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| 1 | Dissimilatory Fe(Ⅲ) reduction characteristics of paddy soil extract cultures treated with glucose or fatty acids显示文摘Dissimilatory Fe(Ⅲ) reduction is a universal process with irreplaceable biological and environmental importance in anoxic environments. Our knowledge about Fe(Ⅲ) reduction predominantly comes from pure cultures of dissimilatory Fe(Ⅲ) reducing bacteria (DFRB). The objective of this study was to compare the effects of glucose and a selection of short organic acids (citrate, succinate, pyruvate, propionate, acetate, and formate) on Fe(Ⅲ) reduction via the anaerobic culture of three paddy soil solutions with Fe(OH)3 as the sole electron acceptor. The results showed significant differences in Fe(Ⅲ) reduction among the three paddy soil solutions and substrate types. Bacteria from the Sichuan paddy soil responded quickly to substrate supply and showed higher Fe(Ⅲ) reducing activity than the other two soil types. Bacteria in the Jiangxi paddy soil culture solution could not use propionate as a source of electrons for Fe(Ⅲ) reduction. Similarly, bacteria in the Jilin paddy soil culture could not use succinate effectively. Pyruvate was readily used by cultures from all three paddy soil solutions, thus indicating that there were some similarities in substrate utilization by bacteria for Fe(Ⅲ) reduction. The use of glucose and citrate as substrate for dissimilatory Fe(Ⅲ) reduction indicates important ecological implications for this type of anoxic respiration. | HE Jiangzhou1,2, QU Dong1, 1. College of Resources and Environment, Northwest A & F University, Yangling, Shaanxi 712100, China.2. Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar, Xinjiang Uygur Autonomous Region, Xinjiang 843300, China | 2008 | Journal of Environmental Sciences2008，20，9： | 24 |
| 2 | The EST Analysis of A Suppressive Subtraction cDNA Library of Chinese Wild Vitis pseudoreticulata Inoculated with Uncinula necator显示文摘Uncinula necator is a worldwide serious fungus disease causing annual heavy lost on grapevine production. In order to get more informations on defense related EST sequences and help breeding program, we constructed and characterized a suppression subtractive hybridization cDNA library with artificially inoculated leaves and control Chinese wild Vitis pseudoreticulata clone Baihe-35-1, which is highly resistant to powdery mildew. In the library, the length of 58 EST fragments known as putative functions varied from 130 to 800 bp, and 60% of the ESTs exhibited high similarity to known sequences in database of GenBank with BLASTX analysis. These genes were involved in stress/defense response, detoxification, signal transduction, disease defense, and etc., and 14 ESTs remained unknown or hypothetical proteins, which may be new genes. The experiment provided an important basis for studying the disease-resistance mechanism and obtaining the genes for the aim of improving grapevine powdery mildew resistance. | SHI Jiang-li, WANG Yue-jin, ZHU Zi-guo and ZHANG Chao-hong Key Laboratory of Horticultural Plant Germplasm Utilization in Northwest China, Ministry of Agriculture/Shaanxi Key Laboratory of Molecular Biology for Agriculture/College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China | 2010 | Agricultural Sciences in China2010，9，2： | 5 |
| 3 | Research on Benefit Distribution Mechanism of Farmers’ Specialized Cooperative Economic Organization显示文摘On the basis of conducting survey in Jiaonan City,the thesis analyzes benefit distribution mechanism of farmers’ specialized cooperative economic organizations:firstly,the benefit correlation is mainly the contract correlation;secondly,the profit distribution is mainly the dividend;thirdly,patronage refund has many connotations;fourthly,the public accumulation has not yet been quantified to individual;fifthly,the government support capital is ill-defined.Based on these,the measures are put forward in order to improve benefit distribution pattern of farmers’ specialized cooperative economic organizations:firstly,reform structure of property rights and realize farmers’ ownership;secondly,change mode of decision-making,and realize farmers’ control;thirdly,increase turnover rebate and realize farmers’ benefiting;fourthly,quantify public accumulation and government support fund. | SUN Hao-jie1,WANG Zheng-bing2,WANG Yun-hui3 1.School of Economics and Management,Chang’ an University,Xi’an 710064,China 2.College of Economics and Management,Northwest A & F University,Yangling 712100,China 3.Publicity Office of Party Committee,Xi’an Branch of the People’ Bank of China,Xi’an 710064,China | 2011 | Asian Agricultural Research2011，3，4： | 5 |
