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| 1 | YB-1 stabilizes HIV-1 genomic RNA and enhances viral production显示文摘HIV-1 utilizes cellular factors for effi cient replication.The viral RNA is different from cellular mRNAs in many aspects,and is prone to attacks by cellular RNA quality control systems.To establish effective infection,the virus has evolved multiple mechanisms to protect its RNA.Here,we show that expression of the Y-box binding protein 1(YB-1)enhanced the production of HIV-1.Downregulation of endogenous YB-1 in producer cells decreased viral production.YB-1 increased viral protein expression by stabilizing HIV-1 RNAs.The stem loop 2 in the HIV-1 RNA packaging signal was mapped to be the YB-1-responsive element.Taken together,these results indicate that YB-1 stabilizes HIV-1 genomic RNA and thereby enhances HIV-1 gene expression and viral production. | Xin Mu Wei Li Xinlu Wang Guangxia Gao | 2013 | Protein & Cell2013，4，8： | 2 |
| 2 | Analyses of SELEX-derived ZAP-binding RNA aptamers suggest that the binding specificity is determined by both structure and sequence of the RNA显示文摘The zinc-finger antiviral protein(ZAP)is a host factor that specifically inhibits the replication of certain viruses,including murine leukemia virus,Sindbis virus and Ebola virus,by targeting the viral mRNAs for degradation.ZAP directly binds to the target viral mRNA and recruits the cellular RNA degradation machinery to degrade the RNA.No significant sequence similarity or obvious common motifs have been found in the so far identified target viral mRNAs.The minimum length of the target sequence is about 500 nt long.Short workable ZAP-binding RNAs should facilitate further studies on the ZAP-RNA interaction and characterization of such RNAs may provide some insights into the underlying mechanism.In this study,we used the SELEX method to isolate ZAP-binding RNA aptamers.After 21 rounds of selection,ZAP-binding aptamers were isolated.Sequence analysis revealed that they are G-rich RNAs with predicted stem-loop structures containing conserved“GGGUGG”and“GAGGG”motifs in the loop region.Insertion of the aptamer sequence into a luciferase reporter failed to render the reporter sensitive to ZAP.However,overexpression of the aptamers modestly but significantly reduced ZAP’s antiviral activity.Substitution of the conserved motifs of the aptamers significantly impaired their ZAP-binding ability and ZAP-antagonizing activity,suggesting that the RNA sequence is important for specific interaction between ZAP and the target RNA.The aptamers identified in this report should provide useful tools to further investigate the details of the interaction between ZAP and the target RNAs. | Zhi Huang Xinlu Wang Guangxia Gao | 2010 | Protein & Cell2010，1，8： | 2 |
| 3 | Two New Error-correcting Pooling Designs from d-bounded Distance-regular Graphs 显示文摘 | ZHANG Xinlu GUO J un GAO Suogang | 2009 | Journal of Combinatorial Optimization2009，17，： | 1 |
| 4 | Expression of YB-1 enhances production of murine leukemia virus vectors by stabilizing genomic viral RNA显示文摘Murine leukemia virus(MLV)-based retroviral vectors is widely used for gene transfer and basic research,and production of high-titer retroviral vectors is very important.Here we report that expression of the Y-box binding protein 1(YB-1)enhanced the production of infectious MLV vectors.YB-1 specifically increased the stability of viral genomic RNA in virus-producing cells,and thus increasing viral RNA levels in both producer cells and virion particles.The viral element responsive to YB-1 was mapped to the repeat sequence(R region)in MLV genomic RNA.These results identified YB-1 as a MLV mRNA stabilizer,which can be used for improving production of MLV vectors. | Wei Li Xinlu Wang Guangxia Gao | 2012 | Protein & Cell2012，3，12： | 1 |
| 5 | Soil nutrient content and nutrient balances in newly-built solar greenhouses in northern China 显示文摘 | GAO Jiajia BAI Xinlu ZHOU Be | 2012 | Nutrient Cycling in Agroecosystems2012，94，1： | 1 |
| 6 | Two New Error-correcting Pooling Designs from d-bounded Distance-regular Graphs 显示文摘 | ZHANG Xinlu GUO J un GAO Suogang | 2009 | Journal of Combinatorial Optimization2009，17，： | 1 |
