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| 1 | Identification of immunoreactive proteins of Brucella melitensis by immunoproteomics显示文摘Infection with Brucella causes brucellosis, a chronic disease in humans, which induces abortion and sterility in livestock. Among the different Brucella species, Brucella melitensis is considered the most virulent and is the predominant species associated with outbreaks in China. To date, no safe human vaccine is available against Brucella infection. The currently used live vaccines against Brucella in livestock induce antibodies that interfere with the diagnosis of field infection in vaccinated animals, which is harmful to eradication programs. However, there is as yet no complete profile of immunogenic proteins of B. melitensis. Towards the development of a safer, equally efficacious, and field infection-distinguishable vaccine, we used immunoproteomics to identify novel candidate immunogenic proteins from B. melitensis M5. Eighty-eight immunoreactive protein spots from B. melitensis M5 were identified by Western blotting and were assigned to sixty-one proteins by mass spectrometry, including many new immunoreactive proteins such as elongation factor G, F0F1 ATP synthase subunit beta, and OMP1. These provide many candidate immunoreactive proteins for vaccine development. | ZHAO ZhongPeng YAN Fang JI WenHui LUO DeYan LIU Xin XING Li DUAN YueQiang YANG PengHui SHI XiuMin LI Zhong WANG XiLiang | 2011 | Science China(Life Sciences)2011，54，9： | 12 |
| 2 | Photosensitive hydrogels: from structure, mechanisms, design to bioapplications显示文摘Hydrogel is a smart material with a three-dimensional network structure and has been widely used in various fields due to its good biodegradability, biocompatibility, and modification. Photosensitive hydrogel is a smart hydrogel, and its amenability to remote, precise control, and flexible and convenient regulation of stimulating factors make it an ideal candidate for use in fields such as biological materials, drug carriers, and sensors. In this review, we discuss the structure, mechanisms, design principles,and bioapplications of photosensitive hydrogels as developed in recent years. Finally, their potential for development and potential future challenges are outlined. | Wenhui Ji Qiong Wu Xisi Han Wei Zhang Wei Wei Liang Chen Lin Li Wei Huang | 2020 | Science China(Life Sciences)2020，63，12： | 4 |
| 3 | Controlled release of hydrogen by implantation of magnesium induces P53-mediated tumor cells apoptosis显示文摘Hydrogen has been used to suppress tumor growth with considerable efficacy.Inhalation of hydrogen gas and oral ingestion of hydrogen-rich saline are two common systemic routes of hydrogen administration.We have developed a topical delivery method of hydrogen at targeted sites through the degradation of magnesium-based biomaterials.However,the underlying mechanism of hydrogen’s role in cancer treatment remains ambiguous.Here,we investigate the mechanism of tumor cell apoptosis triggered by the hydrogen released from magnesium-based biomaterials.We find that the localized release of hydrogen increases the expression level of P53 tumor suppressor proteins,as demonstrated by the in vitro RNA sequencing and protein expression analysis.Then,the P53 proteins disrupt the membrane potential of mitochondria,activate autophagy,suppress the reactive oxygen species in cancer cells,and finally result in tumor suppression.The anti-tumor efficacy of magnesium-based biomaterials is further validated in vivo by inserting magnesium wire into the subcutaneous tumor in a mouse.We also discovered that the minimal hydrogen concentration from magnesium wires to trigger substantial tumor apoptosis is 91.2μL/mm^(3)per day,which is much lower than that required for hydrogen inhalation.Taken together,these findings reveal the release of H2 from magnesium-based biomaterial exerts its anti-tumoral activity by activating the P53-mediated lysosome-mitochondria apoptosis signaling pathway,which strengthens the therapeutic potential of this biomaterial as localized anti-tumor treatment. | Rui Zan Hao Wang Weijie Cai Jiahua Ni Berengere J.C.Luthringer-Feyerabend Wenhui Wang Hongzhou Peng Weiping Ji Jun Yan Jiazeng Xia Yang Song Xiaonong Zhang | 2022 | Bioactive Materials2022，7，3： | 3 |
