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1Erianin,a novel dibenzyl compound in Dendrobium extract,inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis显示文摘Ferroptosis,a novel form of programmed cell death,is characterized by iron-dependent lipid peroxidation and has been shown to be involved in multiple diseases,including cancer.Stimulating ferroptosis in cancer cells may be a potential strategy for cancer therapy.Therefore,ferroptosis-inducing drugs are attracting more attention for cancer treatment.Here,we showed that erianin,a natural product isolated from Dendrobium chrysotoxum Lindl,exerted its anticancer activity by inducing cell death and inhibiting cell migration in lung cancer cells.Subsequently,we demonstrated for the first time that erianin induced ferroptotic cell death in lung cancer cells,which was accompanied by ROS accumulation,lipid peroxidation,and GSH depletion.The ferroptosis inhibitors Fer-1 and Lip-1 but not Z-VAD-FMK,CQ,or necrostatin-1 rescued erianin-induced cell death,indicating that ferroptosis contributed to erianin-induced cell death.Furthermore,we demonstrated that Ca^(2+)/CaM signaling was a critical mediator of erianin-induced ferroptosis and that blockade of this signaling significantly rescued cell death induced by erianin treatment by suppressing ferroptosis.Taken together,our data suggest that the natural product erianin exerts its anticancer effects by inducing Ca^(2+)/CaMdependent ferroptosis and inhibiting cell migration,and erianin will hopefully serve as a prospective compound for lung cancer treatment.Peng Chen Qibiao Wu Jiao Feng Lili Yan Yitian Sun Shuiping Liu Yu Xiang Mingming Zhang Ting Pan Xiaying Chen Ting Duan Lijuan Zhai Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Xuemeng Han Yicong Li Liuxi Chen Ying Liu Xingxing Huang Ting Jin Wenzheng Zhang Hong Luo Xiaohui Chen Yongqiang Li Qiujie Li Guohua Li Qin Zhang Lvjia Zhuo Zuyi Yang Huifen Tang Tian Xie Xiaoping Ouyang Xinbing Sui 2020Signal Transduction and Targeted Therapy2020,5,1:42
2An assessment of summer sensible heat flux on the Tibetan Plateau from eight data sets显示文摘The eight datasets of the summer (June-August) surface sensible heat (SH) flux over the Tibetan Plateau (TP) are compared on the time scales of the climatology,interannual variability and linear trend during 1980-2006.These data sets include five reanalyses (National Center for Environmental Prediction reanalysis,NCEPR1 and NCEPR2,NCEP climate forecast system reanalysis,CFSR,Japanese 25-year reanalysis,JRA,and European Centre for Medium Range Weather Forecasts reanalysis,ERA40),two land surface model outputs (Noah model data of Global Land Data Assimilation System version 2,G2_Noah,and Simple Biosphere version 2 output by Yang et al.,YSiB2),and estimated SH based on China Meteorological Administration (CMA) station observations,ObCh.The results suggest that the summer SH on the TP differs from one dataset to another due to different inputs and calculations.Climatologically,the ERA40 and JRA distribute rather uniformly while the other six products show similar regional disparities,that is,larger in the west than in the east and stronger in the north and the south than in the middle of the plateau.The mean magnitude of the SH averaged over the 76 stations above the TP varies considerably among each dataset with the difference of more than 20 W m?2 between the maximum (G2_Noah) and minimum (ObCh).Nevertheless,they are consistent in the interannual variability and mostly show a significant decreasing trend corresponding to the weakening surface wind speed,in spite of the distinct trend for the ground-air temperature difference among the different data sets.These two consistencies indicate the particular availability of the SH products,which is helpful to the relevant climate dynamics research.ZHU XiaYing LIU YiMin WU GuoXiong 2012Science China Earth Sciences2012,55,5:24
