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| 1 | Distinct mononuclear diploid cardiac subpopulation with minimal cell-cell communications persists in embryonic and adult mammalian heart显示文摘A small proportion of mononuclear diploid cardiomyocytes(MNDCMs),with regeneration potential,could persist in adult mammalian heart.However,the heterogeneity of MNDCMs and changes during development remains to be illuminated.To this end,12645 cardiac cells were generated from embryonic day 17.5 and postnatal days 2 and 8 mice by single-cell RNA sequencing.Three cardiac developmental paths were identified:two switching to cardiomyocytes(CM)maturation with close CM–fibroblast(FB)communications and one maintaining MNDCM status with least CM–FB communications.Proliferative MNDCMs having interactions with macrophages and non-proliferative MNDCMs(non-pMNDCMs)with minimal cell–cell communications were identified in the third path.The non-pMNDCMs possessed distinct properties:the lowest mitochondrial metabolisms,the highest glycolysis,and high expression of Myl4 and Tnni1.Single-nucleus RNA sequencing and immunohistochemical staining further proved that the Myl4^(+)Tnni1+MNDCMs persisted in embryonic and adult hearts.These MNDCMs were mapped to the heart by integrating the spatial and single-cell transcriptomic data.In conclusion,a novel non-pMNDCM subpopulation with minimal cell–cell communications was unveiled,highlighting the importance of microenvironment contribution to CM fate during maturation.These findings could improve the understanding of MNDCM heterogeneity and cardiac development,thus providing new clues for approaches to effective cardiac regeneration. | Miaomiao Zhu Huamin Liang Zhe Zhang Hao Jiang Jingwen Pu Xiaoyi Hang Qian Zhou Jiacheng Xiang Ximiao He | 2023 | Frontiers of Medicine2023，17，5： | 1 |
| 2 | Co_(3)O_(4) with ordered pore structure derived from wood vessels for efficient Hg^(0) oxidation显示文摘Catalytic oxidation of Hgo to Hg~O is an efficient way to remove Hg^(0) from coal-fired flue gas.The catalyst with ordered pore structure can lower mass transfer resistance resulting in higher Hg^(0) oxidation efficiency.Therefore,in the present work,wood vessels were used as sacrificial template to obtain Co_(3)O_(4) with ordered pore structure.SEM and BET results show that,when the mass concentrations of Co(NO_(3))_(2)·6H_(2)O was 20%,the obtained catalyst(Co_(3)O_(4) [20%Co(NO_(3))_(2)])possesses better pore structure and higher surface area.It will expose more available surface active sites and lower the mass transfer resistance.Furthermore,XPS results prove that Co_(3)O_(4) [20%Co(NO_(3))_(2)]has the highest ratio of chemisorbed oxygen which plays an important role in Hg^(0) oxidation process.These results lead to a better Hg^(0) oxidation efficiency of Co_(3)O_(4) [20%Co(NO_(3))_(2)],which is about 90%in the temperature range of 200 to 350℃,Furthermore,Co_(3)O_(4) [20%Co(NO_(3))_(2)]has a stable catalytic activity,and its Hg^(0) oxidation efficiency maintains above 90%at 250℃even after 90 h test,A probable reaction mechanism is deduced by the XPS results of the fresh,used and regenerated catalyst of Co_(3)O_(4) [20%Co(NO3)2].Chemisorbed oxygen can react with Hg^(0) forming HgO with the reduction of Co^(3+)to Co^(2)+.And lattice oxygen and gaseous oxygen can supplement the consumption of chemisorbed oxygen to oxidize Co^(2+)to Co^(3+). | Xiaopeng Zhang Cheng Gao Ziwei Wang Ximiao Wang Jie Cheng Xinxin Song Xiangkai Han Ning Zhang Junjiang Bao Gaohong He | 2022 | Chinese Journal of Chemical Engineering2022，，10： | 0 |
| 3 | The Most Redundant Sequences in Human CpG Island Library Are Derived from Mitochondrial Genome显示文摘An altered pattern of epigenetic modifications, such as DNA methylation and histone modification, is critical to many common human diseases, including cancer. Recently, mitochondrial DNA (mtDNA) was reported to be associated with tumorigenesis through epigenetic regulation of methylation patterns. One of the promising approaches to study DNA methylation and CpG islands (CGIs) is sequencing and analysis of clones derived from the physical library generated by methyl-CpG-binding domain proteins and restriction enzyme MseI. In this study, we observed that the most redundant sequences of 349 clones in a human CGI library were all generated from the human mitochondrial genome. Further analysis indicated that there was a 5,845-bp DNA transfer from mtDNA to chromosome 1, and all the clones should be the products of a 510-bp MseI fragment, which contained a putative CGI of 270 bp. The 510-bp fragment was annotated as part of cytochrome c oxidase subunit II (COXII), and phylogenetic analysis of homologous sequences containing COXII showed three DNA transfer events from mtDNA to nuclear genome, one of which underwent secondary transfer events between different chromosomes. These results may further our understanding of how the mtDNA regulates DNA methylation in the nucleus. | Ximiao He Shu Tao Jing Jin Songnian Hu Jun Yu | 2010 | Genomics, Proteomics & Bioinformatics2010，8，2： | 0 |
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