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| 1 | The Matrix Stiffness and Physical Confinement of Hydrogel Microchannel Jointly Induce the Mesenchymal-Amoeboid Transition for Cancer Cell Migration显示文摘The migration mode transition of cancer cell enhances its invasive capability and the drug resistance,where physical confinement of cell microenvironment has been revealed to induce the mesenchymal-amoeboid transition(MAT).However,most existing studies are performed in PDMS microchannels,of which the stiffness is much higher than that of most mammalian tissues.Therefore,the amoeboid migration transition observed in these studies is actually induced by the synergistic effect of matrix stiffness and confinement.Since the stiffness of cell microenvironment has been reported to influence the cell migration in 2D substrate,the decoupling of stiffness and confinement effects is thus in need for elucidating the underlying mechanism of MAT.However,it is technically challenging to construct microchannels with physiologically relevant stiffness and channel size,where existing microchannel platforms with physiological relevance stiffness are all with>10μm channel width.Such size is too wide to mimic the physical confinement that migrating cancer cells confront in vivo,and also larger than the width of PDMS channel,in which the MAT of cancer cell was observed.Therefore,an in vitro cell migration platform,which could mimic both stiffness and confinement of the native physical microenvironment during cancer metastasis,could profoundly contribute to researches on cancer cell migration and cellular mechanotransduction.In this paper,we overcome the limitations of engineering soft materials in microscale by combining the collagen-alginate hydrogel with photolithography.This enables us to improve the accuracy of molded microchannel,and thus successfully construct a 3D microchannel platform,which matches the stiffness and width ranges of native environmental confinement that migrating cancer cells confront in vivo.The stiffness(0.3~20 kPa),confinement(channel width:3.5~14μm)and the adhesion ligand density of the microchannel can be tuned independently.Interestingly,using this platform,we observed that the migration speed of cancer cell is influenced by the synergistic effect of channel stiffness and width,and the increasing stiffness reverses the effect of channel width on the migration speed of cancer cells.In addition,MAT has a strong correlation with the channel stiffness.These findings make us reconsider the widely accepted hypothesis:physical confinement can induce MAT.Actually,this transition can only occur in stiff confined microenvironment not in soft one.For soft microchannels,the compliance of the channel walls could cause little cell/nucleus deformation,and the MAT could not be induced.To further investigate the mechanism of MAT,we developed a computational model to simulate the effect of nucleus deformation on MAT.With the model,we found that deforming the cell nuclear by decreasing the nucleus stiffness will reduce the cellmigration speed.This implies that nuclear stiffness plays an important role in the regulation of cancer migration speed and thus MAT in microchannels.The effect of channel stiffness on MAT and migration speed as observed in our experiment could partially explain previous findings reported in the literature,where the increasing matrix stiffness of tumor microenvironment promotes cancer metastasis.Our observations thus highlight the critical role of cell nuclear deformation not only in MAT,but also in regulating cellular mechanotransduction and cell-ECM interactions.This developed platform is capable of mimicking the native physical microenvironment during metastasis,providing a powerful tool for high-throughput screening applications and investigating the interaction between cancer migration and biophysical microenvironment. | Meng Wang Bo Cheng Yaowei Yang Han Liu Guoyou Huang Fei Li Feng Xu | 2019 | 医用生物力学2019，34，A01： | 2 |
| 2 | Integrated method for measuring distance and time difference between small satellites显示文摘The advancement of small satellites is promoting the development of distributed satellite systems,and for the latter,it is essential to coordinate the spatial and temporal relations between mutually visible satellites.By now,dual one-way ranging(DOWR)and two-way time transfer(TWTT)are generally integrated in the same software and hardware system to meet the limitations of small satellites in terms of size,weight and power(SWaP)consumption.However,studies show that pseudo-noise regenerative ranging(PNRR)performs better than DOWR if some advanced implementation technologies are employed.Besides,PNRR has no requirement on time synchronization.To apply PNRR to small satellites,and meanwhile,meet the demand for time difference measurement,we propose the round-way time difference measurement,which can be combined with PNRR to form a new integrated system without exceeding the limits of SWaP.The new integrated system can provide distributed small satellite systems with on-orbit high-accuracy and high-precision distance measurement and time difference measurement in real time.Experimental results show that the precision of ranging is about 1.94 cm,and that of time difference measurement is about 78.4 ps,at the signal to noise ratio of 80 dBHz. | ZHU Yaowei XU Zhaobin JIN Xiaojun GUO Xiaoxu JIN Zhonghe | 2021 | Journal of Systems Engineering and Electronics2021，32，3： | 1 |
| 3 | Bearings-only location using nonlinear least squares, national aerospace and electronics conference显示文摘 | Xu Yaowei Zhou Yiyu | 1997 | Proceedings of the IEEE1997，2，7： | 1 |
| 4 | Bearings-only location using nonlinear least squares, national aerospace and electronics conference显示文摘 | Mo Longbin Xu Yaowei Zhou Yiyu | 1997 | Proceedings of the IEEE1997，2，7： | 1 |
| 5 | Efficiency analysis of sorption-enhanced method in steam methane reforming process显示文摘The sorption-enhanced method can change the thermodynamic equilibrium by absorbing CO_(2).However,it also brings about the problems of high regeneration temperature of adsorbent and large regeneration energy consumption.In order to study the impact of enhanced adsorption methods on the overall energy cost of the system in the hydrogen production process,this paper analyzes and compares steam methane reforming and reactive adsorption-enhanced steam methane reforming with the energy consumption of hydrogen production products as the evaluation index.The results showed that the energy consumption per unit hydrogen production decreased from 276.21 MJ/kmol to 131.51 MJ/kmol,and the decomposition rate of H2O increased by more than 20%after the addition of adsorption enhancement method.It is proved that the advantage of sorption enhanced method on pre-separation of CO_(2)in the product makes up for the disadvantage of energy consumption of adsorbent regeneration.In addition,the ability of the process to obtain H element is improved by the high decomposition rate of H2O,which realizes a more rational distribution of the element. | Yaowei Hu Lu Liu Kai Xu Yuncai Song Jieying Jing Huiyan Zhang Jie Feng | 2023 | Carbon Resources Conversion2023，6，2： | 0 |
| 6 | A modular cable-driven humanoid arm with anti-parallelogram mechanisms and Bowden cables显示文摘This paper proposes a novel modular cable-driven humanoid arm with anti-parallelogram mechanisms(APMs)and Bowden cables.The lightweight arm realizes the advantage of joint independence and the rational layout of the driving units on the base.First,this paper analyzes the kinematic performance of the APM and uses the rolling motion between two ellipses to approximate a pure-circular-rolling motion.Then,a novel type of one-degree-of-freedom(1-DOF)elbow joint is proposed based on this principle,which is also applied to design the 3-DOF wrist and shoulder joints.Next,Bowden cables are used to connect the joints and their driving units to obtain a modular cable-driven arm with excellent joint independence.After that,both the forward and inverse kinematics of the entire arm are analyzed.Last,a humanoid arm prototype was developed,and the assembly velocity,joint motion performance,joint stiffness,load carrying,typical humanoid arm movements,and repeatability were tested to verify the arm performance. | Bin WANG Tao ZHANG Jiazhen CHEN Wang XU Hongyu WEI Yaowei SONG Yisheng GUAN | 2023 | Frontiers of Mechanical Engineering2023，18，1： | 0 |
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