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| 1 | 退化沙质草地自然恢复过程中土壤一植物系统的变化特征显示文摘研究了科尔沁沙地封育5a后退化沙质草地自然恢复过程中土壤—植物系统的变化特征,同时分析了土壤性状与植物特征之间的相互影响程度。(1)从流动沙地恢复到固定沙地,沙质草地植物群落的物种多样性、植被盖度、地下根量和地表枯落物残留量均趋于增大。β多样性测度值表明,在半流动沙地恢复到半固定沙地阶段,植物种周转速率大,群落结构变化明显。(2)在退化沙质草地的自然恢复过程中,表层土壤的结构性能明显改善,土壤养分的有效性相应提高。(3)相关分析显示,沙质草地表层土壤的理化性状与植物特征值之间存有密切的相关性(R2=0.33~0.84,P<0.01,n=20),表明退化沙质草地的恢复过程实质上是土壤—植物系统协同演变的过程,在该过程中,植物多样性的关键恢复期滞后于土壤环境的关键恢复期。 | 张华 伏乾科 李锋瑞 Yasuhito Shirato | 2003 | 水土保持通报2003，23，6： | 26 |
| 2 | Arabidopsis STAY-GREEN2 Is a Negative Regulator of Chlorophyll Degradation during Leaf Senescence显示文摘叶绿素(Chl ) 降级在老朽期间或在压力条件下面引起叶 yellowing。为 Chl 故障, STAY-GREEN1 (SGR1 ) 在 thylakoid 膜与 Chl 分解代谢的酶(CCE ) 和轻收获的复杂 II (LHCII ) 交往, possiblyto 允许新陈代谢的 channeling 潜在地 phototoxic Chl 故障中介。在这些 Chl 分解代谢的部件之中, SGR1 充当叶 yellowing 的一个关键管理者。除了 SGR1 (At4g22920 ) , Arabidopsis thaliana 染色体包含一个另外的相当或相同的事物, SGR2 (At4g11910 ) ,谁的生物功能遗体逃犯。在导致老朽的条件下面, SGR2 表示是高度起来调整的,同样到 SGR1 表示。这里,我们证明那 SGR2 功能在叶 Chl 降级抵抗 SGR1 活动;SGR2-overexpressing 植物保持绿色和 sgr2-1 大美人异种展出了早叶 yellowing 未成年 -- ,黑暗 -- ,并且导致压力的老朽条件。象 SGR1 一样,有 LHCII 的 SGR2interacted 但是与 SGR1 相对照,和 CCE 的 SGR2 相互作用很被限制。而且, SGR1 和 SGR2formed 人 -- 或 heterodimers,强烈在否定地调整 Chl 降级由为 SGR2 建议一个角色可能 interferingwith SGR1 的建议招募 CCE 功能。我们的数据在 Arabidopsis 显示 SGR1 andSGR2 的函数的对抗进化与拆除在叶绿体平衡 Chl 分解代谢并且在 senescingleaf cells.Key 词另外的细胞的部件重新动员: | Yasuhito Sakuraba So-Yon Park Ye-Sol Kim Seung-Hyun Wang Soo-Cheul Yoo Stefan Hortensteiner Nam-Chon Paek | 2014 | Molecular Plant2014，7，8： | 15 |
| 3 | Present status of endoscopic mastectomy for breast cancer显示文摘Endoscopy is now being used for breast cancer surgery. Though it is used for mastectomy, lymph node dissection, and breast reconstruction, its prime use is for mastectomy. Because an incision can be placed inconspicuously in the axillary site, a relatively largeincision can be created. A retractor with an endoscope, CO2, and an abrasion device with the endoscope are used for operation space security. It is extremely rare that an endoscope is used for lymph node dissection. For breast reconstruction, it may be used for latissimus muscle flap making, but an endoscope is rarely used for other reconstructions. Endoscopic mastectomy is limited to certain institutions and practiced hands, and it has not been significantly developed in breast cancer surgery. On the other hand, endoscopic surgery may be used widely in breast reconstruction. With respect to the spread of robotic surgery, many factors remain uncertain. | Tetsuhiro Owaki Yuko Kijima Heiji Yoshinaka Munetsugu Hirata Hiroshi Okumura Simiya Ishigami Yasuhito Nerome Toshiro Takezaki Shoji Natsugoe | 2015 | World Journal of Clinical Oncology2015，6，3： | 15 |
| 4 | 科尔沁沙地人工杨树林生态服务效能评价显示文摘采用定位观测法,系统评价了杨树(脚Populus smonii)林的防风、抗蚀和滞尘等生态服务效能及其间接价值,同时定量探讨了风速减弱系数与实测林地叶面积指数的关系.结果表明,在研究区主害风(西北风)天气下,林地迎风区6 H(H为平均树高)、3 H、林地中央、林地背风区林缘、6和8 H处2 m高度的日平均风速与对照点(流动沙丘)相比均有不同程度减弱,风速减弱系数在18.3%~66.2%之间.林地背风区6 H处0.25、0.5、1和2 m 4个高度的月平均风速减弱系数与林地叶面积指数呈显著非线性相关,其间存在良好的三次曲线关系(P<0.0001,R2=0.43~0.94,n=80).在主害风天气下,林地各观测点的地表日风蚀量与对照点相比大幅度降低,平均降幅85.2%~99.9%.在观测期内,林地中央的日平均降尘量为13.2 kg·hm-2,而林地迎风区6H处的日平均降尘量为9.9 kg·hm-2,林地的日滞尘能力约为3.3 kg·hm-2. | 张华 李锋瑞 张铜会 赵丽娅 Yasuhito Shirato | 2003 | 应用生态学报2003，14，10： | 14 |