| 4 | Improving the mechanical property of dissimilar Al/Mg hybrid friction stir welding joint by PIO-ANN显示文摘Ultrasonic-stationary shoulder assisted friction stir welding(U-SSFSW)is a novel hybrid welding technique,which reveals promising prospect in joining Al/Mg dissimilar alloys.A thorough understanding of U-SSFSW process is imperative for the further application of this technique.Pigeon-inspired optimization(PIO)is a swarm intelligent optimization algorithm and is proposed in mathematical modeling and process optimization by artificial intelligence.In this study,PIO optimized artificial neural network(PIOANN)was firstly established to acquire the relationships between the inputs and output of the Al/Mg welding process by U-SSFSW technique.A reliable PIO-ANN was achieved and the joint with a tensile strength of 161 MPa was acquired under the PIO optimized parameters.This tensile strength is higher than any ever-reported results with the similar welding condition.The joint formation,microstructure,microhardness and fracture behaviors were systemically investigated based on the reported studies and the confirmation experiment of this study to explore the enhancing mechanism of U-SSFSW Al/Mg joint. | Wei Hu Zhongwei Ma Shude Ji Qi Song Mingfei Chen Wenhui Jiang | 2020 | Journal of Materials Science & Technology2020，52，18： | 3 |
| 5 | Flue Gas Water Recovery by Indirect Cooling Technology for Large-Scale Applications: A Review显示文摘With social development and economic enhancement,energy is facing significant worldwide demand,and fossil fuels are the prime energy sources for various energy systems over past decades.Furthermore,among fuel-consumed applications,power plants are the primary source of energy consumption.There is a lot of waste heat and steam accompanied by the latent heat produced in the exhaust flue gas.Therefore,the latent heat recovery from the flue gas plays an important role in increasing the efficiency of the system and saving water.To recover the heat and mass in power plants,three primary methods are proposed to condense the vapor based on previous studies:(1)flue gas condensation technology,(2)liquid desiccant-based dehydration(LDD)technology and(3)membrane technology.This paper mainly reviews and summaries the indirect cooling technology in flue gas condensation technology.The numerical simulation and theory of flue gas condensation are introduced.Different heat exchanger types and conducted experiments are also summarized.The performance of the indirect cooling technology is affected not only by its own configuration and design but also by the flue gas inlet temperature,velocity,water vapor mass fraction,etc.The major concerns and outlook of practical applications for further study are attributed to the heat exchanger size and cost,acid corrosion,ash accumulation in flue gas,etc. | ZHONG Wei JI Wenhui CAO Xiaoling YUAN Yanping | 2020 | Journal of Thermal Science2020，29，5： | 3 |
| 6 | SRSF1 plays a critical role in invariant natural killer T cell development and function显示文摘Invariant natural killer T(iNKT)cells are highly conserved innate-like T lymphocytes that originate from CD4^(+)CD8^(+)double-positive(DP)thymocytes.Here,we report that serine/arginine splicing factor 1(SRSF1)intrinsically regulates iNKT cell development by directly targeting Myb and balancing the abundance of short and long isoforms.Conditional ablation of SRSF1 in DP cells led to a substantially diminished iNKT cell pool due to defects in proliferation,survival,and TCRαrearrangement.The transition from stage 0 to stage 1 of iNKT cells was substantially blocked,and the iNKT2 subset was notably diminished in SRSF1-deficient mice.SRSF1 deficiency resulted in aberrant expression of a series of regulators that are tightly correlated with iNKT cell development and iNKT2 differentiation,including Myb,PLZF,Gata3,ICOS,and