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| 1 | Identification and functional annotation of novel microRNAs in the proximal sciatic nerve after sciatic nerve transection显示文摘The peripheral nervous system is able to regenerate after injury, and regeneration is associated with the expression of many genes and proteins. MicroRNAs are evolutionarily conserved, small, non-coding RNA molecules that regulate gene expression at the level of translation. In this paper, we focus on the identification and functional annotation of novel microRNAs in the proximal sciatic nerve after rat sciatic nerve transection. Using Solexa sequencing, computational analysis, and quantitative reverse transcription PCR verification, we identified 98 novel microRNAs expressed on days 0, 1, 4, 7, and 14 after nerve transection. Furthermore, we predicted the target genes of these microRNAs and analyzed the biological processes in which they were involved. The identified biological processes were consistent with the known time-frame of peripheral nerve injury and repair. Our data provide an important resource for further study of the role and regulation of microRNAs in peripheral nerve injury and regeneration. | LI ShiYing YU Bin WANG YongJun YAO DengBing ZHANG ZhanHu GU XiaoSong | 2011 | Science China(Life Sciences)2011,54,9: | 16 |
| 2 | Facile fabrication of dolomite-doped biochar/bentonite for effective removal of phosphate from complex wastewaters显示文摘The removal of phosphate from wastewater using traditional biological or precipitation methods is a huge challenge.The use of high-performance adsorbents has been shown to address this problem.In this study,a novel composite adsorbent,composed of dolomite-doped biochar and bentonite(DO/BB),was first synthesized via co-pyrolysis.The combination of initial phosphate concentration of 100 mg/L and 1.6 g/L of DO/BB exhibited a high phosphate-adsorption capacity of 62 mg/g with a removal efficiency of 99.8%.It was also stable in complex water environments with various levels of solution pH,coexisting anions,high salinity,and humic acid.With this new composite,the phosphate concentration of the actual domestic sewage decreased from 9 mg/L to less than 1 mg/L,and the total nitrogen and chemical oxygen demand also decreased effectively.Further,the cross-flow treatment using a PVC membrane loaded with DO/BB(PVC-DO/BB),decreased the phosphate concentration from 1 to 0.08 mg/L,suggesting outstanding separation of phosphate pollutants via a combination of adsorption and separation.In addition,the removal of phosphate by the PVC-DO/BB membrane using NaOH solution as an eluent was almost 90%after 5 cycles.The kinetic,isotherm and XPS analysis before and after adsorption suggested that adsorption via a combination of electrostatic interaction,complexation and precipitation contributed to the excellent separation by the as-obtained membranes. | Huan Xi Fanlu Min Zhanhu Yao Jianfeng Zhang | 2023 | Frontiers of Environmental Science & Engineering2023,17,6: | 0 |
| 3 | Electroless Plating of Ni Nanoparticles on WC to Assist Its Pressureless Sintering of WC-Ni Cemented Carbide with Enhanced Mechanical and Corrosion-resistant Performance显示文摘Ni nanoparticles were coated uniformly on the surface of WC powder via a facile electroless plating method(abbreviated as WCN-EP),and then consolidated for mechanical and corrosion resistance performance characterization,in comparison with hand mixed WC-Ni(WCN-H).Under the optimized electroless plating parameters,Ni particles,less than 1μm in average diameter,were found to be uniformly and densely wrapped on the surface of the tungsten carbide matrix of WCN-EP.In comparison,in WCN-H,the Ni particles about 1.8μm in average diameter,were randomly distributed together with irregular WC particles.The uniform coating of Ni was found to assist the densification process of WCN-EP effectively,with higher densities and less pores than those of WCN-H at the Ni content of 10.6wt%,25.5wt%,and 30.3 wt%.However,at the Ni content of 18.8wt%,the relative densities of WCN-EP and WCN-H both increased to the maximum value of 98%.The maximum hardness of the consolidated WCN-EP was 82.6 HRA,about 1.2 HRA higher than that of WCN-H.In addition,the consolidated WCN-EP also exhibits a superior corrosion resistance by the polarization curve analysis at an electrochemical workstation. | 闵凡路 YANG Hao YAO Zhanhu LI Xinggao 张建峰 LIU Hai | 2021 | Journal of Wuhan University of Technology(Materials Science)2021,36,6: | 0 |
| 4 | Comparative Study on Microstructure and Mechanical Properties of Coarse-grained WC-based Cemented Carbides Sintered with Ultrafine WC or (W+C) as Additives显示文摘The effects of ultrafine WC(WC_(UF),0.5μm) or W(1μm) and C(0.3μm)(W+C)_(UF) additives on the densification,microstructure and mechanical properties of coarse-grained cemented carbides were compared systematically.Overall,the cemented carbides with WC_(UF)/(W+C)_(UF) additives are almost fully densification to be higher than 99%,and the average grain size is kept above 2.8μm.The WC_(UF) additive assists grains to(truncated)trigonal prism shape by two dimensional(2D) growth,whereas the(W+C)_(UF) additive assists grains to rounded shape by three dimensional(3D) growth,lowers WC contiguity and increases face-centered-cubic Co.The hardness and bending strength of(75WC_(C)-15WC_(UF))-10Co are 86.6 HRA and 2 272 MPa,respectively,both higher than those of(75WC_(C)-15(W+C)_(UF))-10Co,which could be ascribed to the enhanced densification and unblemished grains.However,the fracture toughness of the(75WC_(C)-15(W+C)_(UF))-10Co is 23.5 MPa·m^(1/2),higher than that of the(75WC_(C)-15WC_(UF))-10Co due to the uniform WC-Co structure and flexible binder phase. | 于淞百 闵凡路 LI De NOUDEM Guillaume Jacques ZHANG Hailong MA Jichang ZHAO Kui YAO Zhanhu 张建峰 | 2024 | Journal of Wuhan University of Technology(Materials Science)2024,39,2: | 0 |