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| 1 | 查看详情显示文摘 | S.H.Foulger P.Jiang Y.Ying | | 0，，： | 1 |
| 2 | Genetic population structure of red snapper(Lutjanus malabaricus)and orange-spotted grouper(Epinephelus coiodes)in Brunei and Sabah显示文摘The genetic population structure of red snapper Lutjanus malabaricus and orange-spotted grouper Epinepheluscoiodes in Brunei and Sabah was investigated using allozyme electrophoresis. Samples were collected from threesites in Brunei for E. coiodes and from three sites in Brunei and Sabah for L. malabaricus. A total of 22 loci and 16loci were scored, respectively. The index of fixation (FST) for the E. coiodes population was 0.176 but, in general, itlacked within-population structuring. The FST was particularly high between Brunei Bay/Jerudong and Brunei Bay/Kuala Belait, suggesting genetic subdivision on a small spatial scale. Isolation of Brunei Bay from the South ChinaSea may constrain the movement of adult fishes and larval dispersal, thereby reducing homogeneity amongsubpopulations. All variable loci for E. coiodes were in Hardy–Weinberg equilibrium except for MDH* and GTDHP*(P < 0.01), in which two subpopulations showed an excess of heterozygotes (P < 0.01). The study on the L.malabaricus population showed a heterozygote deficit of approximately 60% in variable loci (FIS, genetic variationwithin population = 0.45;P < 0.05);however, the mean observed heterozygosity for the population far exceeded L.malabaricus populations in Australia and Indonesia. A FST value of 0.076 revealed moderate genetic differentiationamong subpopulations of L. malabaricus. The genotypes were likely to be drawn from the same distribution inJerudong and Kuala Belait. This study infers that sustainable management of snapper and grouper resources inBrunei waters must take into account the presence of a single stock and two stocks, respectively. | Zohrah H.SULAIMAN Khadijah H.ABDUL RAHMAN Teo Y.YING Hussein H.TAHA NurQistina H.MUHD TAHA | 2008 | Integrative Zoology2008，3，3： | 0 |
| 3 | Water-soluble Cu30 nanoclusters as a click chemistry catalyst for living cell labeling via azide-alkyne cycloaddition显示文摘Cu(I)-catalyzed azide-alkyne cycloadditions(CuAAC)have gained increasing interest in the selective labeling of living cells and organisms with biomolecules.However,their application is constrained either by the high cytotoxicity of Cu(I)ions or the low activity of CuAAC in the internal space of living cells.This paper reports the design of a novel Cu-based nanocatalyst,watersoluble thiolated Cu30 nanoclusters(NCs),for living cell labeling via CuAAC.The Cu30 NCs offer good biocompatibility,excellent stability,and scalable synthesis(e.g.,gram scale),which would facilitate potential commercial applications.By combining the highly localized Cu(I)active species on the NC surface and good structural stability,the Cu30 NCs exhibit superior catalytic activities for a series of Huisgen cycloaddition reactions with good recyclability.More importantly,the biocompatibility of the Cu30 NCs enables them to be a good catalyst for CuAAC,whereby the challenging labeling of living cells can be achieved via CuAAC on the cell membrane.This study sheds light on the facile synthesis of atomically precise Cu NCs,as well as the design of novel Cu NCs-based nanocatalysts for CuAAC in intracellular bioorthogonal applications. | Ge Yang Yali Xie Yaru Wang Ying Tang Leng Leng Chng Fuyi Jiang Fanglin Du Xianfeng Zhou Jackie Y.Ying Xun Yuan | 2023 | Nano Research2023，16，1： | 0 |
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