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| 1 | Nitrate regulates rhizobial and mycorrhizal symbiosis in common bean(Phaseolus vulgaris L.)显示文摘Nitrogen‐limited conditions are considered to be a prerequisite for legume‐rhizobial symbiosis, but the effects of nitrate‐rich conditions on symbiotic status remain poorly understood. We addressed this issue by examining rhizobial(Rhizobim tropici) and arbusclarmycorrhizal(Glomus intraradices) symbiosis in Phaseolus vulgaris L. cv. Negro Jamapa under nitrate pre‐incubation and continuous nitrate conditions. Our results indicate that nitrate pre‐incubation, independent of the concentration, did not affect nodule development. However, the continuous supply of nitrate at high concentrations impaired nodule maturation and nodule numbers. Low nitrate conditions, in addition to positively regulating nodule number, biomass, and nitrogenase activity, also extended the span of nitrogen‐fixing activity. By contrast, for arbuscular mycorrhizae, continuous 10 and 50mmol/L nitrate increased the percent root lengthcolonization, concomitantly reduced arbuscule size, and enhanced ammonia transport without affecting phosphate transport. Therefore, in this manuscript, we have proposed the importance of nitrate as a positive regulator in promoting both rhizobial and mycorrhizal symbiosis in the common bean. | Kalpana Nanjareddy Lourdes Blanco Manoj‐Kumar Arthikala Xochitl Alvarado Affantrange Federico Sánchez Miguel Lara | 2014 | Journal of Integrative Plant Biology2014，56，3： | 3 |
| 2 | The Treatment-Naive Microbiome in New-Onset Crohn’s Disease显示文摘 | Dirk Gevers Subra Kugathasan Lee A. Denson Yoshiki Vázquez-Baeza Will Van Treuren Boyu Ren Emma Schwager Dan Knights Se Jin Song Moran Yassour Xochitl C. Morgan Aleksandar D. Kostic Chengwei Luo Antonio González Daniel McDonald Yael Haberman Thomas Walter | 2014 | Cell Host & Microbe2014，，： | 3 |
| 3 | Isolation, screening and identifi cation of Bacillus spp. as direct-fed microbial candidates for aflatoxin B_1 biodegradation显示文摘Objective: To evaluate the ability of Bacillus spp. as direct-fed microbials(DFM) to biodegrade al atoxin B1(AFB1) by using an in vitro digestive model simulating in vivo conditions.Methods: Sixty-nine Bacillus isolates were obtained from intestines, and soil samples were screened by using a selective media method against 0.25 and 1.00 μg/m L of AFB1 in modii ed Czapek-Dox medium. Plates were incubated at 37 °C and observed every two days for two weeks. Physiological properties of the three Bacillus spp. candidates were characterized biochemically and by 16 S r RNA sequence analyzes for identii cation. Tolerance to acidic p H, osmotic concentrations of Na Cl, bile salts were tested, and antimicrobial sensitivity proi les were also determined. Bacillus candidates were individually sporulated by using a solid fermentation method and combined. Spores were incorporated into 1 of 3 experimental feed groups: 1) Negative control group, with unmedicated starter broiler feed without AFB1; 2) Positive control group, with negative control feed contaminated with 0.01% AFB1; 3) DFM treated group, with positive control feed supplemented with 109 spores/g. After digestion time(3:15 h), supernatants and digesta were collected for high-performance liquid chromatography l uorescence detection analysis by triplicate.Results: Three out of those sixty-nine DFM candidates showed ability to biodegrade AFB1 in vitro based on growth as well as reduction of l uorescence and area of clearance around each colony in modii ed Czapek-Dox medium which was clearly visible under day light after 48 h of evaluation. Analysis of 16S-DNA identii ed the strains as Bacillus amyloliquefaciens, Bacillus megaterium and Bacillus subtilis. The three Bacillus strains were tolerant to acidic conditions(p H 2.0), tolerant to a high osmotic pressure(Na Cl at 6.5%), and were able to tolerate 0.037% bile salts after 24 h of incubation. No signii cant dif erences(P > 0.05) were observed in the concentrations of AFB1 in neither the supernatants nor digesta samples evaluated by highperformance liquid chromatography with l uorescence detection between positive control or DFM treated groups. Conclusions: In vitro digestion time was not enough to confirm biodegradation of AFB1. Further studies to evaluate the possible biodegradation ef ects of the BacillusDFM when continuously administered in experimentally contaminated feed with AFB1, are in progress. | Rosario Galarza-Seeber Juan David Latorre Xochitl Hernandez-Velasco Amanda Drake Wolfenden Lisa Renee Bielke Anita Menconi Billy Marshall Hargis Guillermo Tellez | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，9： | 3 |
