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| 1 | Cryo-self-assembled silk fibroin sponge as a biodegradable platform for enzyme-responsive delivery of exosomes显示文摘Although advances in protein assembly preparation have provided a new platform for drug delivery during tissue engineering,achieving long-term controlled exosome delivery remains a significant challenge.Diffusion-dominated exosome release using protein hydrogels results in burst release of exosomes.Here,a fibroin-based cryo-sponge was developed to provide controlled exosome release.Fibroin chains can self-assemble into silk I structures under ice-cold conditions when annealed above the glass transition temperature.Exosome release is enzyme-responsive,with rates primarily determined by enzymatic degradation of the scaffolds.In vivo experiments have demonstrated that exosomes remain in undigested sponge material for two months,superior to their retention in fibrin glue,a commonly used biomaterial in clinical practice.Fibroin cryo-sponges were implanted subcutaneously in nude mice.The exosome-containing sponge group exhibited better neovascularization and tissue ingrowth effects,demonstrating the efficacy of this exosome-encapsulating strategy by realizing sustained release and maintaining exosome bioactivity.These silk fibroin cryo-sponges containing exosomes provide a new platform for future studies of exosome therapy. | Muyang Sun Qi Li Huilei Yu Jin Cheng Nier Wu Weili Shi Fengyuan Zhao Zhenxing Shao Qingyang Meng Haifeng Chen Xiaoqing Hu Yingfang Ao | 2022 | Bioactive Materials2022，7，2： | 4 |
| 2 | A shear-thinning,ROS-scavenging hydrogel combined with dental pulp stem cells promotes spinal cord repair by inhibiting ferroptosis显示文摘Spinal cord injury(SCI)is a serious clinical disease.Due to the deformability and fragility of the spinal cord,overly rigid hydrogels cannot be used to treat SCI.Hence,we used TPA and Laponite to develop a hydrogel with shear-thinning ability.This hydrogel exhibits good deformation,allowing it to match the physical properties of the spinal cord;additionally,this hydrogel scavenges ROS well,allowing it to inhibit the lipid peroxidation caused by ferroptosis.According to the in vivo studies,the TPA@Laponite hydrogel could synergistically inhibit ferroptosis by improving vascular function and regulating iron metabolism.In addition,dental pulp stem cells(DPSCs)were introduced into the TPA@Laponite hydrogel to regulate the ratios of excitatory and inhibitory synapses.It was shown that this combination biomaterial effectively reduced muscle spasms and promoted recovery from SCI. | Yibo Ying Zhiyang Huang Yurong Tu Qiuji Wu Zhaoyu Li Yifan Zhang Huilei Yu Annian Zeng Hanzhi Huang Jiahui Ye Weiyang Ying Min Chen Zhiyi Feng Ziyue Xiang Qingsong Ye Sipin Zhu Zhouguang Wang | 2023 | Bioactive Materials2023，，4： | 2 |
| 3 | Astrocyte elevated gene-1: a novel independent prognostic biomarker for metastatic ovarian tumors显示文摘 | Cong Li Kexin Chen Jianping Cai Qing-Tao Shi Yinghong Li Lejing Li Hongtao Song Huilei Qiu Yu Qin Jing-Shu Geng | 2014 | Tumor Biology2014，，4： | 1 |
| 4 | Enzymatic Production of L-menthol by a high substrate concentration tolerable esterase from newly isolated Bacillus subtilis ECU0554显示文摘 | Zheng Gaowei Yu Huilei Zhang Jiandong | 2009 | Adv Synth Catal2009，351，： | 1 |
| 5 | ImpI'oved o- chlorobenzoylformate bioreduction by stabilizing aldo-keto reductase YtbE with additives显示文摘 | XU Yanpeng GUAN Yuehugh YU Huilei | 2014 | Journal of molecular catalysis B: enzymatic2014，104，2： | 1 |
| 6 | Immobilization of trophic anaerobic acetogen for semi-continuous syngas fermentation显示文摘The massive consumption of fossil energy force s people to find new source s of energy.Syngas fermentation has become a hot research field as its high potential in renewable energy production and sustainable development.In this study,trophic anaerobic acetogen Morella thermoacetica was successfully immobilized by calcium alginate embedding method.The ability of the immobilized cells on production of acetic acid through syngas fermentation was compared in both airlift and bubble column bioreactors.The bubble column bioreactor was selected as the better type of bioreactor.The production of acetic acid reached 32.3 g·L^(-1) in bubble column bioreactor with a space-time yield of 2.13 g·L^(-1)·d^(-1).The immobilized acetogen could be efficiently reused without significant lag period,even if exposed to air for a short time.A semi-continuous syngas fermentation was performed using immobilized cells,with an average space-time acetic acid yield of 3.20 g·L^(-1)·d^(-1).After 30 days of fermentation,no significant decrea se of the acetic acid production rate was observed. | Lijuan Zhang Peng Hu Jiang Pan Huilei Yu Jianhe Xu | 2021 | Chinese Journal of Chemical Engineering2021，34，1： | 1 |
