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| 1 | Antibacterial activity of leaves and inter-nodal callus extracts of Mentha arvensis L显示文摘Objective:To determine the anti-bacterial efficacy of chloroform,ethanol,ethyl acetate and water extracts of inter-nodal and leaves derived calli extracts from Mentha arvensis(M.arvensis) against Salmonella typhi(S.typhi),Streptococcus pyogenes(S.pyogenes),Proteus vulgaris(P. vulgaris) and Bacillus subtilis(B.subtilis).Methods:The inter-nodal and leaves segments of M.arvensis were cut into 0.5-0.7 cm in length and cultured on Murashige and Skoog solid medium supplemented with 3%sucrose,gelled with 0.7%agar and different concentration of 2,4-Dichlorophenoxyacetie acid(2,4-D) either alone or in combinations.The preliminary phytochemical screening was performed by Brindha et al method.Antibacterial efficacy was performed by disc diffusion method and incubated for 24 h at 37℃.Results:Maximum percentage of callus formation(inter-nodal segments 84.3±0.78;leaves segments 93.8±1.27) was obtained on Murashige and Skoog’s basal medium supplemented with 3%sucrose and 1.5 mg/L of 2,4-D.The ethanol extracts of leaves derived calli showed the maximum bio-efficacy than other solvents.The leaves and stem derived calli extracts on Proteus sp.showed that the plants can be used in the treatment of urinary tract infection associated with Proteus sp.Through the bacterial efficacy studies,it is confirmed that the in vitro raised calli tissue was more effective compared to in vivo tissue.Conclusions:The bio-efficacy study confirmed that the calli mediated tissues showed the maximum zone of inhibition.The present study paved a protocol to establish high potential cell lines by in vitro culture. | Johnson M Wesely EG Kavitha MS Uma V | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，3： | 9 |
| 2 | Chromatographic finger print analysis of steroids in Aerva lanata L by HPTLC technique显示文摘Objective:To determine the chemical profile and steroids composition of the medicinally important plant Aerva lanata(A.lanata) L.Methods:Preliminary phytochemicul screening was done by the method as Harborne described.HPTLC studies were canied out as Harborne and Wagner et al described.The Ethyl acetate-ethanol-water(8:2:1.2) was employed as mobile phase for glycosides.Results:The desired aim was achieved using Chloroform-acetone(8:2) as the mobile phase.The methanolic extract of stem,leaves,root,flower and seeds of A.lanata showed the presence of 30 different types of steroids with 30 different Rf values from 0.04 to 0.97. Maximum number(11) of steroids has been observed in leaves followed by root(10).Conclusions: HPTLC profile of steroids has been chosen here to reveal the diversity existing in A.lanata.Such finger printing is useful in differentiating the species from the adulterant and act as biochemical markers for this medicinally important plant in the pharma industry and plant systematic studies. | Yamunadevi Mariswamy Wesely Edward Gnaraj Johnson M | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，6： | 6 |
| 3 | In vivo and in vitro phytochemical and antibacterial efficacy of Baliospermum montanum (Willd.) Muell.Arg.显示文摘Objective:To evaluate the phytochemical and anti-bacterial potential of mother plants in vivo and in vitro derived callus of Baliospermum montanum(B.montanum)(Willd.) Muell.-Arg. leaves and root.Methods:The in vitro derived rootlets and leaves segments of B.montanum were cut into 0.5-0.7 cm in length and cultured on Murashige and Skoog solid medium supplemented with 3%sucrose,gelled with 0.7%agar and different concentration of 2,4-D either alone or in combinations.The preliminary phytochemical screening was performed by Harborne method.Antibacterial efficacy was performed by well diffusion method and incubated for 24 h at 37℃.Results:The highest percentage of callus formation(leaves segments 86.9±0.56:root segments 78.7±0.51) was obtained on Murashige and Skoog’s basal medium supplemented with 3%sucrose and 2.0 mg/L of 2.4-Dichlorophenoxy acetic acid.The phytochemical study revealed the high quantity presence of steroids,triterpenoids,glycosides,saponins,alkaloids,flavanoids, phenolic compounds,tannins,sugars etc of root and leaves derived calli.The ethanol extract of leaves segment derived calli of B.montanum showed the maximum solubility and antimicrobial activity with the MIC ranged from 100 to 200μL.Conclusions:The preliminary phytochemical study confirmed that the calli mediated tissues showed the higher percentage of metabolite constituents and extraction value compared to the in vivo leaves and roots.The present study observation suggested that a possibility to establish high yielding genotypes by in vitro culture for production of medicinally important bioactive compounds. | Johnson M Wesely EG Zahir Hussain MI Selvan N | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，11： | 6 |