| 5 | Inflammation-based factors and prognosis in patients with colorectal cancer显示文摘Several parameters for predicting survival in patients with colorectal cancer have been identified, including the performance status, age, gender and tumor-nodemetastasis(TNM) stage. Although the TNM stage is important and useful for predicting the prognosis and determining the appropriate treatment, it is well known that the survival time varies widely, even in patients with the same stage of disease. Therefore, the identification of new parameters capable of more precisely predicting patient survival is needed to help select the optimal treatment, especially in patients in the advanced stage of disease. Although the TNM stage reflects the tumor characteristics, cancer progression and survival are not determined solely based on the local characteristics of the tumor, but also the host systemic immune/inflammatory response. Therefore, using a combination of parameters that reflect both tumor characteristics and the host systemic inflammatory status is thought to be important for accurately predicting patient survival. | Kiyoshi Maeda Masatusne Shibutani Hiroshi Otani Hisashi Nagahara Tetsuro Ikeya Yasuhito Iseki Hiroaki Tanaka Kazuya Muguruma Kosei Hirakawa | 2015 | World Journal of Gastrointestinal Oncology2015，7，8： | 12 |
| 6 | 四川省雅江县藏区人群绦/囊尾蚴病流行现状显示文摘目的了解四川省雅江县呷拉乡藏族人群绦虫病/囊尾蚴病流行现状。方法通过问卷、体检了解近一年内人群排绦虫史、癫痫发作史、皮下结节以及相关的其他临床表现。对自愿接受治疗的患者用吡喹酮驱除绦虫,用multiplex PCR对虫种进行鉴定。对1例高度怀疑为猪带绦虫患者的粪便标本采用粪-PCR进行检测。用囊液抗原检测血清囊尾蚴病特异性抗体,了解人群囊尾蚴感染水平。结果11.96%(216/1806)的村民近一年内有排绦虫史,1.05%(19/1806)发现皮下结节,0.61%(11/1806)有癫痫发作史。24份虫体标本PCR鉴定结果均为牛带绦虫,1份粪便标本粪-PCR结果为猪带绦虫。4.35%(4/92)人群血清囊尾蚴病抗体阳性。结论牛带绦虫和猪带绦虫共同流行于雅江县呷拉乡,以牛带绦虫为优势虫种。人群囊尾蚴感染率为4.35%。 | 阿斗塔 李调英 陈兴旺 邱加闽 龙昌平 Akira Ito 邱东川 Philip S.Craig Yasuhito Sako | 2007 | 寄生虫病与感染性疾病2007，5，2： | 12 |
| 7 | T1ρ/T2 mapping and histopathology of degenerative cartilage in advanced knee osteoarthritis显示文摘AIM To investigate whether normal thickness cartilage in osteoarthritic knees demonstrate depletion of proteoglycan or collagen content compared to healthy knees.METHODS Magnetic resonance(MR) images were acquired from5 subjects scheduled for total knee arthroplasty(TKA)(mean age 70 years) and 20 young healthy control subjects without knee pain(mean age 28.9 years). MR images of T1ρ mapping, T2 mapping, and fat suppressed proton-density weighted sequences were obtained.Following TKA each condyle was divided into 4 parts(distal medial, posterior medial, distal lateral, posterior lateral) for cartilage analysis. Twenty specimens(bone and cartilage blocks) were examined. For each joint,the degree and extent of cartilage destruction was determined using the Osteoarthritis Research Society International cartilage histopathology assessment system.In magnetic resonance imaging(MRI) analysis, 2 readers performed cartilage segmentation for T1ρ/T2 values and cartilage thickness measurement.RESULTS Eleven areas in MRI including normal or near normal cartilage thickness were selected. The corresponding histopathological sections demonstrated mild to moderate osteoarthritis(OA). There was no significant difference in cartilage thickness in MRI between control and advanced OA samples [medial distal condyle, P = 0.461;medial posterior condyle(MPC), P = 0.352; lateral distal condyle, P = 0.654; lateral posterior condyle, P = 0.550],suggesting arthritic specimens were morphologically similar