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| 1 | De novo assembly of a Chinese soybean genome显示文摘Soybean was domesticated in China and has become one of the most important oilseed crops. Due to bottlenecks in their introduction and dissemination, soybeans from different geographic areas exhibit extensive genetic diversity. Asia is the largest soybean market; therefore, a high-quality soybean reference genome from this area is critical for soybean research and breeding.Here, we report the de novo assembly and sequence analysis of a Chinese soybean genome for 'Zhonghuang 13' by a combination of SMRT, Hi-C and optical mapping data. The assembled genome size is 1.025 Gb with a contig N50 of 3.46 Mb and a scaffold N50 of 51.87 Mb. Comparisons between this genome and the previously reported reference genome(cv. Williams82) uncovered more than 250,000 structure variations. A total of 52,051 protein coding genes and 36,429 transposable elements were annotated for this genome, and a gene co-expression network including 39,967 genes was also established. This high quality Chinese soybean genome and its sequence analysis will provide valuable information for soybean improvement in the future. | Yanting Shen Jing Liu Haiying Geng Jixiang Zhang Yucheng Liu Haikuan Zhang Shilai Xing Jianchang Du Shisong Ma Zhixi Tian | 2018 | Science China(Life Sciences)2018，61，8： | 11 |
| 2 | A complex structure of arrestin-2 bound to a G protein-coupled receptor显示文摘Arrestins comprise a family of signal regulators of G-protein-coupled receptors(GPCRs),which include arrestins 1 to 4.While arrestins 1 and 4 are visual arrestins dedicated to rhodopsin,arrestins 2 and 3(Arr2 and Arr3)areβ-arrestins known to regulate many nonvisual GPCRs.The dynamic and promiscuous coupling of Arr2 to nonvisual GPCRs has posed technical challenges to tackle the basis of arrestin binding to GPCRs.Here we report the structure of Arr2 in complex with neurotensin receptor 1(NTSR1),which reveals an overall assembly that is strikingly different from the visual arrestin–rhodopsin complex by a 90°rotation of Arr2 relative to the receptor.In this new configuration,intracellular loop 3(ICL3)and transmembrane helix 6(TM6)of the receptor are oriented toward the N-terminal domain of the arrestin,making it possible for GPCRs that lack the C-terminal tail to couple Arr2 through their ICL3.Molecular dynamics simulation and crosslinking data further support the assembly of the Arr2‒NTSR1 complex.Sequence analysis and homology modeling suggest that the Arr2‒NTSR1 complex structure may provide an alternative template for modeling arrestin–GPCR interactions. | Wanchao Yin Zhihai Li Mingliang Jin Yu-Ling Yin Parker Wde Waal Kuntal Pal Yanting Yin Xiang Gao Yuanzheng He Jing Gao Xiaoxi Wang Yan Zhang Hu Zhou Karsten Melcher Yi Jiang Yao Cong X.Edward Zhou Xuekui Yu H.Eric Xu | 2019 | Cell Research2019，29，12： | 4 |
| 3 | Fabrication of gelatin methacryloyl hydrogel microneedles for transdermal delivery of metformin in diabetic rats显示文摘Injection therapy for diabetes has poor patient compliance,and the pain occurring at the site of subcutaneous injections causes significant inconvenience to diabetic patients.In this work,to demonstrate the benefits of an alternative drug delivery technique that overcomes these issues,methacrylated gelatin hydrogel-forming microneedles integrated with metformin were developed to adjust blood glucose levels in diabetic rats.Gelatin methacryloyl microneedles(GelMA-MNs)with different degrees of substitution were successfully prepared by a micro-molding method.The resultant GelMA-MNs exhibited excellent mechanical properties and moisture resistance.Metformin,an anti-diabetic drug,was further encapsulated into the GelMA-MNs,and its release rate could be controlled by the three-dimensional cross-linked network of microneedles,thereby exhibiting sustained drug release behaviors in vitro and implying a better therapeutic effect compared with that of subcutaneous injection in diabetic rats.The drug release period could be significantly prolonged by improving the cross-link density of GelMA-MNs.The results of hypoglycemic effect evaluation show that the application of GelMA-MNs for transdermal delivery in diabetic rats has promising benefits for diabetes treatment. | Zhiyong Zeng Guohua Jiang Tianqi Liu Gao Song Yanfang Sun Xueya Zhang Yanting Jing Mingjia Feng Yufei Shi | 2021 | Bio-Design and Manufacturing2021，4，4： | 4 |
