5篇
您的检索式：作者名="XiaopingYang"
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| 1 | The Software Costs Estimation Based on UML Model显示文摘UML is a standard modeling language used in object-oriented analysis and design. Function point analysis is a method used to measure the size of an application, It is independent of the implementation programming language. Its measuring result can be compared between different development processes. This paper presents a method to use the requirements analysis model of UML to analysis the application's function points, so software developer can use it to estimate the project's size and cost. An improved method is given at the end of this paper. | XiaopingYang LuJun YuefengZhao | 2004 | Journal of Systems Science and Information2004，2，4： | 1 |
| 2 | Hepatitis B virus S gene enhances immune responses of a multiple-epitope DNA construct of hepatitis C virus in mice显示文摘The purpose of this study was to construct an eukaryotic DNA vector encoding a multiple-epitope antigen (MFC) of hepatitis C virus (HCV) and a hepatitis B surface antigen (HBsAg) , and explore the effect of HBsAg gene on the immunity of HCV multiple-epitope DNA construct in vitro and in vivo in mice. An HCV DNA vector (pVAX1-HBs-MFC) was constructed by fusing HBsAg gene to the N-terminal of an HCV multiple-epitope antigen gene. The pVAX1-HBs-MFC was transfected into HEK 293T cells and its expression was measured by ELISA and Western blotting. BALB/c mice were intramuscularly immunized with the pVAX1-HBs-MFC, and an ELISA approach was applied to determine the specific antibody titers and subtypes in the mouse serum. The cross-reactivity of the antibodies was also checked with two synthesized HCV hypervariable region 1 (HVR1) peptides. The IFN-γ production and cell proliferation of the mouse spleen cells were evaluated by ELISA and MTS (3-[4, 5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl] -2H-tetrazolium, inner salt) assays, respectively. The expression of pVAX1-HBs-MFC was detectable in the transfected HEK 293T cells. The serum antibody response was effectively elicited in BALB/c mice injected with pVAX1-HBs-MFC. The highest titer of antibody against HCV (MFC) was 1∶1280, and the ratio of IgG2a/IgG1 was 1.50±0.12 at the fifth week after first immunization. Moreover, the collected mouse serum antibody had the ability to cross-react with the two synthesized HCV HVR1 peptides. The stimulation index of the mouse splenocytes to MFC was 1.79 ±0.07, and the IFN-γ level was 287±6?pg/ml at week 21 after first immunization. The highest titer of the antibody in control BALB/c mice immunized with pVAX1-MFC was 1∶320, and the ratio of IgG2a/IgG1 was 1.33±0.11 at week 5 post-immunization. Furthermore, the stimulation index of the mouse splenocytes cells to MFC was 1.52±0.06, and the IFN-γ level was 225±9.3?pg/ml at week 21 post-immunization. The HBsAg gene can enhance the effects of an HCV multiple-epitope DNA construct on its humoral and cellular immune responses. This HBsAg enhanced HCV multiple-epitope DNA vector may be of potential use in the development of HCV vaccines. | YUPINGGONG JUNGAO PINGZHAO XIAOPINGYANG ZHONGTIANQI | 2005 | Journal of Microbiology and Immunology2005，3，1： | 1 |
| 3 | Synthesis of 2-Alkyl(aryl)-3-methylthio-6-methyl-6-arylpyrano-[4, 3-c] pyrazol-4(2H)-ones显示文摘The synthesis of 2-alkyl(aryl)-3-methylthiopyrano[4,3-c]pyrazol-4(2H)-ones via 5, 6- dihydro-2H-pyran-2, 4-dione-3-dithioacetals with (un)substituted hydrazines is described and the mechanism of the formation of title compounds is discussed. Their structures were confirmed by 1HNMR spectra and elemental analysis. | YuXinLI YouMingWANG XiaoPingYANG SuHuaWANG ZhengMingLI | 2004 | Chinese Chemical Letters2004，15，1： | 1 |
| 4 | Carbon Nanotube Array/Polymer Core/Shell Structured Composites with High Dielectric Permittivity, Low Dielectric Loss, and Large Energy Density显示文摘 | HaiyangLiu YangShen YuSong Ce‐WenNan YuanhuaLin XiaopingYang | 2011 | Adv. Mater2011，，： | 1 |
| 5 | Designed Boronate Ligands for Glucose-Selective Holographic Sen- sors 显示文摘 | XiaopingYang Mei-Ching Lee FelicitySartain | 2006 | Chem-A Eur J2006，12，： | 1 |
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