to normal or early staged degenerative cartilage.Cartilage T2 and T1ρ values from the MPC were significantly higher among the patients with advanced OA(P= 0.043). For remaining condylar samples there was no statistical difference in T2 and T1ρ values between cases and controls but there was a trend towards higher values in advanced OA patients. CONCLUSION Though cartilage is morphologically normal or near normal, degenerative changes exist in advanced OA patients. These changes can be detected with T2 and T1ρ MRI techniques. | Benjamin S Kester Philip M Carpenter Hon J Yu Taiki Nozaki Yasuhito Kaneko Hiroshi Yoshioka Ran Schwarzkopf | 2017 | World Journal of Orthopedics2017，8，4： | 12 |
| 8 | 四川省凉山彝族地区囊尾蚴病血清学调查(英文)显示文摘目的了解四川省凉山州彝族地区囊尾蚴病感染情况和流行风险因素。方法于2006年对凉山州昭觉县比尔乡的部分居民问卷调查;猪带绦虫尾蚴GPs作为抗原,用ELISA方法检测人、猪血清猪囊尾蚴特异性抗体。结果当地居民继发型癫痫发作率为9.1%(57/626),人、猪血清阳性率分别为7.2%(36/499)、13.9%(14/101)。该病在当地流行的主要原因包括猪放养、不良食肉习惯(坨坨肉)、人无厕所、肉品检疫不严以及经济落后等。结论囊尾蚴病高度流行于四川省彝族地区。为控制该病的流行,必需采取综合性的防治措施,如主动的人群绦虫病筛查和治疗、加强肉食品检疫和人粪管理、改变不良饮食习惯、提倡猪圈养等。 | 陈兴旺 李调英 Akira Ito Yasuhito Sako 邱东川 肖宁 Philips.Craig | 2010 | 寄生虫病与感染性疾病2010，8，2： | 11 |
| 9 | Mutation of Oryza sativa CORONATINE INSENSITIVE 1b(OsCOI1b) delays leaf senescence显示文摘Jasmonic acid(JA) functions in plant development,including senescence and immunity. Arabidopsis thaliana CORONATINE INSENSITIVE 1 encodes a JA receptor and functions in the JA-responsive signaling pathway. The Arabidopsis genome harbors a single COI gene, but the rice(Oryza sativa) genome harbors three COI homologs, Os COI1 a,Os COI1 b, and Os COI2. Thus, it remains unclear whether each Os COI has distinct, additive, synergistic, or redundant functions in development. Here, we use the oscoi1b-1 knockout mutants to show that Os COI1 b mainly affects leaf senescence under senescence-promoting conditions. oscoi1b-1 mutants stayed green during dark-induced and natural senescence,with substantial retention of chlorophylls and photosynthetic capacity. Furthermore, several senescence-associated genes were downregulated in oscoi1b-1 mutants, including homologs of Arabidopsis thaliana ETHYLENE INSENSITIVE 3 and ORESARA 1, important regulators of leaf senescence. These results suggest that crosstalk between JA signaling and Resea ethylene signaling affects leaf senescence. The Arabidopsis coi1-1 plants containing 35S:Os COI1 a or 35S:Os COI1 b rescued the delayed leaf senescence during dark incubation, suggesting that both Os COI1 a and Os COI1 b are required for promoting leaf senescence in rice. oscoi1b-1 mutants showed significant decreases in spikelet fertility and grain weight,leading to severe reduction of grain yield, indicating that Os COI1-mediated JA signaling affects spikelet fertility and grain filling. | Sang-Hwa Lee Yasuhito Sakuraba Taeyoung Lee Kyu-Won Kim Gynheung An Han Yong Lee Nam-Chon Paek | 2015 | Journal of Integrative Plant Biology2015，57，6： | 9 |