| 4 | GLOBAL STABILITY IN TIME-DELAYED 3-DIMENSIONAL RICHARDS MODEL显示文摘We obtain the global attractivity and global asymptotical stability of positive equilibria to a 3-dimensional Richards model with delays. Our results do not depend on the size-asymmetry parameter which measures the degree of the curvature of size-growth among individuals over the entire growth curve, and the shape parameter which affects the shape of model curve. Lastly, we gave a numerical simulation to verify the feasibility of our main results. | Lifei Zheng (College of Science, Northwest A and F University, Yangling 712100, Shaan’xi) Huiyan Zhao (College of Plant Protection, Northwest A and F University, Yangling 712100, Shaan’xi) | 2010 | Annals of Differential Equations2010，26，1： | 3 |
| 5 | Effects of Microtubule Polymerization Inhibitor on the Hypersensitive Response of Wheat Induced by the Non-Host Pathogen Sphaerotheca fuliginea显示文摘The plant cytoskeleton is a highly dynamic and versatile intracellular scaffold composed of microtubules and microfilaments, serving a multiplicity of functions in plant cells. To reveal the relationship between the cytoskeleton in wheat (Triticum aestivum L.) cv. Suwon 11 attacked by the non-host pathogen Sphaerotheca fuliginea and the initiation of the hypersensitive response, the microtubule inhibitor oryzalin was injected into the wheat leaves immediately prior to inoculation. The incidence of hypersensitive cell death was significantly lower than that in water-treated control. In addition, the occurrence of hypersensitive cell death was also delayed and S. fuliginea was able to penetrate and form haustoria in epidermal tissues of wheat. All the results above indicated that hypersensitive cell death was associated with depolymerisation of microtubules, suggesting that microtubules might play an important role in the expression of non-host resistance of wheat. | LI Hong-li, WANG Hai-yan, HAO Xin-yuan, SONG Xiao-he and MA Qing College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University/Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Yangling 712100, P.R.China | 2010 | Agricultural Sciences in China2010，9，3： | 3 |
| 6 | Using soil surface gray level to determine surface soil water content显示文摘How to determine surface soil water content(SWC) quickly and accurately is fundamental in studying eco-hydrological processes and their modeling.Here we use laboratory experiments to determine surface SWC using soil surface gray level(SGL) values.A negatively exponential relationship exists between SGL and SWC,i.e.,SGL increases with the decrease of SWC.SGL can be estimated based on initial SGL value(surface gray level when SWC=0),SWC,and a surface roughness coefficient characterized by mean soil particle size.The variation range of SGL was larger than that of SWC,indicating that changes in SWC were enhanced in SGL,and that SGL would thus be sensitive to changes in soil water.At the 95% confidence level,SWC can be determined by using the relationship between SWC and SGL established by the experiments.The determination of SWC has a high precision when SWC was between dry and saturated. | ZHU YuanJun1,2,WANG YunQiang1,2 & SHAO MingAn1,2 1 State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau,Institute of Soil and Water Conservation,Northwest A & F University,Yangling 712100,China 2 Institute of Soil and Water Conservation,Chinese Academy of Sciences & Ministry of Water Resources,Yangling 712100,China | 2010 | Science China Earth Sciences2010，53，10： | 2 |
| 7 | Estimation and pmjection of the national profile of cancer mortality in China: 1991- 2005显示文摘 | YangL Park in DM B ray F | 2004 | Br Cancer2004，90，11： | 1 |
| 8 | Sensitivity to oxidative stress in DJ-1-deficient dopa-mine neurons:an ES-derived cell model of primary Parkinson-ism显示文摘 | Martinat C Shendelman S Jonason A Leete T Beal M F YangL | 2004 | PLoS Biol2004，2，： | 1 |
| 9 | An algorithm study on inverse identification of interfacial configuration in a multiple region domain 显示文摘 | Fan C L Sun F R YangL | 2009 | ASME Journal of Heat Transfer2009，131，02： | 1 |
| 10 | Impact of VKORCI gene polymerphism on interindividual and interethnic warfarin dosage requirement:a systematic review and meta analysis显示文摘 | YangL Ge W Yu F | 2010 | Thromb Res2010，125，： | 1 |
