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1篇 您的检索式：作者名="Xiangan Long"

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		题名	作者	年代	出处	被引量
	1	A digital microfluidic single-cell manipulation systemoptimized by extending-depth-of-field device显示文摘Microfuidic systems have been widely utilized in high-throughput biology analysis,but thedificulties in iquid manipulation and cell cultivation limit its application.This work has developed a new digital microfluidic(DMF)system for on-demand droplet control.By adopting anextending-depth-of-field(EDoF)phase modulator to the optical system,the entire depth of themicrofluidic channel can be covered in one image without any refocusing process,ensuring that 95%of the particles in the droplet are captured within three shots together with shaking pro-cesses.With this system,suspension droplets are generated and droplets containing only oneyeast cll can be recognized,then each single cell is cultured in the array of the chip.Byobservingtheir growth in cell numbers and the green fluorescence protein(GFP)production via fluorescence imaging,the single cell with the highest production can be identified.The results haveproved the heterogeneity of yeast cells,and showed that the combined system can be applied forrapid single-cell sorting,cultivation,and analysis.	Qiushu Chen Qi Meng Yuzhe Liu Xiangan Long Yawei Kong Longfang Yao Liwen Chen Chuanyong Wu Kaiqin Chu Lan Mi Jiong Ma	2023	Journal of Innovative Optical Health Sciences2023，16，3：	0

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