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| 1 | Sirt1-ROS-TRAF6 Signaling-Induced Pyroptosis Contributes to Early Injury in Ischemic Mice显示文摘Stroke is an acute cerebro-vascular disease with high incidence and poor prognosis,most commonly ischemic in nature.In recent years,increasing attention has been paid to inflammatory reactions as symptoms of a stroke.However,the role of inflammation in stroke and its underlying mechanisms require exploration.In this study,we evaluated the inflammatory reactions induced by acute ischemia and found that pyroptosis occurred after acute ischemia both in vivo and in vitro,as determined by interleukin-1β,apoptosis-associated speck-like protein,and caspase-1.The early inflammation resulted in irreversible ischemic injury,indicating that it deserves thorough investigation.Meanwhile,acute ischemia decreased the Sirtuin 1(Sirtl)protein levels,and increased the TRAF6(TNF receptor associated factor 6)protein and reactive oxygen species(ROS)levels.In further exploration,both Sirtl suppression and TRAF6 activation were found to contribute to this pyroptosis.Reduced Sirtl levels were responsible for the production of ROS and increased TRAF6 protein levels after ischemic exposure.Moreover,N-acetyl-L-cysteine,an ROS scavenger,suppressed the TRAF6 accumulation induced by oxygen-glucose deprivation via suppression of ROS bursts.These phenomena indicate that Sirtl is upstream of ROS,and ROS bursts result in increased TRAF6 levels.Further,the activation of Sirtl during the period of ischemia reduced ischemiainduced injury after 72 h of reperfusion in mice with middle cerebral artery occlusion.In sum,these results indicate that pyroptosis-dependent machinery contributes to the neural injury during acute ischemia via the Sirt1-ROS-TRAF6 signaling pathway.We propose that inflammatory reactions occur soon after oxidative stress and are detrimental to neuronal survival;this provides a promising therapeutic target against ischemic injuries such as a stroke. | Weijie Yan Wei Sun Jiahui Fan Haiqing Wang Song Han Junfa Li Yanling Yin | 2020 | Neuroscience Bulletin2020，36，8： | 9 |
| 2 | Elevated 8-oxo-7,8-dihydro-2'-deoxyguanosine in genome of T24 bladder cancer cells induced by halobenzoquinones显示文摘Halobenzoquinones(HBQs) are an emerging class of halogenated disinfection byproducts(DBPs) in drinking water, which raised public concerns due to potential carcinogenic effects to human bladder. Our previous work demonstrated that HBQs and hydrogen peroxide(H_2O_2)together generated oxidative DNA damage via a metal-independent and intercalationenhanced oxidation mechanism in vitro. This study further investigated the efficiency of various HBQs to induce oxidative DNA damage in T24 bladder cancer cells. Compared with T24 cells without treatment(3.1 lesions per 10~6 d G), the level of 8-oxo-7,8-dihydro-2′-deoxyguanosine(8-oxod G) significantly increased by 1.4, 3.2, 8.8, and 9.2 times after treatment with tetrabromo-1,4-benzoquinone(TBBQ), terachloro-1,4-benzoquinone(TCBQ),2,6-dichloro-1,4-benzoquinone(2,6-DCBQ) and 2,5-dichloro-1,4-benzoquinone(2,5-DCBQ) for24 hr, respectively. Interestingly, we found that the oxidative potency of HBQs in T24 cells(2,5-DCBQ ≈ 2,6-DCBQ > TCBQ > TBBQ) is inconsistent with that of in vitro ds DNA oxidation(TCBQ > TBBQ > 2,5-DCBQ > 2,6-DCBQ), suggesting HBQs induce oxidative lesions in cellular genomic DNA probably involved with a complex mechanism. | Tian Xu Junfa Yin Shaokun Chen Dapeng Zhang Hailin Wang | 2018 | Journal of Environmental Sciences2018，30，1： | 5 |
