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| 1 | Remifentanil mimics cardioprotective effect of ischemic preconditioning via protein kinase C activation in open chest of rats显示文摘Aim: To examine whether the protective effect of remifentanil preconditioning (RPC) on postischemic hearts is mediated by protein kinase (PKC) activation in comparison with ischemic preconditioning (IPC). Methods: Male Sprague-Dawley rats were anesthetized and their chests were opened. The experiment was performed with chelerythrine (CHE, 2mg/kg), GF109203X (0.05mg/kg) protein kinase C (PKC) inhibitors administered before RPC (remifentanil 6μg·kg^-1·min^-1×3 cycle) or IPC, respectively. Infarct size (IS), as a percentage of the area at risk (AAR), was determined by triphenyltetrazolium staining. Results: In groups subjected to IPC and RPC the IS/AAR were significantly reduced (IS/AAR from 52.7%±5.5% to 12.9%±3.4%, P<0.01 vs CON and 16.2%±6.4%, P<0.01 vs CON), respectively. CHE and GF, both PKC inhibitors, administered 5min before RPC or IPC completely abolished the cardioprotective effect of RPC (IS/AAR: CHE+RPC 51.2%±5.0%,GF+RPC 53.6%±6.1%, P>0.05 vs CON) or IPC (CHE+IPC 53.7%±4.3%, GF+IPC 54.1%±6.2%, P>0.05 vs CON). The difference was not significant in any of the hemodynamic parameters between control and treatment groups during ischemia and reperfusion. Conclusion: Remifentanil confers myocardial protection against ischemic injury through a mechanism that is similar to IPC and involves PKC activation. | YeZHANG Zhi-wuCHEN MichaelGIRWIN Tak-mingWONG | 2005 | Acta Pharmacologica Sinica2005，26，5： | 32 |
| 2 | Development of Gossypium barbadense chromosome segment substitution lines in the genetic standard line TM-1 of Gossypium hirsutum显示文摘Chromosome segment substitution lines (CSSL) consist of a battery of nearisogenic lines that have been developed and cover the entire genome of some crops. With the exception of one homozygous chromosome segment transferred from a donor parent, the remaining genome of each CSSL line is the same as the recipient parent. It is an ideal material for genome research and particularly QTL mapping. In the present study, we first developed one set of CSSL lines using G. hirsutum acc. TM-1 (the genetic standard), as the recipient parent and G. barbadense cv. Hai7124 as the donor parent using molecular assisted-selection in BC5S1-3 generations. The CSSL consisted of 330 different lines, in which 1-4 different lines had the same or overlapping substituted segments. The genetic length of the substituted segments covered 5271.9 cM with an average segment distance of 10.9 cM, 1.5 times the total genetic length of Upland cotton (3514.6 cM). The substituted segments of each line varied in length, ranging from 3.5 cM for the shortest segment to 23.2 cM in the longest segment. Our CSSL have not yet to cover the entire tetraploid cotton genome, due to the absence of some donor parent interval segments. | WANG Peng DING YeZhang LU QiongXian GUOWangZhen ZHANG TianZhen | 2008 | Chinese Science Bulletin2008，53，10： | 14 |
| 3 | Fine mapping of the dominant glandless Gene Gl_2~e in Sea-island cotton (Gossypium barbadense L.)显示文摘Gle2 is a mutant gene that controls glandless trait in cotton plants and seeds. It is an important gene resource to gossypol-free cottonseed breeding. The objective of this research was to develop SSR markers tightly linked with Gle2 by using the F2 segregating population containing 1599 plants derived from the cross of G. hirsutum genetic standard line TM-1 and G. barbadense glandless mutant line Hai-1. Genetic analysis suggested that the Gle2 was an incomplete dominant gene. Based on the backbone of genetic linkage map from G. hirsutum × G. barbadense BC1 published by our laboratory,Gle2 was lo-cated between CIR362 and NAU2251b,NAU3860b,STV033,with a genetic distance 9.27 and 0.96 cM,respectively. This result is useful for cloning Gle2 gene by map-based cloning method. | DONG ChengGuang DING YeZhang GUO WangZhen ZHANG TianZhen | 2007 | Chinese Science Bulletin2007，52，22： | 12 |