| 7 | Enzymatic production of 1-menthol by a high substrate concentration tolerable esterase from newly isolated Bacillus subtilis ECU0554 显示文摘 | Zheng Gaowei Yu Huilei Zhang Jiandong | 2009 | Adv Syn Catal2009，351，： | 1 |
| 8 | Rare tumors: a blue ocean of investigation显示文摘Advances in novel drugs,therapies,and genetic techniques have revolutionized the diagnosis and treatment of cancers,substantially improving cancer patients’prognosis.Although rare tumors account for a non-negligible number,the practice of precision medicine and development of novel therapies are largely hampered by many obstacles.Their low incidence and drastic regional disparities result in the difficulty of informative evidence-based diagnosis and subtyping.Sample exhaustion due to difficulty in diagnosis also leads to a lack of recommended therapeutic strategies in clinical guidelines,insufficient biomarkers for prognosis/efficacy,and inability to identify potential novel therapies in clinical trials.Herein,by reviewing the epidemiological data of Chinese solid tumors and publications defining rare tumors in other areas,we proposed a definition of rare tumor in China,including 515 tumor types with incidences of less than 2.5/100000 per year.We also summarized the current diagnosis process,treatment recommendations,and global developmental progress of targeted drugs and immunotherapy agents on the status quo.Lastly,we pinpointed the current recommendation chance for patients with rare tumors to be involved in a clinical trial by NCCN.With this informative report,we aimed to raise awareness on the importance of rare tumor investigations and guarantee a bright future for rare tumor patients. | Shuhang Wang Peiwen Ma Ning Jiang Yale Jiang Yue Yu Yuan Fang Huilei Miao Huiyao Huang Qiyu Tang Dandan Cui Hong Fang Huishan Zhang Qi Fan Yuning Wang Gang Liu Zicheng Yu Qi Lei Ning Li | 2023 | Frontiers of Medicine2023，17，2： | 0 |
| 9 | Facile Access to Chiral Alcohols with Pharmaceutical Relevance Using a Ketoreductase Newly Mined from Pichia guilliermondi显示文摘与另外的功能的组一起的 Chiral 第二等的白酒经常为药品作为重要、珍贵的 synthons 被要求,农业并且另外的好化学药品。与环境地良性的反应条件,宽广反应范围,和高 stereoselectivity 的优点, prochiral 酉同类的 biocatalytic 减小在光学地活跃的白酒的合成提供重要潜力。从 Pichia guilliermondii NRRL Y-324 的 CmCR 相应羰基 reductase 是成功地 overexpressed。底层侧面描述揭示了它的宽广底层特性,盖住芳基酉同类,脂肪族的酉同类和 ketoesters。而且,许多酉同类底层被净化的酶不均匀地减少与一另外 NADPH 新生系统。减小系统展出了优秀 enantioselectivity (>99% ee ) 在所有芳香的酉同类和 ketoesters 的减小,除了 2-bromoacetophenone (93.5% ee ) 。六个酉同类的半准备的减小与高 enantioselectivity 被完成(>99% ee ) 并且隔离收益(>80%) 在 12 h 以内。这研究在 chiral 白酒 enantiomers 的不对称的合成为这酶的进一步的申请提供有用指导。 | Guochao Xu Huilei Yu Jianhe Xu | 2013 | Chinese Journal of Chemistry2013，31，3： | 0 |
| 10 | Preface tovirtual special issue of biocatalysis显示文摘Catalysis plays an important role in human civilization and economic development.Every breakthrough related to the field of catalysis has great impact on our everyday lives.To achieve harmonious development between human and nature through developing chemical processes with environmental friendliness and atom economy,the improvement and innovation of catalytic systems have drawn great attention.Biocatalysis is the engine of life from the very beginning,which is the example that we always learn from,mimic,and aim to surpass in scientific research and industrial development.The goal of the development of biocatalysis technology is tomake our industrial manufacturing go undermild conditions as nature does.However,the current situation of biocatalysis application is still far from the ultimate aim,providing countless opportunities for further development. | Jun Ge Huilei Yu | 2021 | Chinese Journal of Chemical Engineering2021，34，1： | 0 |
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