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| 1 | Suppression of P-gp induced multiple drug resistance in a drug resistant gastric cancer cell line by overexpression of Fas显示文摘AIM To observe the drug sensitizing effect andrelated mechanisms of fas gene transduction onhuman drug-resistant gastric cancer cellSGC7901/VCR(resistant to Vincristine).METHODS The cell cycle alteration wasobserved by FACS.The sensitivity of gastriccancer cells to apoptosis was determined by invitro apoptosis assay.The drug sensitization ofcells to several anti-tumor drugs was observedby MTT assay.Immunochemical method wasused to show expression of P-gp and Topo Ⅱ ingastric cancer cells.RESULTS Comparing to SGC7901 and pBK-SGC7901/VCR,fas-SGC7901/VCR showeddecreasing G2 cells and increasing S cells,theG2 phase fraction of pBK-SGC7901/VCR wasabout 3.0 times that of fas-SGC7901/VCR,but Sphase fraction of fas-SGC7901/VCR was about1.9 times that of pBK-SGC7901/VCR,indicatingS phase arrest of fas-SGC7901/VCR.FACS alsosuggested apoptosis of fas-SGC7901/VCR,fas-SGC7901/VCR was more sensitive to apoptosisinducing agent VM-26 than pBK-SGC7901/VCR.MTT assay showed increased sensitization offas-SGC7901/VCR to DDP,MMC and 5-FU,butsame sensitization to VCR according to pBK-SGC7901/VCR.SGC7901,pBK-SGC7901/ VCRand fas-SGC7901/VCR had positively stainedTopo Ⅱ equally.P-gp staining in pBK- SGC7901/VCR was stronger than in SG07901,but there was little staining of P-gp in fas.SGC7901/VCR.CONCLUSION fas gene transduction couldreverse the MDR of human drug-resistant gastriccancer cell SGC7901/VCR to a degree,possiblybecause of higher sensitization to apoptosis anddecreased expression of P-gp. | Yin F Shi YQ Zhao WP Xiao B Miao JY Fan DM | 2000 | World Journal of Gastroenterology2000，6，5： | 24 |
| 2 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 3 | Collecting aerosol in airflow with a magnetically stabilized fluidized bed显示文摘A magnetically stabilized fluidized bed (MSB) is a highly efficient filter that takes the advantage of both fluidized beds and fixed beds. This paper presents the research to collect aerosol in airflow with a MSB. The filtering model of MSB is established with its parameters including magnetic field intensity, gas superficial velocity, average grain size, and bed height on the collection efficiency of MSB. The model is verified by experiments. | Gui, KT Zhang, H Shi, MH Xu, YQ | 2001 | Journal of Environmental Sciences2001，13，4： | 3 |
| 4 | Excitatoryamino acids and glaucoma 显示文摘 | Shi JM Jing YQ | 2004 | Int J Ophthalmol2004，4，4： | 1 |
| 5 | Serum biomarker of diabetic pe- ripheral neuropathy indentified by differential proteomics显示文摘 | Tang W Shi YQ Zou JJ | 2011 | Front Biosei2011，16，： | 1 |
| 6 | Endovascular therapy for stanford type B aortic dissection in 102cases显示文摘 | Fu WG Shi Y Wang YQ | 2005 | Asian Surg2005，28，4： | 1 |
| 7 | Reversible data hiding显示文摘 | Ni ZC Shi YQ Ansari N | 2006 | IEEE Transactions on Circuits and Systems for Video Technology2006，16，3： | 1 |
| 8 | In vitro propagation of spermatogonial stem cells from KM mice 显示文摘 | Shi YQ Wang QZ Liao SY | 2006 | Front Biosci2006，11，： | 1 |
| 9 | Micrometastases of solitary hepatocellular carcinoma and appropriate resection margin显示文摘 | Shi M Zhang CQ Zhang YQ | 2004 | World J Surg2004，28，4： | 1 |
| 10 | Association of the CT- LA24 gene with rheumatoid arthritis in Chinese Han popula- tion显示文摘 | Cai L Zhang DQ Shi YQ | 2005 | EurJ HumGenet2005，13，： | 1 |
| 11 | The analysis of status and clinical implication of KIT and PDGFRA mutations in gastrointestinal stromal tumor (GIST)显示文摘 | DU CY Shi YQ Zhou Y | 2008 | J Surg Oneol2008，98，3： | 1 |
| 12 | Effects of gender and estradiol treatment on focal brain ischemia显示文摘 | Zhang YQ Shi J Rajakumar G | 1998 | Brain Res1998，784，12： | 1 |
| 13 | Incidence of Deep SternalWound Infection in Diabetic Patients Undergoing Off-Pump Skeletonized Internal Thoracic Artery Grafting 显示文摘 | Ding WJ Ji Q Shi YQ | 2015 | Cardiology2015，133，2： | 1 |
| 14 | Visceral hypersensitivity following cold water intake in subjects with irritable bowel syndrome显示文摘 | Zuo XL Li YQ Shi L | 2006 | J Gastroenterol2006，41，4： | 1 |
| 15 | Microsurgecal treatment of intracranial arteriovenous malformations 显示文摘 | Chen XC Shi YQ Wu JS | 2003 | Chin J Clin Neruosurg2003，5，1： | 1 |
| 16 | Differential display of vincristineresistance-related genes in gastric cancer SGC7901 cell 显示文摘 | Wang X Lan M Shi YQ | 2002 | World J Gastroenterol2002，8，1： | 1 |
| 17 | High nuclear expression of STAT3 is associated with unfavorable prognosis in diffuse large Bcell lymphoma显示文摘 | Wu ZL Song YQ Shi YF | 2011 | J Hematol Oncol2011，4，1： | 1 |
| 18 | Effects of 17 beta-estradiol on glucose transporter 1 expression and endothelial cell survival following focal ischemia in the rats显示文摘 | Zhang YQ Simpkins JW | 1997 | Exp Brain Res1997，117，： | 1 |
| 19 | Dialectic standard research about sports insomnia induced by tired qi injury 显示文摘 | Shi YQ Zhu KJ Zhou ZH | 2004 | Chin J Sports Med2004，23，6： | 1 |
| 20 | Excitatoryamino acids and glaucoma 显示文摘 | Shi JM Jing YQ | 2004 | Int J Ophthalmol2004，4，4： | 1 |
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