| 4 | Structure analysis and expressions of a novel tetratransmembrane protein,lysosoma-associated protein transmembrane 4β associated with hepatocellular carcinoma显示文摘AIM: To analyze the structure and expressions of the protein encoded by an HCC-associated novel gene, lysosomeassociated protein transmembrane 4 β (LAPTM4B). METHODS: Primary structure and fundamental characteristics of LAPTM4B protein were analysed with bioinformatics. Expressions of LAPTM4B in HCC tissues and various cell lines were detected using polyclonal antibodies and Western blot. RESULTS: LAPTM4B encoded two isoforms of proteins with molecular masses 35-ku and 24-ku, respectively. The expression level of LAPTM4B-35 protein in HCC tissues was dramatically upregulated and related to the differentiation status of HCC tissues, and it was also high in some cancer cell lines. Computer analysis showed LAPTM4B was an integral membrane protein with four transmembrane domains. LAPTM4B showed relatively high homology to LAPTM4A and LAPTM5 in various species. CONCLUSION: LAPTM4B gene encoded two isoforms of tetratansmembrane proteins, LAPTM4B-35 and LAPTM4B-24. The expression of LAPTM4B-35 protein is upregulated and associated with poor differentiation in human HCC tissues, and also at high levels in some cancer cell lines. LAPTM4B is an original and conserved protein. | Xin-RongLiu Rou-LiZhou Qing-YunZhang YeZhang Yue-YingJin MingLin Jing-AnRui Da-XiongYe | 2004 | World Journal of Gastroenterology2004，10，11： | 10 |
| 5 | QTL mapping in A-genome diploid Asiatic cotton and their congruence analysis with AD-genome tetraploid cotton in genus Gossypium显示文摘Asiatic cotton(Gossypium arboreum L.) is an Old World cultivated cotton species.The sinense race was planted extensively in China.Due to the advances in spinning technology during the last century,the species was replaced by the New World allotetraploid cotton G.hirsutum L.Gossypium arboreum is still grown in India and Pakistan and also used as an elite in current cotton breeding programs.In addition,G.arboreum serves as a model for genomic research in Gossypium.In the present study,we generated an A-genome diploid cotton intraspecific genetic map including 264 SSR loci with three morphological markers mapped to 13 linkage groups.The map spans 2,508.71 cM with an average distance of 9.4 cM between adjacent loci.A population containing 176 F2:3 families was used to perform quantitative trait loci(QTL) mapping for 17 phenotypes using Multiple QTL Model(MQM) of MapQTL ver 5.0.Overall,108 QTLs were detected on 13 chromosomes.Thirty-one QTLs for yield and its components were detected in the F2 population.Forty-one QTLs for yield and its components were detected in the F2:3 families with a total of 43 QTLs for fiber qualities.Two QTLs for seed cotton weight/plant and lint index and three QTLs for seed index were consistently detected both in F2 and F2:3.Most QTLs for fiber qualities and yields were located at the same interval or neighboring intervals.These results indicated that the negative correlation between fiber qualities and yield traits may result from either pleiotropic effect of one gene or linkage effects of multiple closely linked genes. | Xuexia Ma Yezhang Ding Baoliang Zhou Wangzhen Guo Yanhui Lv Xiefei Zhu Tianzhen Zhang | 2008 | Journal of Genetics and Genomics2008，35，12： | 5 |
| 6 | Partial Abstracts of East China Symposium of Biochemistry & Molecular Biology-2003 (Part Ⅱ)——Signal Transduction Pathway of the Protective Effects of Ginkgolides on Chemical Hypoxia Injury to CorticalNeurons显示文摘Ginkgolides (Gin), mainly including A, B, C, J, M and belonging to terpene lactone are one of the main active constituents in the extractof leaves of Ginkgo biloba L. Gin B is a specific and potent antagonist of platelet-activating factor (PAF). Some animal and clinical data showthe potential anti-ischemic/hypoxic effects and obvious protective effects of Gin on neurons, however the mechanisms of which and | LiZHU YeZHANG Hong-ShanCHEN Shu-YiJIN | 2004 | Acta Biochimica et Biophysica Sinica2004，36，2： | 1 |
| 7 | Circulating Toll‐like receptor (TLR) 2, TLR4, and regulatory T cells in patients with chronic hepatitis C显示文摘 | JIU‐PINGWANG YEZHANG XINWEI JUNLI XUE‐PINGNAN HAI‐TAOYU YULI PING‐ZHONGWANG XUE‐FANBAI | 2010 | APMIS2010，，4： | 1 |
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