CD5.In particular,we found that SRSF1 directly binds and regulates pre-mRNA alternative splicing of Myb and that the expression of the short isoform of Myb is substantially reduced in SRSF1-deficient DP and iNKT cells.Strikingly,ectopic expression of the Myb short isoform partially rectified the defects caused by ablation of SRSF1.Furthermore,we confirmed that the SRSF1-deficient mice exhibited resistance to acute liver injury uponα-GalCer and Con A induction.Our findings thus uncovered a previously unknown role of SRSF1 as an essential post-transcriptional regulator in iNKT cell development and functional differentiation,providing new clinical insights into iNKT-correlated disease. | Jingjing Liu Menghao You Yingpeng Yao Ce Ji Zhao Wang Fang Wang Di Wang Zhihong Qi Guotao Yu Zhen Sun Wenhui Guo Juanjuan Liu Shumin Li Yipeng Jin Tianyan Zhao Hai-Hui Xue Yuanchao Xue Shuyang Yu | 2021 | Cellular & Molecular Immunology2021，18，11： | 3 |
| 7 | Growth of atomically thick transition metal sulfide films on graphene/6H-SiC(0001) by molecular beam epitaxy显示文摘我们报导原子地厚的 NbS 2, TaS 2, 和 FeS 的生长和描述在底层与单层或 bilayer 终止了的 6H-SiC (0001 ) 上拍摄取向附生的 graphene。水晶和电子结构被扫描通道显微镜学和思考学习高精力的电子衍射。NbS 2 单层完全在 2H 结构,当 TaS 2 单层包含 1T 和 2H 结构时。费用密度波浪在所有阶段被观察。为 FeS 电影,四角形的结构与六角形的和没有超导性共存被观察。 | Haicheng Lin Wantong Huang Kun Zhao Chaosheng Lian Wenhui Duan Xi Chen Shuai-Hua Ji | 2018 | Nano Research2018，11，9： | 2 |
| 8 | Expression of PTEN and mTOR in pancreatic neuroendocrine tumors显示文摘 | Xu Han Yuan Ji Jing Zhao Xuefeng Xu Wenhui Lou | 2013 | Tumor Biology2013，，5： | 1 |
| 9 | Quantitive analysis of snowmelt erosion about cold highway slopes显示文摘 | Ji Guanghong Zhu Wenhui Gao Yu Li Chi | 2012 | Advances in Biomedical Engineering2012，6，： | 1 |
| 10 | Learning Bayesian network parameters under incomplete data with domain knowledge显示文摘 | Wenhui Liao Qiang Ji | 2009 | Pattern Recognition2009，，42： | 1 |
| 11 | Optimization design of fertilizer apparatus owned arc gears based on discrete element method显示文摘To improve the uniformity of the flow when fertilizer apparatus discharges fertilizer,a kind of fertilizer apparatus owned arc gears was designed.Also,the design and working principle of its general structure and key components were analyzed theoretically.Aiming at exploring the influence of arc structural parameters to the performance of arc gears discharging fertilizer,DEM simulation was used in this study.Fertilizer apparatus owned arc gears was taken as the research object to analyze the influence of two key components including the arc radius of gears’concave-grooves and the minimal length between two mutual meshing gears.The variation coefficient of fertilizer discharging amount stability and the linear determined coefficient of fertilizer discharging amount in unit time as evaluation index were selected.Two factors and five levels quadratic rotation orthogonal experiment was applied,and Design-Expert 8.0 software was used to achieve data processing and analysis.The results of experiment indicated that the arc radius of gears’concave-grooves had significant effect on the linear determined coefficient of fertilizer discharging amount in unit time,and the minimal length between two mutual meshing gears had significant effect on the variation coefficient of fertilizer discharging amount stability.The optimum structural parameters were 8.54 mm as the arc radius of gears’concave-grooves and 5.22 mm as the minimal length between two mutual meshing gears.Upon this circumstance,the variation coefficient was 0.28 and determination coefficient was 0.9972.The optimum apparatus was selected to conduct the bench test and field simulation test.The results indicated that the variation coefficient is 0.27,and the linear determined coefficient of is 0.9980.The results of simulation experiment were anastomotic basically with the real result,which showed that DEM simulation technology was usable to the experiment of testing fertilizer discharging.The results of this research can provide a reference to the improving of performance in discharging fertilizer. | Guoqiang Dun Zhiyong Gao Yuxuan Liu Wenyi Ji Ning Mao Xingpeng Wu Wenhui Liu | 2021 | International Journal of Agricultural and Biological Engineering2021，14，2： | 1 |
