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| 1 | Involvement of CsWRKY70 in salicylic acid-induced citrus fruit resistance against Penicillium digitatum显示文摘Penicillium digitatum causes serious losses in postharvest citrus fruit.Exogenous salicylic acid(SA)can induce fruit resistance against various pathogens,but the mechanism remains unclear.Herein,a transcriptome-based approach was used to investigate the underlying mechanism of SA-induced citrus fruit resistance against P.digitatum.We found that CsWRKY70 and genes related to methyl salicylate(MeSA)biosynthesis(salicylate carboxymethyltransferase,SAMT)were induced by exogenous SA.Moreover,significant MeSA accumulation was detected in the SA-treated citrus fruit.The potential involvement of CsWRKY70 in regulating CsSAMT expression in citrus fruit was studied.Subcellular localization,dual luciferase,and electrophoretic mobility shift assays and an analysis of transient expression in fruit peel revealed that the nucleus‐localized transcriptional activator CsWRKY70 can activate the CsSAMT promoter by recognizing the W-box element.Taken together,the findings from this study offer new insights into the transcriptional regulatory mechanism of exogenous SA-induced disease resistance in Citrus sinensis fruit. | Bing Deng Wenjun Wang Changqing Ruan Lili Deng Shixiang Yao Kaifang Zeng | 2020 | Horticulture Research2020，7，1： | 6 |
| 2 | Differential DNA methylation profiles of human B lymphocytes and Epstein-Barr virus-immortalized B lymphocytes显示文摘Objective: This study aimed to comprehensively assess Epstein-Barr virus(EBV)-induced methylation alterations of B cell across whole genome.Methods: We compared DNA methylation patterns of primary B cells and corresponding lymphoblastoid cell lines(LCLs) from eight participants. The genome-wide DNA methylation profiles were compared at over 850,000 genome-wide methylation sites.Results: DNA methylation analysis revealed 87,732 differentially methylated Cp G sites, representing approximately 12.41% of all sites in LCLs compared to primary B cells. The hypermethylated and hypomethylated Cp G sites were about 22.75% or 77.25%, respectively. Only 0.8% of hypomethylated sites and 4.5% of hypermethylated sites were located in Cp G islands, whereas 8.0% of hypomethylated sites and 16.3% of hypermethylated sites were located in shore(N_shore and S_shore). Using principal component analysis of the DNA methylation profiles, primary B cells and LCLs could be accurately predicted. Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis of differently methylated genes revealed that most of the top GO biological processes were related to cell activation and immune response, and some top enrichment pathways were related with activation and malignant transformation of human B cells.Conclusions: Our study demonstrated genome-wide DNA methylation variations between primary B cells and corresponding LCLs, which might yield new insight on the methylation mechanism of EBV-induced immortalization. | Chaoting Zhang Rui Zhang Zhiqiang Chen Jing Chen Jigang Ruan Zheming Lu Hongchao Xiong Wenjun Yang | 2018 | Chinese Journal of Cancer Research2018，30，1： | 1 |
| 3 | Three- dimensional effective elastic moduli for multidirectional filament- wound tube with lining 显示文摘 | Xu Guanglei Ruan Wenjun Wang Hao | 2011 | Advanced Materials Research2011，，： | 1 |
| 4 | Effect of nickel ion from autoclave material on oxidation behaviour of 304 stainless steel in oxygenated high temperature water显示文摘 | Wenjun Kuang Xinqiang Wu En-Hou Han Liqun Ruan | 2010 | Corrosion Science2010，，3： | 1 |
| 5 | Research on Designing Profiled Rod Warhead显示文摘A new Kinetic Energy Rod( KER) warhead named profiled rod warhead is proposed in this paper.Based on the design of profiled rod warhead,a model of profiled rod driven by detonation is established. The detonation process is simulated by ANSYS / LS-DYNA,and the deployment velocity and initial flight attitude of rod are achieved. In addition,static rod deployment testing are performed to investigate the damage effect,the spatial flight attitude and deployment velocity. A satisfactory agreement is obtained by the comparison between numerical results and testing results. Meanwhile,the profiled rod studies are conducted to determine a higher penetrability compared with traditional cylindrical rods. Rigid body dynamics equations of profiled rod,which accounts for the influence of air resistance,are set up to predict the flight trajectory of long-distance. The results show that the profiled rod may provide a better penetration angle which still maintains a significant penetrability against projectiles when the rods move off long-distance range. | Huijun Ning Hao Wang Cheng Zhang Dongyang Chen Wenjun Ruan | 2015 | Journal of Harbin Institute of Technology(New Series)2015，22，4： | 0 |
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