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| 1 | Antimicrobial activity,cytotoxicity,and phytochemical screening of Voacanga globosa(Blanco) Merr.leaf extract(Apocynaceae)显示文摘Objective:To determine the antibacterial,antifungal,antiprotozoal,cytotoxic,and phytochemical properties of ethanol extracts of leaves of Voacanga globosa(Blanco) Merr.(V.globosa). Methods:The extracts were tested against bacteria and fungus through disc diffusion assay; against protozoa through growth curve determination,antiprotozoal and cytotoxicity assays. Results:The extract revealed antibacterial activities,inhibiting the growth of Staphylococcus aureus,Bacillus cereus,Pseudomonas aeruginosa,Micrococcus luteus,and Salmonella typhimurium.Antifungal assay showed that it inhibited Candida albicans.The antiprotozoal assay against Trichomonas vaginalis and Entamoeba histolytica showed that V.globosa can inhibit the parasites,wherein the action can be comparable to metronidazole.With the in situ cell death detection kit.Trichomonas vaginalis and Entamoeba histolytica exposed to V.globosa leaf extract was observed to fluoresce simultaneously in red and yellow signals signifying apoptotic-like changes.Preliminary phytochemical screening revealed the chemical composition of plant extract containing alkaloids,saponins,2-deoxysugars,and hydrolysabie tannins.Conclusions: Thus,thus study provides scientific evidence on the traditional use of V.globosa leaf extract in treating microbial diseases.Further,the leaf extract can possibly be used to produce alternative forms of antimicrobials. | Pierangeli G Vital Windell L Rivera | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，10： | 5 |
| 2 | Comparison of direct fecal smear microscopy,culture,and polymerase chain reaction for the detection of Blastocystis sp.in human stool samples显示文摘Objective:To compare the sensitivity and specificity of direct fecal smear microscopy,culture,and polymerase chain reaction in the detection of Blastocystis sp.in human stool.Methods:Human stool samples were collected from a community in San Isidro,Rodriguez,Rizal,Philippines.These samples were subjected to direct fecal smear microscopy,culture and polymerase chain reaction to detect the presence of Blastocystis sp.Results:Of the 110 stool samples collected,28(25%)were detected positive for the presence of Blastocystis sp.by two or more tests.Culture method detected the highest number of Blastocystis-positive stool samples(n=36),followed by PCR of DNA extracted from culture(n=26),PCR of DNA extracted from stool(n=10),and direct fecal smear(n=9).Compared to culture,the sensitivity of the other detection methods were 66.7%for PCR from culture and 19.4%for both PCR from stool and direct fecal smear.Specificity of the methods was high,with PCR from culture and direct fecal smear having97.3%,while PCR from stool at 95.9%.Conclusions:In this study,in vitro culture is the best method for detecting Blastocystis sp.in human stool samples. | Herbert J Santos Windell L Rivera | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，10： | 3 |
| 3 | Science显示文摘 | JONES D J DIDDAMS S A RANKA J K STENT J A WINDELE R S HALL J L CUNDIFF S T | 2000 | ( 288 ): 6352000，，288： | 1 |
| 4 | Blood glucose and beta - hydroxybutyrate responses when the insulin pump is stopped in children and adolescents显示文摘 | Hanas R Lundqvist K Windell L el al | 2006 | Pediatr Diabetes2006，7，5： | 1 |
| 5 | A review of the effects of molds and mycotoxins in mminants显示文摘 | Dicostanzo A Johnston L Windels H | 1995 | Professional Animal Scientist1995，12，： | 1 |
| 6 | 查看详情显示文摘 | Dudley J M Gu X Xu L Coen S Windeler R S | | 0，，： | 1 |
| 7 | Femtosecond laser based synthesis of ultrastable microwave signals from optical frequency references显示文摘 | Diddams S A Oates C W Wilpers G Windeler R S Bergquist J C Oskay W H Hollberg L | 2004 | Opt Lett2004，30，： | 1 |
| 8 | 查看详情显示文摘 | Diddams S A Jones D J Ye J Cundiff S T Hall J L Ranka J K Windeler R S Holzwarth R Udem T Auml Nsch T W | | 0，，： | 1 |
| 9 | 显示文摘 | Popplewell J Charles S W | 1975 | IEEE Trans Magn1975，11，： | 1 |
| 10 | Randomized trial of rate controlversus rhythm control in persistent atrial fibrillation: The Strategies of Treatment of Atrial Fibrillation(STAF)trial显示文摘 | Carlsson L Miketie S Windeler J | 2003 | J Am CollCardiol2003，41，： | 1 |
| 11 | Profiles of Entamoeba histolytica—specific imunoglobulins in human sera显示文摘Objective:To determine the profdes of anti-Entamoeba histolytica(E.histolytica) IgA.IgC. and IgM in sera of diarrheic and non-diarrheic individuals and partially characterize target antigens.Methods:Serum samples from thirty diarrheic and thirty non-diarrheic individuals were subjected to IgA,IgG,and IgM profiling through enzyme-linked immunosorbent assay (EI.ISA),flow cytometry,and immunoblot.Results:ELBA titer results showed that both diarrheic and non-diarrheic individuals possess high levels of E.histolytica-specific IgG compared to IgA and IgM.How cytometry data showed that diarrheic serum samples had higher mean reaction percentages against E.histolytica cells compared to non-diarrheic samples.Immunoreactive E.histolytica proteins with molecular weights ranging belween 7 kDa and 292 kDa were recognized by diarrheic serum IgG,and 170 kDa and 250 kDa by non-diarrheic serum IgG. Conclusions:Our findings suggest that serum anti-E.histolytica IgG,compared with serum anti-E.histolytica IgA and IgM responses,was generally high in both diarrheic and nondiarrheic sera,indicating a past exposure to the organism both in symptomatic patients as well as in asymptomatic carriers,respectively.In addition,serum IgG from diarrheic and non-diarrheic patients were able to detect immunogenic E.histolytica proteins. | Windell L Rivera Herbert J Santos Vanissa A Ong Lara Jessica G Murao | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，3： | 0 |
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