| 3 | Rapid and efficient chiral separation of nateglinide and its l-enantiomer on monolithic molecularly imprinted polymers显示文摘 | Yin Junfa Yang Gengliang Chen Yi | 2005 | Journal of Chromatography A2005，，1090： | 1 |
| 4 | Rapid and efficient chiral separation of nateglinide and its l-enantiomer on monolithic molecularly imprinted polymers显示文摘 | YIN Junfa YANG Gengliang CHEN Yi | 2005 | J Chromatogr A2005，1090，： | 1 |
| 5 | Thermal transient response of GaAs FETs under intentional electromagnetic interference (IE- MI)显示文摘 | Xu Jianfeng Yin Wenyan Mao Junfa | 2008 | IEEE Trans on Electromagn Compat2008，50，2： | 1 |
| 6 | Size-dependent toxicity of nano-C60 aggregates : more sen- sitive indication by apoptosis-related Bax translocation in cultured human cells显示文摘 | SONG Maoyong YUAN Shaopeng YIN Junfa | 2012 | Environ Sci Technol2012，46，6： | 1 |
| 7 | Latest progress in the research of protein kinase C(PKC)isoform-specific signaling and PKC-modulated autophagy in ischemic stroke显示文摘Ischemic stroke is a major cause of morbidity and mortality,and currently there is no effective treatment.The family of protein kinase C(PKCs)could phosphorylate serine or threonine residues of its substrate proteins and play a key role in the ischemia/reperfusion injury.Autophagy is essential for maintaining cell homeostasis under physiological condition and acts as a double-edged sword in the process of ischemic neuronal death.In this article,we reviewed the PKCs isoform-specific signaling pathways and PKC-modulated autophagy in ischemic stroke. | Rongrong Hua Nan Zhang Yanling Yin Junfa Li | 2017 | Journal of Translational Neuroscience2017，2，2： | 1 |
| 8 | Rapid and efficient chiral separation of nateglinide and its Ⅰ-enantiomer on mo- nolithic molecularly imprinted polymers 显示文摘 | Yin Junfa Yang Gengliang Chen Yi | 2005 | J Chromatogy A2005，1090，2： | 1 |
| 9 | Purification and determination of stachyose in Chinese artichoke ( Stachys Sieboldii Miq.) by high-performance liquid chromatography with evaporative light scattering detection显示文摘 | Junfa Yin Gengliang Yang Sumin Wang Yi Chen | 2006 | Talanta2006，，1： | 1 |
| 10 | Improved preparation and identification of aristolochic acid-DNA adducts by solid-phase extraction with liquid chromatography-tandem mass spectrometry显示文摘Aristolochic acid (AA) is a known nephrotoxin and potential carcinogen, which can form covalent DNA adducts after metabolic activation in vivo and in vitro. A simple method for preparation and characterization of aristolochic acid-DNA adducts was developed. Four AA-adducts were synthesized by a direct reaction of AAI/AAII with 2'-deoxynucleosides. The reaction mixture was first cleaned-up and pre-concentrated using solid phase extraction (SPE), and further purified by a reversed-phase high performance liquid chromatography (HPLC). By the application of developed SPE procedure, matrices and byproducts in reaction mixture could be greatly reduced and adducts of high purity (more than 94% as indicated by HPLC) were obtained. The purified AA-DNA adducts were identified and characterized with liquid-electrospray ionization-quadrupole-time of flight-mass spectrometry (LC-ESI-Q-TOF-MS/MS) and LC-Diode array detector-fluorescence (LC-DAD-FL) analysis. This work provides a robust tool for possible large-scale preparation of AA-DNA adduct standards, which can promote the further studies on carcinogenic and mutagenic mechanism of aristolochic acids. | GAO Haiyan GUO Fangqiu FENG Feng YIN Junfa SONG Maoyong WANG Hailin | 2009 | Journal of Environmental Sciences2009，21，12： | 0 |
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