| 12 | Wearable Sweat Biosensors Refresh Personalized Health/Medical Diagnostics显示文摘Sweat contains a broad range of critical biomarkers including ions,small molecules,and macromolecules that may indirectly or directly reflect the health status of the human body and thereby help track disease progression.Wearable sweat biosensors enable the collection and analysis of sweat in situ,achieving real-time,continuous,and noninvasive monitoring of human biochemical parameters at the molecular level.This review summarizes the physiological/pathological information of sweat and wearable sweat biosensors.First,the production of sweat pertaining to various electrolytes,metabolites,and proteins is described.Then,the compositions of the wearable sweat biosensors are summarized,and the design of each subsystem is introduced in detail.The latest applications of wearable sweat biosensors for outdoor,hospital,and family monitoring are highlighted.Finally,the review provides a summary and an outlook on the future developments and challenges of wearable sweat biosensors with the aim of advancing the field of wearable sweat monitoring technology. | Wenhui Ji Jingyu Zhu Wanxia Wu Nanxiang Wang Jiqing Wang Jiansheng Wu Qiong Wu Xuewen Wang Changmin Yu Gaofeng Wei Lin Li Fengwei Huo | 2021 | Research2021，，1： | 1 |
| 13 | Quantitive anal-ysis of snowmelt erosion about cold highway slopes 显示文摘 | Ji Guanghong Zhu Wenhui Gao Yu | 2012 | Ad-vances in Biomedical Engineering2012，6，： | 1 |
| 14 | Wearable Sweat Biosensors Refresh Personalized Health/Medical Diagnostics显示文摘Sweat contains a broad range of critical biomarkers including ions,small molecules,and macromolecules that may indirectly or directly reflect the health status of the human body and thereby help track disease progression.Wearable sweat biosensors enable the collection and analysis of sweat in situ,achieving real-time,continuous,and noninvasive monitoring of human biochemical parameters at the molecular level.This review summarizes the physiological/pathological information of sweat and wearable sweat biosensors.First,the production of sweat pertaining to various electrolytes,metabolites,and proteins is described.Then,the compositions of the wearable sweat biosensors are summarized,and the design of each subsystem is introduced in detail.The latest applications of wearable sweat biosensors for outdoor,hospital,and family monitoring are highlighted.Finally,the review provides a summary and an outlook on the future developments and challenges of wearable sweat biosensors with the aim of advancing the field of wearable sweat monitoring technology. | Wenhui Ji Jingyu Zhu Wanxia Wu Nanxiang Wang Jiqing Wang Jiansheng Wu Qiong Wu Xuewen Wang Changmin Yu Gaofeng Wei Lin Li Fengwei Huo | 2022 | Research2022，，1： | 1 |
| 15 | The effect of Pr co-dopant on the performance of solid oxide fuel ceils with Sin-doped eeria electrolyte 显示文摘 | Ji Yuan Liu Jiang He Tianmin Wang Jinxia Su Wenhui | 2005 | Journal of Alloys and Compounds2005，389，12： | 1 |
| 16 | Targeting HECTD3-IKKα axis inhibits inflammation-related metastasis显示文摘Metastasis is the leading cause of cancer-related death.The interactions between circulating tumor cells and endothelial adhesion molecules in distant organs is a key step during extravasation in hematogenous metastasis.Surgery is a common intervention for most primary solid tumors.However,surgical trauma-related systemic inflammation facilitates distant tumor metastasis by increasing the spread and adhesion of tumor cells to vascular endothelial cells(ECs).Currently,there are no effective interventions to prevent distant metastasis.Here,we show that HECTD3 deficiency in ECs significantly reduces tumor metastasis in multiple mouse models.HECTD3 depletion downregulates expression of adhesion molecules,such as VCAM-1,ICAM-1 and E-selectin,in mouse primary ECs and HUVECs stimulated by inflammatory factors and inhibits adhesion