3Baicalin induces ferroptosis in bladder cancer cells by downregulating FTH1显示文摘Ferroptosis is a non-apoptotic regulated cell death caused by iron accumulation and subsequent lipid peroxidation.Currently,the therapeutic role of ferroptosis on cancer is gaining increasing interest.Baicalin an active component in Scutellaria baicalensis Georgi with anticancer potential various cancer types;however,the effects of baicalein on bladder cancer and the underlying molecular mechanisms remain largely unknown.In the study,we investigated the effect of baicalin on bladder cancer cells5637 and KU-19-19.As a result,we show baicalin exerted its anticancer activity by inducing apoptosis and cell death in bladder cancer cells.Subsequently,we for the first time demonstrate baicalin-induced ferroptotic cell death in vitro and in vivo,accompanied by reactive oxygen species(ROS) accumulation and intracellular chelate iron enrichment.The ferroptosis inhibitor deferoxamine but not necrostatin-1,chloroquine(CQ),N-acetyl-L-cysteine,L-glutathione reduced,or carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone(Z-VAD-FMK) rescued baicalin-induced cell death,indicating ferroptosis contributed to baicalin-induced cell death.Mechanistically,we show that ferritin heavy chain1(FTH1) was a key determinant for baicalin-induced ferroptosis.Overexpression of FTH1 abrogated the anticancer effects of baicalin in both 5637 and KU19-19 cells.Taken together,our data for the first time suggest that the natural product baicalin exerts its anticancer activity by inducing FTH1-dependent ferroptosis,which will hopefully provide a prospective compound for bladder cancer treatment.Na Kong Xiaying Chen Jiao Feng Ting Duan Shuiping Liu Xueni Sun Peng Chen Ting Pan Lili Yan Ting Jin Yu Xiang Quan Gao Chengyong Wen Weirui Ma Wencheng Liu Mingming Zhang Zuyi Yang Wengang Wang Ruonan Zhang Bi Chen Tian Xie Xinbing Sui Wei Tao 2021Acta Pharmaceutica Sinica B2021,11,12:23
4Meridional seesaw-like distribution of the Meiyu rainfall over the Changjiang-Huaihe River Valley and characteristics in the anomalous climate years显示文摘Although Meiyu rainfall has its in-phase spatial variability over the Changjiang-Huaihe River Valley (CHRV) in most years, it is distributed in some years like a seesaw to the north and south of the Changjiang River, when the precipitation tends to be nearly normal throughout the valley, which would inevitably increase difficulties of making short-term prediction of the rainfall. For this reason, EOF analysis is made on 15 related stations’ precipitation from June to July during 1951─2004, revealing that the EOF2 mode shows largely a north-south seesaw-like pattern, and thereby classifying Meiyu patterns into two types: 'northern drought and southern flood (NDSF)' and 'northern flood and southern drought (NFSD)'. Afterwards, the authors investigated ocean-atmospheric characteristics when these two anomalous types occured using the NCEP reanalysis (version 1) and the extended reconstructed SSTs (version 2). The results show that in the NDSF years, the low-level frontal area and moisture convergence center lie more southward, accompanied by weaker subtropical summer mon- soon over East Asia, with the western Pacific subtropical high and 200 hPa South Asia High being more southward. Both the Northern and Southern Hemisphere Annular Modes are stronger than normal in preceding February; SST is higher off China during boreal winter and spring and the opposite happens in the NFSD years. Also, this seesaw-form Meiyu rainfall distribution might be affected to some degree by the previous ENSO event.ZHU XiaYing HE JinHai WU ZhiWei 2007Chinese Science Bulletin2007,52,17:12
5Characterization and phylogenetic analysis of a phenanthrene-degrading strain isolated from oil-contaminated soil显示文摘Bacterium strain EVA17 was isolated from an oil-contaminated soil, and identified as Sphingomonas sp. based on analysis of 16S rDNA sequence,cellular fatty acid composition and physiological-chemical tests. The salicylate hydroxylase and catechol 2,3-dioxygenase(C23O) were detected in cell-free lysates, suggesting a pathway for phenanthrene catabolism via salicylate and catechol. Alignment showed that both of the C23O and GST genes of the strain EVA17 had high similarity with homologues of strains from genus Sphingomonas. The phylogenetic analysis based on 16S rDNA and C23O gene sequence indicated that EVA17 should be classified into genus Sphingomonas, although the two phylogenetic trees were slightly different from each other. The results of co-amplification and sequence determination indicated that GST gene should be located upstream of the C23O gene.XIAYing MINHang LUZhen-mei YEYang-fang 2004Journal of Environmental Sciences2004,16,4:7
