显示文摘OBJECTIVE:To investigate if the Liuwei Dihuang pill(LWDHP)can inhibit metastasis to the liver and lungs in mice bearing triple-negative breast cancer(TNBC),and the molecular mechanism underpinning this action.METHODS:Ninety-nine TNBC bearing-mice were distributed randomly to five groups:control(Con),paclitaxel(PTX),low-dose LWDHP(LLP,2.3 g·kg^-1·d^-1),middle-dose LWDHP(MLP,4.6 g·kg^-1·d^-1)and high-dose LWDHP(HLP,9.2 g·kg^-1·d^-1).The LWDHP were administered(p.o.)to the agonal stage.The morphology of BC cells was observed by hematoxylin&eosin staining.Expression of axin-2,β-catenin,T cell factor(TCF),cyclin-D1 and vascular endothelial growth factor(VEGF)was detected by western blotting or immunofluorescence.β-catenin/TCF-1 interaction was measured using a co-immunoprecipitation assay.RESULTS:After LWDHP treatment,metastasis of BC cells to the lungs and liver was inhibited,expression of axin-2 was increased,expression of TCF-1,β-catenin,cyclin-D1 and VEGF was decreased,andβ-catenin/TCF-1 interaction was disrupted.CONCLUSION:The LWDHP could inhibit metastasis of BC cells to the liver and lungs.The molecular mechanism underlying this action may be regulation of protein expression andβ-catenin/TCF-1 interactions in the Wnt pathway. | Zheng Lixiang Zheng Qing Yu Zhipeng Wang Jian Ren Xiaoying Gong Yan Yang Xue Hunag Ping Weng Meizhi Liu Hongning Liu Haizhou | 2019 | Journal of Traditional Chinese Medicine2019，39，6： | 9 |
| 2 | Thermostability of Subtilisin Nattokinase Obtained by Site-Directed Mutagenesis显示文摘To study the thermostability of Nattokinase(subtilisin NAT,NK),three double mutant plasmids(pET-28a-NKG61C/S98C,pET-28a-NKT22C/S87C,pET-28a-NKS24C/S87C)were constructed by site-directed mutagenesis.Target enzymes were detected using SDS-PAGE and disulfide bond formation was detected using Western blotting analysis.Thermostability was tested by rates of inactivation at certain temperature.The results showed that disulfide bond was not formed within two cysteines and the thermostability of three double mutants was not increased compared with the wild-type NK.The thermostability of NK performed in Ca2+was stronger than in ethylenediaminetetraacetic acid(EDTA).But when the temperature reached 62℃,the enzymes rapidly denatured and inactivated even in the presence of Ca2+.Although the thermostability of mutants was not increased,this study shows a tendency of improving thermostability of NK in protein engineering. | WENG Meizhi DENG Xiongwei WU Jieyuan ZOU Guolin | 2014 | Wuhan University Journal of Natural Sciences2014，19，3： | 6 |
| 3 | Contents Variation Analysis ofγ-Amino Butyric Acid in Semen sojae praeparatum Fermentation Using Online Pre-Column Derivatization-HPLC显示文摘This paper reported the contents variation analysis ofγ-amino butyric acid(GABA)in Semen sojae praeparatum(SSP)which is a famous traditional Chinese medicine.High performance liquid chromatography(HPLC)was used and GABA was derivatized by online pre-column derivatization with o-phthalaldehyde(OPA).To validate this method,the precision,stability,repeatability and recovery were discussed.In the concentration range from 0.0125 to 0.400 mg/m L,the calibration curve for GABA was linear and the regression equation was obtained with correlation coefficient(R2)of 0.9999.Relatively high levels of GABA exist in SSP and the content changes of GABA at different time points during the fermenting process were detected.At the'yellow cladding'stage,GABA level was very low or even undetectable;the'secondary fermentation'stage witnessed a rapid increase of GABA content to 1.39-5.52 mg/g,which remained stable after 18 days of'secondary fermentation'.This study demonstrated that GABA was generated at the'secondary fermentation'stage,revealing the significance and rationality of the'secondary fermentation'stage in the fermenting process of SSP.On the other hand,it suggested the downside of taking soy isoflavones as the only measurement in existing quality assessment and optimization approach for the fermenting process of SSP. | WENG Meizhi HE Jing REN Jiaxiu LIANG Yonghong CHEN Qingfeng SU Mingsheng WANG Liyuan XIE Xiaomei | 2021 | Wuhan University Journal of Natural Sciences2021，26，1： | 2 |
| 4 | Risk Management Strategy for Agricultural Operation Subjects in Simple Scale Expansion Mode显示文摘Agricultural operation subject is a main body of the market economy. At present,the simple scale expansion mode is a major mode of agricultural operation in China. According to some hypothesis,scale expansion will inevitably bring high income,but such hypothesis has serious defects. The operation mechanism of the simple scale expansion mode includes farmer operation mechanism,professional farmer operation mechanism,and ' company + farmers' operation mechanism. In the production and operation,they will be faced with different natural risks,technical risks,market risks and policy risks. Besides,their risk control ability is also varied. Therefore,it is required to take different agricultural risk management strategies. The ' company + farmers' production and operation subjects have the highest ability of risk management. Other subjects should learn their experience. | Meizhi FAN Wenxian WENG | 2017 | Asian Agricultural Research2017，9，7： | 0 |
| 5 | Prevention of Agricultural Market Risks by Agricultural Production Entities in Hubei Province显示文摘Different agricultural production and operation entities face the same market risks.Since there is a huge gap in market risk prevention ability between agricultural market operation entities,the gap of risk prevention effect is huge.Using statistical analysis and comparative analysis methods,this paper analyzed characteristics and influencing factors of agricultural market risks and pointed out characteristics of agricultural market and special characteristics of agricultural production and operation entities in Hubei Province.Based on SWOT analysis on market risk prevention ability of agricultural production and operation entities in Hubei Province,it came up with three recommendations,including upgrading agricultural production and operation entities,enlarging the production and operation scale,and strengthening the ability of balancing the market demand and supply and bargaining power and pricing power. | Wenxian WENG Meizhi FAN | 2017 | Asian Agricultural Research2017，9，8： | 0 |
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