| 1 | Toxoplasma gondii infection induces cell apoptosis via multiple pathways revealed by transcriptome analysis显示文摘Toxoplasma gondii is a worldwide parasite that can infect almost all kinds of mammals and cause fatal toxoplasmosis in immunocompromised patients.Apoptosis is one of the principal strategies of host cells to clear pathogens and maintain organismal homeostasis,but the mechanism of cell apoptosis induced by T.gondii remains obscure.To explore the apoptosis influenced by T.gondii,Vero cells infected or uninfected with the parasite were subjected to apoptosis detection and subsequent dual RNA sequencing(RNA-seq).Using high-throughput Illumina sequencing and bioinformatics analysis,we found that pro-apoptosis genes such as DNA damage-inducible transcript 3(DDIT3),growth arrest and DNA damage-inducibleα(GADD45 A),caspase-3(CASP3),and high-temperature requirement protease A2(Htr A2)were upregulated,and anti-apoptosis genes such as poly(adenosine diphosphate(ADP)-ribose)polymerase family member 3(PARP3),B-cell lymphoma 2(Bcl-2),and baculoviral inhibitor of apoptosis protein(IAP)repeat containing 5(BIRC5)were downregulated.Besides,tumor necrosis factor(TNF)receptor-associated factor 1(TRAF1),TRAF2,TNF receptor superfamily member 10 b(TNFRSF10 b),disabled homolog2(DAB2)-interacting protein(DAB2 IP),and inositol 1,4,5-trisphosphate receptor type 3(ITPR3)were enriched in the upstream of TNF,TNF-related apoptosis-inducing ligand(TRAIL),and endoplasmic reticulum(ER)stress pathways,and TRAIL-receptor2(TRAIL-R2)was regarded as an important membrane receptor influenced by T.gondii that had not been previously considered.In conclusion,the T.gondii RH strain could promote and mediate apoptosis through multiple pathways mentioned above in Vero cells.Our findings improve the understanding of the T.gondii infection process through providing new insights into the related cellular apoptosis mechanisms. | Kaige DU Fei LU Chengzuo XIE Haojie DING Yu SHEN Yafan GAO Shaohong LU Xunhui ZHUO | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，4： | 1 |
| 2 | Experimental study of the effects of Ulinastatin on reducing MDA in free flaps显示文摘Free flap transplantation is the most important plastic surgery treatment.Although free flap is an important tissue repair tool for plastic surgery,it is easy to happen due to the anastomotic stenosis or thrombosis,as well as the inflammatory reaction caused by ischemia-reperfusion injury of the flap and the inflammation caused by these inflammatory reactions.Microcirculation contracture or occlusion will lead to partial or complete flap necrosis,thus affecting the repair effect.Numerous studies show that neutrophils are closely involved in the process of ischemic injury of free flaps.The purpose of this experiment is to investigate the use of ulinastatin to inhibit the inflammatory infiltration of free flaps to improve the survival of the flap. | Wei Lu Ruixia Qi Yifan Sun Yafan Sun Mingjuan Gu Xiang Gao Peng Yu Jing Sun | 2016 | Discussion of Clinical Cases2016，3，3： | 0 |
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