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| 1 | Cloning and expression of AtPLC6, a gene encoding a phosphatidylinositol-specific phospholipase C in Arabidopsis thaliana显示文摘A full-length cDNA clone corresponding to a putative phosphatidylinositol-specific phospholipase C (PI- PLC) was isolated from Arabidopsis thaliana by screening a cDNA library and using RT-PCR strategy. The cDNA, des-ignated AtPLC6, encodes a putative polypeptide of 578 amino acid residues with a calculated molecular mass of 66251.84 D and a pI of 7.24. The sequence analysis indicates that the polypeptide contains X, Y, EF-hand and C2 domains. The overall structure of putative AtPLC6 protein, like other plant PI-PLCs, is most similar to that of mammalian PLCd. The recombinant AtPLC6 protein expressed in E. coli was able to hydrolyze phosphatidylinositol 4,5-biophosphate (PIP2) to generate inositol 1,4,5-trisphate (IP3) and 1,2-diacylglycerol (DAG). The protein hydrolyzes PIP2 in a Ca2+-dependent manner and the optimum concentration of Ca2+ is 10 mmol/L. These results suggested that AtPLC6 gene encodes a genuine PI-PLC. Northern blot analysis showed that the AtPLC6 gene is expressed at low level in all examined tissues, such as roots, stems, leaves, flowers, siliques and seedlings under normal growth conditions. The gene is strongly induced under low temperature and weakly induced under various stresses, such as ABA, high-salt stress and heat. These results sug-gested that AtPLC6 might be involved in the signal-trans- duction pathways of cold responses of the plants. | XUXiaojing CAOZhixiang LIUGuoqin MadanK.Bhattacharrya~ RENDongtao | 2004 | Chinese Science Bulletin2004，49，6： | 5 |
| 2 | Improvement of Strength in 2024 Al Alloy by Enhanced Solution Treatment显示文摘In present paper, the effect of enhanced solution on mechanical properties and aging behavior in a commercially available 2024A1 alloy were investigated using tension test and scanning electron microscopy. Differential scanning calorimeter (DSC) was used to measure the incipient melting behavior and then determine the technology parameter of enhanced solution heat treatment for high strength. The results show that an appropriately enhanced solution treatment can make final solution temperature higher than that of the conventional solution temperature and improves second phase solution without the formation of overheated microstructure. As the results, both the strength especially the yield strength and the resistance to over aging can be improved considerably without a large deterioration to tensile ductility. | XuXiaojing ZhangJie ChenKangming WangHongyu KimSeocksam | 2004 | 材料热处理学报2004，25，5： | 4 |
| 3 | Prokaryotic expression and characterization of a pea actin isoform (PEAc1) fused to GFP显示文摘Actins widely exist in eukaryotic cells and play important roles in many living activities. As there are many kinds of actin isoforms in plant cells,it is difficult to purifyeach actin isoform in sufficient quantities for analysing itsphysicochemical properties. In the present study, apea(pisum Sativum L.)actin isoform (PEAc1)fused to His-tag at its amino terminus and GFP(green fluorescent protein)atits Carboxyl terminus were expressed in E. coli in inclusionbodies. The fusion protein (PEAc1-GFP)was highly purifiedwith the yield of above 2 mg/L culture by dissolving inclu-sions in 8 mol/L urea,renaturing by dialysis in a gradient of urea,and affinity binding to Ni-resin. The purified mono-meric PEAc1-GFP could efficiently bind on DNase I andinhibit the latter抯 enzyme activity. PEAc1-GFP could po-lymerise into green fluorescent filamentous structures(F-PEAc1-GFP),which could be labelled byTRITC-phalloidin,a specific agent for observing microfila-ments. The PEAc1-GFP polymerlzation curve was identicalwith that of chicken skeletal muscle actin. The critical con-centration for PEAc1-Gfp to polymerise into filaments is 0.24 μmol/L.The F-PEAc1-GFP could stimulate myosinMg-ATPase activity in a protein concentration dependantmanner (about 4 folds at 1 mg/mL F-PEAc1-GFP). The re-sults above show that the PEAc1 fused to GFP retained theassembly characteristic of actin, indicating that gene fusion,prokaryotic expression, denaturation and renaturation,andaffinity chromatography is a useful strategy for obtainingplant actin isoform proteins in a large amount. | ZHANGShaobin RENDongtao XUXiaojing LIUGuoqin | 2004 | Chinese Science Bulletin2004，49，9： | 2 |
| 4 | 显示文摘 | XuXiaojing(许晓静) LiuMin(刘敏) ZhangTifeng(张体峰)etal | | 稀有金属材料与工程0，37，3： | 1 |
| 5 | 显示文摘 | XuXiaojing(许晓静) WangBin(王彬) WuGuichao(吴桂潮)etal | | 稀有金属材料与工程0，，： | 1 |
| 6 | Superplastidty of a SiC particulate-reinforced 6A02A1 matrix composite显示文摘The superplastic characteristics of a 15% (volume fraction) SiCp (14 μm)/6A02Al composite, fabricated by stir-ring casting and cold-rolling after extrusion with a low extrusion ratio of 10:1 and hot-rolling, were investigated. The com-posite exhibits a tensile elongation of 250% at an initial strain rate of 8.9×10^-4 s^-1 and at 833 K where an appropriate amount of liquid phase is presented in the composite, The superplastic deformation is uniform and the strain hardening ex-ponent γis as high as 1.6. The fracture surface is intergranual and the superplastic deformation mechanism of the composite is considered to be grain boundary (interface) sliding accommodated by an appropriate amount of liquid phase. | XUXiaojing WANGWei CAILan | 2004 | Rare Metals2004，23，1： | 1 |
| 7 | 显示文摘 | XuXiaojing(许晓静) ZhangXuefeng(张雪峰) CaoJinqi(曹进琪)eta1 | 2006 | 稀有金属材料与工程2006，35，2： | 1 |
| 8 | 显示文摘 | XuXiaojing(许晓静) WangBin(王彬) WuGuichao(吴桂潮)etal | | 稀有金属材料与工程0，，： | 1 |
| 9 | 显示文摘 | XuXiaojing(许晓静) SongTao(宋涛) FanZhen(范真)eta1 | 2012 | 稀有金属材料与工程2012，41，2： | 1 |
| 10 | 显示文摘 | SongTao(宋涛) XuXiaojing(许晓静) FanZhen(范真)eta1 | 2012 | 稀有金属材料与工程2012，41，2： | 1 |
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