of tumor cells to ECs both in vitro and in vivo.We demonstrate that HECTD3 promotes stabilization,nuclear localization and kinase activity of IKKa by ubiquitinating IKKa with K27-and K63-linked polyubiquitin chains at K296,increasing phosphorylation of histone H3 to promote NF-kB target gene transcription.Knockout of HECTD3 in endothelium significantly inhibits tumor cells lung colonization,while conditional knockin promotes that.IKKa kinase inhibitors prevented LPS-induced pulmonary metastasis.These findings reveal the promotional role of the HECTD3-IKKa axis in tumor hematogenous metastasis and providea potential strategy for tumormetastasis prevention. | Fubing Li Huichun Liang Hua You Ji Xiao Houjun Xia Xi Chen Maobo Huang Zhuo Cheng Chuanyu Yang Wenjing Liu Hailin Zhang Li Zeng Yingying Wu Fei Ge Zhen Li Wenhui Zhou Yi Wen Zhongmei Zhou Rong Liu Dewei Jiang Ni Xie Bin Liang Zhenzhen Liu Yanjie Kong Ceshi Chen | 2022 | Signal Transduction and Targeted Therapy2022，7，9： | 1 |
| 17 | A Review on the Heat Pipe Photovoltaic/Thermal(PV/T)System显示文摘The Photovoltaic/thermal(PV/T)system combines the conventional PV panel with solar collector into one integrated system,which could achieve the function of generating power and providing thermal energy at the same time.Recently,it has become the most promising solar system for building applications.Most of the PV/T systems use water as the coolant,which could cause freezing problem in winter.To overcome this problem,the heat pipe PV/T system is developed to provide electrical and thermal energy stably without the seasonal barrier.Although some published review works have involved this type of PV/T system,they just stated a simple introduction on it,acting as a small part of their works.This paper focuses on the heat pipe PV/T system independently and provides a comprehensive and in-depth analysis of its performance.Firstly,the structure and operational principles of the heat pipe PV/T module and system are introduced concisely.Then the features and performance of different types of heat pipe PV/T systems,i.e.,integral heat pipe,loop heat pipe,and pulsating heat pipe PV/T system,are presented and analyzed.This is followed by the review on the performance of the systems which combine heat pipe PV/T module and other devices.Finally,the research gaps in this field are identified,and some future research trends and directions are recommended. | ZHOU Jinzhi ZHONG Wei WU Dan YUAN Yanping JI Wenhui HE Wei | 2021 | Journal of Thermal Science2021，30，5： | 1 |
| 18 | Mapping the distributions of blood-sucking mites and mite-borne agents in China:a modeling study显示文摘Background:Emerging mite-borne pathogens and associated disease burdens in recent decades are raising serious public health concerns,yet their distributions and ecology remain under-investigated.We aim to describe the geographical distributions of blood-sucking mites and mite-borne agents and to assess their ecological niches in China.Methods:We mapped 549 species of blood-sucking mites belonging to 100 genera at the county level and eight mite-associated agents detected from 36 species of blood-sucking mites in China during 1978–2020.Impacts of climatic and environmental factors on the ecology of 21 predominant vector mites and a leading pathogen,Orientia tsutsugamushi,were assessed using boosted regression tree(BRT)models,and model-predicted risks were mapped.We also estimated the model-predicted number,area and population size of afected counties for each of the 21 mite species in China.Results:Laelaps echidninus is the leading mite species that potentially afects 744 million people,followed by La.jettmari(517 million)and Eulaelaps stabularis(452 million).Leptotrombidium scutellare is the mite species harboring the highest variety of mite-borne agents including four Rickettsia species and two viruses,followed by Eu.stabularis(2 agents),L.palpale(2)and La.echidninus(2).The top two agents that parasitize the largest number of mite species are O.tsutsugamushi(28 species)and hantavirus(8).Mammalian richness,annual mean