6Preparation, characterization, pharmacokinetics and anticancer effects of PEGylated β-elemene liposomes显示文摘Objective:This study aimed to develop a new polyethylene glycol(PEG)ylatedβ-elemene liposome(PEG-Lipo-β-E)and evaluate its characterization,pharmacokinetics,antitumor effects and safety in vitro and in vivo.Methods:The liposomes were prepared by ethanol injection and high-pressure micro-jet homogenization.Characterization of the liposomes was conducted,and drug content,entrapment efficiency(EE),in vitro release and stability were studied by ultra-fast liquid chromatography(UFLC)and a liquid surface method.Blood was drawn from rats to establish the pharmacokinetic parameters.The anticancer effect was evaluated in a KU-19-19 bladder cancer xenograft model.Histological analyses were performed to evaluate safety.Results:The PEG-Lipo-β-E showed good stability and was characterized as 83.31±0.181 nm in size,0.279±0.004 in polydispersity index(PDI),-21.4±1.06 mV in zeta potential,6.65±0.02 in pH,5.024±0.107 mg/mL inβ-elemene(β-E)content,and 95.53±1.712%in average EE.The Fourier transform infrared spectroscopy(FTIR)and differential scanning calorimetry(DSC)indicated the formation of PEG-Lipo-β-E.Compared to elemene injection,PEG-Lipo-β-E demonstrated a 1.75-fold decrease in clearance,a 1.62-fold increase in half-life,and a 1.76-fold increase in area under the concentration-time curves(AUCs)from 0 hour to 1.5 hours(P<0.05).PEG-Lipo-β-E also showed an enhanced anticancer effect in vivo.Histological analyses showed that there was no evidence of toxicity to the heart,kidney,liver,lung or spleen.Conclusions:The present study demonstrates PEG-Lipo-β-E as a new formulation with ease of preparation,high EE,good stability,improved bioavailability and antitumor effects.Bingtao Zhai Qibiao Wu Wengang Wang Mingming Zhang Xuemeng Han Qiujie Li Peng Chen Xiaying Chen Xingxing Huang Guohua Li Qin Zhang Ruonan Zhang Yu Xiang Shuiping Liu Ting Duan Jianshu Lou Tian Xie Xinbing Sui 2020Cancer Biology & Medicine2020,17,1:6
7An ATF24 peptide-functionalized β-elemene-nanostructured lipid carrier combined with cisplatin for bladder cancer treatment显示文摘Objective:In this study,we aimed to develop an amino-terminal fragment(ATF)peptide-targeted liposome carryingβ-elemene(ATF24-PEG-Lipo-β-E)for targeted delivery into urokinase plasminogen activator receptor-overexpressing bladder cancer cells combined with cisplatin(DDP)for bladder cancer treatment.Methods:The liposomes were prepared by ethanol injection and high-pressure microjet homogenization.The liposomes were characterized,and the drug content,entrapment efficiency,andin vitro release were studied.The targeting efficiency was investigated using confocal microscopy,ultra-fast liquid chromatography,and an orthotopic bladder cancer model.The effects of ATF24-PEG-Lipo-β-E combined with DDP on cell viability and proliferation were evaluated by a Cell Counting Kit-8(CCK-8)assay,a colony formation assay,and cell apoptosis and cell cycle analyses.The anticancer effects were evaluated in a KU-19-19 bladder cancer xenograft model.Results:ATF24-PEG-Lipo-β-E had small and uniform sizes(~79 nm),high drug loading capacity(~5.24 mg/mL),high entrapment efficiency(98.37±0.95%),and exhibited sustained drug release behavior.ATF24-PEG-Lipo-β-E had better targeting efficiency and higher cytotoxicity than polyethylene glycol(PEG)ylatedβ-elemene liposomes(PEG-Lipo-β-E).DDP,combined with ATF24-PEG-Lipo-β-E,exerted a synergistic effect on cellular apoptosis and cell arrest at the G2/M phase,and these effects