| 4 | The effect of leaves extracts of Clitoria ternatea Linn against the fish pathogens显示文摘Objective:To investigate the antimicrobial activity of Clitoria ternatea(C.ternatea) against the fish pathogens viz.,Pseudomonas aeruginosa(P.aeruginosa),Escherichia coli(E.coli),Klebsiella pneumonia(K.pneumonia),Bacillus subtilis(B.subtilis),Aeromonas formican(A.formicans)s, Aeromonas hydrophila(A.hydrophila) and Streptococcus agalactiae(S.agalactiae )isolated from diseased Tilapia(Oreochromis niloticus).Methods:The extracts of C.ternatea was tested against P.aeruginosa,E.coli,K.pneumonia,B.subtilis,A.formicans,A.hydrophila and S.agalactiae by the agar well diffusion method.Results:Different extracts of C.ternatea showed inhibitory effects against P.aeruginosa,E.coli,K.pneumonia,B.subtilis,A.formicans,A.hydrophila and S. agalactiae.Ethyl acetate extracts of C.ternatea showed maximum of zone of inhibition against A. formicans(18 mm),A.hydrophilia(19 mm),B.subtilis(19 mm) and P.aeruginosa(21 mm) next to that ethanol extract of C.ternatea showed A.formicans(18 mm) and E.coli(14 mm) followed by Acetone extract showed maximum zone of inhibition S.agalactiae(19 mm) and K.pneumonia(17 mm).Conclusions:The antimicrobial activities of all the four plant extracts are comparable and their potential as alternative in the treatment of infectious by these microorganisms was present in the fish.Susceptibility testing is conducted on isolates using drugs selected on the basis of their importance to human medicine and use in fish production. | Selvamaleeswaran Ponnusamy Wesely Ebenezer Gnanaraj Johnson Marimuthu Antonisamy Velusamy Selvakumar Jeyakumar Nelson | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，9： | 4 |
| 5 | A review of the current status of knowledge on dry deposition显示文摘 | Wesely ML Hicks BB | | 0，，： | 1 |
| 6 | Parameterization of surface resistance to gaseous dry deposition in regional-scale numerical models显示文摘 | Wesely M L | 1989 | Atmospheric Environment1989，23，6： | 1 |
| 7 | Parameterization of surface resistances to gaseous dry deposition in regional-scale numerical models 显示文摘 | WESELY M L | 1989 | Atmospheric Environment1989，23，： | 1 |
| 8 | Collagenase induced intracerebral hemorrhage in rats 显示文摘 | Mun-Bryce S Wesely M | 1990 | Stroke1990，21，： | 1 |
| 9 | An algorithm for planning colli- sion-free paths among polyhedral obstacles 显示文摘 | Lozano-Perez T Wesely M | 1979 | Commun of the ACM1979，22，10： | 1 |
| 10 | Collagenase-induced intracerebral hemorrhage in rats显示文摘 | Rosenberg GA Mun-Bryce S Wesely M | 1990 | Stroke1990，21，3： | 1 |
| 11 | Parameterization of surface resistances to gaseous dry deposition in regional-scale numerical models显示文摘 | M.L. Wesely | 2007 | Atmospheric Environment2007，，： | 1 |
| 12 | 'Park'Kentucky bluegrass response to fpliarly applied urea显示文摘 | Wesely R W Shearman R C KinbacherE J | 1988 | Horticultural Science1988，23，： | 1 |
| 13 | Foliar N-uptake by eight turfgrass grown in controlled environment显示文摘 | Wesely R W Shearman R C Kinbacher E J | 1985 | Journal of the American Society for Horticultural Science1985，110，5： | 1 |
| 14 | Numerical modeling of the turbulent fluxes of chemically reactive trace gases in the atmospheric boundary layer 显示文摘 | Gao BY Wesely M | 1994 | Journal of Applied Meteorology1994，33，7： | 1 |
| 15 | Modeling gaseous dry deposition over regional scales with satellite observations-L Model development 显示文摘 | Gao W Wesely M | 1995 | Atmospheric Environment1995，29，6： | 1 |
| 16 | Parameterization of surface resistance to gaseous dry deposition in regional scale models 显示文摘 | WESELY M L | 1989 | Atmospheric Environment1989，23，6： | 1 |
| 17 | Modification of coded parameterizations of surface resistances to gaseous dry deposition显示文摘 | Walmsley J L Wesely M L | 1996 | Atmospheric Environment1996，30，7： | 1 |
| 18 | Estimates of biogenic emissions using satellite observations and influence of isoprene emission on O 3 formation over the eastern United States显示文摘 | Yiwen Xu M.L. Wesely T.E. Pierce | 2002 | Atmospheric Environment2002，，38： | 1 |
| 19 | Parameterization of surface resistance to gaseous dry deposition in regional-scale numerical models显示文摘 | Wesely M L | 1989 | Atmospheric Environment1989，23，6： | 1 |
| 20 | Parameterizations of surface resistances to gaseous dry deposition in regional-scale numerical models显示文摘 | WESELY M L | 1989 | Atmos Environ1989，23，： | 1 |
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