| 10 | Prognostic significance of perioperative tumor marker levels in stage Ⅱ/Ⅲ gastric cancer显示文摘AIM To evaluate the prognostic significance of perioperative carcinoembryonic antigen(CEA) and carbohydrate antigen 19-9(CA19-9) levels in stage Ⅱ/Ⅲ gastric cancer.METHODS From a multi-institutional retrospective database compiled by integrating clinical data from nine institutions, data of 998 patients who underwent curative resection for stage Ⅱ/Ⅲ gastric cancer between 2010 and 2014 were retrieved and analyzed. The prognostic impact of the preoperative and postoperative levels and chronological changes in CEA, CA19-9 and their combination were evaluated. To test whether postoperative adjuvant chemotherapy alters the prognostic impact of perioperative CEA and CA19-9 levels, the hazard ratios for mortality were compared between patients who underwent surgery alone and patients who underwent surgery followed by adjuvant chemotherapy.RESULTS The prognostic impact of postoperative CEA and CA19-9 was superior to that of the preoperative levels. Multivariable analysis identified high postoperative CEA and CA19-9 levels as independent prognostic factors for overall survival.Disease-free survival rates clearly decreased in a stepwise manner in association with postoperative CEA and CA19-9 levels, and patients with high levels of both markers showed significantly poorer prognosis than other patient groups. When we analyzed perioperative changes in serum CEA and CA19-9 levels, patients with high levels before and after surgery had the worst disease-free survival rates among all patient groups. Patients with normalized CEA levels after surgery had a significantly lower disease-free survival rate than those with normal perioperative levels, whereas patients with normalized CA19-9 levels after surgery had equivalent survival to those with normal perioperative levels. The prognostic impact of high CEA levels was observably smaller in patients who underwent adjuvant chemotherapy than in patients who underwent surgery alone, whereas that of high CA19-9 was greater in patients who underwent adjuvant chemotherapy. High postoperative CEA levels were significantly associated with an increased prevalence of liver, lung and bone recurrences, and high postoperative CA19-9 levels were significantly associated with increased frequencies of lymph node and liver recurrences.CONCLUSION The evaluation of serum CEA and CA 19-9 levels both before and after surgery provides useful information for precise risk stratification after curative gastrectomy. | Yasuhito Suenaga Mitsuro Kanda Seiji Ito Yoshinari Mochizuki Hitoshi Teramoto Kiyoshi Ishigure Toshifumi Murai Takahiro Asada Akiharu Ishiyama Hidenobu Matsushita Chie Tanaka Daisuke Kobayashi Michitaka Fujiwara Kenta Murotani Yasuhiro Kodera | 2019 | World Journal of Gastrointestinal Oncology2019，11，1： | 7 |
| 11 | Incidence and characteristics of HBV reactivation in hematological malignant patients in south Egypt显示文摘AIM:To investigate characteristics of hepatitis B virus(HBV)implicated in HBV reactivation in patients with hematological malignancies receiving immunosuppressive therapy.METHODS:Serum samples were collected from 53 patients with hematological malignancies negative for hepatitis B surface antigen(HBsAg)before the start of and throughout the chemotherapy course.HBV reactivation was diagnosed when the HBsAg status changed from negative to positive after the initiation of chemotherapy and/or when HBV DNA was detected by realtime detection polymerase chain reaction(RTD-PCR).For detecting the serological markers of HBV infection,HBsAg as well as antibodies to the core antigen(antiHBc)and to the surface antigen were measured in the sera by CEIA.Nucleic