| 4 | SRB-biofilm influence in active corrosion sites formed at the steel-electrolyte interface when exposed to artificial seawater conditions显示文摘 | Homero Castaneda Xochitl D. Benetton | 2007 | Corrosion Science2007，，4： | 2 |
| 5 | Evaluation of curcumin and copper acetate against Salmonella Typhimurium infection,intestinal permeability,and cecal microbiota composition in broiler chickens显示文摘Background:Interest in the use of natural feed additives as an alternative to antimicrobials in the poultry industry has increased in recent years because of the risk of bacterial resistance.One of the most studied groups are polyphenolic compounds,given their advantages over other types of additives and their easy potentiation of effects when complexes are formed with metal ions.Therefore,the objective of the present study was to evaluate the impact of dietary supplementation of copper acetate(CA),curcumin(CR),and their combination(CA-CR)against Salmonella Typhimurium colonization,intestinal permeability,and cecal microbiota composition in broiler chickens through a laboratory Salmonella infection model.S.Typhimurium recovery was determined on day 10 post-challenge by isolating Salmonella in homogenates of the right cecal tonsil(12 chickens per group)on Xylose Lysine Tergitol-4(XLT-4)with novobiocin and nalidixic acid.Intestinal integrity was indirectly determined by the fluorometric measurement of fluorescein isothiocyanate dextran(FITC-d)in serum samples from blood obtained on d 10 post-S.Typhimurium challenge.Finally,microbiota analysis was performed using the content of the left caecal tonsil of 5 chickens per group by sequencing V4 region of 16S rRNA gene.Results:The results showed that in two independent studies,all experimental treatments were able to significantly reduce the S.Typhimurium colonization in cecal tonsils(CT,P<0.0001)compared to the positive control(PC)group.However,only CA-CR was the most effective treatment in reducing S.Typhimurium counts in both independent studies.Furthermore,the serum fluorescein isothiocyanate dextran(FITC-d)concentration in chickens treated with CR was significantly lower when compared to PC(P=0.0084),which is related to a decrease in intestinal permeability and therefore intestinal integrity.The effect of dietary treatments in reducing Salmonella was further supported by the analysis of 16S rRNA gene sequences using Linear discriminant analysis effect size(LEfSe)since Salmonella was significantly enriched in PC group(LDA score>2.0 and P<0.05)compared to other groups.In addition,Coprobacillus,Eubacterium,and Clostridium were significantly higher in the PC group compared to other treatment groups.On the contrary,Fecalibacterium and Enterococcus in CR,unknown genus of Erysipelotrichaceae at CA-CR,and unknown genus of Lachnospiraceae at CA were significantly more abundant respectively.Conclusions:CR treatment was the most effective treatment to reduce S.Typhimurium intestinal colonization and maintain better intestinal homeostasis which might be achieved through modulation of cecal microbiota. | Anaisa ALeyva-Diaz Daniel Hernandez-Patlan Bruno Solis-Cruz Bishnu Adhikari Young Min Kwon Juan D.Latorre Xochitl Hernandez-Velasco Benjamin Fuente-Martinez Billy M.Hargis Raquel Lopez-Arellano Guillermo Tellez-Isaias | 2021 | Journal of Animal Science and Biotechnology2021，12，3： | 2 |
| 6 | Biological recycling of used baby diapers in a small-scale composting system显示文摘 | Rosa M. Espinosa-Valdemar Perla X. Sotelo-Navarro Xochitl Quecholac-Pi?a Mariel A. García-Rivera Margarita Beltrán-Villavicencio Sara Ojeda-Benítez Alethia Vázquez-Morillas | 2014 | Resources, Conservation & Recycling2014，，： | 1 |
