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| 1 | Molecular diagnostics in a teacup:Non-Instrumented Nucleic Acid Amplification(NINA) for rapid,low cost detection of Salmonella enterica显示文摘We report on the use of a novel non-instrumented platform to enable a Loop Mediated isothermal Amplification(LAMP) based assay for Salmonella enterica.Heat energy is provided by addition of a small amount(<150 g) of boiling water,and the reaction temperature is regulated by storing latent energy at the melting temperature of a lipid-based engineered phase change material.Endpoint classification of the reaction is achieved without opening the reaction tube by observing the fluorescence of sequence-specific FRET-based assimilating probes with a simple handheld fluorometer.At or above 22℃ ambient temperature the non-instrumented devices could maintain reactions above a threshold temperature of 61℃ for over 90 min-significantly longer than the 60 min reaction time.Using the simple format,detection limits were less than 20 genome copies for reactions run at ambient temperatures ranging from 8 to 36℃.When used with a pre-enrichment step and non-instrumented DNA extraction device,trace contaminations of Salmonella in milk close to 1 CFU/mL could be reliably detected.These findings illustrate that the non-instrumented amplification approach is a simple,viable,low-cost alternative for field-based food and agricultural diagnostics or clinical applications in developing countries. | KUBOTA Ryo LABARRE Paul WEIGL Bernhard H LI Yong HAYDOCK Paul JENKINS Daniel M | 2013 | Chinese Science Bulletin2013，58，10： | 4 |
| 2 | A NEW FLAVONE C-GLYCOSIDE FROM SCUTELLARIA BAICALENSIS显示文摘ANEWFLAVONEC-GLYCOSIDEFROMSCUTELLARIABAICALENSIS￥YongYuZHANG;XianLI;YunZhenGOU(ShenyangPharmaceuticalUniversity,Shenyang11001... | Yong Yu ZHANG Xian LI Yun Zhen GOU(Shenyang Pharmaceutical University, Shenyang 110015)Yoshihiro HARIGAYA, Masayuki ONDASchool of Pharmaceutical Sciences, Kitasato University, Tokyo 108 ,JapanKadsunort H^SHIMO’rO, yukinobu iK EYA, Minoru OKAD^, cacao M^ | 1994 | Chinese Chemical Letters1994，5，10： | 3 |
| 3 | Translocation of a calcium-permeable cation channel induced by insulin-like growth factor-1 显示文摘 | Kanzaki M Yong Q Z Mashima H | 1999 | Nature Cell Biology1999，1，3： | 1 |
| 4 | Time-dependent channel formation in a laser-produced plasma 显示文摘 | YONG P E FOORD M E HAMMER J H | 1995 | Phys Rev Lett1995，76，6： | 1 |
| 5 | Formulations can affect rhizosphere colonization and biocontrol efficiency of Trichoderma harzianum SQR-T037 against Fusarium wilt of cucumbers显示文摘 | Yang X M Chen L H Yong X Y | 2011 | Biology and Fertility of Soils2011，47，3： | 1 |
| 6 | Identification of a rice APETALA3 homologue by yeast two-hybrid screening显示文摘 | YONG H M JI Y J HONG G K | 1999 | Plant Molecular Biology1999，40，： | 1 |
| 7 | An experimental investigation of the failure mechanism of simulated transverselyisotropic rocks显示文摘 | Yong M T Ming C K Chamg H J | 2006 | International Journal of Rock Mechanics and Mining Science2006，43，： | 1 |
| 8 | Catheter ablation of accessory pathways,atrioventricular nodal reentrant tachycardia, and the atrioventricular junction:final results of a prospective, multicenter clinical trial 显示文摘 | Calkins H Yong P Miller J M | 1999 | Circulation1999，99，2： | 1 |
| 9 | Oral delivery of peptide drugs using nanoparticles self-assembled by poly-y -glutamic acid and a ehitosan derivative functionalized by trimethylation 显示文摘 | FWU L M YONG Y W YU H L | 2008 | Bioeonjugate Chem2008，19，6： | 1 |
| 10 | Transactivation of microRNA - 383 by steroidogenic factor - 1 promotes estradiol release from mouse ovarian granjaLosa cells by targeting RBMS1 显示文摘 | Yin M M 1 0 M R Yao G 1) Tian H Lian J Liu L Liang M Wang Yong and Fei Sun | 2012 | Molecular Endocrinologie2012，26，7： | 1 |
| 11 | Damage identification of structures with uncertain frequency and mode shape data 显示文摘 | Xia Yong Hao H Brownjohn J M W | 2002 | Earthquake Engineering and Structural Dynamics2002，31，： | 1 |
| 12 | Delay-dependent H control of linear discrete-time systems with an interval-like time-varying delay显示文摘 | Yong H E WU M HAN Q L | 2008 | Int J Syst Sci2008，39，4： | 1 |
| 13 | A new isorhamnetin glycoside and other phenolic compounds from Callianthemum taipaicum 显示文摘 | Dong M W Wen J P Yong H W | 2012 | Molecules2012，17，10： | 1 |
| 14 | Capacity fading of spinel phase LiMn2O4 with cycling 显示文摘 | Yong M Y Ahn D S Park H R | 1999 | Journal Power Sources1999，83，： | 1 |
| 15 | Performance eval- uation of cryogenically treated tungsten carbide tools in tum- ing显示文摘 | Yong A Y L Seah K H W Rahman M | 2006 | International Journal of Machine Tools & Manufac- ture2006，46，： | 1 |
| 16 | Development and Psychometric Evaluation of the Resilience Scale显示文摘 | Wagnild G M Yong H M | 1993 | J Nurs Meas1993，1，2： | 1 |
| 17 | Development and psychomet- ric evaluation of the resilience scale显示文摘 | Wagnild G M Yong H M | 1993 | J Nurs Measure1993，1，2： | 1 |
| 18 | A human endothelial cellfeeder system that efficiently supports the undifferentiated growth ofmouse embryonic stem cells 显示文摘 | Zhou H S Yong J Sun X M | 2008 | Differentiation2008，76，9： | 1 |
| 19 | Formation and diagenesis of weathering profiles 显示文摘 | Nesbitt H W Yong G M | 1989 | Metamorph Geol1989，97，： | 1 |
| 20 | Interspecies variation of Kitasatospora recifensis endophytic from yam bean produ-cing thermostable amylases in alternative media显示文摘 | Yong H P Jin W B Janete M A | 2007 | World Journal of Microbiology and Biotechnology2007，23，12： | 1 |
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