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| 1 | Induction of apoptosis by arsenic trioxide and hydroxycamptothecin in gastric cancer cells in vitro显示文摘AIM To study the effects of arsenic trioxide andHCPT on different degrees of differentiated gastriccancer cells(SGC-7901,MKN-45,MKN-28)withrespect to both cytotoxicity and induction ofapoptosis in vitro.METHODS The cytotoxicity of As2O3 and HCPTon gastric cancer cells was determined by MTTassay.Morphologic changes of apoptosis ofgastric cancer cells were observed by lightmicroscopy and transmission electron microscopy.Apoptosis and cell cycle changes of gastric cancercells induced by HCPT and As2O3 were investigatedby TUNEL method and flow cytometry.RESULTS As2O3 and HCPT had remarkablecytotoxic effects on different degrees ofdifferentiated gastric cancer cells.The IC50ofAs2O3 on well differentiated gastric cancer cellMKN-28,moderately differentiated gastric cancercell SGC-7901,and poorly differentiated gastriccancer cell MKN-28 were 8.91 μmol/L,10.57μmol/L,and 11.65 μmol/L,respectively.The IC50of HCPT on MKN-28,SGC-7901,and MKN-45 were9.35 mg/L,10.21 mg/L,and 12.63 mg/Lrespectively after 48 h treatment.After 12 h ofexposure to both drugs,gastric cancer cellsexhibited morphologic features of apoptosis,including cell shrinkage,nuclear condensation, and formation of apoptotic bodies.A typicalsubdiploid peak before G0/G1 phase was observedby flow cytometry.The apoptotic rates of SGC-7901,MKN-45,and MKN-28 were 13.84%,22.52%,and 9.68%,respectively after 48 hexposure to 10 μmol/L As2O3.The apoptotic ratesof SGC-7901,MKN-45,and MKN-28 were 21.88%,12.35%,and 30.26%,respectively after 48 hexposure to 10 mg/L HCPT.The apoptotic indicewere 7%-15% as assessed by TUNEL method.The effect of As2O3 on SGC-7901 showedremarkable cell cycle specificity,which inducedcell death in G1 phase,and blocked G2/M phase.HCPT also showed a remarkable cell cyclespecificity,by inducing cell death and apoptosis inG1 phase and arrest of proliferation at S phase.CONCLUSION As2O3 and HCPT exhibitsignificant cytotoxicity on gastric cancer cells byinduction of apoptosis.As2O3 and HCPT mighthave a promising prospect in the treatment ofgastric cancer,which needs to be further studied. | Tu SP Zhong J Tan JH Jiang XH Qiao MM Wu YX Jiang SH | 2000 | World Journal of Gastroenterology2000，6，4： | 43 |
| 2 | Isolation and Structure Elucidation of Autolytimycin, A New Compound Produced by Streptomyces Autolyticus JX-47显示文摘Autolytimycin 1 was isolated from the culture filtrate of Streptomyces autolyticus JX-47, together with two known compounds, lebstatin 2 and 17-O-demethyl-geldanamycin 3. These compounds showed the activities of anti-HSV-I. The structure of 1 was determined by spectral analysis. | Li, MG Wu, SH Zhao, LX Zhang, Q Li, WJ Cui, XL Xu, LH Wu, DG Jiang, CL | 2001 | Chinese Chemical Letters2001，12，10： | 10 |
| 3 | Overexpression/amplification of HER-2/neu is uncommon in hepatocellular carcinoma显示文摘 | Xian ZH Zhang SH Cong WM Wu WQ Wu MC | 2005 | 第二军医大学学报2005，26，11： | 6 |
| 4 | Expression of aspartyl beta-hydroxylase and its clinicopathological significance in hepatocellular carcinoma显示文摘 | Xian ZH Zhang SH Cong WM Yan HX Wang K Wu MC | 2006 | 第二军医大学学报2006，27，5： | 6 |
| 5 | A New Ursene Type Triterpenoid from Crepis napifera显示文摘A new triterpenoid, urs-20-en-3β-acetoxy-22α-ol, and three known compounds 3β- acetoxy-amyrin, 3β-acetoxy-lupeol, lupeol were isolated from the leaves of Crepis napifera (Franch.) Babc. Their structures were determined by means of spectroscopic studies. | Wu, SH Luo, XD Ma, YB Wu, DG | 2000 | Chinese Chemical Letters2000，11，8： | 3 |