were dependent on the caspase-dependent pathway and Cdc25C/Cdc2/cyclin B1 pathways.Furthermore,thein vivo antitumor activity showed that the targeted liposomes effectively inhibited the growth of tumors,using the combined strategy.Conclusions:The present study provided an effective strategy for the targeted delivery ofβ-elemene(β-E)to bladder cancer,and a combined strategy for bladder cancer treatment.Bingtao Zhai Peng Chen Wengang Wang Shuiping Liu Jiao Feng Ting Duan Yu Xiang Ruonan Zhang Mingming Zhang Xuemeng Han Xiaying Chen Qiujie Li Guohua Li Ying Liu Xingxing Huang Wenzheng Zhang Ting Pan Lili Yan Ting Jin Tian Xie Xinbing Sui 2020Cancer Biology & Medicine2020,17,3:5
8A method to model the effect of pre-existing cracks on P-wave velocity in rocks显示文摘Crack closure is one of the reasons inducing changes of P-wave velocity of rocks under compression.In this context,a method is proposed to investigate the relationships among P-wave velocity,pre-existing cracks,and confining pressure based on the discrete element method(DEM).Pre-existing open cracks inside the rocks are generated by the initial gap of the flat-joint model.The validity of the method is evaluated by comparing the P-wave velocity tested on a sandstone specimen with numerical result.As the crack size is determined by the diameter of particles,the effects of three factors,i.e.number,aspect ratio,and orientation of cracks on the P-wave velocity are discussed.The results show that P-wave velocity is controlled by the(i.e.number) of open micro-cracks,while the closure pressure is determined by the aspect ratio of crack.The reason accounting for the anisotropy of P-wave velocity is the difference in crack number in measurement paths.Both of the number and aspect ratio of cracks can affect the responses of P-wave velocity to the applied confining pressure.Under confining pressure,the number of open cracks inside rocks will dominate the lowest P-wave velocity,and the P-wave velocity of the rock containing narrower cracks is more sensitive to the confining pressure.In this sense,crack density is difficult to be back-calculated merely by P-wave velocity.The proposed method offers a means to analyze the effect of pre-existing cracks on P-wave velocity.Haimeng Shen Xiaying Li Qi Li Haibin Wang 2020Journal of Rock Mechanics and Geotechnical Engineering2020,12,3:5
9氮沉降模拟实验亟待研究大气氨浓度增加的影响显示文摘大气活性氮污染及其过量沉降是全球变化重要议题。为研究氮沉降对自然生态系统结构和功能的影响,基于喷淋加氮的控制实验在草地或林下广泛开展。该实验体系假定氮沉降是通过增加土壤氮的有效性从而影响整个生态系统,没有考虑植被与大气活性氮的相互作用,也未能反映氮沉降以干沉降为主的自然过程。考虑到大气氨是氮沉降的主要前体物,且全球氨气浓度呈现上升趋势,未来的氮沉降模拟实验亟待研究氨气干沉降的重要影响,以期更加完整的刻画大气氮沉降过程及其生态环境效应。PAN Yuepeng TIAN Shili WU Dianming XU Wen ZHU Xiaying LIU Chunyan LI Dejun FANG Yunting DUAN Lei LIU Xuejun WANG Yuesi 2020Atmospheric and Oceanic Science Letters2020,13,3:5
10PCDH17 increases the sensitivity of colorectal cancer to 5-fluorouracil treatment by inducing apoptosis and autophagic cell death显示文摘5-Fluorouracil(5-FU)is known as a first-line chemotherapeutic agent against colorectal cancer(CRC),but drug resistance occurs frequently and significantly limits its clinical success.Our previous study showed that the protocadherin 17(PCDH17)gene was frequently methylated and functioned as a tumor suppressor in CRC.However,the relationship between PCDH17 and 5-FU resistance in CRC remains unclear.Here,we revealed that PCDH17 was more highly expressed in 5-FU-sensitive CRC tissues than in 5-FU-resistant CRC tissues,and high expression of PCDH17 was correlated with high BECN1 expression.Moreover,this expression profile contributed to superior prognosis and increased survival in CRC patients.Restoring PCDH17 expression augmented the 5-FU sensitivity of CRC in vitro and in vivo by promoting apoptosis and autophagic cell death.Furthermore,autophagy played a dominant role in PCDH17-induced cell death,as an autophagy inhibitor blocked cell death to a greater extent than the pancaspase inhibitor Z-VAD-FMK.PCDH17 inhibition by siRNA decreased the autophagy response and 5-FU sensitivity.Mechanistically,we showed that c-Jun NH2-terminal kinase(JNK)activation was a key