| 11 | cDNA Cloning of Goat DNA Methyltransferase 1,Screening of shRNA Vectors and Influences to Development of Nuclear Transfer Embryos显示文摘This study was designed to clone cDNA of goat DNA methyltransferase 1(DNMT1) gene,to screen an effective shRNAproducing vector targeting goat DNA methyltransferase 1 and to improve the developmental competence of goat nuclear transfer embryos by decreasing the DNMT1 expression in donor cells.In this study,PCR primers were designed against regions of high homology between bovine and sheep sequences and then used to amplify the larger portions of the coding regions.Next,3 RNAi oligonucleotides were designed based on the cloned sequences and inserted into pRNAT-U6.1/Neo vector,acquiring 3 new vectors,respectively termed pRNAD1,pRNAD2 and pRNAD3.Then the positive cells were sorted by flow cytometry after transfection and detected by real-time PCR analysis and sodium bisulfite genomic sequencing.Finally,the developmental rates of nuclear transfer(NT) embryos generated using donor cells with and without the effective shRNA vector respectively,as well as in vitro fertilization(IVF) embryos were observed and recorded.The results showed that the coding regions of goat DNA methyltransferase 1 gene was successfully cloned(GenBank no.FJ617538).Furthermore,an effective interfering shRNA(pRNAD2) was obtained,with its interference effect being 47.88%.Finally,NT embryos with shRNA vector harbored better developmental competence during morula and blastocyst stage compared to controls(P < 0.05),reaching the similar rates to IVF embryos(P > 0.05).In conclusion,goat DNA methyltransferase 1 gene cDNA was cloned and sequenced,an effective shRNA vector responsible for inhibiting DNA methyltransferase 1 expression was developed and the developmental competence of goat nuclear transfer morulae and blastcysts was significantly improved,which provided a feasible pathway for improving goat nuclear transfer embryo development competence by decreasing the methylation level in donor cells through RNAi-mediated manner. | LAN Jie,SONG Yong-li,HUA Song,LIU Yong-gang,LIU Jun,ZHANG Hai-lin and ZHANG Yong Key Laboratory of Animal Reproductive Physiology & Embryo Technology,Institution of Biotechnology,College of Veterinary Medicine,Northwest A&F University,Yangling 712100,P.R.China | 2010 | Agricultural Sciences in China2010，9，7： | 1 |
| 12 | Optimal control of computer virus under a delayed model 显示文摘 | ZhuQ Y YangX F YangL X | 2012 | Applied Mathematics and Computation2012，218，11： | 1 |
| 13 | An X-Y paint set and sperm FISH protocol that can be used for validation of cattle sperm separation procedures 显示文摘 | RENS W YANGL F WELCH G | 2001 | Reproduction2001，121，： | 1 |
| 14 | Corrosion be- havior of die-cast AZ91D magnesium alloy in aque- ous sulphate solutions显示文摘 | YangL J Wei Y H Hou L F | 2010 | Corrosion Science2010，52，2： | 1 |
| 15 | Cloning and Sequence Analysis of the Full-Length Genome of Japanese encephalitis virus Strain SXBJ07 Isolated from Swine显示文摘A virus strain,showing cytopathic effect in BHK-21 cell,was isolated from swine brains in Shaanxi Province,China,in 2007.The isolate was confirmed as Japanese encephalitis virus(JEV) by immunofluorescence assay(IFA) and reverse transcription polymerase chain reaction(RT-PCR),and named SXBJ07.The complete nucleotide and deduced amino acid sequences of the JEV strain SXBJ07 were determined.Its single open reading frame has a total of 3 432 amino acid residues.An extensive E gene based phylogenetic analysis was performed,the result showed that SXBJ07 strain belongs to genotype I.Comparison of the SXBJ07 genomic sequence with those of the 24 fully sequenced JEV strains in published databases showed nucleotide homology ranging from 99.0 to 83.7%;amino acid homology ranged from 99.8 to 94.8%.Compared SXBJ07 with SA14-14-2 strain,the current live vaccine strain in China,the homology of amino acid in envelope gene was 97.0%;and there were amino acid substitutions in 13 sites of the active domains of E protein(E1-E411). | WANG Wei-hua,ZHANG Yan-ming,XU Xin-gang,XING Fu-shan and TANG Qing-hai College of Veterinary Medicine,Northwest A&F University,Yangling 712000,P.R.China | 2009 | Agricultural Sciences in China2009，8，11： | 0 |
| 16 | Construction of the Government Support and Evaluation Index System of Farmers' Cooperative Economic Organizations显示文摘On the basis of expounding the necessity of evaluation index system of farmers' cooperative economic organization,the three first level indexes including organization development index,member development index and social development index and 13 second level indexes including aggregate capital of cooperative organization,annual profit share of cooperative organization,annual total income of cooperative organization,etc.,are established according to the principles of Systematics and the methods of Statistics and Quantitative economics.By using the analytical hierarchy process,the weight of each index is tested and the operation result and evaluation mode is established,as well as the index standard value and comprehensive value.As for the result of the evaluation,it is suggested that the government should support the farmers' cooperative organizations in hierarchy and targeted way according to local conditions.Though the construction of index system and comprehensive score,the government should establish the entry and exit mechanism of farmers' cooperative organizations to reasonably guild the rapid development of cooperators. | SUN Hao-jie1,WANG Zheng-bing2,WANG Yun-hui3 1.School of Economics and Management,Chang’an Univerity,Xi’an 710064,China 2.College of Economics and Management,Northwest A&F University,Yangling 712100,China 3.Department of Publicity,Xi’an Branch of Bank of China,Xi’an 710075,China | 2011 | Asian Agricultural Research2011，3，5： | 0 |
