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| 1 | Syndecan-1 and E-cadherin expression in differentiated type of early gastric cancer显示文摘AIM: To elucidate the role and alterations of syndecan-1 and E-cadherin expression in different cellular phenotypes of differentiated-type gastric cancers (DGCs), METHODS: A total of 120 DGCs at an early stage, andtheir adjacent mucosa, were studied both by immunohistochemistry. Syndecan-1 and E-cadherin were assessed by immunohistochemical staining with anti-syndecan-1 and anti-E-cadherin antibodies, respectively. Based on immunohistochemistry, DGCs and their surrounding mucosa were divided into four types: gastric type (G-type),ordinary type (O-type), complete-intestinal type (CI-type),and null type (N-type).RESULTS: Syndecan-1 expression was significantly lower in G-type cancers (29.4%) than in O-type (79.6%) and CI-type cancers (90%) (P<0.05, respectively), but E-cadherin did not show this result. In addition, syndecan-1 expression was significantly reduced in DGCs comprised partly of poorly differentiated adenocarcinoma or signet-ring cell carcinoma, compared to DGCs demonstrating papillary and/or tubular adenocarcinoma (P<0.05). G-type intestinal metaplasia (IM) surrounding the tumors was observed in 23.8% of G-type, 4.9% of O-type, and 6.7% of CI-type cancers (P<0.05; G-type vs O-type). Reduction of syndecan-1 expression was significant in G-type IM (25%) compared to non-G-type IM (75%; P<0.05).CONCLUSION: Loss of syndecan-1 plays a role in the growth of G-type cancers of DGCs at an early stage, and the reduction of syndecan-1 expression in IM surrounding the tumors may influence the growth of G-type cancer. | Mei-FangHuang You-QingZhu Zhi-FenChen JunXiao XinHuang Yong-YanXiong Gui-FangYang | 2005 | World Journal of Gastroenterology2005，11，19： | 5 |
| 2 | Suppressive effects of 17β-estradiol on hepaUc fibrosis in CCl_4-induced rat model显示文摘AIM: To investigate the pathway via which 17β-estradio(β-Est) exerts suppressive effects on rat hepatic fibrosis.METHODS: In vivo study was done in CCI4-induced female hepatofibrotic rats. Fibrosis-suppressive effect of β-Est(20 μg/kg·d) was evaluated in intact and ovariectomized rat models. Six weeks after the treatment, all the rats were sacrificed and specimens of serum or liver tissue were collected for the studies. Serum liver enzymes,fibrosis markers and estradiol levels were determined by standard enzymatic methods, ELISA and RIA, respectively.Degrees of fibrosis and areas of hepatic stellate cells(HSCs) positive for alpha-smooth muscle actin (a-SMA) in the liver were determined by van Gieson (VG) stain and immunohistochemistry. In vitro studies, HSCs were isolated by a combination of pronase-collagenase perfusion and density gradient centrifugation. First-passage HSCs were randomly divided into 10 groups, and different concentrations of β-Est, 2-hydroxyestradiol (2OHE) or 2-methoxyestradiol(2MeOE) were separately added to the cell groups. After incubation for 72 h, the degree of cell proliferation, collagen production, ^-SMA or estrogen receptor (ER) expression was determined by MTT assay, ELISA and immunohistochemistry,respectively.RESULTS: β-Est treatment reduced aspartate aminotransfer-ase (AST), alanine aminotransferase (ALT), hyaluronic acid(HA) and type IV collagen (C IV) in sera, suppressed hepatic collagen content, decreased the areas of HSCs positive for α-SMA significantly in both intact and ovariectomized female hepatofibrotic rats. There was a negative correlation between the percentage of fibrotic area of liver tissue and the serum estradiol level; the calculated correlation coefficient was -0.57 (P<0.01). β-Est and its metabolites concentration-dependently (10^-9 mol/L-10^-7 mol/L) inhibited HSC proliferation and collagen synthesis. At the concentration of 10^-7 mol/L, they could inhibit α-SMA expression. The order of potency was 2MeOE>2OHE>β-Est.CONCLUSION: β-Est may suppress hepatic fibrosis probably via its biologically active metabolites. | Qing-HuaLiu Ding-GuoLi XinHuang Chun-HuaZong Qin-FangXu Han-MingLu | 2004 | World Journal of Gastroenterology2004，10，9： | 5 |