determinant in PCDH17-induced autophagy.The compound SP600125,an inhibitor of JNK,suppressed autophagy and 5-FU-induced cell death in PCDH17-reexpressing CRC cells.Taken together,our findings suggest for the first time that PCDH17 increases the sensitivity of CRC to 5-FU treatment by inducing apoptosis and JNK-dependent autophagic cell death.PCDH17 may be a potential prognostic marker for predicting 5-FU sensitivity in CRC patients.Shuiping Liu Haoming Lin Da Wang Qiang Li Hong Luo Guoxiong Li Xiaohui Chen Yongqiang Li Peng Chen Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Mingming Zhang Xuemeng Han Qiujie Li Liuxi Chen Ying Liu Xiaying Chen Guohua Li Yu Xiang Ting Duan Jiao Feng Jianshu Lou Xingxing Huang Qin Zhang Ting Pan Lili Yan Ting Jin Wenzheng Zhang Lvjia Zhuo Yitian Sun Tian Xie Xinbing Sui 2019Signal Transduction and Targeted Therapy2019,4,1:4
11Copy number and zygosity determination of transgenic rapeseed by droplet digital PCR显示文摘Establishing an accurate and rapid method for copy number and zygosity determination can accelerate genetic engineering research process. In this study, droplet digital PCR (ddPCR), an emerging DNA absolute quantification technology, was used to identify single-copy homozygous transgenic lines from a batch of T0 transgenic rapeseed harboring 11 exogenous elements. Copy number of exogenous gene was evaluated in T0 generation based on calculated ratio between transgene and reference CruA gene, single-copy transformants were selected for selfing followed by subsequent zygosity analysis. Single-copy homozygous transgenic plants were successfully screened out in T1 generation by ddPCR.Segregation analysis with T2 seedlings verified that identification results of ddPCR were accurate and reliable. This study provides a novel rapid and accurate method for copy number and zygosity determination in transgenic rapeseed which overcomes disadvantages of traditional Southern analysis and recently developed real-time quantitative method.Yuhua Wu Jun Li Xiaying Li Jingang Liang Yunjing Li Xinhua Zeng Gang Wu 2017Oil Crop Science2017,2,2:4
12Three-dimensional large-aperture lens antennas with gradient refractive index显示文摘We propose an accurate method for designing three-dimensional(3D)large-aperture metamaterial slab lens antennas with gradient refractive index(GRIN).According to the geometric optics,Fermat principle,ray-tracing technique and impedance matching,the 3D GRIN slab lenses with large apertures are accurately designed and simulated.With the aid of the efective medium theory,an X-band and a Ku-band conical horn antennas loaded with the 3D GRIN slab lenses of 250-mm diameter are experimentally realized using the drilling-hole technique on the printed circuit boards(PCBs)as the unit cells of metamaterials.Compared to the traditional dielectric lens with the same aperture,the proposed antennas have very good performance with high directivity,and the gain is increased by 2 to 5 dB.Using the same method,we design and realize a huge-aperture GRIN lens in the X band with a diameter of 1000 mm,which is composed of nearly one millions of inhomogeneous unit cells of square-ring resonators and dielectric blocks with drilling holes.Due to the huge aperture size,the electromagnetic ray paths inside and outside of the GRIN lens are verifed and optimized using the ray tracing technique.Measurement results show good performance of the proposed antenna with high directivity.ZHOU XiaoYang ZOU XiaYing YANG Yan MA HuiFeng CUI TieJun 2013Science China(Information Sciences)2013,56,12:3
13塔里木盆地碳酸盐岩储层酸性气体注入的数值模拟及可行性评价显示文摘塔里木盆地油气藏开采过程中产生了大量的酸性气体,其中包括CO2和H2S。安全处置酸性气体需要适合的管理计划,普遍策略是将其注入深层地层——这样可减少温室气体排放,避免脱硫产生的成本。注酸气的可行性评价需要对其潜在的物理和地球化学影响进行详细的调查。通过塔里木盆地碳酸盐岩地层的矿物组成和水化学特征,对酸性气体与矿物基质和地层水的反应运移进行了模拟研究,确定了酸性气体与矿物基质和地层水的物理和地球化学相互作用。以19200 m^3/d(20℃,1.013×10^5 Pa)的恒定速率注入酸性气体(含59%的CO2和41%的H2S)25年,使用TOUGHREACT代码的TMVR_EOSG模块进行模拟,模拟时间为1万年。结果表明,最小含水饱和度远大于残余含水饱和度,压力累积值低于允许压力增加值。Xiaoyan Zhang Qi Li Liange Zheng Xiaying Li and Liang Xu 陆倩 2020油气田地面工程2020,39,6:2