| 7 | Thermogravimetric studies on the effect of support in sorbent-materials applied to flue gas desulfurization 显示文摘 | ROBERTO F ARTURO R CINTHYA P YULIANA M XOCHITL M LAURA G | 2008 | Fuel2008，87，： | 1 |
| 8 | SRB-biofilm influence in active corrosion sites formed at the steel electrolyte interface when exposed to artificial seawater conditions显示文摘 | Castaneda Homero Benetton Xochitl D | 2008 | Corrosion Science2008，50，4： | 1 |
| 9 | Evaluation of vapor recovery systems efficiency and personal exposure in service stations in Mexico City显示文摘 | Xochitl Cruz-N俨 eza José M.Hernández-Solísa Luis G. Ruiz-Suárez | | 0，，： | 1 |
| 10 | SRB-biofilm influence in active corrosion sites formed at the steel-electrolyte interface when exposed to artificial seawater conditions显示文摘 | Homero Castaneda Xochitl D. Benetton | 2007 | Corrosion Science2007，，4： | 1 |
| 11 | Internal resistance of microfluidic microbial fuel cell:Challenges and potential opportunities显示文摘 | Ahmed El Mekay Hanaa M Hegab Xochitl Dominguez-Benetton | 2013 | Bioresource Technology2013，142，： | 1 |
| 12 | Distribution and Expression Pattern of Claudins 6, 7, and 9 in Diffuse- and Intestinal-Type Gastric Adenocarcinomas显示文摘 | Erika Rendón-Huerta Fortoul Teresa Gorráez María Teresa Garcia-Samper Xochitl Alvarez-Fernández Georgina Zavala-Zendejas Veronica Luis Felipe Monta?o | 2010 | Journal of Gastrointestinal Cancer2010，，1： | 1 |
| 13 | Evaluation of vapor recovery systems efficiency and personal exposure in service stations in Mexico City显示文摘 | Cruz-Nunez Xochitl Hemandez-Solis Jose M Ruiz-Suarez Luis G | 2003 | The Science of the Total Environment2003，309，： | 1 |
| 14 | Comparison of total immunoglobulin A levels in different samples in Leghorn and broiler chickens显示文摘Objective: To standardize an ELISA protocol to quantify total immunoglobulin A(IgA)from different biological samples.Methods: Two independent experiments were conducted. In Experiment 1, total IgA levels were quantified from the lachrymal fluid, tracheal swab, and cloacal swab at various time points from Days 30 to 89 in white Leghorn chickens. Experiment 2 was conducted to evaluate the effect of 50 or 500 ppb of aflatoxin B1(AFB1) on total IgA quantified in samples from the lachrymal fluid, tracheal swab, gut content and cloacal swab in broiler chickens at 21 days of age.Results: Lachrymal fluid contained the highest level of IgA; however, the sampling procedure was time consuming and stressful to the bird, and the sample volume depends largely on the size of the chicken. Cloacal swabs also contained a high concentration of IgA; this sampling procedure was faster than lachrymal fluid sampling and was not affected by the age of the bird. Tracheal sampling was more difficult than cloacal sampling; the age of the bird limited the sampling, and the IgA concentration was the lowest detected at all sampling ages. 500 ppb of AFB1 significantly reduced total IgA concentration in the gut content compared with control or 50 ppb of AFB1 treated groups.Interestingly, a significant reduction in total IgA was also observed in those chickens that received 50 ppb of AFB1 in gut rinse when compared with cloacal swabs.Conclusions: The results of this study suggest that cloacal swab is an easy and reliable way to evaluate mucosal IgA concentration in both Leghorn and broiler chickens. | Rubén Merino-Guzmán Juan David Latorre Ramiro Delgado Xochitl Hernandez-Velasco Amanda Desha Wolfenden Kyle Dean Teague Lucas Elzie Graham Brittany Danielle Mahaffey Mikayla Francis Ann Baxter Billy Marshall Hargis Guillermo Tellez | 2017 | Asian Pacific Journal of Tropical Biomedicine2017，7，2： | 0 |
| 15 | Effect of Chia Seed on Physicochemical and Sensory Characteristics of Common Carp Restructured as Functional Food显示文摘 | Angel Santillan-Alvarez Octavio Dublain-Garcia Leticia Xochitl Lopez-Martinez Baciliza Quintero-Salazar Leobardo Manuel Gomez-Olivan Daniel Diaz-Bandera Maria Dolores Hemandez-Navarro | 2017 | Journal of Food Science and Engineering2017，7，3： | 0 |
| 16 | Assessment of the Connection of Antisocial-Criminal Behavior, Corruption and Depression in College Students显示文摘 | Iris Xochitl Galicia Moyeda Alejandra Sanchez Velasco Francisco Javier Robles Ojeda | 2017 | Psychology Research2017，7，3： | 0 |
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