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| 1 | Adhesion of pathogenic Vibrio alginolyticus to the gill mucus of Pseudosciaena crocea显示文摘到 Pseudosciaena croceahas 的鳃粘液的 Vibrio alginolyticus 的粘附使用调查[methyl-~3H ] thymidine 作为同位素示踪物。结果显示出那;V 的粘合剂数量。alginolyticus 与细菌的集中增加了并且在为 180 min 孵化了以后,到达了平衡;更高的粘合剂数量在 15 ~被获得 30 ℃ a nd 微酸的条件;V 的粘附。alginolyticus 不能没有 Na~+ ,完成了,并且 Ca~(2+) 在细菌的粘附起了一个辅助作用;V 的粘附。alginolyticus 被饥饿,热处理和周期的酸处理显著地禁止;所有调查的八种糖类提高了 V 的粘附。alginolyticus 到 P 的鳃粘液。crocea 在他们之中,葡萄糖,甘露糖, fructose 和麦芽糖显示出特殊提高的粘附。Theresults 显示了那 V。alginolyticus 能遵守 P 的鳃粘液。crocea 被环境因素并且仔细轻易地与表面的糖类结构和一些热敏感的结构有关在海水,和这细菌的粘附影响。 | CHEN Qiang YAN Qingpi MA Shen ZHUANG Zhixia WANG Xiaoru | 2007 | Acta Oceanologica Sinica2007，26，3： | 3 |
| 2 | Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi显示文摘Chemotactic motility is involved in the virulence of many pathogenic bacteria. In order to understand the role of chemotactic motility of Vibrio harveyi in cellular processes and virulence, mini-Tn10/Kan transposon-induced mutants with deficient chemotactic motility were constructed, screened, and identified. Sequence analysis revealed that the 465-bp fragment (GenBank accession number HM630274) flanking the transposon insertion site in mutant TS-CM1 had the highest identity (96.9%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (91.8%) with the pgk gene of V. parahaemolyticus RIMD 2210633. In another mutant, TS-CM2, 356 bp of transposon-flanking sequence (GenBank accession number HM630275) also showed the highest identity (94.6%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (92.4%) with the flaB gene of V. alginolyticus HY9901. Studies on virulence-related biological characteristics such as growth, motility, adhesion, and infectivity of the mutants showed that disruption of either the flagellin gene or energy metabolism gene led to subsequent loss of chemotactic motility and changes in growth, motility, adhesion, and virulence of the pathogenic V. harveyi. Hence, the flagellin gene and crucial energy metabolism gene played an important role in the chemotactic motility of V. harveyi. | QIN Yingxue YAN Qingpi SU Yongquan LI Haiping ZOU Wenzheng | 2013 | Acta Oceanologica Sinica2013，32，8： | 1 |
| 3 | The portal of entry for pathogenic Vibrio alginolyticus into Pseudosciaene crocea and characteristic of the bacterial adhesion to the mucus显示文摘 | CHEN QIANG YAN QINGPI WANG KEJUAN | 2008 | Disease of Aquatic Organism2008，80，： | 1 |
| 4 | Characteristics of adherence of pathogenic Vibrio alginolyticus to the intestinal mucus of large yellow croakers (Pseudosciaena crocea)显示文摘 | YAN QINGPI CHEN QIANG MA SHEN | 2007 | Aquaculture2007，269，14： | 1 |
| 5 | Starvation effects on pathogenic Vibrio alginolyticus in natural seawater显示文摘To get a better understanding of the starvation survival strategy of pathogenic Vibrio alginolyticus,log-phase cells were inoculated into sterile natural seawater for starvation studies.The results showed that all of total bacteria number,viable bacteria number and CFU number of V.alginolyticus increased remarkably at the initial starvation stage;after reaching their peaks at 5 d,both total bacteria number and viable bacteria number of V.alginolyticus fell slowly,while the CFU number fell more quickly after reaching its peak at 10 d;V.alginolyticus elongated their cells at the prophase of starvation,and then shrunk their volume and turned their shapes into ovals from rods at the anaphase of starvation;starved cells showed more sensitivity to heating and UV;starved cells showed no significant difference from unstarved ones at the lowest detection limit determined by indirect enzyme-linked immunosorbent assay(ELISA);starved cells' ability to adhere to the skin mucus of large yellow croaker(Pseudosciaena crocea)showed a sharp decline as the starvation time increases;the cellular protein of V.alginolyticus increased remarkably at the anaphase of starvation.The results indicated that pathogenic V.alginolyticus could survive in starvation for relatively long periods of time(≥2 months)in 28℃ natural seawater due to the morphological and physiological changes;however,starved V.alginolyticus cells showed less virulence and higher sensitivity under environmental stresses. | YI Jiabo CHEN Qiang ZOU Wenzheng YAN Qingpi ZHUANG Zhixia WANG Xiaoru | 2008 | Acta Oceanologica Sinica2008，27，4： | 0 |
| 6 | Antigenicity analysis of Vibrio harveyi TS-628 strain显示文摘Vibrio harveyi,the major causative agent of vib-riosis,affects a diverse range of marine cultured organisms over a wide geographical area.However,reports about screening the effective antigen and research on vaccines of V.harveyi are scarce.Flagellin,lipopolysaccharide(LPS)and outer membrane proteins(OMP)are major immunogenic antigens in many Gram-negative bacteria.In this study,the flagellin,OMP and LPS of the V.harveyi TS-628 strain iso-lated from infected groupers were extracted and Western blot analysis was used to detect the antigenicity of these extrac-tions.Results of the Western blot assay reveal that there are four positive flagellin bands:35 kDa,38 kDa,43 kDa,and 52 kDa,of which the 43 kDa and 52 kDa bands displayed the strongest positive reaction.There are five positive OMPbands about 35 kDa,38 kDa,43 kDa,47 kDa,and 52 kDa,of which the 43 kDa appeared to have the strongest positive reaction although the other four proteins also displayed strong reac-tions.However,LPS is Western blot-negative.These results indicate that the 43 kDa and 52 kDa flagellin and OMP of size 43 kDa,52 kDa can be candidates for developing vaccines against V.harveyi. | QIN Yingxue WANG Jun WANG Shifeng YAN Qingpi | 2007 | Frontiers in Biology2007，2，3： | 0 |
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