| 6 | Lead-promoted Barbier-type reaction of aldehydes with propargyl bromides in aqueous media显示文摘This paper reports that the reaction of the propargyl bromides with aldehydes promoted by powdered lead in aqueous media.The selectivity and possible mechanism of these reactions are discussed.The yields of products for reaction of propargyl bromide are 31~71%.The ratios of al-lenyl alcohol and homopropargyl alcohol are 1:1.5 to 1:3.The product for reaction of phenyl propargyl bromide is allenyl alcohol and the yields are 52~84%. | Zhou, JY Sun, GF Zhang, MF Jia, Y Wu, SH | 1997 | Chinese Journal of Chemistry1997，15，4： | 2 |
| 7 | Recent advances in understanding aminoglycoside ototoxicity and its prevention显示文摘 | Wu WJ Sha SH Schacht J | 2002 | Audiol Neurootol2002，7，： | 1 |
| 8 | Tribendimidine: a promis- ing, safe and broad-spectrum anthelmintic agent from China 显示文摘 | Xiao SH Wu HM Tanner M | 2005 | Acta Trop2005，94，1： | 1 |
| 9 | Intratympanic steroid injections as a salvage treatment for sudden sensorineural hearing loss: a randomized, double-blind, placebo-controlled study 显示文摘 | Wu HP Chou YF Yu SH | 2011 | Otol Neurotol2011，32，5： | 1 |
| 10 | Diagnostic value of procalcitonin forbacterial infection in elderly patients-a systemic review and meta-a-nalysis显示文摘 | Lee SH Chan RC Wu JY | 2013 | Int J Clin Pract2013，67，12： | 1 |
| 11 | Membrane resonance and subthreshold membrane oscillations in mesencephalic V neurons:participants in burst generation 显示文摘 | Wu N Hsiao CF Chandler SH | 2001 | J Neurosci2001，21，： | 1 |
| 12 | Inverse temporal changes of lipoxin A4 and leuko/rienes in children with Henoch-SchOnlein purpura 显示文摘 | Wu SH Liao PY Yin PL | 2009 | Prostaglandins Leukot Essent Fatty Acids2009，80，4： | 1 |
| 13 | Specific immunotherapy suppresses Th2 responses via modulating TIM1/ TIM4 interaction on dendritic cells显示文摘 | Zhao CQ Li TL He SH Chen X An YF Wu WK | 2010 | Allergy2010，65，8： | 1 |
| 14 | Factors associated with the stability of mini - implants for orthodontic anchorage:a study of 414 samples in Taiwan 显示文摘 | Wu TY Kuang SH Wu CH | 2009 | J Oral Maxillofac Surg2009，67，8： | 1 |
| 15 | Prevalence of Plasmid-Mediated Quinolone Resistance Determinants, QnrA, QnrB, and QnrS, among Clinical Isolates of Enterobacter cloacae in a Taiwanese Hospital 显示文摘 | Wu J J Ko WC Tsai SH | 2007 | Antimicrob Agents Chemother2007，51，4： | 1 |
| 16 | Evaluation of the nursing intervention classification for use by llight nurses显示文摘 | Wu SH Thompson CB | 2001 | Air Med J2001，20，1： | 1 |
| 17 | Reappraisal of the relation between blood lead concentration and blood pressure among the general population in Taiwan显示文摘 | Liou SH Wu TN | 1999 | Occup Environ Med1999，56，1： | 1 |
| 18 | Inactivation of erythropoietin Leads to defects in cardiac morphogenesis显示文摘 | Wu H Lee SH Gao J | 1999 | Development1999，126，16： | 1 |
| 19 | Prevalence of plasmid-mediated quinolone resistance determinants QnrA, QnrB, and QnrS among clinical isolates of Enterobacter cloacae in a Taiwanese hospital显示文摘 | Wu JJ Ko WC Tsai SH | 2007 | Antimierob Agents Chemother2007，51，4： | 1 |
| 20 | UPR pathways combine to pre- vent hepatic steatosis caused by ER stress - mediated suppression of transcriptional master regulators 显示文摘 | Rutkowski DT Wu J Back SH | 2008 | Dev Cell2008，15，6： | 1 |
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