| 17 | Expression of EGF and EGFR in Developing Skin of the Goat Fetus显示文摘The histological characteristics,distribution and variability of epidermal growth factor(EGF) and epidermal growth factor receptor(EGFR) during development of skin in the goat fetus were studied with histological and immunohistochemical methods.The results showed that the epidermis formed at the 6th gestational week,the thickness increased gradually because of transition from simple epithelium to stratified epithelium,and then decreased after the 15th week.The dermis was present at the 10th week,and the dermal derivatives appeared after the 11th week.All structures of the skin matured gradually during prenatal development.Low expression of EGF and EGFR could be observed at the 6th week and increased thereafter.Before the 11th week,EGF was mainly located in the cytoplasm of the basal layer of cells of epidermis,hair follicular epithelial cells and fibroblasts,and EGFR was mainly located on cell membranes of these cells.From the 11th to 16th week,the expression of EGF and EGFR extended from basal layer cells,prickle cells,hair follicular epithelial cells and fibroblasts to vascular endothelial cells,epithelial cells of sweat glands and arrector pili muscle.EGF was mainly detected cytoplasmically,and EGFR was located on membranes of these cells.From the 17th week to birth,EGF was mainly located in cells of the basal layer and hair follicular epithelial cells as the skin thinned and staining intensity continued to increase.The expressed amount of EGF and EGFR tended to increase throughout the whole development stage encompassed by gestational weeks 6 to 21 and the two components of the EGF signaling system were highly correlated.The results suggest that EGF and EGFR play important roles in the development of skin and its derivatives. | QING Su-zhu,LIN Ji-mao,ZHANG Ling-zhi,WANG Lei-lei,LUO Shi-shuang (College of Veterinary Medicine,Northwest A & F University,Yangling 712100,China) | 2010 | 畜牧兽医学报2010，41，S1： | 0 |
| 18 | NONDENSELY DEFINED IMPULSIVE NEUTRAL FUNCTIONAL DIFFERENTIAL INCLUSIONS WITH NONLOCAL CONDITIONS显示文摘In this paper,using a fixed point theorem for condensing multi-valued maps,we investigate the existence of integral solutions to a class of nondensely defined neutral evolution impulsive differential inclusions with nonlocal conditions in Banach spaces. | Yueju Cao (College of Science,Northwest A&F University,Yangling 712100,Shanxi) Xianlong Fu (Dept. of Math.,East China Normal University,Shanghai 200062) | 2009 | Annals of Differential Equations2009，25，2： | 0 |
| 19 | Factors Influencing Willingness and Ability of Farmers to Adopt New Technologies:A Case Study of Guanzhong Area显示文摘Based on data of 248 rural households in Pucheng County and Huxian County,we established the Two-Level Logit Model to analyze the willingness of farmers to adopt new technologies,its influence factors,and probability of successful adoption of new technologies.Results show that the willingness has positive correlation with whether the farmer is head of household,the educational level,occupation,agricultural loan,the number of family labor,and information dissemination channel,while it has negative correlation with non-agricultural employment proportion and whether the farmer is village cadre.In the model of the probability of farmers'successfully adopting new technologies,occupation,agricultural loan,planting area,gender and educational level are positively correlated,while age and non-agricultural employment proportion are negatively correlated.Largescale flow of rural labor plays a negative role in popularization of technologies in rural areas through influencing factors,including number of family labor,non-agricultural employment proportion,educational level,gender,and whether the farmer is village cadre.Finally,on the basis of results of empirical study,we put forward countermeasures and suggestions for strengthening ability of farmers to adopt new technologies. | TIAN Jing-na,LI Lu-tang College of Economics and Management,Northwest A&F University,Yangling 712100,China | 2012 | Asian Agricultural Research2012，4，7： | 0 |
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