14Synergism of hydroxyapatite nanoparticles and recombinant mutant human tumour necrosis factor‐α in chemotherapy of multidrug‐resistant hepatocellular carcinoma显示文摘GaopengLi ShengliDong JunyuanQu ZhengangSun ZhiyongHuang LuYe HuifangLiang XiAi WanguangZhang XiaopingChen 2010Liver International2010,,4:2
15PolymorphicCAGrepeatintheandrogenreceptorgeneinpolycysticovarysyndromepatients显示文摘XiaY CheY ZhangX etal 2012MolMedRep2012,5,5:1
16miR-204functionsasatumorsuppres-sorbyregulatingSIX1inNSCLC显示文摘XiaY ZhuY MaT etal 2014FEBSLett2014,588,20:1
17A Conflict Detection Scheme for Concurrency Control of R-tree显示文摘Despite extensive research on R-trees, most of the proposed schemes have not been integrated into existing DBMS owing to the lack of protocols of concurrency control. R-link tree is an acceptable data structure to deal with this issue, but problems like phantom still exist. In this paper, we focus on a conflict detection scheme based on R-link tree for complete concurrency control. An in-memory operation control list is designed to suspend conflicting operations. The main features of this approach are (1) it can be implemented easily and does not need any extra information; (2) No deadlocks are involved in locking scheme; (3) Non-conflicting operations are not restricted; and (4) Phantom problems in R-link tree are avoided through beforehand predication. The experiment results show that this scheme is correct and gains better system performance.XIAYing PENGDa-qin 2003The Journal of China Universities of Posts and Telecommunications2003,10,1:1
18Capacity fading on cycling of4V Li/LiMn2O4 cells 显示文摘Y Xiay Zhou Yoshio M 1997J Electrochem Soc1997,144,:1
19Hydromechanical behaviors of andesite under different stress states during fluid injection显示文摘Water reinjection into the formation is an indispensable operation in many energy engineering practices.This operation involves a complex hydromechanical(HM)coupling process and sometimes even causes unpredictable disasters,such as induced seismicity.It is acknowledged that the relative magnitude and direction of the principal stresses significantly influence the HM behaviors of rocks during injection.However,due to the limitations of current testing techniques,it is still difficult to comprehensively conduct laboratory injection tests under various stress conditions,such as in triaxial extension stress states.To this end,a numerical study of HM changes in rocks during injection under different stress states is conducted.In this model,the saturated rock is first loaded to the target stress state under drainage conditions,and then the stress state is maintained and water is injected from the top to simulate the formation injection operation.Particular attention is given to the difference in HM changes under triaxial compression and extension stresses.This includes the differences in the pore pressure propagation,mean effective stress,volumetric strain,and stress-induced permeability.The numerical results demonstrate that the differential stress will significantly affect the HM behaviors of rocks,but the degree of influence is different under the two triaxial stress states.The HM changes caused by the triaxial compression stress states are generally greater than those of extension,but the differences decrease with increasing differential stress,indicating that the increase in the differential stress will weaken the impact of the stress state on the HM response.In addition,the shear failure potential of fracture planes with various inclination angles is analyzed and summarized under different stress states.It is recommended that engineers could design suitable injection schemes according to different tectonic stress fields versus fault occurrence to reduce the risk of injection-induced seismicity.Miao He Qi Li Xiaying Li Liang Xu Michael Kühn 2021Journal of Rock Mechanics and Geotechnical Engineering2021,13,4:1
20Chemically modified chitosan beads as matrices for adsorptive separation of proteins by molecularly imprinted polymer显示文摘GUO T XIAY HAO G 2005Carbohydrate Polymers2005,62,3:1
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