| 7 | LncRNA ZFAS1 regulates cardiomyocyte differentiation of human embryonic stem cells显示文摘Background:Cardiomyocytes derived from human embryonic stem cells(hESCs)are regulated by complex and stringent gene networks during differentiation.Long non-coding RNAs(lncRNAs)exert critical epigenetic regulatory functions in multiple differentiation processes.However,the involvement of lncRNAs in the differentiation of hESCs into cardiomyocytes has not yet been fully elucidated.Here,we identified the key roles of ZFAS1(lncRNA zinc finger antisense 1)in the differentiation of cardiomyocytes from hESCs.Methods:A model of cardiomyocyte differentiation from stem cells was established using the monolayer differentiation method,and the number of beating hESCs-derived cardiomyocytes was calculated.Gene expression was analyzed by quantitative real-time PCR(qRTPCR).Immunofluorescence assays were performed to assess the expression of cardiac troponin T(cTnT)andα-actinin protein in cardiomyocytes.Results:qRT-PCR showed that ZFAS1 expression in the mesoderm was significantly higher than that in embryonic stem cells,cardiac progenitor cells,and cardiomyocytes.Knockdown of ZFAS1 inhibited cardiomyocyte differentiation from hESCs,which was characterized by reduced expression of the cardiac-specific markers cTnT,α-actinin,myosin heavy chain 6(MYH6),and myosin heavy chain 7(MYH7).In contrast,ZFAS1 overexpression remarkably increased the percentage of spontaneously beating cardiomyocytes.In terms of the mechanism,we found that ZFAS1 is an antisense lncRNA at the 5′end of the protein-coding gene ZNFX1.Knockdown of ZFAS1 could increase the mRNA expression level of ZNFX1.Furthermore,qRT-PCR demonstrated that the silencing of ZNFX1 led to an increase in cardiac-specific markers that predicted the promotion of cardiomyocyte differentiation.Conclusion:Altogether,these data suggest that lncRNA-ZFAS1 is required for cardiac differentiation by functionally inhibiting the expression of ZNFX1,which may provide a reference for the treatment of heart disease to a certain extent. | YANG CAO YINING LIU YANG YU XIAOFEI GUO XIUXIU WANG WENYA MA HANJING LI ZHONGYU REN XINLU GAO SIJIA LI HAOYU JI HONGYANG CHEN HONG YAN YANAN TIAN XIN WANG BENZHI CAI | 2023 | BIOCELL2023，47，6： | 0 |
| 8 | Identification of new type I interferon- stimulated genes and investigation of their involvement in IFN-β activation显示文摘 | Xiaolin Zhang Wei Yang Xinlu Wang Xuyuan Zhang Huabin Tian Hongyu Deng Liguo Zhang Guangxia Gao | 2018 | Protein & Cell2018，9，9： | 0 |
| 9 | Mitochondria are an important target of photobiomodulation in cardiomyocytes显示文摘Photobiomodulation(PBM)has been shown to delay the pathological process of heart failure,but the exact mechanism of action is not clear.Mitochondria occupy one-third of the volume of mammalian cardiomyocytes(CMs)and are central transport stations for CM energy metabolism.Therefore,in this study,we explored the regulatory effects of 630 nm light-emitting diodes(LED-Red)on the mitochondria of CMs.The results show that LED-Red-based PBM promotes adenosine triphosphate(ATP)synthesis by upregulating the expression of glycolipid metabolizing enzymes.Correspondingly,there was an improvement in the activity of succinate dehydrogenase(SDH),a key enzyme in the mitochondrial electron transport chain,and the mitochondrial membrane potential.Meanwhile,LED-Red affected the state of mitochondrial oxidative stress and promoted the generation of reactive oxygen species(ROS),but the increased ROS production did not damage the CMs.In addition,mitochondrial division and fusion were also affected by the stimulation of LED-Red.Finally,PBM treatment led to a significant increase in transcript levels of mitochondrial transcription factor A(TFAM),which controls the stability of the mitochondrial genome.Collectively,irradiation with LEDs at 630 nm played a regulatory role in mitochondrial function,suggesting that mitochondria appear to be the recipients of PBM treatment.This study provides more insights into the mechanisms underlying PBM treatment in heart diseases. | XINLU GAO XIUXIU WANG WENWEN ZHANG HANJING LI FAN YANG WENYA MA YU LIU | 2022 | BIOCELL2022，46，12： | 0 |
| 10 | Cyclin L1 controls cardiomyocyte proliferation and heart repair after injury显示文摘Dear Editor,Myocardial infarction(MI)is characterized by the loss of functional cardiomyocyte(CM)in the heart,resulting in cardiac systolic dysfunction and heart failure.1,2 Increasing evidence suggested that in the heart of neonatal mice after apical resection(AR),the CM can proliferate and regenerate myocardium to repair the heart.While in the heart of adult mice after MI,the CM loses the ability to re-enter the cell cycle but undergoes hypertrophic growth. | Rui Gong Xinlu Gao Yu Liu Yifu Shen Zuke Jiang Xiuxiu Wang Naufal Zagidullin Wenya Ma Ning Wang Benzhi Cai | 2023 | Signal Transduction and Targeted Therapy2023，8，7： | 0 |
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