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您的检索式：作者名="Xionglun Liu"
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| 1 | Engineering broad-spectrum disease-resistant rice by editing multiple susceptibility genes显示文摘Rice blast and bacterial blight are important diseases of rice(Oryza sativa)caused by the fungus Magnaporthe oryzae and the bacterium Xanthomonas oryzae pv.oryzae(Xoo),respectively.Breeding rice varieties for broadspectrum resistance is considered the most effective and sustainable approach to controlling both diseases.Although dominant resistance genes have been extensively used in rice breeding and production,generating diseaseresistant varieties by altering susceptibility(S)genes that facilitate pathogen compatibility remains unexplored.Here,using CRISPR/Cas9 technology,we generated loss-of-function mutants of the S genes Pi21 and Bsr-d1 and showed that they had increased resistance to M.oryzae.We also generated a knockout mutant of the S gene Xa5 that showed increased resistance to Xoo.Remarkably,a triple mutant of all three S genes had significantly enhanced resistance to both M.oryzae and Xoo.Moreover,the triple mutant was comparable to the wild type in regard to key agronomic traits,including plant height,effective panicle number per plant,grain number per panicle,seed setting rate,and thousand-grain weight.These results demonstrate that the simultaneous editing of multiple S genes is a powerful strategy for generating new rice varieties with broadspectrum resistance. | Hui Tao Xuetao Shi Feng He Dan Wang Ning Xiao Hong Fang Ruyi Wang Fan Zhang Min Wang Aihong Li Xionglun Liu Guo-Liang Wang Yuese Ning | 2021 | Journal of Integrative Plant Biology2021，63，9： | 4 |
| 2 | Function of hydroxycinnamoyl transferases for the biosynthesis of phenolamides in rice resistance to Magnaporthe oryzae显示文摘Phenolamide(PA)metabolites play important roles in the interaction between plants and pathogens.The putrescine hydroxycinnamoyl transferase genes Os PHT3 and Os PHT4 positively regulate rice cell death and resistance to Magnaporthe oryzae.The b ZIP transcription factor APIP5,a negative regulator of cell death and rice immunity,directly binds to the Os PHT4 promoter to regulate putrescine-derived PAs.Whether other hydroxycinnamoyl transferase(HT)genes also participate in APIP5-mediated immunity remains unclear.Surprisingly,we find that genes encoding agmatine hydroxycinnamoyl transferases Os AHT1 and Os AHT2,tryptamine hydroxycinnamoyl transferases Os TBT1 and Os TBT2,and tyramine hydroxycinnamoyl transferases Os THT1 and Os THT2,responsible for the biosynthesis of polyamine-derived PAs are all up-regulated in APIP5-RNAi transgenic plants compared with segregated wild-type rice.Furthermore,both Os AHT1/2 and Os TBT1/2 are induced during M.oryzae infection,showing expression patterns similar to those previously reported for Os THT1/2 and Os PHT3/4.Transgenic plants overexpressing either Os AHT2-GFP or Os TBT1-GFP show enhanced resistance against M.oryzae and accumulated more PA metabolites and lignin compared with wild-type plants.Interestingly,as demonstrated for Os PHT4,APIP5 directly binds to the promoters of Os AHT1/2,Os TBT1/2,and Os THT1/2,repressing their transcription.Together,these results indicate that the HT genes are common targets of APIP5 and that PAs play critical roles in rice immunity. | Hong Fang Fan Zhang Chongyang Zhang Dan Wang Shuangqian Shen Feng He Hui Tao Ruyi Wang Min Wang Debao Wang Xionglun Liu Jie Luo Guo-Liang Wang Yuese Ning | 2022 | Journal of Genetics and Genomics2022，49，8： | 1 |
| 3 | Molecular mapping of the blast resistance gene Pi49 in the durably resistant rice cultivar Mowanggu显示文摘 | Pingyong Sun Jinling Liu Yue Wang Nan Jiang Suhua Wang Yangshuo Dai Jia Gao Zhiqiang Li Sujun Pan Dan Wang Wei Li Xionglun Liu Yinghui Xiao Erming Liu Guo-Liang Wang Liangying Dai | 2013 | Euphytica2013，，1： | 1 |
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