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| 1 | Integrated physiologic,proteomic,and metabolomic analyses of Malus halliana adaptation to saline–alkali stress显示文摘Saline–alkali stress is a severely adverse abiotic stress limiting plant growth.Malus halliana Koehne is an apple rootstock that is tolerant to saline–alkali stress.To understand the molecular mechanisms underlying the tolerance of M.halliana to saline–alkali stress,an integrated metabolomic and proteomic approach was used to analyze the plant pathways involved in the stress response of the plant and its regulatory mechanisms.A total of 179 differentially expressed proteins(DEPs)and 140 differentially expressed metabolites(DEMs)were identified.We found that two metabolite-related enzymes(PPD and PAO)were associated with senescence and involved in porphyrin and chlorophyll metabolism;six photosynthesis proteins(PSAH2,PSAK,PSBO2,PSBP1,and PSBQ2)were significantly upregulated,especially PSBO2,and could act as regulators of photosystem II(PSII)repair.Sucrose,acting as a signaling molecule,directly mediated the accumulation of D-phenylalanine,tryptophan,and alkaloid(vindoline and ecgonine)and the expression of proteins related to aspartate and glutamate(ASP3,ASN1,NIT4,and GLN1−1).These responses play a central role in maintaining osmotic balance and removing reactive oxygen species(ROS).In addition,sucrose signaling induced flavonoid biosynthesis by activating the expression of CYP75B1 to regulate the homeostasis of ROS and promoted auxin signaling by activating the expression of T31B5_170 to enhance the resistance of M.halliana to saline–alkali stress.The decrease in peroxidase superfamily protein(PER)and ALDH2C4 during lignin synthesis further triggered a plant saline–alkali response.Overall,this study provides an important starting point for improving saline–alkali tolerance in M.halliana via genetic engineering. | Xu-mei Jia Yan-fang Zhu Ya Hu Rui Zhang Li Cheng Zu-lei Zhu Tong Zhao Xiayi Zhang Yan-xiu Wang | 2019 | Horticulture Research2019,6,1: | 9 |
| 2 | Numerical simulations and comparative analysis of two- and three-dimensional circulating fluidized bed reactors for CO2 capture显示文摘Carbon dioxide(CO2),the main gas emitted from fossil burning,is the primary contributor to global warming.Circulating fluidized bed reactor(CFBR)is proved as an energy-efficient method for post-combustion CO2 capture.The numerical simulation by computational fluid dynamics(CFD)is believed as a promising tool to study CO2 adsorption process in CFBR.Although three-dimensional(3D)simulations were proved to have better predicting performance with the experimental results,two-dimensional(2D)simulations have been widely reported for qualitative and quantitative studies on gas-solid behavior in CFBR for its higher computational efficiency recently.However,the discrepancies between 2D and 3D simulations have rarely been evaluated by detailed study.Considering that the differences between the 2D and 3D simulations will vary substantially with the changes of independent operating conditions,it is beneficial to lower computational costs to clarify the effects of dimensionality on the numerical CO2 adsorption runs under various operating conditions.In this work,the comparative analysis for CO2 adsorption in 2D and 3D simulations was conducted to enlighten the effects of dimensionality on the hydrodynamics and reaction behaviors,in which the separation rate,species distribution and hydrodynamic characteristics were comparatively studied for both model frames.With both accuracy and computational costs considered,the viable suggestions were provided in selecting appropriate model frame for the studies on optimization of operating conditions,which directly affect the capture and energy efficiencies of cyclic CO2 capture process in CFBR. | Yefeng Zhou Yifan Han Yujian Lu Hongcun Bai Xiayi Hu Xincheng Zhang Fanghua Xie Xiao Luo Jingdai Wang Yongrong Yang | 2020 | Chinese Journal of Chemical Engineering2020,28,12: | 1 |
| 3 | Nonlinear characteristics analyses of particle motion for predicting flow regimes显示文摘Gas-solid flow regimes have a significant impact on particle transport and separation in a fluidized bed reactor.In this study,to determine flow regime transitions in gas-solid fluidized beds,an acoustic technique was used to detect and analyze the behavior of gas and solids.Algorithm complexity,fluctuation complexity,and Shannon entropy analyses of acoustic emission signals were performed to examine non linear system characteristics,and to determine the flow regime transiti on velocities uc,uk,and ufd-Moreover,using the standard deviation of pressure signals,pressure measurements and acoustic measurements were compared.The relative deviations(RDs)between the experimental and empirical values of uk were 8.8%,13.7%,8.8%,and 30.4%for the algorithm complexity,fluctuation complexity,Shannon entropy,and pressure signal standard deviation,respectively,while the respective RDs for Ufd were 15.7%,23.9%,15.7%,and 97.8%.The RDs between the experimental and empirical values of uc were all 6.4%.The experimental values obtained from acoustic signal measurements were therefore closer to the empirical values.In summary,the integration of non-intrusive acoustic measurements,complexity analysis,and Shannon entropy analysis is suitable for identifying flow regime transitions. | Panxing Kang Yujian Lu Lei Yang Libin Liu Xiayi(Eric)Hu Xiao Luo Hongbo Chen Yefeng Zhou Rui Zhang | 2020 | Particuology2020,18,6: | 1 |