temperature and precipitation of the driest quarter jointly determine the ecology of the mites,forming four clusters of major mite species with distinct geographic distributions.High-risk areas of O.tsutsugamushi are mainly distributed in southern and eastern coastal provinces where 71.5 million people live.Conclusions:Ecological niches of major mite species and mite-borne pathogens are much more extensive than what have been observed,necessitating expansion of current fled surveillance. | Tao Wang Fanfei Meng Tianle Che Jinjin Chen Haiyang Zhang Yang Ji Zhengwei Fan Guoping Zhao Wenhui Zhang Baogui Jiang Qiang Xu Chenlong Lv Taoxing Shi Shiman Ruan Lanzheng Liu Wei Liu Yang Yang Liqun Fang | 2022 | Infectious Diseases of Poverty2022，11，2： | 0 |
| 19 | Targeted deep sequencing reveals the genetic heterogeneity in well-differentiated pancreatic neuroendocrine tumors with liver metastasis显示文摘Background:Pancreatic neuroendocrine tumor is a rare and heterogeneous entity,and approximately half of the patients harbored liver metastasis when initially diagnosed,whose prognosis is dismal.High-throughput sequencing has largely uncovered the genomic features of pancreatic neuroendocrine tumor,but the genetic alterations in the metastatic cases remain relatively unclear,which we aimed to study.Methods:Pathologically confirmed well-differentiated pancreatic neuroendocrine tumor samples resected in our hospital from 2000 to 2019 were collected.We performed deep sequencing on the exome of 341 tumor-related genes,and compared the differences of genetic alterations between the metastatic and the non-metastatic cases,as well as between the primary and the paired liver metastatic tumors.Results:Sequencing data of 79 samples from 29 pancreatic neuroendocrine tumor patients were included into analysis.A total of 2,471 somatic variants were identified,75.5%of which were considered as low-abundance.NOTCH1 was the most frequently mutated gene,altered in 26(53.1%)pancreatic neuroendocrine tumor samples from 18(62.1%)patients.Compared with the non-metastatic pancreatic neuroendocrine tumors,the metastatic cases were discovered with more single nucleotide variants and copy number variations,indicating the increased genomic instability.In addition,among the paired metastatic cases,the primary and the metastatic lesions shared limited mutated genes.Conclusions:Through the targeted deep sequencing,we identified the intratumor,intraindividual,and interindividual heterogeneity in the pancreatic neuroendocrine tumor patients,particularly in the metastatic cases,bringing potential challenges for the current biopsy strategies in guiding clinical treatments. | Wentao Zhou Xu Han Yuan Ji Dansong Wang Dong Xie Zilong Qiu Wenhui Lou | 2023 | Hepatobiliary Surgery and Nutrition2023，12，3： | 0 |
| 20 | Interactions of human islet amyloid polypeptide with lipid structure of different curvatures显示文摘Curvature is one of the most important features of lipid membranes in living cells,which significantly influences the structure of lipid membranes and their interaction with proteins.Taken the human islet amyloid polypeptide(h IAPP),an important protein related to the pathogenesis of type II diabetes,as an example,we performed molecular dynamics(MD)simulations to study the interaction between the protein and the lipid structures with varied curvatures.We found that the lipids in the high curvature membrane pack loosely with high mobility.The h IAPP initially forms H-bonds with the membrane surface that anchored the protein,and then inserts into the membrane through the hydrophobic interactions between the residues and the hydrophobic tails of the lipids.h IAPP can insert into the membrane more deeply with a larger curvature and with a stronger binding strength.Our result provided important insights into the mechanism of the membrane curvature-dependent property of proteins with molecular details. | Le Mei Wenhui Shen Xuwei Wu Jie Liu Dechang Li Baohua Ji | 2020 | Theoretical & Applied Mechanics Letters2020，10，6： | 0 |
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