| 3 | Glucose Oxidase-graphene-chitosan modified e lectrode for direct electrochemistry and glucose sensing显示文摘 | Xinhuang Kang 等 | 2009 | Biosensors and Bioelectronics2009，25，： | 1 |
| 4 | A graphene based electrochemical sensor for sensitive de- tection of paracetamol显示文摘 | KANG Xinhuang WANG Jun WU Hong | 2010 | Talanta2010，81，： | 1 |
| 5 | A novel glucose biosensor based on immobilization of glucose oxidase in chitosan on a glassy carbon electrode modified with gold-platinum alloy nanoparticles/muhiwall carbon nanotubes 显示文摘 | KANG Xinhuang MAI Zhibin ZOU Xiaoyong | 2007 | Anal Biochem2007，369，： | 1 |
| 6 | A novel glucose biosensor based on immobilization of glucose oxidase in chitosan on a glassy carbon electrode modified with gold-platinum alloy nanoparticles/ multiwall carbon nanotubes显示文摘 | Xinhuang Kang Zhibin Mai Xiaoyong Zou | 2007 | Analytical Biochemistry2007，，369： | 1 |
| 7 | Glucose Biosensors Based on Platinum Nanoparticles-Deposited Carbon Nanotubes in Sol-Gel Chitosan/Silica Hybrid显示文摘 | Kang Xinhuang Mai Zhibin Zou Xiaoyong Cai Peixiang and Mo Jinyuan | 2008 | Talanta2008，74，4： | 1 |
| 8 | Glucose biosensors based on platinum nanoparticles- deposited carbon nanotubes in sol-gel ehitosan/silica hybrid显示文摘 | Xinhuang Kang Zhibin Mai Xiaoyong Zou | 2008 | Talanta2008，，74： | 1 |
| 9 | A three - dimensional simulation of a spray dryer fitted with a ro- tary atomizer显示文摘 | LI Xinhuang M L Passos Kurichi Kumar | 2005 | Drying Technology2005，23，： | 1 |
| 10 | 显示文摘 | Kang Xinhuang Wang Jun Wu Hong | 2009 | Biosens Bioel- ectron2009，25，4： | 1 |
| 11 | A sensitive nonenzymatic glucose sensor in alkaline media with a copper nanocluster/multiwall carbon nanotube-modified glassy carbon electrode显示文摘 | Xinhuang Kang Zhibin Mai Xiaoyong Zou Peixiang Cai Jinyuan Mo | 2007 | Analytical Biochemistry2007，，1： | 1 |
| 12 | Numerical study of two-stage hori- zontal spray dryers using computational fluid dynamics 显示文摘 | Li Xinhuang Mujumdar A S | 2006 | Drying Technology2006，24，6： | 1 |
| 13 | Glucose oxidasegraphene-chitosan modifiedelectrode for direct electrochemistry and glucosesensing显示文摘 | KANG Xinhuang WANG Jun WU Hong | 2009 | Biosensorsors and Bioelectronics2009，25，4： | 1 |
| 14 | Glucose biosensor based onimmobilization of glucoseoxidase in platinum nanoparticles/graphene/chitosan nanocomposite film显示文摘 | WU Hong WANG Jun KANG Xinhuang | 2009 | Talanta2009，80，1： | 1 |
| 15 | The reduce of ductility in steel billet containing Nb and the precipitation of Nb ( C, N ) between 700C and 1000C 显示文摘 | Wang Xinhuang Changbo Li jingjie | 1997 | Acta metallurgica sinica1997，33，5： | 1 |
| 16 | A novel glucose biosensor based on immobilization of glucose oxidase in chitosan on a glassy carbon electrode modified with gold-platinum alloy nanoparticles/multiwall carbon nanotubes显示文摘 | Kang Xinhuang Mai Zhibin Zou Xiaoyong | 2007 | Analytical Biochemistry2007，369，1： | 1 |
| 17 | Glucose oxi- dase-graphene-chitosan modified electrode for direct electro- chemistry and glucose sensing 显示文摘 | Kang Xinhuang Wang Jun Wu Hong | 2009 | Biosens Bioelectr2009，25，： | 1 |
| 18 | Glucose bio- sensor based on immobilization of glucose oxidase in plati- num nanoparticles/graphene/chitosan nanoeomposite film 显示文摘 | Wu Hong Wang Jun Kang Xinhuang | 2009 | Talanta2009，80，： | 1 |
| 19 | A graphene- based electrochemical sensor for sensitive detection of para- cetamol 显示文摘 | Kang Xinhuang Wang Jun Wu Hong | 2010 | Talanta2010，81，： | 1 |
| 20 | Flood hazard in Hunan province of China:an economic loss analysis显示文摘 | Xinhuang HongzhuanTan Jiazhou | 2008 | Natural Hazards2008，，47： | 1 |
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