| 2 | Dietary n-3/n-6 polyunsaturated fatty acid ratio modulates growth performance in spotted seabass(Lateolabrax maculatus)through regulating lipid metabolism,hepatic antioxidant capacity and intestinal health显示文摘An 8-week feeding experiment was carried out to explore the effects of dietary n-3/n-6 polyunsaturated fatty acid(PUFA)ratio on growth performance,lipid metabolism,hepatic antioxidant status,and gut flora of spotted seabass(Lateolabrax maculatus).Six experimental diets were formulated to contain different levels of two purified oil sources including docosahexaenoic and eicosapentaenoic acids enriched oil(n-3)and linoleic acid-enriched oil(n-6)leading to n-3/n-6 PUFA ratios of 0.04,0.35,0.66,1.35,2.45 and16.17.Each diet was fed to triplicate groups of juvenile L.maculatus(11.06±0.20 g,30 fish/tank).Final body weight(FBW),weight gain(WG),specific growth rates(SGR),protein efficiency ratio(PER)and feed utilization efficiency increased as n-3/n-6 PUFA ratio increased up to a certain level,and then decreased thereafter.Fish fed the diet with n-3/n-6 PUFA ratio of 0.66 exhibited the highest FBW,WG,SGR and PER and the lowest feed conversion ratio.Lower n-3/n-6 PUFA ratios induced up-regulated expression of lipid synthesis-related genes(fas,acc2 and srebp-1c)and down-regulated expression of lipolysis related genes(atgl,ppara,cpt-1 and aox).Higher expression of lipolysis-related genes(atgl,ppara and cpt-1)was recorded at moderate n-3/n-6 PUFA ratios(0.66 to 1.35).Moreover,inappropriate n-3/n-6 PUFA ratios triggered up-regulation of pro-inflammatory genes(il-6 and tnf-a)and down-regulation of antiinflammatory genes(il-4 and il-10)in the intestine.The diet with n-3/n-6 PUFA ratio of 0.66 inhibited intestine inflammation,improved intestinal flora richness,increased the abundance of beneficial bacteria such as Lactobacillus,Alloprevotella and Ruminococcus,and reduced the abundance of harmful bacteria including Escherichia-Shigella and Enterococcus.In summary,it could be suggested that a dietary n-3/n-6PUFA ratio of 0.66 can improve growth performance and feed utilization in L.maculatus,as is deemed to be mediated through regulation of lipid metabolism and intestinal flora. | Yanzou Dong Yu Wei Ling Wang Kai Song Chunxiao Zhang Kangle Lu Samad Rahimnejad | 2023 | Animal Nutrition2023，，3： | 0 |
| 3 | 张雷:喀喇昆仑上的守望者显示文摘帕米尔高原是由昆仑山、喀喇昆仑山、兴都库什山和天山山脉交会而成的巨大山结,这里冰峰林立、沟壑纵横、高寒缺氧,是古丝绸之路上的“死亡雪海”,被当代生物学家定义为“生命禁区”。然而,在这片贫瘠荒芜的雪域高原上,却有着世界上平均海拔最高的国门口岸,它以5100米的海拔屹立于冰雪之中,一代代红其拉甫戍边人在这片热土上,与寒星冷月为伍,与雪山冰峰为伴,演绎着无数感人至深的故事。 | 业思聪 Zhang Yanzou | 2022 | 现代世界警察2022，，8： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员