acids were extracted from sera,and HBV DNA sequences spanning the S gene were amplified by RTD-PCR.The extracted DNA was further subjected to PCR to amplify the complete genome as well as the specific genomic sequences bearing the enhancerⅡ/core promoter/pre-core/core regions(nt1628-2364).Amplicons were sequenced directly.RESULTS:Thirty-five(66%)of the 53 HBsAg-negative patients were found to be negative serologically for antiHBc,and the remaining 18(34%)patients were positive for anti-HBc.Five of the 53(9.4%)patients with hematologic malignancies experienced HBV reactivation.Genotype D1 was detected in all five patients.Four types of mutant strains were detected in the S gene product of HBV strains and were isolated from 3 patients with HBV reactivation:T/S120,L143,and I126.HBV DNA was detected in the pretreatment HBsAg-negative samples in one of the five patients with HBV reactivation.In this patient,sequences encompassing the HBV full genome obtained from sera before the start of chemotherapy and at the time of de novo HBV hepatitis were detected and it showed 100%homology.Furthermore,in the phylogenetic tree,the sequences were clustered together,thereby indicating that this patient developed reactivation from an occult HBV infection.CONCLUSION:Past infection with HBV is a risk factor for HBV reactivation in Egypt.Mandatory anti-HBc screening prior to chemotherapy in patients with hematological malignancies is recommended. | Abeer Elkady Sahar Aboulfotuh Elsayed Mostafa Ali Douaa Sayed Nashwa M Abdel-Aziz Amany M Ali Shuko Murakami Sayuki Iijima Yasuhito Tanaka | 2013 | World Journal of Gastroenterology2013，19，37： | 7 |
| 12 | Utility of tricalcium phosphate and osteogenic matrix cell sheet constructs for bone defect reconstruction显示文摘AIM: To determine the effects of transplanting osteogenic matrix cell sheets and beta-tricalcium phosphate(TCP) constructs on bone formation in bone defects.METHODS: Osteogenic matrix cell sheets were prepared from bone marrow stromal cells(BMSCs), and a porous TCP ceramic was used as a scaffold. Three experimental groups were prepared, comprised of TCP scaffolds(1) seeded with BMSCs;(2) wrapped with osteogenic matrix cell sheets; or(3) both. Constructs were implanted into a femoral defect model in rats and bone growth was evaluated by radiography, histology, biochemistry, and mechanical testing after 8 wk. RESULTS: In bone defects, constructs implanted with cell sheets showed callus formation with segmentalor continuous bone formation at 8 wk, in contrast to TCP seeded with BMSCs, which resulted in bone nonunion. Wrapping TCP constructs with osteogenic matrix cell sheets increased their osteogenic potential and resulting bone formation, compared with conventional bone tissue engineering TCP scaffolds seeded with BMSCs. The compressive stiffness(mean ± SD) values were 225.0 ± 95.7, 30.0 ± 11.5, and 26.3 ± 10.6 MPa for BMSC/TCP/Sheet constructs with continuous bone formation, BMSC/TCP/Sheet constructs with segmental bone formation, and BMSC/TCP constructs, respectively. The compressive stiffness of BMSC/TCP/Sheet constructs with continuous bone formation was significantly higher than those with segmental bone formation and BMSC/TCP constructs.CONCLUSION: This technique is an improvement over current methods, such as TCP substitution, and is useful for hard tissue reconstruction and inducing earlier bone union in defects. | Tomoyuki Ueha Manabu Akahane Takamasa Shimizu Yoshinobu Uchihara Yusuke Morita Naoya Nitta Akira Kido Yusuke Inagaki Kenji Kawate Yasuhito Tanaka | 2015 | World Journal of Stem Cells2015，7，5： | 7 |