| 4 | Arabinose substitution degree in xylan positively affects lignocellulose enzymatic digestibility after various NaOH/H 2 SO 4 pretreatments in Miscanthus显示文摘 | Fengcheng Li Shuangfeng Ren Wei Zhang Zhengdan Xu Guosheng Xie Yan Chen Yuanyuan Tu Qing Li Shiguang Zhou Yu Li Fen Tu Lin Liu Yanting Wang Jianxiong Jiang Jingping Qin Shizhong Li Qiwei Li Hai-Chun Jing Fasong Zhou Neal Gutterson Liangcai Peng | 2012 | Bioresource Technology2012，，： | 1 |
| 5 | Morphine attenuates frustration-like behavior induced by sucrose reward deprivation in rats显示文摘In this study,a T-maze-based frustration model in rats was established using sucrose-reward de-privation.The results revealed that rats maintained a 75% preference for the sucrose-reward arm in the reward phase.During the sucrose-deprivation frustration phase,both the preference for the sucrose-deprivation arm (62.5%) and time spent waiting in the sucrose-deprivation arm decreased.Acute injection of morphine increased the preference in a dose-dependent fashion,and prolonged the waiting duration in the sucrose-deprivation arm.These findings indicate that morphine specifically inhibited the frustration response induced by sucrose reward deprivation.To further elucidate the pharmacological mechanisms involved,the opioid receptor antagonist naloxone was given to model rats prior to the injection of morphine.The results revealed that naloxone administration markedly attenuated the anti-frustration-like effects of 3 mg/kg morphine treatment.These findings suggest that morphine attenuates the frustration-like response to reward deprivation in rats through the opioid receptor. | Qing Liu Wangjun Qin Min Zhang Yanting Wang Li Jing Junxu Li Jianhui Liang | 2011 | Neural Regeneration Research2011，6，28： | 0 |
| 6 | Modes and Approaches of Promoting Rural Rejuvenation through the Integration of Industry-Universities-Research Institutions显示文摘Taking the Zhejiang Academy of Agricultural Sciences as an example,the modes and approaches of combing industry-universities-research institutions to promote rural revitalization by agricultural scientific research units are discussed.The main approaches are as follows:focusing on practical technical training to promote the transformation of scientific and technological achievements,transferring scientific and technological achievements to promote the deep integration of basic research,applied research and achievement transformation,and using the agronomist college as a carrier to promote the integrated development of industry-universities-research institutions and promote rural revitalization through systematic personnel training. | Yanting LU Shenghai YE Guofu ZHU Liehong WU Huasheng YU Rongrong ZHAI Jing YE Xiaoming ZHANG | 2020 | Asian Agricultural Research2020，12，3： | 0 |
| 7 | Discovery of a subtype-selective, covalent inhibitor against palmitoylation pocket of TEAD3显示文摘The TEA domain(TEAD)family proteins(TEAD1-4)are essential transcription factors that control cell differentiation and organ size in the Hippo pathway.Although the sequences and structures of TEAD family proteins are highly conserved,each TEAD isoform has unique physiological and pathological functions.Therefore,the development and discovery of subtype selective inhibitors for TEAD protein will provide important chemical probes for the TEAD-related function studies in development and diseases.Here,we identified a novel TEAD 1/3 covalent inhibitor(DC-TEADin1072)with biochemical IC50 values of 0.61±0.02 and 0.58±0.12μmol/L against TEAD1 and TEAD3,respectively.Further chemical optimization based on DC-TEAD in 1072 yielded a selective TEAD3 inhibitor DCTEAD3 in03 with the IC_(50) value of 0.16±0.03μmol/L,which shows 100-fold selectivity over other TEAD isoforms in activity-based protein profiling(ABPP)assays.In cells,DC-TEAD3 in03 showed selective inhibitory effect on TEAD3 in GAL4-TEAD(1-4)reporter assays with the IC50 value of1.15μmol/L.When administered to zebrafish juveniles,experiments showed that DC-TEAD3 in03 reduced the growth rate of zebrafish caudal fins,indicating the importance of TEAD3 activity in controlling proportional growth of vertebrate appendages. | Tian Lu Yong Li Wenchao Lu TWGM Spitters Xueyu Fang Jun Wang Simian Cai Jing Gao Yanting Zhou Zhe Duan Huan Xiong Liping Liu Qi Li Hualiang Jiang Kaixian Chen Hu Zhou Hua Lin Huijin Feng Bing Zhou Christopher L.Antos Cheng Luo | 2021 | Acta Pharmaceutica Sinica B2021，11，10： | 0 |
