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您的检索式：作者名="Xintong Sui"
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| 1 | A detached petal disc assay and virus-induced gene silencing facilitate the study of Botrytis cinerea resistance in rose flowers显示文摘Fresh-cut roses(Rosa hybrida)are one of the most important ornamental crops worldwide,with annual trade in the billions of dollars.Gray mold disease caused by the pathogen Botrytis cinerea is the most serious fungal threat to cut roses,causing extensive postharvest losses.In this study,we optimized a detached petal disc assay(DPDA)for artificial B.cinerea inoculation and quantification of disease symptoms in rose petals.Furthermore,as the identification of rose genes involved in B.cinerea resistance could provide useful genetic and genomic resources,we devised a virusinduced gene silencing(VIGS)procedure for the functional analysis of B.cinerea resistance genes in rose petals.We used RhPR10.1 as a reporter of silencing efficiency and found that the rose cultivar‘Samantha’showed the greatest decrease in RhPR10.1 expression among the cultivars tested.To determine whether jasmonic acid and ethylene are required for B.cinerea resistance in rose petals,we used VIGS to silence the expression of RhLOX5 and RhEIN3(encoding a jasmonic acid biosynthesis pathway protein and an ethylene regulatory protein,respectively)and found that petal susceptibility to B.cinerea was affected.Finally,a VIGS screen of B.cinerea-induced rose transcription factors demonstrated the potential benefits of this method for the high-throughput identification of gene function in B.cinerea resistance.Collectively,our data show that the combination of the DPDA and VIGS is a reliable and highthroughput method for studying B.cinerea resistance in rose. | Xiaoqian Cao Huijun Yan Xintong Liu Dandan Li Mengjie Sui Jie Wu Hongqiang Yu Zhao Zhang | 2019 | Horticulture Research2019，6，1： | 5 |
| 2 | Exosomal lncRNA-p21 derived from mesenchymal stem cells protects epithelial cells during LPS-induced acute lung injury by sponging miR-181显示文摘Long noncoding RNAs(lncRNAs)act as essential regulators of various diseases.However,the functions of lncRNAs in sepsis-induced acute lung injury(SALI)remain unclear.Here,we found that lipopolysaccharide could upregulate lncRNA-p21 expression in mesenchymal stem cells(MSCs)in a time-and dose-dependent manner and that lncRNA-p21 was packaged into exosomes.Furthermore,we demonstrated that treatment with exosomal lncRNA-p21 could increase the expression of sirtuin 1(SIRT1)to protect MLE-12 cells from apoptosis during sepsis.Moreover,we identified SIRT1 as a direct target of miR-181 and found that the level of SIRT1 was negatively correlated with the level of miR-181.The luciferase reporter assay also confirmed the negative correlation between the levels of miR-181 and lncRNA-p21.Our results showed that the lncRNA-p21-induced downregulation of miR-181 might suppress epithelial cell apoptosis and alleviate lung tissue injury by upregulating SIRT1 expression,suggesting the potential therapeutic effects of lncRNA-p21 on SALI.In conclusion,we found that the novel lncRNA-p21/miR-181/SIRT1 pathway may play an important role in the progression of SALI,and MSC-derived exosomes may be a new therapeutic strategy for this disease. | Xintong Sui Wei Liu Zhi Liu | 2021 | Acta Biochimica et Biophysica Sinica2021，53，6： | 1 |
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