| 13 | Classifying genotype F of hepatitis B virus into F1 and F2 subtypes显示文摘AIM: To explore the propriety of providing hepatitis B virus(HBV) genotypes F and H with two distinct genotypes.METHODS: Eleven HBV isolates of genotype F (HBV/F)were recovered from patients living in San Francisco,Japan, Panama, and Venezuela, and their full-length sequences were determined. Phylogenetic analysis was carried out among them along with HBV isolates previously reported.RESULTS: Seven of them clustered with reported HBV/F isolates in the phylogenetic tree constructed on the entire genomic sequence. The remaining four flocked on another branch along with three HBV isolates formerly reported as genotype H. These seven HBV isolates, including the four in this study and the three reported, had a sequence divergence of 7.3-9.5% from the other HBV/F isolates,and differed by > 13.7% from HBV isolates of the other six genotypes (A-E and G). Based on a marked genomic divergence, falling just short of >8% separating the seven genotypes, these seven HBV/F isolates were classified into F2 subtype and the former seven into F1 subtype provisionally. In a pairwise comparison of the S-gene sequences among the 7 HBV/F2 isolates and against 47HBV/F1 isolates as well as 136 representing the other six genotypes (A-E and G), two clusters separated by distinct genetic distances emerged.CONCLUSION: Based on these analyses, classifying HBV/F isolates into two subtypes (F1 and F2) would be more appropriate than providing them with two distinct genotypes (F and H). | Hideaki Kato Kei Fujiwara Robert G. Gish Hiroshi Sakugawa Hiroshi Yoshizawa Fuminaka Sugauchi Etsuro Orito Ryuzo Ueda Yasuhito Tanaka Takanobu Kato Yuzo Miyakawa Masashi Mizokami | 2005 | World Journal of Gastroenterology2005，11，40： | 6 |
| 14 | 固沙林庇护区内降尘特征的初步观测显示文摘采用野外定位实测法,连续两年对科尔沁沙地24龄人工固沙杨树(Populussimonii)林庇护区内4~6月份及强沙尘暴事件中的降尘特征进行了观测研究。结果表明:(1)林地庇护区内4、5月份的降尘量较多,分别为273和437kg/hm2,6月份的降尘量较少,为171kg/hm2。(2)林地中央的滞尘效应在风蚀季节和强沙尘暴天气过程中十分显著。(3)林地庇护区内的降尘中粒径<0.02mm颗粒含量占60.7%,降尘中的全C、全N和速效P含量分别高达1.676%、0.163%和210.66mg/kg,这对风沙土表层土壤的细化和养分的积累具有重要的生态学意义。 | 张华 何红 李锋瑞 Yasuhito Shirato | 2005 | 干旱区地理2005，28，2： | 5 |
| 15 | Independent risk factors and predictive score for the development of hepatocellular carcinoma in chronic hepatitis B显示文摘 | Man-Fung Yuen Yasuhito Tanaka Daniel Yee-Tak Fong James Fung Danny Ka-Ho Wong John Chi-Hang Yuen David Yiu-Kuen But Annie On-On Chan Benjamin Chun-Yu Wong Masashi Mizokami Ching-Lung Lai | 2008 | Journal of Hepatology2008，，1： | 5 |
| 16 | Novel point mutations and mutational complexes in the enhancer II, core promoter and precore regions of hepatitis B virus genotype D1 associated with hepatocellular carcinoma in Saudi Arabia显示文摘 | Anis Khan Mohammed A. Al Balwi Yasuhito Tanaka Ali Hajeer Faisal M. Sanai Ibrahim Al Abdulkarim Latifah Al Ayyar Motasim Badri Dib Saudi Waleed Tamimi Masashi Mizokami Bandar Al Knawy | 2013 | Int J Cancer2013，，12： | 4 |
