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| 1 | Fundamental CRISPR-Cas9 tools and current applications in microbial systems显示文摘Derived from the bacterial adaptive immune system,CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering.In this review,we outline the fundamental CRISPR tools that have been employed for strain optimization.These tools include CRISPR editing,CRISPR interference,CRISPR activation and protein imaging.To further characterize the CRISPR technology,we present current applications of these tools in microbial systems,including model-and non-model industrial microorganisms.Specially,we point out the major challenges of the CRISPR tools when utilized for multiplex genome editing and sophisticated expression regulation.To address these challenges,we came up with strategies that place emphasis on the amelioration of DNA repair efficiency through CRISPR-Cas9-assisted recombineering.Lastly,multiple promising research directions were proposed,mainly focusing on CRISPR-based construction of microbial ecosystems toward high production of desired chemicals. | Pingfang Tian Jia Wang Xiaolin Shen Justin Forrest Rey Qipeng Yuan Yajun Yan | 2017 | Synthetic and Systems Biotechnology2017，2，3： | 4 |
| 2 | Relationship of proprioception,cutaneous sensitivity,and muscle strength with the balance control among older adults显示文摘Background:Balance impairment is one of the strongest risk factors for falls.Proprioception,cutaneous sensitivity,and muscle strength are 3 important contributors to balance control in older adults.The relationship that dynamic and static balance control has to proprioception,cutaneous sensitivity,and muscle strength is still unclear.This study was performed to investigate the relationship these contributors have to dynamic and static balance control.Methods:A total of 164 older adults(female=89,left dominant=15,age:73.5±7.8 years,height:161.6±7.1 cm,weight:63.7±8.9 kg,mean±SD)participated in this study.It tested the proprioception of their knee flexion/extension and ankle dorsi/plantarflexion,along with cutaneous sensitivity at the great toe,first and fifth metatarsals,arch,and heel,and the muscle strength of their ankle dorsi/plantarflexion and hip abduction.The Berg Balance Scale(BBS)and the root mean square(RMS)of the center of pressure(CoP)were collected as indications of dynamic and static balance control.A partial correlation was used to determine the relationship between the measured outcomes variables(BBS and CoPRMS)and the proprioception,cutaneous sensitivity,and muscle strength variables.Results:Proprioception of ankle plantarflexion(r=-0.306,p=0.002)and dorsiflexion(r=-0.217,p=0.030),and muscle strength of ankle plantarflexion(r=0.275,p=0.004),dorsiflexion(r=0.369,p<0.001),and hip abduction(r=0.342,p<0.001)were weakly to moderately correlated with BBS.Proprioception of ankle dorsiflexion(r=0.218,p=0.020)and cutaneous sensitivity at the great toe(r=0.231,p=0.041)and arch(r=0.285,p=0.002)were weakly correlated with CoP-RMS in the anteroposterior direction.Proprioception of ankle dorsiflexion(r=0.220,p=0.035),knee flexion(r=0.308,p=0.001)and extension(r=0.193,p=0.040),and cutaneous sensitivity at the arch(r=0.206,p=0.028)were weakly to moderately correlated with CoP-RMS in the mediolateral direction.Conclusion:There is a weak-to-moderate relationship between proprioception and dynamic and static balance control,a weak relationship between cutaneous sensitivity and static balance control,and a weak-to-moderate relationship between muscle strength and dynamic balance control. | Qipeng Song Xinyan Zhang Min Mao Wei Sun Cui Zhang Yan Chen Li Li | 2021 | Journal of Sport and Health Science2021，10，5： | 2 |
| 3 | Risk factors of recurrent acute pancreatitis after conservative treatment in pediatric population: a single-center study显示文摘Background Although complete resolution and recovery occurs in most children with an initial attack of acute pancreatitis(AP),a subset of children may progress to recurrent AP(RAP).RAP has serious effects to the individual and the socioeconomic burden.The aim of this project was to identify the independent risk factors for pediatric RAP so as to provide evidence for its prevention,early diagnosis and treatment.Methods A retrospective cohort study of children discharged from Tianjin Children’s Hospital from June 2017 to January 2020 was performed.Demographic and clinical variables,treatment strategies,clinical course and outcomes were collected.Independent risk factors of RAP were identified using the logistic regression model.Results Of the total 96 enrolled children,30(31.3%)developed RAP during the follow-up period.The majority(27/30,90%)of the children with AP developed RAP within 6 months of their first AP attack.The presence of systemic inflammatory response syndrome(SIRS)[odds ratio(OR)=6.652,95%confidence interval(CI)1.989 to 22.247],fasting time(OR=1.267,95%CI 1.104 to 1.583),whether meet all three AP diagnostic criteria(OR=7.438,95%CI 1.346 to 41.103)and abnormal amylase/lipase value on the seventh day of hospitalization(OR=3.601,95%CI 0.972 to 13.342)were independent risk factors of RAP in children.Conclusions Most children who developed RAP had progressed within 6 months after their first episode of AP.RAP was more common in children who met all three AP diagnostic criteria at initial attack and in children with SIRS,long fasting time and abnormal amylase/lipase value on the seventh day of hospitalization. | Qipeng Zheng Xueqiang Yan Liang Ge Shujian Zhang Yan Sun Jinfu Jia Shengqiao Zhao Xiaodan Xu Ran Dou Qingyun Gou Jinfeng Zhao Jianghua Zhan | 2021 | World Journal of Pediatric Surgery2021，4，1： | 1 |
| 4 | Inhibition of acetate accumulation leads to enhanced production of ( R,R )-2,3-butanediol from glycerol in Escherichia coli显示文摘 | Xiaolin Shen Yuheng Lin Rachit Jain Qipeng Yuan Yajun Yan | 2012 | Journal of Industrial Microbiology & Biotechnology2012，，11： | 1 |
| 5 | SEM-Logit integration model of travel mode choice behaviors显示文摘 | CHEN Jian YAN Qipeng YANG Fei | 2013 | Journal of South China University of Technology(Natural Science Edition)2013，41，2： | 1 |
| 6 | Production of trehalose by permeabilized Micrococcus QS412 cells显示文摘 | ZHOU Yan YUAN Qipeng GAO Huiling | 2006 | Journal of Molecular Catalysis2006，43，14： | 1 |
| 7 | Text information aggregation with centrality attention显示文摘A lot of natural language processing problems need to encode the text sequence as a fix-length vector, which usually involves an aggregation process of combining the representations of all the words, such as pooling or self-attention. However, these widely used aggregation approaches do not take higher-order relationships among the words into consideration. Hence we propose a new way of obtaining aggregation weights, called eigen-centrality self-attention. More specifically, we build a fully-connected graph for all the words in a sentence, then compute the eigen-centrality as the attention score of each word. The explicit modeling of relationships as a graph is able to capture some higher-order dependency among words, which helps us achieve better results in 5 text classification tasks and one SNLI task than baseline models such as pooling, self-attention, and dynamic routing. Besides, in order to compute the dominant eigenvector of the graph, we adopt a power method algorithm to get the eigen-centrality measure. Moreover, we also derive an iterative approach to get the gradient for the power method process to reduce both memory consumption and computation requirement. | Jingjing GONG Hang YAN Yining ZHENG Qipeng GUO Xipeng QIU Xuanjing HUANG | 2021 | Science China(Information Sciences)2021，64，12： | 0 |
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