| 8 | PTEN-induced putative kinase 1 regulates mitochondrial quality control and is essential for the maturation of human induced pluripotent stem cell-derived cardiomyocytes显示文摘Human induced pluripotent stem cell-derived cardiomyocytes(hiPSC-CMs)have attracted attention in the field of regenerative medicine due to their potential ability to repair damaged hearts.However,the immature phenotype of these cells limits their clinical application.Cardiomyocyte maturation is accompanied by changes in mitochondrial quality.PTEN-induced putative kinase 1(PINK1)has been linked to mitochondrial quality control.However,whether the changes in mitochondrial quality in hiPSC-CMs are associated with PINK1,and the impact of PINK1 on hiPSC-CMs development are not clear.In this study,we found that knockdown of PINK1 in hiPSC-CMs resulted in mitochondrial fragmentation and impaired mitochondrial functions such as mitophagy and mitochondrial biogenesis.PINK1 deletion also inhibited the maturation of hiPSC-CMs,reverting them to a naive structural and functional state.We found that restoring the mitochondrial structure did not completely rescue the mitochondrial dysfunction caused by PINK1 deletion,while activation of PINK1 kinase activity using kinetin promoted mitochondrial fusion,increased the mitochondrial membrane potential and ATP production,and maintained the development and maturation of hiPSC-CMs.In conclusion,PINK1 regulates the mitochondrial structure and function of hiPSC-CMs,and is essential for the maturation of hiPSC-CMs. | Huiwen Liu Yanting Sun Hao Xu Bin Tan Qin Yi Jie Tian Jing Zhu | 2023 | Genes & Diseases2023，10，5： | 0 |
| 9 | Pyrocincholic acid 3β-O-β-D-quinovopyranosyl-28-O-β-Dglucopyranoside suppresses adipogenesis and regulates lipid metabolism in 3T3-L1 adipocytes显示文摘Obesity is crucially involved in many metabolic diseases,such as type 2 diabetes,cardiovascular disease and cancer.Regulating the number or size of adipocytes has been suggested to be a potential treatment for obesity.In this study,we investigated the effect of pyrocincholic acid 3β-O-β-D-quinovopyranosyl-28-O-β-D-glucopyranoside(PAQG),a 27-nor-oleanolic acid saponin extracted from Metadina trichotoma,on adipogenesis and lipid metabolism in 3T3-L1 adipocytes.The 3T3-L1 pre-adipocytes were incubated with vehicle or PAQG for 6 days in differentiation process.PAQG significantly reduced the adipogenesis,adiponectin secretion and the expression level of key transcription factors related to adipogenesis,such as PPARc,C/EBPb,C/EBPa,and FABP4.Moreover,PAQG increased the levels of FFA and glycerol in medium and reduced TG level in mature adipocytes.Interestingly,PAQG not only promoted the activation of AMPK and genes involved in fatty oxidation including PDK4 and CPT1a,but also inhibited those genes involved in fatty acid biosynthesis,such as SREBP1c,FAS,ACCa and SCD1.In conclusion,PAQG inhibits the differentiation and regulates lipid metabolism of 3T3-L1 cells via AMPK pathway,suggesting that PAQG may be a novel and promising natural product for the treatment of obesity and hyperlipidemia. | Li Peng Yanting Lu Yuhui Xu Jing Hu Fang Wang Yumei Zhang Wenyong Xiong | 2017 | Natural Products and Bioprospecting2017，7，3： | 0 |
| 10 | A temperature-regulated bioorthogonal reaction to target lysine:Hemiacetal pharmacophore in genipin irreversibly binds with UCP2,inhibiting mitochondrial thermogenesis显示文摘Mitochondria are essential for eukaryotic life as powerhouses for energy metabolism. Excessive mitochondrial hyperthermia and reactive oxygen species(ROS) production have been associated with aging, cancer,neurodegenerative diseases, and other disorders. Uncoupling protein 2(UCP2) is the effector responsible for regulating cellular thermogenesis and ROS production via dissipating protons in an electrochemical gradient. A UCP2 inhibitor named genipin(GNP) is being researched for its effect on mitochondrial temperature, but little is known about its mechanisms. This study developed several molecular probes to explore the interactions between GNP and UCP2. The result indicated that the hemiacetal structure in GNP could selectively react with the ?-amine of lysine on the UCP2 proton leakage channel through ringopening condensation at the mitochondrial, cellular, and animal levels. A notable feature of the reaction is its temperature sensitivity and ability to conjugate with UCP2 at high fever as lysine-specific covalent inhibitors that prevent mitochondrial thermogenesis. The result not only clarifies the existence of an antipyretic properties of GNP via its irreversible coupling to UCP2, but also reveals a bioorthogonal reaction of hemiacetal iridoid aglycone for selectively binding with the ?-amine of lysine on proteins. | Fukui Shen Wen Yang Kaixue Zhang Yanting Jiao Jing Cui Yuanyuan Hou Gang Bai | 2023 | Chinese Chemical Letters2023，34，10： | 0 |
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