| 17 | 乙型肝炎病毒基因型C亚型和核心启动子、前C/核心区基因变异的关系显示文摘目的研究慢性乙型肝炎病毒(HBV)感染者中HBV基因型C亚型(HBV/C)的核心启动子、前C/核心区基因变异情况,分析HBV/C亚型的病毒学特征。方法用酶联免疫法(ELISA)筛选出79例HBV/C,再用聚合酶链反应-限制性酶长度多态性分析方法(PCR-RFLP)进行HBV/C亚型分析;同时针对HBV核心启动子、preC/核心区基因进行半巢式PCR及PCR产物直接测序。结果①79例HBV/C中,33例(41·8%)为HBV/C1亚型,46例(58·2%)为HBV/C2亚型。②HBV/C1亚型仅见于来自中国南方的患者(P<0·0001)。③A1898位点变异仅见于HBV/C1亚型(P=0·056),V1753位点变异在HBV/C1亚型中多见(P<0·05);HBV/C2以T1858(90%)、A1896(40%)位点变异多见(P<0·008)。T1762/A1764位点变异在HBV/C两种亚型中均常见。④肝细胞癌(HCC)患者中,V1753和T1762/A1764变异最常见(P<0·05)。结论HBV/C1和HBV/C2在中国有明显的地区差异;V1753合并T1762/A1764双变异与发展为HCC相关,尤其在HBV/C1患者。 | 袁静 龚作炯 Masashi Mizokami Yasuhito Tanaka Fuat Kurbanov Etsum Orito 徐六妹 蒋小玲 赖伟珍 陆坚 周伯平 | 2006 | 中华实验和临床病毒学杂志2006，20，4： | 3 |
| 18 | Distribution of HBV genotypes among HBV carriers in Benin:phylogenetic analysis and virological characteristics of HBV genotype E显示文摘AIM: To determine the distribution of Hepatitis B virus (HBV) genotypes in Benin, and to clarify the virological characteristics of the dominant genotype.METHODS: Among 500 blood donors in Benin, 21 HBsAg-positive donors were enrolled in the study. HBV genotypes were determined by enzyme immunoassay and restriction fragment length polymorphism. Complete genome sequences were determined by PCR and direct sequencing.RESULTS: HBV genotype E (HBV/E) was detected in 20/21 (95.2%), and HBV/A in 1/21 (4.8%). From the age-specific prevalence of HBeAg to anti-HBe seroconversion (SC) in 19 HBV/E subjects, SC was estimated to occur frequently in late teens in HBV/E.The comparison of four complete HBV/E genomes from HBeAg-positive subjects in this study and five HBV/E sequences recruited from the database revealed that HBV/E was distributed throughout West Africa with very low genetic divers ity (nucleotide homology 96.7-99.2%).Based on the sequences in the basic core promoter (BCP)to precore region of the nine HBV/E isolates compared to those of the other genotypes, a nucleotide substitution in the BCP, G1757A, was observed in HBV/E.CONCLUSION: HBV/E is predominant in the Republic of Benin, and SC is estimated to occur in late teens in HBV/E. The specific nucleotide substitution G1757A in BCP, which might influence the virological characteristics,is observed in HBV/E. | Kei Fujiwara Yasuhito Tanaka Etsuro Orito Tomoyoshi Ohno Takanobu Kato Kanji Sugihara Izumi Hasegawa Mayumi Sakurai Kiyoaki Ito Atsushi Ozasa Yuko Sakamoto Isao Arita Ahmed El-Gohary Agossou Benoit Sophie I Ogoundele-Akplogan Namiko Yoshihara Ryuzo Ueda Masashi Mizokami | 2005 | World Journal of Gastroenterology2005，11，41： | 3 |
| 19 | New combination test for hepatitis C virus genotype and viral load determination using Amplicor GT HCV MONITOR test v2.0显示文摘AIM: To develop a new sensitive and inexpensive hepatitis C virus (HCV) combination test (HCV Guideline test) that enables the determination of HCV genotypes 1, 2 and 3,and simultaneous determination of HCV viral load using commercial Amplicor GT HCV MONITOR test v2.0 (microwell version).METHODS: The HCV Guideline test used the PCR product generated in commercial Amplicor GT HCV Monitor test v2.0 for viral load measurement using microwell plate version of Amplicor HCV Monitor and also captured on separate plates containing capture probes and competitive oligonucleotide probes specific for HCV genotypes 1, 2 and 3, The HCV genotype was subsequently determined using the biotin-labeled PCR product and five biotin-labeled