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您的检索式：作者名="Yang Guidi"
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| 1 | Arsenic methylation by an arsenite S-adenosylmethionine methyltransferase from Spirulina platensis显示文摘Arsenic-contaminated water is a serious hazard for human health. Plankton plays a critical role in the fate and toxicity of arsenic in water by accumulation and biotransformation.Spirulina platensis(S. platensis), a typical plankton, is often used as a supplement or feed for pharmacy and aquiculture, and may introduce arsenic into the food chain, resulting in a risk to human health. However, there are few studies about how S. platensis biotransforms arsenic. In this study, we investigated arsenic biotransformation by S. platensis. When exposed to arsenite(As(Ⅲ)), S. platensis accumulated arsenic up to 4.1 mg/kg dry weight.After exposure to As(Ⅲ), arsenate(As(Ⅴ)) was the predominant species making up 64% to86% of the total arsenic. Monomethylarsenate(MMA(Ⅴ)) and dimethylarsenate(DMA(Ⅴ))were also detected. An arsenite S-adenosylmethionine methyltransferase from S. platensis(Sp Ars M) was identified and characterized. Sp Ars M showed low identity with other reported Ars M enzymes. The Escherichia coli AW3110 bearing Spars M gene resulted in As(Ⅲ) methylation and conferring resistance to As(Ⅲ). The in vitro assay showed that Sp Ars M exhibited As(Ⅲ) methylation activity. DMA(Ⅴ) and a small amount of MMA(Ⅴ) were detected in the reaction system within 0.5 hr. A truncated Sp Ars M derivative lacking the last 34 residues still had the ability to methylate As(Ⅲ). The three single mutants of Sp Ars M(C59S, C186 S, and C238S) abolished the capability of As(Ⅲ) methylation, suggesting the three cysteine residues are involved in catalysis. We propose that Sp Ars M is responsible for As methylation and detoxification of As(Ⅲ) and may contribute to As biogeochemistry. | Yuqing Guo Ximei Xue Yu Yan Yongguan Zhu Guidi Yang Jun Ye | 2016 | Journal of Environmental Sciences2016，28，11： | 5 |
| 2 | Speeiation analysis of Arsenic in seafood with capillary Eleetrophoresis-UV detection显示文摘 | Yang Guidi Zheng Jinping Huang Hongxia | 2009 | Chin J Anal Chem2009，37，4： | 1 |
| 3 | Property of nano-SiO2/urea formaldehyde resin显示文摘 | Qiaojia Lin Guidi Yang Jinghong Liu | 2006 | Frontiers of Forestry in China2006，1，2： | 1 |
| 4 | Speciation analysis of selenium in rice samples by using capillary electrophoresis-inductively coupled plasma mass spectrometry显示文摘 | YunQiang Zhao JinPing Zheng MingWei Yang GuiDi Yang YongNing Wu FengFu Fu | 2011 | Talanta2011，，3： | 1 |
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