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| 1 | Suppression of P-gp induced multiple drug resistance in a drug resistant gastric cancer cell line by overexpression of Fas显示文摘AIM To observe the drug sensitizing effect andrelated mechanisms of fas gene transduction onhuman drug-resistant gastric cancer cellSGC7901/VCR(resistant to Vincristine).METHODS The cell cycle alteration wasobserved by FACS.The sensitivity of gastriccancer cells to apoptosis was determined by invitro apoptosis assay.The drug sensitization ofcells to several anti-tumor drugs was observedby MTT assay.Immunochemical method wasused to show expression of P-gp and Topo Ⅱ ingastric cancer cells.RESULTS Comparing to SGC7901 and pBK-SGC7901/VCR,fas-SGC7901/VCR showeddecreasing G2 cells and increasing S cells,theG2 phase fraction of pBK-SGC7901/VCR wasabout 3.0 times that of fas-SGC7901/VCR,but Sphase fraction of fas-SGC7901/VCR was about1.9 times that of pBK-SGC7901/VCR,indicatingS phase arrest of fas-SGC7901/VCR.FACS alsosuggested apoptosis of fas-SGC7901/VCR,fas-SGC7901/VCR was more sensitive to apoptosisinducing agent VM-26 than pBK-SGC7901/VCR.MTT assay showed increased sensitization offas-SGC7901/VCR to DDP,MMC and 5-FU,butsame sensitization to VCR according to pBK-SGC7901/VCR.SGC7901,pBK-SGC7901/ VCRand fas-SGC7901/VCR had positively stainedTopo Ⅱ equally.P-gp staining in pBK- SGC7901/VCR was stronger than in SG07901,but there was little staining of P-gp in fas.SGC7901/VCR.CONCLUSION fas gene transduction couldreverse the MDR of human drug-resistant gastriccancer cell SGC7901/VCR to a degree,possiblybecause of higher sensitization to apoptosis anddecreased expression of P-gp. | Yin F Shi YQ Zhao WP Xiao B Miao JY Fan DM | 2000 | World Journal of Gastroenterology2000，6，5： | 24 |
| 2 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 3 | Cloning and identification of an angiostatic molecule IP-10/crg-2显示文摘AIM To obtain human and murine cDNAsencoding IFN--y inducible protein 10 (lP--10) andcytokine responsive gene--2 (Crg-2).METHODS The encoding genes of lP-10 and Crg2 were amplified by RT--PCR from Cultured humanfibroblast cells and Balb/ c mouse liver treatedby IFN-y and TNF-a, respectively, and clonedinto plasmids of PUC19 and PGEM3Zf(+ ).RESULTS The nucleotide sequences ot theamplified DNA were confirmed by endonucleasesdigestion and sequencing.CONCLUSION Recombinant lP-10/ crg--2 geneclones with 306 hp and 314 hp inserts wereestablished for further research on biologicalactivities and ligands of hiP-10/mCrg--2. | Liu ZG Yang JH An HZ Wang HY He FT Han ZY Han Y Wu HP Xiao B Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 2 |
| 4 | Bax Reversal of multidrug resistance of gastriccancercellline27901/VCRbyBaxgenetransduction显示文摘 | Zhao QY Xiao B Fan DM | 2000 | ChinJDigest2000，20，1： | 1 |
| 5 | Mutation in ribosomal proteins and ribosomal RNA confer macrolide resistance in human Ure- aplasmaspp显示文摘 | Xiao L Crabb DM Duffy LB | 2011 | Antimicrob Agents2011，37，4： | 1 |
| 6 | Involvement of multitargets in paeoniflorin-induced preconditioning 显示文摘 | Chen DM Xiao L Cai X | 2006 | J Pharmacol Exp Ther2006，319，1： | 1 |
| 7 | Resveratrol inhibits interleukin 1 β-mediated inducible nitric oxide synthase expression in articular chondrocytes by activating SIRT1 and thereby suppressing nuclear factor-κB activity显示文摘 | Lei M Wang JG Xiao DM | 2012 | Eur J Pharmacol2012，674，23： | 1 |
| 8 | Skin rash could predict the response to EGFR tyrosine kinase inhibitor and the prognosis for patients with non-small cell lung cancer:a systematic review and meta-analysis显示文摘 | Liu HB Wu Y Lv TF Yao YW Xiao YY Yuan DM | 2013 | PLo S One2013，8，55： | 1 |
| 9 | Possible effects of early treatments of hsp90 inhibitors on preventing the evolution of drug resistance to other anti-cancer drugs 显示文摘 | Xiao L Rasouli P Ruden DM | 2007 | Curr Med Chem2007，14，2： | 1 |
| 10 | Involvement of multitargets in paeoniflorin- induced preconditioning 显示文摘 | Chen DM Xiao L Cai X | 2006 | J Pharmacol Exp Ther2006，319，1： | 1 |
| 11 | Mutations in ribosomal proteins and ribosomal RNA confer macrolide resistance in human Ureaplasma spp显示文摘 | Xiao L Crabb DM Duffy LB | 2011 | Int J Antimicrob Agents2011，37，4： | 1 |
| 12 | TPX2 expression and its sig- nificance in squamous cell carcinoma of lung 显示文摘 | Lin DM Ma Y Xiao T | 2006 | Zhonghua Bing Li Xue Za Zhi2006，35，9： | 1 |
| 13 | SIMS zircon U-Pb geochronology and Sr-Nd isotopes of Ni-Cu-bearing mafic-ultramafic intrusions in Eastern Tianshan and Beishan in correlation with flood basalts in Tarim basin (NW China):constraints on a ca,280 Ma mande plume显示文摘 | Qin KZ Su BX Patrick Asamoah Sakyi Tang DM Li XH Xiao QH Liu PP | | 0，，： | 1 |
| 14 | Low-concentration heparin suppresses ionomycin-activated CAMK-Ⅱ/EGF receptor-and ERK- mediated signalingin mesangial cells显示文摘 | Song L Xiao W Templeton DM | 2010 | J Cell Physiol2010，224，2： | 1 |
| 15 | The change of CD4^+ CD25^high CCR6^+ regulatory T cells in breast cancer patients 显示文摘 | Xu L Zhou Y Xiao DM | 2010 | Sichuan Da Xue Xue Bao (Yi Xue Ban)2010，41，3： | 1 |
| 16 | Resveratrol inhibits interleukin l l3-mediated inducible nitric oxide synthase expression in articular chondrocytes by activating SIRT1 and thereby suppressing nuclear factor- KB activity 显示文摘 | Lei M Wang JG Xiao DM | 2012 | Eur J Pharmaeol2012，674，23： | 1 |
| 17 | Involvement of multitargets in paeoniflorin- induced preconditioning显示文摘 | Chen DM Xiao L Cai X | 2006 | J Pharmacol Exp Ther2006，106，10： | 1 |
| 18 | TPX2 expression and its significance in squamous cell carcinoma of lung显示文摘 | Lin DM Ma Y Xiao T | 2006 | Zhonghua Bing Li Xue Za Zhi2006，35，9： | 1 |
| 19 | Spontaneous activation of β2- but not β1-adrenoceptors expressed in cardiac myocytes from β1,β2 Double Knockout Mice显示文摘 | Zhou YY Yang DM Xiao RP | 2000 | Molecular Pharmacology2000，58，： | 1 |
| 20 | Chemical compositions andantimicrobial property of three edible and medicinal Cordycepsspecies显示文摘 | Xiao JH Xiao DM Sun ZH | 2009 | J Food Agriculture & Environment2009，7，34： | 1 |
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