HCV-specific probes.RESULTS: The sensitivity of the HCV Guideline test was 0.5 KIU/mL. Specificity of the HCV Guideline test was confirmed by direct sequencing of HCV core region and molecular evolutionary analyses based on a panel of 31 samples. The comparison of the HCV Guideline test and an in-house HCV core genotyping assay using 252 samples from chronic hepatitis C patients indicated concordant results for 97.2% of samples (59.5% genotype 1, 33.7% genotype 2, 6.0% genotype 3, and 0.8% mixed genotypes).Similarly, the HCV Guideline test showed concordance with a serological test, and the serological test failed to assign any serotype in 12.7% of the samples, indicating a better sensitivity of the HCV Guideline test.CONCLUSION: Clinically, both viral load and genotypes (1, 2 and 3) have been found to be major predictors of antiviral therapy outcome regarding chronic hepatitis C based on guidelines and they are, in normal circumstances,performed as separate stand-alone assays. The HCV Guideline test is a useful method for screening large cohorts in a routine clinical setting for determining the treatment regimen and for predicting the outcome of antiviral therapy of chronic hepatitis C. | Motokazu Mukaide Yasuhito Tanaka Hirokazu Kakuda Kei Fujiwara Fuat Kurbanov Eturo Orito Kentaro Yoshioka Kiyotaka Fujise Shoji Harada Takazumi Kozaki Kazuo Takemura Kazumasa Hikiji Masashi Mizokami | 2005 | World Journal of Gastroenterology2005，11，4： | 3 |
| 20 | Effectiveness of clip-and-snare method using pre-looping technique for gastric endoscopic submucosal dissection显示文摘AIM:To evaluate efficacy and safety of clip-and-snare method using pre-looping technique(CSM-PLT)for gastric endoscopic submucosal dissection(ESD).METHODS:In the CSM-PLT method,a clip attached to the lesion side was strangulated with a snare,followed by application of an appropriate tension to the lesion independent of an endoscope.Twenty consecutive lesions were resected by ESD using CSM-PLT(CSM-PLT group)and compared with a control group,including20 lesions that were resected by conventional ESD.The control group was matched based on the size and location of the lesion,presence of pathologic fibrosis,and experience of endoscopists.Total procedure time of ESD,proportion of en bloc resection,and complications were analyzed.RESULTS:The total procedure time for the CSM-PLT group was significantly shorter than that for the control group(38.5 min vs 59.5 min,P=0.023);all lesions were resected en bloc by ESD.There was no significant difference in complications between the two groups.Moreover,there was no complication in the CSM-PLT group.In one large lesion(size:74 mm)that underwentextensive CSM-PLT during ESD,we used an additional CSM-PLT on another edge of the lesion after achieving submucosal resection to the maximum extent possible during initial CSM-PLT.In two lesions,the snare came off the lesion together with the clip after a sudden pull;nevertheless,ESD was successful in all lesions.CONCLUSION:CSM-PLT was an effective and safe method for gastric ESD. | Naohiro Yoshida Hisashi Doyama Ryosuke Ota Yasuhito Takeda Hiroyoshi Nakanishi Kei Tominaga Shigetsugu Tsuji Kenichi Takemura | 2016 | World Journal of Gastrointestinal Endoscopy2016，8，12： | 3 |
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